Document 3Q9QgMgboEDpoBDzjJaJEqVOa

AR 226 _ OS TH SUMMARY OF A STUDYSUCLOFNODNUACTTEE(DPFWOIST) HATPODTUAPSOSNITUM PERFLUOROALKYL Type: Biopersistence Screening Study Species/Strain: Sex/Number: ~~ RMaatles//SC/rgrlo:uCpD.SD(IGS)BR Exposure Period: Frequency of Sor 10 days; 94 daysofrecovery Treatment: Exposure AAdmmloibnitiuummfoprerSfloruo1ro0odcatyasnoate: 20 mg/kg Levels: Method: SPioxtagsrsoiuupms poefrfraltusorwoearlekyaldsmuilnfiosntaetreed(PaFmOmS)o:ni1u0mmg/kg 5pecrofnlsueocrouotcitvaendoaatyes,a1ndgrtohuepotrheecrei5vegdrotuhpestersetcseuibvsetdatnhceetfeosrt bsulbosotdawnaces fcoorll1e0ctdeadysf. roAmpeparcohxirmaattferloym 2thheougrrsouapftdeerstihgenafitrestd dfoosre, 2dohseosu.rsTphoesst-edossaimneg,raatnsdwbelroeodsaacrnidfilcievderosnwteerset dcaolyle5ctaepdp.roTxhiemately wreemraeisnaicnrgifgircoedupasndrehcaedivbeldotohdeatnesdt lsiuvbesrstacnoclelefcotred10ondatyess.t dFaiyvse 1r0ats of(2thhoeurbslopoodstw-daossed)e,te1r3,mi2n4e,d5b2,yoursi9n3g/9a4.WiTchkebotlotdaltofrlcuhorine content sceolmebcutisvteioelnecmtertohdoe.d, Bfoodlylowweeidgbhytsanaanldyscilsinwiicatlh saigfnlsuowreidree iroencorded throughout the test. Liver weights were recorded, but not reported. sSuilxfognraotuepsasodfersactrsiwbeedreabaodvmei.nisFtoeordedapnodtwaastseirumweprereflauvoairloaabllkeylad alimbmitounmituhmropuegrhfoluutotrhoeoctetsat.noCaeortnesotiglrwouapss.used as the vehicle for the GLP: Test Substance: sPuostpaesnsdiiunmgpietrifnlucoormoaolily.l Tsuhlefornaattieo wofasacdeitsosnoelvteodcionrnacoeiltownaesbefore 20:80. tested asNedgeastcirvibeecdonatbroovles. of com oil and corn oil/acetone were also No Ammonium perfluorooctanoate, purity 93-97% Results: #P2o1t9a5s-s3i9u-m3,pe3rf-l8u%orCoaAlkSyl#3s8u7lf1o-n9a9t.e6,,pu3r7it%y 8C2A-S86#%29C4A20S-49.3, 1-3% CAS #60272.25-1 pReatrsindeousmedanwditdhiaarmrhmeoandiuurimngpetrhfeludoorsoioncgtpaenroiaotde. exAhliobpietceidawwetas eoqbusievravleedntdsur(ibnlgootdheorrgeacnoovfelruyorpiedrieodle.veTlsh)einnorramtablliozoedd uM peaked after 2 Reference: 8T3.h3deadyaCsyms.oafxTdowhsaeisnAg5I1a(8nA.d1c2ctuh+menu4l4da.et8ci9roepnapsIemnddwetixht)rhowuaagstheorumtintael theasltfi-nlgiepeorfiod. L(oBiionfaicnciutym)ul(aAtUiConI)NFw/asD)64w9a7.s5.calAcunlaatreedauannddenr1ot2rh.m5ealacinuzdrevtdehef(oeBsItimated fluorine content. The AUCINF/D was calculated as 70.79. odRfaatmvsahrediaoo,suessdaplawriitvtsahtoopftno,htaeaslsobpioeudcmyiapd,eurbrflilanucgokrtoohacelukdlyoalsrisdnuiglsfcpohenarartgee,exahnidbistteadining eaqusivoablseenrtsve(dbldouordinogrgtahenorfelcuoovreirdye rise throughout the dosing period. levels) The norimoda.lizAeldopuevcia in rat blood continued to hsatlefadlyi-fsetaotfe4.0.T5hedayCsm. axThweapAselr9i8wo9ad.s8a55n9d.0m1aa1yn6d.n9ot0htephpBamIvewwarietsaha5ch8e3tde8r2m.inal `The AUCINF/D was calculated as 566,479.1. NNou.bo2n9t22C.o. (2000). Unpublished Data, Haskell Laboratory Report SUMMARPYEORFFLSTUUODRIOEOSCCTOANNDOUACTTEEADTWDIUTPHOANMTMONIUM Ecotoxicity Type: Value: Breakdown products: Method: GLP: ReTseuslttSsu:bstance: B1i3o%deagt rDaadyat2i8on co, iMwnoaodsciumfleiuaemsd.uSrterduamsTCeOst;(eOvoElCutDio3n0.1 ABc)t.ivIantehdissltuedsg,ebwiaodsegursaeddab2isltihtyy No RAAemmammdooynniiBiuuommdeppgeerrraffdllauubooirrlooiootccytt.aa"nnooNaaotttee,e(pt3uh0rait%tfysaoaillsuustriueomtneo)dpdai1sd0sn02o%t"Rdeemonstzate pBeirosdiesgtriandatbhieleintvyiTreosntm"ednotefsornoatniemxptleyndtehadtptehreiotedstofsutbismtea.nacdeywill rTehfeerteonxciecicthyetmeistc,alwh(iscohdiiunmclbuednezdobaotteh) < 25% biodegradation within 14 days; the test substance itnhetrheefosrae,meamflmaosk, and the yielded tpheerfilnuoocruoloucmt,anoate was considered inhibitory to nium microorganisms in 23 Reference: NRou.bo2n4-t9C7.o. (1997). Unpublished Data, AEM LaboratorRyeport MTeytpheo:d: SAAtdmusdmoiorespntwiieuornme-ppDeeerrsffolorurpomtreoidoocnotnaSncsoreaevteeernaaildnstgoersStpttmuiadotinee/rsidaelsso,rptionscreening aDcslesaloyaspw,eaarrefw,oarsUmhSeedAd.ussaTinnhdge,tpawedoastormpotsison,/adensdorapntaigornicsuclrteuerinanilcnslgouisdltiufndgriaewmse,were WOohiroksRipvlearntcnoellaercPteadrkienrsthbeusruvgri,fcaiWcneeitssytooilVfsirtaghinendDiatu.wPoonstedWiamsehnitnsgfiroonm te PaTedhsaetpitaceidddseofsrrpaotnimdoanT/modexetsiohcroSpdtuibdosentvapenrlcooecpseedd(uOrbPeyPutThsSee)d,OfifUnintcihetiesodfstSPturdoyvewnatsion, p{EeunsvstiiErcoiPdnAemse(na1tn9ad9l8t)Po.rxioFactteesc,utbitsortnaannAscgpeeosrn,tcayndfotrheusdeeivneltohepmteesnttianotgfeostfest data P`irsOeovPtehPneStrimo8,n3U,5n.Pi1ets2e2d0SstaetdeimEennvtiraonndmseonaitnladaldtsrPoraronptsteficootrnim/oadnteisAoognreptntecisoytn:g.oiOdfefliicneeosf: ticides, and Toxic Substances, EPA7I2-.98.045), amEbexsscoehrp)pttsitfoaonir/ndtlehessesocrslptateyieslo,nsaislecvsreeaeminnpilpnergseptweasertar.teiFosuncrrtfehoeernretmhdoerteh,rough a 2-mm (10 suwtsaaesidnhleiendsstuhssetiesnetgludDsiyIe.v(eTdseh.ieoMnsaiatzneedrd,i)aswloiarltseetraitnherdoougnht2he-t5u0muamnthdesSise0av-neudwmwaass eestrruedeiedbseatsweeerdrmeoinnaiorv-edrnieddrypriwoerig0htussoe.f, taDhnaedtassaecmdapillcmeuesln.attsiOouvnssee,nd-hdionrwyetvhweeeri,ghts 105C for a miendiamfutemrodfry2i4nghoaurssu.bsample of material in ap aves : sTuhbestpaHncoefttohewastaerm)p.lePserwcaesntdeotregranmiicnemdatitnear the loss on ignition method. rwaatisoodfeter1m: itneesdt using Screening Test: Adsorption Studies afblmhaemnokandcsioonurtmpatiipnoeinrnfgtleu0sot.rw0oa1oscMtpCaenaorCaflto,ersmaoenlddutiainosdniunwpgillitechcaottenesttoronmaetaercihalmsatearnidaln,p A pashmoamseoinnocilfauudmseodpl.eurtEfilaouncohorfomoatcthteearnioalatweacsonecqeunitlriabtriatoendbwuittnhlotahtmeaetaaeqcruhiaelouwsere test substance at 50, 500, and 5500, ng/L oy prepared in 0.01M CaCl, GLP. ReTseustltSusbstance: VSetsesreillse.50OnmeLppaortlyapirr-odpryileednmeacteenrtiarlif(u4g.e0tgu)bewsaswewreeiugsheedd aasndthe test ee5nxpdca-eropttvsetrthe-esctcnsodonltmuritoxileonrtuba(e2n.0d maTgLhi)etacwteeendrteartidfaeupcgpaernottxuebidemsiantwteoelrycee3nst0ercriufprumgeedfotorunbaes, ca2en4ndthrtoihuferusng.efitSlutabemerp.edleTtshhrewoeuvrgoehlucaem0ne.t2roiffuamuqgeundeyolfuoosrn 2ssu0yprmeiirnnngauettfaeinsltteawrtai4sn0t0om0eaaxneg,w caondmproefurnidgeruastiendgaatnaLppCr-oMxSim.atTehley 5blaCnukntainldatnhaelyczoentdrofolrttuhbeespaswrueerrneted subjected 0 the same steps as the test systems, including filtering. Sereening Test: Desorption Studies msAiuxbfesrtdea,snhccveenowtlrauismfueagodefdd,e2d0fitlmoteLeraedoc,fhs0st.oolr0ie1ddM,pahCanasdCela,(npaselloyllezutet)id.oanTshwweiatsshoadumtopplteehewinatses.t Ntthioemea,drseosruplttiionng sitnutdiweos.waTshhiisndgessothrapttiwoenrestaenpawlayzserdespeepaatreadte3lsyecond cFAhimavrmeacootnef9riiusmtmaitcepsreioraffllasumotrmeosootcnetdiaufnoomratptehe,erfpaludursoiortroypotnciototanna/opdapetlseiocr(appbteliaeotnmoss, cUlpasyt)reexahmibsietdeidmseingtn,ifEiacsatntWaodosodrpstoiilo,nG(a>r2de5n%oArfeaamsmooi,nianudmKaolin cpoenrcfelnutorraotoicotnasnooaftaemamddoendi)uamt opneerfolruomroorocetoafntohatee.adSdaendd, anMototanntamydoscrooirnlbcleoanntsiritagetniicoflnia.cya,ntDoawmnousnttreoafmasmemdoimneinut,m apnedrfSlausosraoforcatsansooialtedid