Document 3QG94Mky8y6GRxOG2xGzO6k93

IFEB-I3-2006 07:IIP FROM: TO:161825908= 112 SILICOSIS AND OIHLR FIBROT1C PNtUMOCONlOSES (7) Very early farms unsegmemed (" sausage-shaped "). (8) Later forms crenated, segmented, or resembling a senes of oval discs, or beads strung together in a necklace form. Sometimes, later, a short lenglh of fibre is seen between the segments. (9) Bulbous or pointed extremities (or one bulbous and one pointed suggesting " heads and tiuls"). Simson and Siruchun (1931) have noted sharply nnglcd forms suggesting incipient fracture, and beheve that the tapenng tail forms may be the broken ends of this fracture (10) Generally quite straight, occasionally curved, very rarely S-shaped During examination in a wet preparation, slight pressure on the cover slip will sometimes cause them to bend owing to tho elasticity of the central fibre. This proneness to bend under stress and strain may explain the curved forms a Ben seen in sections of tissuo, os suggested by Gardner and Cummings. (11) Forms seen " end-onlook like knobs or door-handles. Small secondary knobs and bosses sometimes seen thereon. Histological technique. - -Gloyne (1933) describes the following technique for the identification of these bodies: After digesLion of the sputum with equal quantities of concentrated antiformin and centrifugation, the antiformin is pipetted off, and the deposit covctcU with a small quantity of a 5 per cent solution of ammonium sulphide The asbesiosis bodies are then coloured blnok. This colour cun he removed with hydrochloric acid. Sections. --In the case of sections the following technique has been found useful: After removal of the paraffin wax with xylol and alcohol in the usual way, the secUon is washed with water and then flooded with ammonium sulphide, which is allowed to remuin for several minutes. The section is next washed and counter-staiaed with an aqueous solution of neutral red, dehydrated, and mounted in balsam. This method of staining also shows (he presence of a large amount ofiron in the sections. Unfortunately the black colour is slowly removed by the mounting fluid, and the sections will only keep for a few weeks. Staining with haematoxylin--Haomatoxylin, which also has an affinity for iron, can be made to colour asbestosis bodies, but it is rather a deposition of stain than a true staining reaction. The technique is as follows. After digestion of ihe sputum with equal quantities ol concentrated unlifarmin and after centrifugation, the antiformin is pipetted off and replaced by a S per cent solution of Ehrlich's haematoxylin. Blueing or Lhe hnemtuoxylin immedi ately takes place owing to the remains of the alkaline anliformin. The mixture is well shaken and allowed to stand for -1 hour, and then centrifuged again and the deposit mounted as a wet preparation By this means the asbestosis bodies become a dark-brown or black colour, according to the length of time they have been exposed to the hoemuloxylin. Although these haematoxylm-coloured bodies give the impression of having the haematoxylin deposited on them rather than having actually taken up the stain, after washing over-night they still retain sufficient stain to give a darkbrown effect under transmitted light. Pi10'13 Diagnosis of asb While the me general--naxnclj gulion--the rda Occupational his Reference is n our knowledge that time the in good, and neithi and the process? was heavy Th advanced stages graphic change standards the tc result that radlc conditions have themselves for d the disease has experience of the in the earliest si Another matt asbestos-mattres females, but doi associate dust d This is further manifest until tl is then deslgna months--is son and leads to in changes in asbe lung fields, andt ally during mei simulate them w However, by i in the main cer diagnosis to the Nevertheless, cv possibility, pro\ the manner desc Clinical aspects . Dyspnoea is i spicuous becausi I.M. Vnl, III-*