Document 3QXJQGe0oYqnnykNobR5V2JEa

ARllirOIIS -"~ ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95 or as part of the mixed product FM-3820 (see Remarks). (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test sample is FM-3820, a mixture of the test substance in diethylene glycol butyl ether and water (approximately 24-28% test substance in diethylene glycol butyl ether and water). Calculations were made to adjust test values using the upper limit concentration of the test substance (28%) in the test sample and no adjustment was made for the presence of the diethylene glycol butyl ether or water when noted below. These calculations assumed that all toxicity was due to the presence of the Perfluorooctanesulfonate substance. METHOD Method: OECD 202 Test type: Static acute GLP: Yes Year Completed: 1991 Species: Daphnia magna Analytical monitoring: DO, pH, Conductivity, and temperature were monitored daily. Statistical methods: EC50 values calculated, when possible by standard statistical techniques (Stephan, 1983) Test daphnid source: Obtained from cultures maintained by EnviroSystems Division, Resource Analysts, Inc., Hampton, NH. Test daphnid age at study initiation: < 24-hours Test conditions Dilution water: Well water from wells at EnviroSystems in Hampton, New Hampshire. Dilution water chemistry: pH: 7.8* Conductivity: 1200 umhos/cm* TOC: <2.0 mg/L * Values measured at time of test. Lighting: Cool white fluorescent lights, intensity 23 uE/s/m2. Photoperiod of 16-hours light, 8-hours dark. No transition period noted. 001G03 Stock and test solutions preparation: A primary stock solution was prepared in dilution water at 1000 mg/L. The primary stock was proportionally diluted with dilution water to prepare the five test concentrations. Exposure vessels: 250 mL plastic beakers containing 200 mL of test solution. The approximate depth of test solution was 6 cm. Number of replicates: Four Number of daphnids per replicate: Five Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 48 hours): 8.2 - 8.5 mg/L (control exposure) 8.1 - 8.5 mg/L (150 mg/L exposure) pH range (0 - 48 hours) 7.8 - 8.6 (control exposure) 7.8 - 8.6 (150 mg/L exposure) Test temperature range (0 - 48 hours) 20.8 - 21.0C (control exposure) 20.7 - 20.9C (150 mg/L exposure) Conductivity range (0 - 48 hours) 1200 - 1300 umhos/cm (control exposure) 1200 - 1300 umhos/cm (150 mg/L exposure) Element basis: mortality RESULTS Nominal concentrations: Bk control, 25, 40, 60, 100, 150 mg/L Element values: 24-hour EC50 = >150 mg/L (C.l. not calculable) 48-hour EC50 = 49 (43-56) mg/L Perfluorooctanesulfonate concentration adjusted Element value: 24-hour EC50 = >42 mg/L 48-hour EC50 = 14 mg/L All element values based on nominal concentrations Biological observations: Ninety five percent survival occurred in the control exposure. The number of surviving organisms and the occurrence of sublethal effects and immobilization or other sublethal effects were determined visually and recorded initially and after 24 and 48 hours. 001604 Cumulative percent mortality: Nominal Test Cone., mg/L 24-hours 48-hours Neg. Control 0 5 25 0 0 40 5 25 60 0 25 100 0 100 150 20 100 Control response: satisfactory CONCLUSIONS The test substance 48-hour EC50 for Daphnia magna was determined to be 49 mg/L with a 95% confidence interval of 43-56 mg/L. If you assume all toxicity of the mixture is due to the Perfluorooctanesulfonate, the adjusted 48-hour EC50 value is 14 mg/L (49 mg/L X 0.28). Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Additionally, data is for a mixture and toxicity cannot be positively attributed to Perfluorooctancesulfonate as the diethylene glycol butyl ether could also contribute to the toxicity. The basic water quality parameters (hardness, alkalinity and calcium/magnesium ratio) were not included in the final report. REFERENCES This study was conducted at EnviroSystems Division, Resource Analysts, Incorporated, Hampton, NH at the request of the 3M Company. OTHER Last changed: 5/3/00 001605 08/ 19/1991 EN/IRO-MENTAL LABORATORY FINAL REPORT 1 LAB REQUEST NO. H2864 DRPDDREEAEQPOSTAUCEJREE:CTRSMTFTEOCCENRNE-2OITNV0::AE3M05D5&9E: 31: 5C81EC31AM4/00PR90C0E/N10IEN9N9ET0RS/SAB SAMPLE DATE CCDE DESCRIPTION COED3MCXCANNPTETEIROR-ALACCCQPHTTPOLDLLUEAAARTBT3SH:E:OD:E0O: 0SS03IT8/:0/15$9/11/219,99091010 .0 0 RESULT MDL CR (C .I.) 1 RM 3017 -------------------- DAPHNIA ACOTE TEST COPIETE 2 FM 8887 -------------------- DAPHNIA ACOTE TEST OOPIEIE 3 EM 3021 -------------------- DAPFWIA ACOTE TEST OOPIETE 4 FM 27513 ------------s-------- DAPfNIA ACOTE TEST COPIEIE 5 EM 4115 -------------------- DAPH4IA ACOTE TEST COPIETE 6 EM 3820 -------------------- DAPSNIA ACOTE TEST OOPLETE 7 FM 55040 -------------------- DAPfNIA ACOTE TEST COPIETE 8 FD 199 -------------------- DAPHNIA ACOTE TEST COPIETE 9 FC 203 -------------------- DAPH4IA ACUTE TEST COPIETE 10 VW 160390 -------------------- DAPH4IA ACUTE TEST COPIETE * - OCNTRACT LAB SUEMITTED BY DATED: tm AVAILABLE