KtOhanrolelyeincpoecnalctaeymnotasrdasst,oirohbnoeswdeovafesarid,gdnaeidfdsicoaarmnbtmeodanmaiosuuinmgtnipofeifrcfaalnmutomraoomonocituaunnmtoaatte.all ocpofelralfemlcumtooerdnooaitcutWmaanspoheartifenlgauttoor5no0o0Wcotaarnnkdosa5t0ael0sa0ot uSag0d/0sLo.urgb/TeLhd.esiGUgapnrsifdtiercneaanAtmraemaosuonitls . ssiegdniimfeicnatnst farmoomunthteoOfhaimomRoinveiruamnpdeErafsltuoWrooooedtansooialteadasto5r0bepdg/aL only. pOenrcfeluaodrsooocrtbaendoaotnep(e>at7m6o%s)s,dimdonsott asolution of 0.01M CaCl. For the of the ammonium Kdaeosloirnb calfatye,r tmwoostwaosfhtihen.gs with aaddemdsmeoordbnaeitduSwm0i0tphegr/wfLals,uhohrioonowgcet(va>enr6o,0a%ta)en.aadvsAeotrraabgemedmoowfhn9ei8nu%maddpidederdfnloauttodrSeo0so0oc0rtabgnLca,te 5fw0rhoiumcgh/tLhaeddsKeoasrooblreibdned4cl6oan%ylyaoffatbetrhoeuttwao2m9mw%.aosnhFiiounrgmsth.peerTGfhalerudoEeraonsotActrWaeonaooasdotjelsoa5ldl7,d%edoaft tthhee a50m0mpogn/iLusmolpuetriofnl.uorNooocmteanaosautreabdleesodrebseodrpwthioennofadasmormboendifurmom wpearsfloubosreorovcetdanwohaetenftrhoematmhmeoUnpisutrmepaemrfsleudoirmoeoncttaanfotaertetwwoaswaasdhdiendgs Reference: at50 pg/L. DuPont Co. (2000). Unpublished Data, Report No. EMSE-053-00. Acute Toxicity to Fish Type: SpVeacluiees: Method: 96-hour Lepomis LCs macrochirus (bluegill sunfish) A63r4amngge/fLin(d9in5g%sftiudduyciwalasinctoernvdaulc,t5e6d7-us7i2n5g mg/L) nominal test concentrationsof0, 0.1, 1, 10, 100, and 1000 mg/L. 3F2o8r,t4h1e0d,ef5i1n2i,ti6v4e0s,tu8d0y0,, naonmdin1a0l00temsgt/cLo.nceGnltarsastiaoqnusarwiear(e200,12)62, ccwoehnratemabiaensrissnigginn1e0tdhLueswoiafntgteerrstabnsaodtlohumtuisnoeundmwbteeorrmesa.einmTtpealionnyfecidso.hnswtPeaornsteittaiedomdnpesedorfattofueersatech troetpallic2a0tefiusshinpgerrcaonndceonmtrnautmiobne)r.s C(o2nrterpollicfaitsehsrpaenrgceodncfernotmration; w1e-i2-g2h.t8(emmeasnta0n.d2a2r8d lg)e.ngCtohn(tmroelanlo2a.d1icngm)waasnd0.02.304g5/1L-a0t.5t2es4tg wet cdounrcilnugsitohne. tesFti. shAwpehroetonpoetrfieodd aopfpr1o6xhiomuartsellyig2ht4 (h3o1u2r-s3p4r4ioLrutxo)and versus 8 hours darkness was employed with 25 minutes of . tlirgahntsiitnitoenravlall.ighOtbs(e<r2v.a1t5iLounsx)foprrmeocretdailnigtyanadndfoblelhoawviinogratlheef1fe6c-thsour were made daily. Dreipslsioclatveedbeofxoyrgeenad,dpitHi,onanodf ftiesmhpeatratetsutrestawret,redamileya,sautrteodtailnfeiashch cmoonrtdaulcittiyv,itayndo/forthaet ctoensttreonld.watTeotrawlearlekamlienaistyu,reEdDbTeAforhearfdinsehssw,eraend awadtdeerd-caotnttersotlstraerptl.icAatecownatsinuusoeudsltyo rcehceocrkditnegmptehreartmuormeevtaerriaitnioans cdounrcienngtrtaheti9o6n-shionurwatteset.r wCehreeminoctalpearnfaolrymseeds. of the test substance. GLP: ReTseuslttSsu:bstance: The Yes LCso value and its 95% fiducial interval were calculated, Ammonium Mortality in tpheerfrlaunogreofoicntdainnogatset,upduyraiftiyer9996% hours was 0, 0,0, 0,0, and 80% at 0, 0.1, 1, 10, 100, and 1000 mg/L,respectively. T32o8t,al4m1o0r,t5al1i2t,y6w4a0,s 800,05,, a1n0,d 1150,0300,mg4/0L,.6c3o,ncaenndtr9a5ti%onisn,the 0, 262, respectively. exception of 1 All deaths occurred within death at 48 hours at 1000 24 hours, mg/L. No with the behavioral e1f0f0ec0tsmgw/eLrewenorteedletahta<rg6ic4.0 mg/L. Surviving fish at 800 and Reference: TwAoaltltaeclrhacelomkniatclriaonllitawyne,drEepDh7yT9sAimcgah/laLr.pdanCreaasCsm,eOt3ae,nrds76cwoemnrgde/uLcw.tiiCtvhaiiCtnyOoesfx,pteahcnetdedidlruatniognes. ca1on6nd5ctepnemtmhrpoaetsria/otcnumsr,erarrenasgnpeegdcetdfivrfeorlmyo.m62,71D-4u8-r.i25n2mg,gt1/hLeC,.tpesHt, rdainsgsoeldvfedroomxy6g.9e.n7.4, NDou.Po6n1-t9C4.o. (1994). Unpublished Data, Haskell Laboratory Report Type: SVpaelcuiee:s: Method: 96-hour LCs 4O0n0c1ormhgy/nLch(u9s5m%ykciosnfsi(dreanicneboinwtetrrvoault,) 3327-4932 mg/L) cAonstdautcitcerdanugseifnigndaidniglusttiuodnywwaittehrScofnitsrhoplearncdonncoemnitnraaltion was concentrations of 50, 100, 500, 1000, and 5000 mg/L. N1o0,m0i0n0almgc/onLce(n2t0r%atsioolnustioofn6o2f5,am1m25o0n,i2u5m00,pe5r0f0l0u,oraonocdtanoate in water) were preliminary rcahnogseefninfdoirntghestduedfyi.nitive test based on the results of the T9eLstocfhtaesmtbseorlsutwieonr.e sTtwaionlreespslistceaetle ateqsutarcihaamthbaetrhselwderaeppursoexdimpaetretleyst ccoonncceennttrraattiioonn).wiEthac1h0cfhiashmbinereawcahscchoavmebreerd (wtiottahlaofgl2a0ssfipslhatpeerto ptersetvceonntcfeinsthraftrioomnsestcoatphiengt.estRcahnadmobemrnsuamnbderpossiwteiroen uosfetdestto assign concentrations in the water bath. 7 2R9aihnoburoswptrrioourt tuoseadndindutrhiisngsttuhdeytwesetr, eanndotweferdeaapspsriogxniemdattoeltyhe test scohlaumtbioenrsswuassinignirtaiantdeodmapnpumrboexris.matAedd4liy1timoinnuotfefsisahftteortmhiextienstg of GLP: Test Substance: Results: otbhseetrevsattsioolnustiwoenrsewmaasdceomaptlteetsetds.tarMt,oretvaelriyty24anhdoubreshatvhieoreraaflter, and attest end. At test conclusion, all surviving fish were sacrificed. Ain rtheecitrecsutlacthianmgbweartserdubraitnhgwtahse 9u6s-ehdotuormtaesitn.taIinnamdediatniotn,emaperature cteomnpteirnautouurselyvarreicaotridoinngint1herrepmloimceatteerofwathse udsieludtitooncwhaetcekrfcoorntrol. photoperiod of 16 hours light (approximately 199-450 Lux) and A l8tirgahhnotsuiirtnsitoednrvaaalrlk,lnigehsts (w1a1s-1e5m7plLouyxe)dp,rwecheidcihnginacnldudfeodll3o0wminignutthees1o6f-hour TrDehipeslssieocalmtveeeadcshouaxrmyebgmeeenrn,stopsfHw,tehraenecdotntatekrmeopnlebraeanftdourrteeesftwiesshrubewsemtreaeancsaeudrdceoenddcieannttatrlealsttions. isntarat,coenvceernytr2at4iohno.ursTotthaerleaafltkealri,niatnyd, aEtDteTstAehnadrdonreass,toatanldmorality tchoendbuecgtiinvniitnygofofthtehewtaetset.r wTeesrtesmoelaustiuornesdwbeerfeorneotfiasehrawteerdedaudrdiendg at the test. Cdiorneccetnltyrbaytihoingshopfearfmomromnanicuemlpiqeurifdlucohrrooomcattaongoartaephwye/rteamnedaesrumraedss spectrometry (LC/MS/MS). w2Ae.ti5tge3hs.tt2,cebolmnoctilinuesdsitodanrn,ydaf(rimdsehlafenrngo0tm.h21t(hmeg)e.waantSet2ra.n8cdocanmrtdr)ollaenrndgatn0hg.e1od5f-ft0hr.eo30mlogngienswtet cfoisnhtrwola.s not more Loading than in the twwaitceertchoenlteronlgtwhaosf 0t.h2e3shgo/rLteastttefsitsh in the conclusion. The LCs method. value was calculated using the moving average-angle Yes AAtmmthoeneindumofp9er6flhuoourrosocitnatnhoeatrea,ngpeufriintdyi9ng9.s4tu%dy, the mortality was r0e.s0p.ec0t,i0ve.l0y,. anTeds6t0s%ubastta0n,c5e0,so1l0u0t,io5n0s0w,e1r0e00c,leaanrdan5d00c0olmorgl/eLs,s with no insoluble test substance present during the study. aInmmthoendiefuimniptievreflsutourdoyo,cmtaenaonatmeewaesruere5d54c,on1c0e9n0t,ra2t2i8o0n,s4o5f60, and 9360 for the respectively. 6C25o,nt1r2o5l0s,o2lu5t0i0o,ns50s0h0o,waenddn1o0d,e0t0e0ctmagb/leL dose levels, Reference: Mammalian Toxicity Acute Toxicity Type: SVpaelcuiee:s/strain: Method: csounbcsetnatnrcaetsioolnustioofnasmwmeorneiculemarpaernfdlucoorlooorclteasnsowaitet.h nAollintseosltuble test substance present during the test, Awlilthcihneemxipceaclteadndrapnhgyessi.caTloptaarlaamlektaleirnsitfyo,r EthDeTdAefihnairtdinveestse,stanwdere c1o2n2dumcgt/ivLiCtyaCoOfst,headnidlu2t4io0npwmahtoesr/ceomnt,rorleswpeecrteiv4el9y.mgD/uLrCiangCOtshe, pteHst,rdainsgseodlvferdomox7y.1g.e7n.2c,onacnedntmreaatniotnesmrpaenrgaetdurferwoams7.151-181.C2 (mrealnLg,e 11.6-12.1C) Ncoontmroorltfailsiht.yNorosmuobrlteatlhiatlyeofrfescutbslweethraelseefefnecitns twheerdeiloubtsicornvweadtaetr c1o1n/c2e0n,tr14a/t2i0o,nsan<d21550/020mgat/L2.4,A4t8,57020,0amngd/9L6, mhoourrtsa,lirteyspweacstiv8e/l2y0., At10,000mg/L all fish were dead by 24 hours. sSuucrvhiavsinsgwifimsmhienxgpoastetdhetosu5r0f0ac0e,mgl/abLoreexdhirbeistpeidrastuibolne,tdhaarlk.effects aconldoreartriatoinc, slwetihmamrigny,g.partial loss of equilibrium, rapid respiration, NDou.PoDnutpoCnot.-3(318919.9.). Unpublished Data, Haskell Laboratory Report Oral LDgy M4Ma7al8leemrgraa/ttkssg/rCe(rc9le5i:v%CiDncgonafmimdeonncieulmimiptesr,fllouowreoroc3t6a1nomagt/ekga,lounep:per 571 mg/kg, slope 7.9) pMrael-etrreaattsmreencteoivfipnhgenaombmarobniitaulmspoedrifulmu:or5o4o7ctmango/aktge (a9n5d% confidence limits, lower 517 mg/kg, upper 582 mg/kg, slope 22.1) pMrael-etrreaattsmreencteoivfipnrgoaadmimfeonnihyudmrocphelrofrliudoer:oo5ct2a0nomagt/ekagn(d95% Acomnmfiodneincuemlipmeirtfsl,uloorwooecrt4a5no0amteg/wkags, audpmpienris6t1e8remdg/bkyg,intsrlaogpaest9r.i8c) i1n0truabtast/igornoausp aatscuosnpceennstiroantiionncsoorfn40oi0l,in5s0i0n,gloer d6o5s0esmgt/okg. Another 30 rats, 10/group, were treated by single intraperitoneal injections aTrepst:Substance: Results: afwdoirmtih3niadsqatyueser.oeudOsnasemolmduoatiynoianfustmeorfptpehhreelnfuoionbraaolrobtcirtteaaanltomsnentoted,ibutyhmeianrttart8as0gawmsetgsr/eikcg/day aiindntdtriuatbpiaeotrniiaotlnonguersaoilnugipntjohefect3si0oanrmaeotsf,tar1qe0ua/tegmoreuonsutps,aoswludeteriseocnrtsiroebfaetdpedarbowocisitehf.eannAp aihnymtdmurbooacnthiioluno,rmiudpseeirnafgtluStoh0reomsogac/tmkaeen.otarOteeantewmaehnsotuaardsmaidfnetisesctrtehiirbseeddtrbaebyaotivmneet.nrtag.{ashteric: dvsauulrruivenisvgaiwneg1rr4ea-tcdsaalfycrurolemactoaelvdlertfyrreopametrmtiehonedtmsaonwrdetartlehietwnyesidaagtchar.eifdicaendd. oTbhseer1vepdsy oTanthsealflprh6oe0nmorbbaoastr.hbipthaalssesodwieurmepchoamsbeionfetdhaensdtutdhye wLaDsegrevpaelauteedw.asThbeased TNAhmoemreonweiruemnpoesrifglnaiofriocoacnttadnioftfee,repnucreistyinatphperoLxiDmatveallyue1s0o0f% pfaromolamldooiwnfieinnguhmpyrdpere-ortfcrlheulaootrrmoieodncett.awniotahtee,itchietrhpehrewnhobeanrbtietsatledsaoldoinoemoror pMweoerrafttlaeuldoirwtoiyotcrhtata4in0oo0sa,toef5,020/r,1e0sap,ne7cd/t16i0v5,e0laymn.gd/W8ke/gt10aamwndemlrooenriosbutsameinrevdedpefroirnetahle raartesa aswiengadnkswneoeibsgsshet(r5vl0eo0dssiawnnecdlr6ued5oe0dbsmsegtr/avkiegnd)e.datfaanacdle l(ce5hv0re0losmaoensddtae6cd5.r0yoOmmgth/hkeegar)c,hmicay (500 mg/kg). All desths occurred within 6days fier dosing. Mp5r0oe0rt,arleaainttdeyd6o5wf0it0m1h2ep0/,hke4gn/o2ab0ma,rmbaoinntdail1u9sm/o2dp0eirwufamlsuaonordbosotechretvnaendtortfeeoa,rtetrdheeswpiretachttsi4v0e0l,y. oWlobesstserwavenerddeoiornbcsslteuardiveneedddwapetearakilnlncelaselsveal(rs5e0t,0essattenaddi.n6eOd5t0fhaemcrge,/ckldigina)ircaeahnledss,ilgeantnshdarwgeyight (650 mg/kg). All deaths occurred within 4 days ater dosing. 5pM0roe0rt.traaelanitdteyd6o5wf0it1mh/g1p0/,rkog5a/da1i0mf,emnaonhndyid8ur/om1c0hpleworarfsiiudooebrsaoenorcdvtteahndeofnaotrter,tehareteesrdpaetwcsittihve4l0y0, woSbetsaaeiknrnevedesdsfaicwneec,rlwueedoetbdasntedrr/evomerodrssatta(ia4nl0e0dlempvgee/rlkisnete)ea,sltceadhr.reao,Omtowhdeesircgrhcytlionlrioscwhasl,eaasnigdns 400 mg/kg), diarhea (500 and 650 mglkg), and lacrimation |0 Reference: D(6u5P0onmtg/Ckoe.).(1A9l8l1)d.eaUtnhpsuobclciusrhreedd Dwaittah,inHa5skdealyls Laafbeorrdaotsoirnyg.Report No. 567-81. Type: SVpaelcuiee:s/strain: Oral LDso MMaallee raantsd: upper 536 f4e7m0almeg/raktgs/(C9r5l%:CcDonfidence mg/kg, slope 8.6) limits, lower 403 mg/kg, Method: GLP: ReTseuslttSsu:bstance: Female ras: 482 mg/kg (95% confidence limits, lower 438 mgikg, Tuhppeertes5t4s1ubmsgt/akngc,e,slaospae s1u2s.p3e)nsion in intragastric intubation in single doses corn to 6 goriolu, pwsasofamdamilneiastnedr ed by r6agtsr/ogurposupofwefremeaaldemiynoiusntgeraeddul2t00ra,ts4.00T,e4n50m,al5e00a,nd67f0e,moarle Ta1hn0de00oLbmDsgse/orkvveeadloufdeustrhiwenetgreesatc1sa4ulb-csdutalaayntcreeed.cforvToehrmeytsphueerrvmiioorvdtianalgnidtraytthsdeawnteasraecrwiefiicgehd.ed No MAomrtmalointyiruamtipoesrofflu2o/r1o0o,ct3a/n1o0a,te4,/1p0u,ri6t/y10a,p9p/r1o0x,imaantdel1y0/11000w%ere found in the respectively. 200, 400, 450, 500, Mortality ratiosof 06/7100,,a0n/d101,050/010m,g7//k1g0,m9a/l1e0,graonudps, f1e0m/a1l0ewgerroeupfso,unrdesipnectthieve2ly0.0, 400, 450, 500, 670, and 1000 meg Clinical perineal signs area, woebaskenrevsesd,inanmdalweeirgahtts ilnocssluadteadllstlaevienlesd and/or tested. wet Other c(l4i5n0icmalg/sikggnsanodbsaebrovveed),inchtrhoemmoadlaecrryatosrrihncelau(de5d00staanidne1d0f0a0cemg/kg), cwhliuotnshceihdne4(d6dp7ao0yssmtougr/fekdg(o)5s,0i0nagnm.dg/gkags)p,inmgor(b6u7n0dmiet/yke()6.70Amlgl/dkega)t,hseyoecscuhrarled Reference: Clinical perineal sairgenasaonbdsweerviegdhtinosfsemaatlaellraltesveilnsclteusdteedd. stained and/or Stained face wet was oinbsfeermvaelde artatasllinlecvleuldsedexacleopptec2i0a0(m4g0/0kgm.g/kOgt)h,erwecalkinniecsalss(i4g0n0s,n4o5t0e,d 6c7h0r,omaonddac1r0y0o0rrmhge/kag()6,70humngc/hkegd),poesyteusrheal(f45cl0osaendd 670 mg/kg), (670 mg/kg), and ataxia (670 mg/kg). DuPont Co. (1981). UAlnlpudbelatihssheodccDuartrae,dHwaistkheilnl3Ladbaoyrsatafotreyr dRoespionrgt, No. 295-81 Type: VSapelcuiee:s/strain: Oral LDgy Male Male slope nanedwbfeommalreatsr:at2s4/3Crmlg:/CkDg 55.7) (95% confidence limits, undefined, Method: GLP: Test Substance: Results: F24e4mamlge/knge,wbuoprpnerr2at7s6: 2m58em,g/sklgop(e9519%.9c)onfidence limits, lower M51a4lemgw/ekagn,liunpgperarts6:6547m3g/mkgg/,kgsl(o9pe5%8.c0o)nfidence limits, lower 4F7e0mamlge/kwge,anulpipnegrr2a2ts5:85m8g0/mkgg,/ksgl(o9p5e%4.c7)onfidence limits, lower 4M0a3lemgy/okugn,guapdpuelrt rats: 536 m4g7/0kgm,g/slkogpe(98.56%) confidence limits, lower 4F1e3mamlgekygo,unugppaedrul5t0a3tsm:g/4k5g3, mg/kg slope (95% 15.0) confidence limits, lower Con Male older rats: 336 mg/kg (95% confidence limits, undefined) 2F8e5mamlge/kogld,eurprpaetsr: 343 390 mg/kg mg/kg, (95% slope c8o.0n)fidence limits, lower (Tin1ht0erargtaeatssstt/rsgiurcobusiptn)atnuwcbeear,tieaosmnaailnsesuisanpngedlnesfideoomnsaelisen ntcoeorgwmrbooouirlpn,sowrafatssraa(td<s.m2inTdihasyetsegroreloddu)pbey y(maoaguleneguanndaeddfuilfnteesmda()l.-e8-wM1ea0alnweleiennkegswrboaltodsm)(,2ra1antdsdawmyeasrleoeldaa)dn,mdimnafilesmetaealrneeddolf1de3em0ra,lr2eat0s0, wa2de4ma0in,nl2ii8sn0tg,err3ae3td0s,w13eo0rr,e317a60d0m,mign2/0ik0s,gt.e2r2eF0de,m325a40l0,e,4n208e00w,,b4oo5rr0n,32r5a02t5sm,gw/6er7ke0g.,. oMrale 457051000,,om0grr/6k6g77.00 mmFgge//mkkgag.l.e YYwooeauunnnlgginaaddguurllatttsmraawtleserwerearateddmaaitdnamiisantrieesrtreeedproe3rd5t0e3,d540i0,n04,25, wD2e0u0rP,eo2na4td0m,Rien3pi0os0tr,etr3Ne5od0.,2242509,05,-3855100.,0,4Oo0lr0d,e7r2450m0a,ml/eokrer6a.t7s0wOmelgrd/eekragd.fmeimTnahilesetreartesd rseucrvoivveirnygpreartisowdearnedwtehiegnhseadcraifnidceodb.seTrhveedLdDusroinvgalaue1s4-wdearye calculated No from the mortality data. MAomrtmaolnitiyurmatipoesrfflouromroaolcetanneowabteo,rnpurraittsywaeprpero0x/i10m,at0e/l1y0,100/010%, 10/10, References og1cr0co7uu1pr0sr,,eadrneudspp1e10c0/ti16v0edlfayoy.rstaNhfeote1rc3l0di,onsi2ci0anl0g,,si2g4n0s,w2e8r0e, 3re3p0o,rtaend.d 3D7e0amtheg g a0M/no1dr0t,3a2l5i07t1my0,gr/a6tk/i1go0s,grfaoonrudpfse1,m0ar/le1se0penfceotrwivbteholery.n13r0Na,tos1wc6le0i,rnei2c00a0/l,1s02i,2g0n0,s/1204,00,/1208,0, reported. Deaths occurred up to 4 days after dosing, were g7Mr/oo1r0ut,pasla,intrdyes8rp/aet1ci0toisfvoferolryt.hmeaS3lt5ea0iw,nee4ad0n0la,in4nd5gw0r,eit5s2p5we,reir6ne7e0a0l,/1aa0rn,eda2,7/w110e0,akm2n/a10g, 3110, weight dosing. loss were observed. Deaths occurred up to 3 days ess, after and SMt1oa1ri0anlfeiodrtaytnhrdeatw3ie5o0ts,pf4oe0rr0ifn,ee4ma5al0la,ereawane,dasnt6la7ii0nngemdrgaf/takscgew,egwrreeoaukp1n/s1e,0s,rse,3s/p1ae0nc,tdi3vw/ee1li0yg,.hatnd oss were observed. Deaths occurred up to 3 days afer dosing Mortality ratio DuPont Report data No. for the 295-81 young adult males are covered in aMnordta1l0i/t1y0rfaotriotshefo3r50f,em4a2l5e,y5o00u,nganaddu6lt70ramtsg/wkegregr1o/u1p0,s,2/10, 8/10, driesscphecatrigvee,lyd.iarSrthaeian,eadnadndwewiegthtpelroisnscwaleraereoab,ssetraviende.d fDaecaet,hsn.asal occurred up to 4 days after dosing. 7M1o20r701t0am,lgi/1tk0y/gr1a0gt,riooausnpdfso,r1r0te/hs1ep0eocfltodirevretlhmyea.l20eS0tr,aait2sn4e0wd,efr3a0ec0e0,,/1s30t5,a0i,0n/4e10d06,a,n05/d0100w,,eta9/n1d0, 9pdiedarrairnyheseaaal,ftaaerrneadd,owsweieinaggk.hntesloss,stwreemroerso,bsleertvheadr.gy,Decahtrhosmoodcaccurryroerdthuepat,o rM9e/os1rp0te,acltaiinvtdeyly1r.a0t/i1oS0staffioonrrettdhheefa2ocl2ed5,e,srt3a5fi0en,mea4dl0ea0n,rda4tws5e0w,terapeenrdi1n6/e17a00l, ma5r/gc1/a0k,,g6/g1r0o,ups, wDeeaaktnhessosc,cutrrremeodrsu,p lteot5hadrgayy,saanftderwediosgihntg,loss were observed. DuPont Co. No. 788-82. (1982). Unpublished Data, Haskell Laboratory Report DuPont Co. No. 295.81 (1981). Unpublished Data, Haskell Laboratory Report jE} | 3 Type: Species/strain: Value: Oral Male aLnDdsofeRmaanlgeerfaitnsd/eCrrla:nCdDLiver Weight Comparison Test 5M5a4lemgr/atksg;,43sl9opmeg/6.k1g) (confidence limits, lower 294 mg/kg, upper 2Ca7s6trmagt/ekdg,maulpeperarts6:1499m1g/mkgg/,kgsl(ocpoen6f.i7d)ence limits, lower Smale rats; 459 607 mg/kg, slope mg/kg 5.2) (confidence limits, lower 315 mg/kg, upper Method: 2O5ve9rmigc/cktgo,miuzpepderfe4m9a4lemgra/tksg:,40sl0ompeg/7.k2g) (confidence limits, lower tcIononmaonroiLmlDa,lswomaasrlaanedgmreaiftnisi,nsdcetaresrtteredatt,beydthmienattleresatgrasastutsbr,sitcnaoinrncmteau,blaatsfieaomnasluienspsreiantnssg,ileondoinses (6o17v0a0rrmiaegtsc//tkgogrmoiwuzpee)r.defteemsatlede irnatcs.acDhoosfethleevteelsstosfys2t0e0,ms48m0e,ntainodnedaanbdove GLP: ReRseuslttsS:ubstance: MtIhnaeltetheesgtlriosvuuebprsswteawniecgrehe,tdacesofmaipnsaeudrsipasesonmnsaitloenes,icno5nctrroaotmls,/ogimlra,oluaeps wa1e0ir0nemggla/demkdigon,sies,tered F2e0m0amlge/kger,oumpaslweecraestdreaftiendecdonatsrfole,maalnedcmoantlreolc,asftermaatlede 210000 mmegmkasl,,e dofuveramiranilgeeca2to01m04i-mzdgea/dyk2rg0e,c0ofmvegem/rakylgep.eorviAaolrdli.rectTtshoemwierzraetesdwwceoeinrgierhoetlhd,eaannnsddacforbeismfieacrleveded the No livers were weighed. and aIAnnmdtmh8eo/n1L0Disuaotmssptiuendrytf,hleumoo2rr0ot0oa,cl4ai5tn0yo,aitnaen,tdhpeu6mr7ai0tlyemagrp/aptkrsgooxgcircmouaurtprese,ldyrien1sp00e/0c1t%0i,ve7l/10, SM/o1rt0aaltitsyinintthhee2c0a0s,tr4a8t0e,damnadle67r0atsmgo/cckugrrgeroduipns,0/r1e0s,pe5c/i1i0v,elayn.d y. otMvhoearrt2ai0le0ic,tty4o8mo0if,zteahdnefdfee6mm7aa0lleemrgra/attsksgoocgccrcuoururrpresed,d irinens0p0/e/1c10i0,i,v8e71l/y01.0,, aManondrdt9a7/l/1i10t0y riantsthien 2200, 480, and 670 mg/kg, respectively. mg/kg aInndth2e0l0ivmegr/wkegi,ghnto weight alos were ccohmapnagreissionnlsitveurdywetiegsthetd oart idnolsievelrev0elsboodfy0, seen n the female rats given single 100, i10t.h2e00 1m0g0/okrg 2(0o0vamrgi/ekctgopmirzoedducoerdnoarnmailn)c.reaAsesiinngilveeorrawdleoidsgoehgsteooofffup Reference: imnaclreeacsaet,s.buCtasattrsatciaosntrraetdeducaenddtghievmeang2n0i0tumdge/kofgthhaedlihveearvwieeirglhitvers ptehrafnludoirdotohcetacnoonattreol-st.reaCtleidnircaatlssiingcnlsudsecdenstianitnheed afamcmeoannidupmerineal tahreeae,xapnerdismpeonrta.dic weight loss. No deaths occurred in this phase of NDou.Po6n0t0-C8o1.. (1981). Unpublished Data, Haskell Laboratory Report Type: MeStpehcoieds:/strain: GLP: Test Substance: Results: Acute Oral Toxicity Mal `The e t ra est ts/Crl:CD substance, ammonium perfluorooctanoate (3M) an d oaiml,mwoenrieuamdmpienrifsltueorreodocbtyanionattraeg(asRtirmicarinCtou.b)a,tiasonsiunspseinngslieondsosiensctoom 4`8y0o,unogra6du7l0t mmga/lkegroaftst.heRattesst(s1u0b/sgtraonucpe). wTerhee asdumrivniivsitnegrreadts2w00e,re weighed and sacrificed. observed during a 14-day recovery period and then No Ammonium perfluorooctanoate (3M), purity approximately 100% MAomrtmaolnitiyuamtipoesrfolfu0o/r1o0o,ct6a/n1o0a,taen(dRi1m0a/1r0Cwo.e)r,epfuroiutnyd9i5n %themi20n0i,mum ir4ne8s0tp,heecat2ni0dv0e6,l7y4.080mM,ogra/tnkadgli6at7my0mrmaotgni/oiksugomfap0me/mr1f0o,lnu1io0ru/om1o0cp,teaarnnfodlaut1oer0o/(o13c0Mt)waengorraeotuefposu,nd (Rimar Co.) groups, respectively. Reference: iCnlcinliucdaeldssitganisneodbsaenrdv/eodr winetampemroinneiaulmarpeae,rfsltuaoirnoeodcftaacneo,aatend(3wMei)grhatts Toss at all levels tested. Other clinical signs observed included (w2e0a0knmegs/skg()4.80Alalndde6a7t0hsmogc/ckugr)r,eddiwairtrhhiena 4(4d8a0ymsga/fkige)r,doasnidnagl.opecia CColi.n)icraatlssiingcnlsuodbedsesrtvaeidneidn aanmd/moornwieutmpepreirnfelauloraoreoac,tasntoaaitneed(fRaicmea,r ianncdluwdeeidghwtealoksnseastsa(ll48le0vaelnsdt6es7t0edm.g/Oktgh)erancldinliectahlaSriggyn(s6n7o0temdg/kg). All deaths occurred DuPont Co. (1981). within 4 days Unpublished after dosing. Data, Haskell Laboratory Report No. 565-81 Type: Species/suain: Value: Method: Oral Male AraLtDs/(ChARp-pCrDoximate Lethal Dose) 670 mg/kg The test substance, as an aqueous solution (pH ~6), was GLP: ReTseuslttSsu:bstance: {a1dmrianti/sgtroeurpe)dibnysiinntgrlaegadsotsreisc.inCtounbcaetnitornattoioynosuntegstaeddulwtemraelIe, r1a.t5s, 2.3, 232.45,05m.g1/,k2g6.,40S,ur6v0i,v7o7r,s 9w0e,re12s0a,cr1i3f0i,ce1d701,42d0a0y,s3l0a0t,er,45a0n,d6b7o0,dyand N`woeights and liver weights were recorded. DAemamtohsnidiudmnopterofclcuuorroaotcdtoasnoealteev,elpsuroifty1n-o4t50spmegc/ikfgie.d The rat dosed with 2250 670 mg/kg died 2 mg/kg died | day days after adfotseirndgo.sing, and the rat dosed with Reference: S1l-i2gdhatyisn.itiAalt9we0igmhgt/klogssaensdoacbcouvrer,edthaet f2e6cemsgw/ekrgeasnmdalalboavnedfor luiinrvrceeorgmeufnloalrratlraygb-eslmheeanfpoterdnf1-or5ratd1sa-6wyisdtaahyftsseiranfgdtloeesriddnoogss.eisnTgah,sealncodowmtaphseo6ur0ant-ds9w0ceamruges/ekdg. iBAndacdslieutddieoodnnaclchlteionwxiiicncalgssimigogntnsiso,atnist,haelpsololeytauhcartlieadd,oaissnecsar(egaa6ss7te0rdoairnnedtsep2sit2ria5nt0ailomnigrr/iakttagen,)ta.nd f2a2c5e0-mpagw/iknggaonndtshleigdhatysaolfidvoastiinogn.aftSehrodvoesli-nngosoicncgurwraesd oabtserved at 670 mg/kg. DuPont Co. No. 128-68. (1968). Unpublished Data, Haskell Laboratory Report Type: Species/strain: Value: Method: GLP: Test Substance: Results: Oral ALD M6a7l0emgr/aklgs/ChR-CD `mTahleetersattssu(1bsrtaagncreouwpa)s iandsmiinngilsetedroseeds.by Cionntcraegnatsrtartiicoinnstutbeasttieodnwetroe: S1u5r,vi1v2o,r4s0,we1r2e0,sa2c0r0i,fi3c0e0d,1445-01,56d7a0y,s1l0a0te0r,, a15n0d0,boadnyd w2e2i5g0htmsg/akngd. liver No weights were recorded. MAomrtmaolnitiyuomccpuerrrfelduoarto6o7ct0anmoga/tke,gpaunrditayb9ov9e%. Clinical signs of dtioxsicchiatrygienacrlouudendd itnhaectniovsiety((61720,0,11500000,,an1d5020,25a0ndmg2/2k5g0),mpge/rkign)e,alred 44d5i50s0c,om6lg7o/0rk,agt)ai,onndi(m1i3t00a00b0ialmnigtd/yk4(g56)0.70mgAm/gnk/igkm)ga,)l,rsuafsnfadlcerwdieffiiucgrehdt(31l04o0s-s1a5n(4dd0a,ys20a0f,ie3r00, ehnalvairnggedrelcievievresdwdhoesnescodmopwanre10d atnodcoinntcrlouldirnatgs2w0e0igmhgi/nkggbheatdween 5li0v0er-5w5e0igghtw/ibthodlyivweeriwgehitghptesrcoefnt2a2g.e6,s 2o0.f7,4.21,93..18,,a3n.6d,2a1n.d7 g3.8a,nd respectively. Liver weights at 200, 300, and 450 mg/kg were 30.6, Reference 52.53.,2,anadnd6.228,.r2esg,pewcittivhelliyv.erLwieviegrhetn/lbaordgyemweenitghwtapseralcseontpaogsessibolefin5.6, wraotuslddosheadvebetolobwe 2c0o0mpmaer/ekde,inbuotrdaedrdittoieosntaalbtleissthatnhids.control rats DuPont Co. No. 5561. (1961). Unpublished Data, Haskell Laboratory Report Type: MSeptehcoieds:/strain: GLP: Test Substance: Results: Reference: AtocxuitcietyOroaflaEmfmfeocntsi,uDmoepserpfrleutorreoaotcmteanntoawtiet?h ethanol modify the MpYaeorlufelnrugcartaodsou/clCttramlna:olCaDteeraatssa(66/0g%rouapq)uewoeursesaodlmuitniiosntaetrdedosaemlmeovenlis uofm200, w48i0t,h eatnhdan6o7l0(m1g5/k%g.v/vA)didnitdiroinnaklinggrwoautpesrofforrat1s4wdearyesparned-ttrheeanted cdoonsceedntwriatthioanmsmaosnliisutemdpaebrofvleuoornoodcatyan1o5a.teTahtrteheeostahemregroups of irnattsrawgaesrteripcrienttreuabtaetdiownitfholalosiwnegdle2460h0ou0rmsgl/atkegr wdiotsheotfheetahmamnoolniviuam apdedriftliuoonraolocgtraonuopatoefturnetartemaetnetd aratttshseelrevveedlsaslicsotnetdraoblosv.e.GrAounps of r1a5ts%wveirveianldsroitnrkeiantgedwwaittehr feotrha1n4oldaaylso.neCaltineiictahlersi6g0n0s0amndg/bkogdyo.r swaecirgifhitcse.were recorded. Liver weights were recorded at the 14-day No AAlmlmroatnsidiuemd pweirtfhliunor8odocatyasnaofatteer,dpousriintgy waiptphroaxmimmaotneilyum100% spehrofwleudoraonocitnacnroeaatseeaitn6a7ll0rmatgs/ktgh.at rLeicveeirvewdeiagmhmtobnoidyumweight ratios tpheerfalnuiomraoloscttahnaotarteecewihveend ocnolmypaetrheadnolt.o thTeheurnterweaetreedncoonstirgonlisfiacnadnt rdeicfefeirveendceosnlbyeettwheaenonl.theThunetrreeawteerdeconnotrsoilgsniafincdantthediafnfiemraelncsetshat pbeertfwleueonroroacttsatnhoaattreeacnedivtehdeertahtasntohlatanrdecaeimvmeod noinluymammonium perfluorooctanoate. NDou.Po7n9-t84C.o. (1984). Unpublished Data, Haskell Laboratory Report Type: Species/strain: Value: Method: Oral Male LanDdsyfemale mice/CD-1 3M4a4lemga/nkdg,feumpapleerm5ic6e0: 457 mg/kg (95% mg/ke, slope 6.6) confidence limits, lower iTnhteragteassttrsiucbsitnatnucbea,ti4osn a suspension in in single doses tcoo6mgoriol,upwsasofamdamlineiastnedred by 6 groupsoffemale young adult mice. Ten male and female GLP: Test Substance: Results: 4m0ic0e0/mggro/ukpgowferteheadtmesitnissutbestraendc2e5.0,Th5e00s,u7r5v0i,vin1g00r0a,ts20w0e0r,e owreighed Tahnde oLbDsesrvveadludeusrwinegreac1a4l-cdualaytreedcforvoermytpheermioordtaalnidtythdeatna.sacTrhifeicmead.le and the LfDesmyaldeat4a00du0eatnodl2im0i0t0atmiogn/skogftdhoseecloemvpeultsewre.re not calculated in No fAMoomrumtnadolniintiytuhrmeat2ipo5es0r,folf5u0o10r/,o1o07c,5t40a/,n1o01a,0t0e10,0,/p1u20r0,i0t01y,0a/a1pn0pd,ro41x00i/0m10a0,tmeagln/ydkg1100m0/a1%l0eweanrde female groups, respectively. Clinical perineal signs area, woebaskenrevsesd,inanmdalweeimgihcteloisnscliundaeldl sstuarivnievdinagndm/iocre.wet Orutfhfelredsifgunrs(2i5n0claundded5e0y0esmhga/lkfg)c,loasteadxi(a2(52050a,nd5001,00a0ndmg/kg), m1i0c0e0omcgc/ukrgr)e,dawnidthtirnem6ordsay(s50af0tearnddos7i5n0g.mg/kg). All deaths in male Reference: pCelriinniecaall sariegan,s woebaskenrevsesd,inanfdemwaelieghmticloessinicnlualdledsusrtvaiivniendgamnidc/e.or wet (O7t5h0ermsgi/gkngs),intcrleumdoerdse(y7e5s0hmaglf/kcgl)o,seadta(x7i5a0(m5g0/0kmgg)/,ksgt)a,inaenddfraucfefled fur (250 within 7 adnayds7a5f0temrgd/oksgi)n.g. All deaths in female mice occurred DuPont Co. No. 329-81. (1981). Unpublished Data, Haskell Laboratory Report Type: Species/strain: Value: Oral LDsy Male Male agunidnfeeampailges:gu1i7n8eamgp/ikggs/(D9u5n%cacnoHnafritdleenyce limits, lower 144 mg/kg, upper 202 mg/kg, slope 8.9) Method: GLP: Test Substance: Results: F1e8m7amlge/kggu,inuepappeirgs2:4621m7g/mkgg/,kgsl(op9e5%8.6c)onfidence limits, lower Tinhteragteassttrsiucbsitnatnucbea,tiaosn ainssuisnpgelnesidoonseisn 1c0o6mgoriol,upwsasofamdamilneisatnedred by 6gugirnoeuappsiogfs/fgermoaulpewyeoruenagdmaidunlitstgeuriendea15p0i,gs.200T,e2n50m,al3e00a,n4d00f,emoarle 6we7i0gmheedkandofotbhseertevsetdsudbusrtianngcea.14T-hdeaysurrevciovvienrgygpueirnieoadpaingds twheerne sacrificed. data. The LDsp values were calculated from the mortality No AMomrmtaolnitiyurmatipoesrofflu3o/r1o0o,ct6a/n1o0a,te9,/1p0u,ri1t0y/1a0p,pr1o0x/i10m,ataenldy 1100/010%were 7 18 Reference: Type: Species/strain: Method: GLP: Test Substance: Results: rfeosupnedctiinvetlhye. 1M5o0r,t2a0l0i,ty25ra0t,io3s00o,f420/010,,a1n/d106,780/1m0g,/9k/g10m,al10e/1g0r,ouapnsd,. f1e0m/a1l0ewgerroeupfso,unrdesipnectthieve1l5y0., 200, 250, 300, 400, and 670 mg/kg Cwleitnipcearlinseiaglnsaroebasaenrvdewdeiankmnaelses gatuialnlealepvieglss itensctledu.dedOtshteairnceldinaincda/lor s2i0g0n,saonbds6er7v0emdgi/nkgth)e, meaylesehgaulifncelaospeidgs(i2n0c0luadnedd3s0ta0imnegd/kfga)c,e (150, (l1ac5r0immgat/ikogn),(1a5n0dahnudnc2h0e0dmpgo/sktgu)r,ea(t2a0xi0am(g6/7k0g)m.g/Wkegi),ghttrelmoosrss ooccccuurrrreedd awtitahllinle4vedlasytsesatfetedredxocseipntg.400 and 670 mg/kg. All deaths Calnidn/iocralwestigpnesrionbesaelrvareedaiannfdemwaelaekngeusisneaat palilgsleivnecllsutdeesdtesdtaeixnceedpt p4i0g0s ainndcl6u7de0dmcgo/nkvg.ulsOitohnser(c6l7i0nimcgal/ksgi)g,nsatnaoxtiead (i6n7f0emmga/lkegg),uinca tgraesmpoirnsg((460700 mmgg//kkgg)),, leaycersihmaaltfiocnlo(s2e5d0(m1g5/0kga)n,ds2t5ai0nmegd/fkagc)e, l(e2v0e0lsmtge/sktge)d,eaxcnedpltetfhorar6g7y0(m1g5/0kmgg./kAgl)l. 6 days after dosing. dWeaetihgshotclcousrsreodccwuirtrheidn at all DuPont Co. No. 291-81 (1981). Unpublished Data, Haskell Laboratory Report MAacluetedoTgosx/ibceiatgyle sDuobgstsan(c2e/.gro`uTph)e rfeocleliovweidngeibtihoecrh2e0m0icoarl4m5e0asmugr/ekmgenoftsthweetreestmade on the blood: phosphatase. sugar, When utrheea4n5i0trmogg/enk,gtdotoaslecwhoalsesatdemrionl,isatnedreadl,katlhiene dleevheyldofroacgteinvaisteyo(IfCDlaHc)ti,c adledholyadsreo,ggelnuatsaemi(cLDoHxa)l,aciestociictrtircansaminase m(eGaOsTur)eda.nd Aglruotuatmiincephyermuavtioclotgriacnaslameixnaamsien(atGiPoTn)awnedreanalasnoalysis of Tah2e4-lhevoeulr oufrivnaerisopuescciommepnownaesntmsadofe tahte ibnltoerovdalfsololnotwhiensgedaonsiimnaglsw. as cwiotmhpaarseidmiwliatrhmeanasauvreermaegnetvamlaudeeobast etrhveesdapmreiotritmoe eoxnposspuerceimaennds wfarsomessttaobclkicsoheldonfyrodomgsm.easFuorretmheenetnszmyamdeeacotnivsittioecsk, daognso.rmTalherange activity No was also measured at least once prior to treatment AThmemGonPiTuamndpeGrOflTuorleovoecltsanwoeartee,elpeuvraittyed9w9i%thin 48 hours in both Reference: ndoorgmsatlhartanrgeec.eivWehde2n0045m0g/mkgg./kgOnweaswaedemkinliasttee,retdh,eyalwleorfethien the tTehnhazetyrgmereceaestievmseetdacs4hu5ar0negdmegw/oekcrcgeudrmiraeerddkweiindtlthyhienelG4eP8vTahtoeaudnrds24IaCfteoDrH4.8dohsBoiountgr,hs laanteirm.als NDou.Po1n2t3-6C5o.. (1965). Unpublished Data, Haskell Laboratory Report Type: Species/strain: IRnahtasl/aCthiRo-nCDA.LC (Acute Lethal Concentration) VEaxlpuoes:ure Time: 4 hours 08 mg/L Method: eMxaploeseradtsto(6du/setxspoosfuraemlmeovenl)i,uwmeipegrhfilnugor2o5o0c-t2an7o0agteraamtsexwpeorseure `hlweehvaeodl-lsoen-olbyfo,dye0.x3e8cx,ep0po.st8u1fr,oer0..t8hT3e,w22o..22d,mif4g.f/8e,rLeonertxp5m.oe7stumhrgoe/dLsloe.vfeEldxuwpshotesrgueerneresartawstehiroaend twheerfeirusstemdettohogde,naerahteeavaymmcloonuiduwmaspegrefnleuroartoeodctbaynobaltoewaienrgosols. Tn tCaeups-pttrsaoubxbiisntmagantjceeetl.ysu3Tb0hmeLeiramigirencduatricrniaoedfmaetihcrehtadhnurisoctuapglahlrytaihscitliegrshreidvnetroleosacenirtvyoiLr of the tehxepogsenuerreacthoarmabnedr.theSucihtaabmlbeedriltoutaicnhgiaeivrewlaosweirntcroondcuecnetdrabtieotnwse,en slTaehrcegoengdeannmedertasmthaoolrdl,d,iawdegneronetedrfearltaiocvtreiwroenaadsteiuntstheoedtdhfueosret,xlpoaownsduartalemlocpshaprahtmeibrcelierc.sdiuzIsents,the cfcaeorsnrecireevndotirtrahteiimoppnairst.nigceAldesfoatnloliaangcpynscetluromenaaetmihocefjaeddtu.swthTephareretaitichrleessltarfrergaoemrmafosrntoeimsrrwteheidrsejet asrinezdteudfmuresadtctctiooonntachteeinotrner.saetrTivohoniers.gweenTreheriasltogowerenweraresadtrbourynaducilhnuidteeivrnegcdotnshseotmsaetntrpecaaromtnidwciiltetihons caoirncbeenttwreateinotnhsewgeerneerdaettoerramnidnetdhe5etxipmoessudruercihnagmebaecrh. 4-Thhoeurairborne deixsptorsiubruet.ionAmtetahseumriedm-epnotinwtoasf each made. exposure, a particle size GLP: Test Substance: Results: wCliitnhicfalluosriogsncseiwneriemmreedcioartdeedl.y aTfhteereeyxepsosoufret.he Rraattsswweerreesstaacirniefdiced pfoorsth-iesxtpoopsautrheo.logTiicsseuxeasmifnraotmitownoatra1t,s7d,y1i4n,g2d7u,roirng42exdpaoyssure were also No examined microscopically. TAhmemmoonritaulmityperraftliuoosrfooorcttaheno0a.t3e8,, purity 0.81, 0a.p8p3,ro2x.i2m,a4t.e8,lyan1d050.%7 mg/L. 10 10 References: Type: Species/strain: Exposure Time: Value: Method: e6/x6p,oasnudre6/g6r,ourepsspewcetirveelLy. (Anlolt dteesattshusbsotcacnucrer.erdelcaittheedr),d2u/r6i,ng1/6, 6/6, exposure or within 48 hours after exposure. eCxlipnoiscuarlesilegvneslso)b,sierrrveegduldarurbirnegatehxipnogsu(arlel eixnpclousduerdeglaevseplisn)g, (raeldl dsailsicvhaatrigoen (a2r2o,un4d.8t,heanedye5s.7anmdg/nLo)s.e (Callilniecxaplossuigrnesloebveslesr)v,eadnd p(o2s2t-nedxp4o.s8urmegi/nLc)l,udaendd s>o8m0e%rurpattusrheaovfinegyeos,paaqlluoetahnerdeyes opaque cofortrhoedeeyde-sapwpaesarcionngfeiyremsed(0w.i8t1hafnlduo0r.e8s3cemign/Ls)t.ain E(x0.t8e1m,a0l.8d3a,maangde (20..281mgm/gL/)L,).with the eyes appearing normal after 20 days LrIienvhaeaclrhaewtdieoiangmhotafsxtriheemtutuemsmte(sd2utbtositmtaehnsecnneoocrrammuaaslle)dr7al-ni1gv4eerd4ea2nyldsaaaryfsgteeamrfetenexrtpoewsxhupiroecs.hure. eNxoamcihnaantgieosniinnldiivceartecdeltlhmatorapcuhtoelopguylmwoenraeryobesderevmead.deMvieclroopsecdopic prersoimdputallyi,njbuurty.diNsaoppdeuasrtepdaritniaclpepsrowxeirmeasteeelny i1nwteheekl,ulnegasving no 2miwceroeskcso.piAcamlmlyo.niTuhemrepewrafsluiorrroiotcattiaonnoaotfethaelssotcoamuascehdtchoartncelaelared in opacity 42 days aanftderulecxeproastuiroen.that were still microscopically evident DNou.Po1n6t0-C69o.. (1969). Unpublished Data, Haskell Laboratory Report Kennedy, G. L., Jr. etal. (1983). The Toxicologist, 3:22. Kennedy, G. L., 24:1325-1329. Jr. etal. (1986). Food Chem, Toxicol, MDaelremraaltsL/CDhR-CD 24 hours R6a9t5s9wmegr/ekcgli(p9p5e%d cfroenefiodfehnacier olivmeirtsthuendbeafcikneadn)d trunk area. Five 7500 mmagl/ekrgatasm/mdoosnc ileuvmelpewrefrleuodroosoectdanwoiatthe.300T0h,e5t0e0s0t,suabnsdtance, as und50e/r5a0 asqquuaeroeusofsauslpuemnisniounm,fwoailsaanpdplhieeldd tion tphleacbeacwkitohfcelaacsthicrat sbapnodnaggeeds.offAfwtietrh2a4mhioludrdseotefregxepnot,surrien,setdh,edrraitesd,wearned urnewtruampepdetdo, their cages for 13 days observation or until death. During the GLP: Test Substance: Results: References: osibgsnesr.vaGtrioosnspepraitohdo,ltohgeyrwaatsswdeorneewoenigalhlesduravnidvoorbssearnvded1 afnorimcallinitchaalt walassefxocuenpdtd1e.aTdhaet 7L50D0 mvgal/ukeg.waLsivcearlcwueliagthedtsfrwoemrethreecmoorrdtaeldiitnyall data. No MAomrtmaolnitiyurmatipoesrfwleuroero0o/c5t,a0n/o5a,tea,npdu4r/it5yatap3p0r0o0x,im5a0t0e0l,yan1d00% A7l5l00ramsg/dkogs,edreatsp3e0c0ti0v,el5y0.00D,eaantdh 7oc5c0u0rrmegd/kwigthhiand 2inditaiyalsowfediogshitng. 7lo5s0s.0 mCgli/nkigc)a,l wseigtnpseoribnseearlveadreian(c5lu0d0e0dalnedth7a0r0g0y (m5g0/0k0g)a,nsdtained cpherrionmeoadlaacrreyao(r7r5h0e0a m(g7/5k0g0),mgs/tkagi)n.edGnroosses(p7a5t0h0olmogg/ykgs)h,oawnedd an increase in liver posttreatment. weights in all surviving rats examined at 14 days DNou.Po6n5t9-C7o9.. (1979). Unpublished Data, Haskell Laboratory Report Type: Species/strain: EVaxlpuoes:ure Time: Method: GLP. Test Substance: Results: References: 8K1e:n3n4e8d-y3,55G.. L., Jr. (1985). ToxApipl.cPhoarmlaco.l, FDeemramlaelraLtDss/yChR-CD 24 hours R>a7t5s0w0ermeg/ckligpp(etdhefrmeeaxofimhauimr feasible over the dose) back and trunk area. aFimvmeofneimualme preartfsl/udoorsoeocletvaenloawteer.e Tdhoesetdeswtistuhb5st0a0n0coe,ra7s5a0050m/g5/0kg asqquuaeroeusofsuaslpuemnisinounm,fwoialsaanpdplhieeldd tion tphleacbeacwkiothfecalacshticrabtaundnadgeesar. `wAiftthera2m4ilhdoudresteorfgeenxtp,osruirnsee,d,thderireadt,s waenrdereutnuwrrnaepdpteodt,hesiprocnaggeedsoffofr t1h3e draayiss woebrseerwveatiigohnedoraunndtiolbdseeartvhe.d Dfuorricnlignitchaelosbisgnesr.vatSiuornvipveirniogd, rats were sacrificed. No `ATmhemmoonrtiaulmitypewrafslu0o/r5ooacntdan1o/t5ea,t p5u0r0i0tyaanpdpr7o5x0i0mamtge/lkyg,100% preesrpfelcutoirvoelayc.tanDoeaattehcoacucsuerdrmeidl3d dsakyins airfrtietrattiroenatamnedntw.eiAghmtmolonssiuatm pbeortihnedaolsearleeavewles.reIonbasdedritvieodn,ats7ta5i0n0edmgfa/ckegand wet and stained DuPont Co. (1980). No. 682-80. Unpublished Data, Haskell Laboratory Report Kennedy, G. 81:348-355. L. Jr. (1985). Toxicol. Appl. Pharmacol., Type: Species/strain: Dermal LDgy Male rabbits/New Zealand White VEaxlpuoes:ure Time: 24 hours 4278 meg (95% confidence limits, lower 2369 mg/kg, upper Method: wR9ae8br1be4itfmisgtwt/eekdrgw,eicstllhioppppleaesd6t.i2cf5rce0oe4l)loafrsh.airFiovvee,r5t,h5e,baancdk2anmdalterurnakbbairtesa,wearned pdeorsfelduowriotohct1a5n0o0a,te3,00re0s,pe5c0t0i0v,elayn.dT7h5e00tesmtgs/ukbgstaamncmeownaisummade into taesstlusrirtey wwaitshowcactleurd,ewda(swarpapplpieedd wtiotthheplbaastcikcowfreaapc,hgaruabzbei,t,anadnd the fuelonarwsrt1ia4cpopbreand1d,5agwdeaasys)sh. eoAdbfstweiretvrha2tw4iaotnheorou,rrdsurnoitfeidle,xdpeaoantsdhu.rreetD,uurrtnhieendgatntoihmteahelisr wceargees colbisneircvalatsiiognnsp.erGirood,ssthpeatahniomlaolgsy wwearsedwoeniegohnedallansdurovbisveorrvseadndfor cLalacnuilmaatledthfartowmatshefomuonrdtadleitayddaatta3.000 mg/kg. The LDso value was GLP: No ReTseuslttSsu:bstance: MAomrtmaolnitiyurmatipoesroffluroarboboicttsanwoeartee,0/p5u,ri1t7y5,ap3/p5r,oxainmda2t/e2lyat110500%0, 3000, 5000. and of dosing. 7G5r0o0ssmgp/atkhgo,lorgesipceacltievxealmyi.naDteiaotnhsohcocwurerdednowithin 4 days dcoosmepoluenvdel-sr.elCaltineidcaclhasniggenss;ihncolwuedveedr,wetihgehret was loss skin and liarbiotrateidon at all nbarseaalthdiinsgchaatraglled(oSs0e00lemvegl/sk.g)I,nsahdadliltioown,brleeatthhairnggy ((53000000 mmgg//kkgg)),, (pa5l0l0o0r m(g5/0k0g0),mgw/ektg)u,nddieamretahetah(tSh0e0b0omdgy/k(g5)0,00weaankdn7e5s0s0 mg/ke), References: and cyanosis (7500 DuPont Co. (1979). mg/kg) were observed. Unpublished Data, Haskell Laboratory Report No. 639-79. Kennedy, G. 81:348-355 L., Jr. (1985). Toxicol. Appl. Pharmacol,, Type: MSeptehcoieds:/strain: Dermal Irritation SMiaxlemarlaebbriatbbsi/tNsewweZreealcalnipdpWedhiftreee of hair on the trunk and lateral aarmemaso,nainudmplpaecrefdluionroFoDcAt-atnoyapteeswteorckes.appDloiseedsasofan0.5aqguesooluisdpaste to the intact skin semi-occluded under gauze squares, (rubber sheeting was and the loosely test site was wrapped around the GLP: ReTseustltSsu:bstance: Reference: wterurnekraenmdosveecdurferdomwitthheasdthoeckssi,vethteappea)t.chAefsierrem2o4vheodu,rsa,ndthethreabbits reactions No observed. Observations were also made at 48 hours. AAmmmmoonniiuumm ppeerrfflluuoortooooccttaannooaattee,cpauursietdy mapiplrdotxoimmaotdeelryat1e0s0k%in wimhietantitoenstiend 24 on htohuerss,haavneddsilnitgahctttsokminodoefrraatbebiitrsriutantdieorn in 48 hours DsuemPio-notccClou.de(d19c7o9n)d.itiUonnpsu.blished No. 636-79. Data, Haskell Laboratory Report Type: Species/strain: Method: GLP: Test Substance: MDaelremarlabIbrirtist/aNteiownZealand White `aTnwdetnrtuyn-konaeremasa,leanrdabfbiitttsedwweirtehcplliapspteicd cforlelearos.f haAimrmoovenrituhme back perfluorooctanoate bpaercfklouofreoaocchtarnaobbaitte. impregnated Teflon" imTphrreegenaratbebditKsesveiravred strips or ammonium satsricposnwtreorles aapnpdliweedrteo the Twhreaptpreudnkionfneoacnh-armambbointiwuamsptehrefnluoocrcolocutdaendoa(twer-atprpeaetdewdimtahtearilaaly.er Aofftpelras4t-i,c8w-,raopr,2g4a-uhzoeursterxeptocshubraendpaegrieo,dsa,ndthaedwhresaipvpeinsgtrsewtcehrteape). wreermeovseodakaendditnhae 5e0x:p5o0seetdhaanroelas:wwaetreerwsiolpuetdiowni.thThgeauazneimpaaldss wthearte oNboserved after unwrapping for skin irritation f0Ai.mb0emr6so1,n%3ia8um%mmTopenefrilfuolnmuorproeeorscfitlnautonhroaoatotcietniacmnlopuardteeegd,n2aa.tne9dd%1TT4erfi%ltdooinnmeX(th-4y18l0%s0iTlaiencfdolnoen oil) Results: Reference: Type: Species/strain: Method: XKA-em1vm0io0arannifduibm0er.ps0e,6r4f72l%5u%oarmKoeomcvotlnaanirouamtreespiienmrfptlrhuaeotgrnioanotcceltduandKoeaedtv3el,.a2ra%nd(T4r10i7t..o55n%% dimethylsilicone No skin irritation oil) was observed during the study. NDou.Po7n3t6-C8o1.. (1981). Unpublished Data, Haskell Laboratory Report ERaybebiIrtrsi/tNaetwioZnealand White rOingeh-ttceonntjhunmcLtiv(a3l8.s3acmgof) eoafcshoolfid2tersatbbsiutbsst(asnexcenowtassppecliafcieedd)i.ntoAfttheer T2h0esetcreoantdesd, e1yetroefattehdeeoytehewrarsabwbaisthweadswniotth wtaasphewda.terOfbosrer|vmaitniuotnes. GLP: Test Substance: Results: Reference: lofamtpheatco1maenad, 4irhso,uarsn,d acnondjautnc1t,i2v,a3w,7e,re14m,a2d1e, waintdh2a8hdaanyds-.slit aFfltueorrt-hLestdraiyp"osfttarienatamnednta, biomicroscope were used at examinations No AAmmmmoonniiuumm ppeerrfflluuoorrooooccttaannooaattee,cpauursietyd g1e0n0er%alized moderate emcyooedmeetrahaaltteowpacasictiust,nywawanisdthhmeoaddseamfrtaaeltretarcreoeanatjoumfnecnsttei.vveiTtrhieseowpoahccueilntayrt,eeisfntfetedecrtimsnitaternatbbit gradually persisted, arencdedated2;1-h2o8wedvaeyrs,wtahse small mild area with of comeal opacity vascularization (sign of wheaaslhinegd).haAdnaesymealdloasreedawoifthslitghhettteostmsoudbesrtaatnececoamnedaplroomppactiltyy and nmoordmearlatweittohisnig7hdtaycso,njeuxnccteipvtitfiosr wmiitlhd ncoonijiuntcctiefvfaelct.redTnehses,eywehiwcahs was normal within 14 days. NDou.Po6n35t-7C9o.. (1979). Unpublished Data, Haskell Laboratory Report Repeat Dose Toxicity Type: Species/Strain: Sex/Number: Exposure: Period: Frequency of Treatment: LeEvxpeolssu:re: Method: GLP: Test Substance: Results: ~~MRiecpee/aCtDe-d1Dose Oral Toxicity Study MRaatlse/aCnrdi:fCeDmale/S/group 9 doses (week) 3 weeks 0,0.1,10, 10 mgkg Tinhteragteassttrsiucbsitnatnucbea,tiaosn atoqureatosusansdolmuitcieo.ns,Cownatsroaldmriansisatnedremdicbey rreeccoeridveedd.waAtneirmaonllsy.weBroedsyacwreiifigchetds and clinical 3 days after signs were the final dose and the No livers were removed and weighed. AAmsmigonnifiiucamntpelirvfelruwoeroiogchttanionactree,aspeurwiatsy naoptperdoxiinmbaottehlyma1l0e0%and female mice liver weights at the were 1 and noted 10 in mg/kg levels. male rats, but oSnilgyniatfi1c0anmtgl/ykign.creTahseerde were no significant any dose. changes in the liver weights of female rats at Reference: oMcocrutralrietdyion cmuarlreemdiicne,2/m5alfeemraaltse, moircfeematal1e0rmagts/kagt.anNyodomsoertlaelvietly feestmeadl.e rSaptosraanddicinwtehieg0h.1,los1s.0,ocacnudrr1e0dming/tkheg0m.a1l,e1.a0n,danfdem1a0lemg/kg DmiucPeo.ntWeCao.kn(e1s98s3)w.asUonbpsuebrlviesdheidn Dthaeta1,0Hmagsk/eklglfLeambaolreatmoircye.Report No. 138-83. Type: Species/Strain: RReaptesa/tCreld:CDDoseOral Toxicity Study Sex/Number: ~~ Male/12 PeErxipoods:ure 12 days; 14 days of recovery Frequency of Treatment: Exposure 10daily doses (5 doses, 2 non-dose days, 5 doses) Levels: Method: 0,6.7 mg/kg Each o6f rats received 10 daily doses of ammonium spoelruftliuoonr.ooBcotadnyoawteeigbhytsinwtreargeasrterciocridnetdu.baTtihorneeasraatns awqeureeokuislled 414hdoauyrss alfatteerr. reOcregiavninwgetihgeht1s0wedroeser,eacnorddethde. rSeimxaiunnddeorsewderreatksilled GLP: served No as controls. ReTseuslttSsu:bstance: TAhmemmoonsitupmropmeirnfelnuotreofofcetcatnooaftteh,eptuersittsyu9bs9t%ance was enlargement of 4th5e%lihveera,viwehricthh,anatthtahteoefntdheofcotnhterdolosraagtse. rTehgiismecnh,anwgaespaebrosuitsted safotmerewcehsastatlieosns Soifndcoesa1g4ed,abyustltahteerwtehieghlitvedrisscwreerpeanocnylyw2as0% heavier than those of conirol rats. Reference: Tcohnetrroelnaaltsw,eiagnhdtsthiwseirnecr2e0a%se hpeearvsiiesrtetdhaafntetrhocseesosafticoonrroefsdpoosnadgien,g wbeeiignhgt2c2h5%anagbeosvewetrheatnoofttahceccoomnptaronlierdatsby14modrapyhsollaotgeir.caTlhcehsaenges. Tohweerpatnhcarneatthiocseweoifgchotnstrwoelrseastltihgehtelnyddeopfrethsesetde,stbaenidngr8ec%ovaenrdy 126 pihnacsreesa,serdesipnewcetiivgehlty.aftTehrethaedrleasntaldsosaen,d+t1es4t%es awnedre+1sl1i%g,htly DreusPpoecnttivCeol.y,(nbdu.t).retUunrpnuedbltiosnhoerdmDaalta1.4 days later. Type: 14-Day Feeding Study SSpeexc/ieNsu/mSbtreari:n:~~~~ RMaalveC/r2l0/:gCrDouBpR Exposure Period: Frequency of 14 days; 56 days of recovery Treatment: Exposure ad libitum for 14 days Levels: Method: 0,30, 300 ppm Rats were approximately 6 weeks old at arrival. At study start, the `baonddywewreeigfhetdsfroaondgeadndfwraotmer1a9d60li2bi4t0umg.. Rats were housed singly During the test period teeascthsugbrsotuapncwee.reDifeetdsdwiectrethpartecpoanrteadinoendce0,f3o0r,thoer 23-0w0epepkmfoefedtihneg wpeeriigohdeadnadndweorbesesrtovreeddfroerfcrliigneircaatledsiugnntisloufsteodx.icAitlyl. raRtastwsenroet osabcsreirfviecdeddautrithneg end the roefctohveerfyeepdeirnigodp.erCiaogdewseirdee weighed and examinations to rdeattesc/tgrmooupriwbeurnedsoarcrdiefaicdedraatts twheereencdoonfdutchete1d4t-wdiacyefdeaeidliyn.g Fpievreiod, wanedigohnedreactoevaecrhy dsaacyrsif7i,ce28p,erainodd.56B.loLoidvesrasmwpelrees rweemroevteadkeannfdor eorngdanoofftlhueorfiedeedicnogncpeenrtiroadtaionnd aonnalryesciosvefrryomdarayts7.sacrificed at the GLP: Test Substance: Results Appropriate body weight sgtaaitnisst,icaanldmoertghaondswewiegrhet used data. to analyze body weights, No DAimetmsowneiruemnopterafnlauloyrzoeodc.tanoate, purity approximately 100% tNooximciotrytanloittieedsiwnetrhee o3b0searnvde3d0d0urpipnmg tghreousptusdyd.uriCnligntihcealfseiegdnisngof dpiesrcihoadrgien,claunddedhuirnrcehgeuldarporsetsuprier.atiSotna,tirsatpiicdalblryesaitghniinfgi,carnetddneacsraelases ginromuepaonnbdoadyys w7eiagnhdts14weofrethoebsfeeerdviendgipnerraitosdfarnodm otnher3ec0o0vperpymddaoyse s7.ignMiefiacnanbtloyddyewperiegshsetdgadiunrsinfgrtohmetfhiisstswaemeekgorfoufpeewdeinrge. iSnigrnaitfsifcarnotmitnhcere3a0seasndin3m0e0apnpambsdoolsueteglriovueprswesiacgrhitfsicweedraet othbeseernvded ionfctrheeasfeededmienagnperreiloatdivaendliovenr recovery weights days were 7 and 28. observed Significantly in rats from the 30 and 300 ppm dose groups sacrificed at the end of the feeding spaecrriiofdicaenddoonnrreeccoovveerryyddaayy278,. and in the 300 ppm dose group rats Reference: rMeecaonverbylodoadyo0rgwaenrofel0u.o3ripdpemcoinncceonnttrraotliornasts,of33ra.t2spspacmriifnictehdeon `3m0eapnpmblroaotds,oarngdan7o1f.l5uoprpidme in the 300 ppm concentrations rats. were On 0.9 recovery ppm for day 7, controls, 300 ppm g1r9o.u3p.ppm for the 30 ppm group, and 22.2 ppm for the DuPont Co. No. 326.95. (1995). Unpublished Data, Haskell Laboratory Report Type: SSpeexc/iNeus/mSbterra:in: Exposure PeFrrieoqude:ncy of Treatment: LeEvxeploss:ure Method: GLP: Test Substance: Results: References: 14-Day Feeding Study ~~ MMiaclee/aCnrdlf:eCmDal-e1/S/group. 14 days ad libitum for 14 days M0.a3l0e,a3n0d0,fe3m0a0l0epmpicme (age 43 and 44 days, weighing 21 to 38 g) w1e4rceonfseedcduiteitvsecdoanytsa.inMianlgeaamnmdofneimuamlepceornftlruoolrsoowcetraenooabtseefrovred cfoonocducrorennstulmyptainodn,feadnodncllyingircoalunsdigcnhsoww.ereInrdeicvoirddueadl. bLoidvyerwewiegihgthst,s were No recorded at the 14-day sacrifice. AAlmlmmoanleiuanmdpfeermfaluloeromoiccteandoiaetde,afptuerridtoysaipnpgrwoixtihma3t0e0l0y p1p0m0.% One afnedma3l0e0m0opupsme. diLeidveatr 3w0e0igphptm/.bodByodweyiwgehitgrhattiloosssshoocwceurdreaddoatse3-00 sriegsnpsonosbeseirnvceredasiencaltu>de3d0unpkpemmipnt mhaelaed aanrdeaf(e3m0a0leanmidce3.00C0limngi/ckagl) aDnudPownetakCnoe.s(s19(8320)0.mgU/nkpgu)b.lished Data, Haskell Laboratory Report No. 12:82. Kennedy, G. L., Jr. (1987). Toxicol. Lett. 39:295-300. Type: 14-Day Feeding Study Species/Strain: Sex/Number: ~~ Mice/Crl:CD-1 Male and female/S/group/gender Exposure Period: 14 days Frequency of Treatment: Exposure Levels: Method: GLP: Test Substance: Results: ad libitum for 14 days 0, 10, 30, Male and 100, 300, 1000, 3000, 10,000 ppm female mice (age 41 and 42 days, weighing 19 to 30 g) w1e4rceonfseedcduiteitvsecdoanytsa.iniMnagleamamndonfiemuamlepecrofnlturoorlosowcetraneooatbesefrovred fcoonocducrornenstulmyptainodn,feadnodncllyingircoalunsdigcnhsowwe.reInrdeicvoirddeuda.l boLidvyerwewieigghhst,s were recorded at the 14-day sacrifice. No DAemamtohnoiccuumrrpeedrfaltu1o0r0o0o,ct3a0n0o0a,tea,npdur1i0t,y0a0p0pprpomxi.maAtlellyfe1ma0l0e%mice at 32-0w0e0epkptmimaendpoialnlt.maAllel m1i0c,e00a0t p1p0,m00f0empaplme mwiecree adnedad30at00thpe pm omcacluerrmeidceinwtehree1d0e0a0dpapt mthemi1c-ew,edeektatilismeonpoitnhte.nuAmlbtehrouogfhddeeaatthhs was not reported. Reference: Body 3000, weight loss and 10,000 pocpcmurmraeldeatanthdefeenmdaloef cach mice. weLievkeratw3e0i0g,ht10b0o0d,y waenidgfhetmraalteiomsicseh.owCeldinaicdaolssei-grnesspoobnsseerviendcrienacseluadte>d w1e0apkpnemssin(m1a0l0e 3100000,0anpdpm3)0,0t0rpepmmor)s, (p3al0l0oran(d110,000000ppppmm)),, psitlaoicnreedcpteiroinn(e1a0l0a,rea (100-1000 ppm), weight and unkempt appearances loss (300 (100, 1000, 3000, -- 10,000 ppm) and 10,000 ppm), DuPont Co. (1981). No. 560-81 Unpublished Data, Haskell Laboratory Report Type: Species/Strain: Sex/Number: Exposure: Period: Frequency of Treatment: Exposure: Levels: Method: 14-Day Feeding Study Mice/Cri:CD-1 Male and female//group 14 days . ad libitum for 14 days ANomnmaodenciauflmuoprerofdleucoarnooocitcanaociadt:e:3,0,103,030p,p3m00, 3000 ppm Mpeirxftluuroersooocftannoonaatdee:ca1f5l/u15o,ro5d/e2c5,an2o5i/c5 apcpimd/ammonium Male diets and female mice containingcither a(amgmeo4n4iduamysp,ewrfeliugohrionogct2a4no1a0te3,5 g) were fed snuobnsatdaenccaefslufoorro1d4eccaonnsoeiccuatciivde, or mixtures days. Male of the two test and female controls were observed concurrently and fed only ground chow. Individual body GLP: Test Substance: Results: Reference: rweeciogrhdtesdaantdtchlein1i4c-adlasyigsnascrwifeircee.recorded. Liver weights were No LAimvmerowneiiughmtp/ebrofdlyuowreoiogchttanroaattieo,sphuorwiteydaapnprionxciremaasteelaty a1ll00le%vels etensltaerd.gemMeinxtinagpptehaer2edtetsotbseubdsotsaen-creesladtieddnwoitthalter the effects. Liver snuobnsatdaencceasf.luorodecanoic acid appearing the more potent of the two DuPont Co. No. 537-82. (1982). Unpublished Data, Haskell Laboratory Report Type: Species/Strain: Sex/Number: Exposure Period: Frequency of Treatment: LeEvxeploss:ure Method: GLP. Test Substance: Results: References: 2M1i-cDea/yCrFIee%CdDi-n1g Study Male and female/S/group 21 days waidthloibuittcumomfporou2n1dd,ayfsor(2Midcaeysw)ere mistakenly fed ground chow, 0M,a0l0e1a,n0d.0f3e,ma0l.e1,m0i3c,e 1,3, (age 10, 41 30 ppm and 42 days, weighing 21 10.33 g) were fed diets (suspended in 1c9o%ntcaoinminogilabmemfoorneibueminpgemrfilxueodroioncgtarnoouantde chow) for c2o1nccuornrseenctultyivaenddayfse.d oMnalylegraonudnfdecmhaolwe.coInntdriovlisdwuealreboodbsyewreviegdhts athned2c1l-indiacyalsascirginfsicwee.re recorded. Liver weights were recorded at No ALimvmerosnwieurem spiegrnfilfuiocraonotlcytahneoaavtiee,rpautri3t0ypappmpr,osxliimgahttleylyhe1av0i0e%r at 3 ppm, and No changes aipnpleiavreerdweniogrhmtalweart e10sepenpmat in male and female feeding levels of 1 mice. ppm or lliogwhetro,fnanod dtihfefseirgennicfeiscaatnc1e0opfptmh,e cishqaunegsetioobnsaeblrev.edTahte3 opnplmy,cliinnical sDiugPnoonbtseCrov.e(d19w8a2s).spoUrnapduibcliwsehiegdhtDalotsas,. Haskell Laboratory Report No. 323-82. Kennedy, G. L., Jr. (1987). Type: 2-Year Feeding Study SSpeexc/ieNsu/mSbtreari:n:~~ MaRlaev//1C5r6l/:gCrDouBpR Exposure Period: 2 years Toxicol. Lett, 39:295-300, 20 20 Frequency of Treatment: Exposure: Levels: Method: GLP: adlibitum for 2 years 0,0 (pair-fed), 300 ppm (Mcaolnetrroat)s,w0er(econatdrmoilnipsatierr-feeddctiotthhee0ra(mamdolniibiutmumpceornftlruoolrogoreotuapnoate gdircotufpor(CaPp-pCrSo)x)i,maotre3l0y02pypeamrsa.mAmfotneriuasmsipegrnfmleunotrtooocttraenaotamteentin the `(g1r0o/ugprsoeupatcthirmaet pwoainst)d,escielglnaptreoldiffoerraetiitohnerevhaolrumaotinoanl (e6v/aglruoautpi/otnime point), point). or evaluation All rats were of peroxisome provided food parnodlitfaeprawtioan a(6td/glrieobuiptrfutmi,me aSntaabliylsietsy oatftahmemboegniinunimngp,ermfildudolreo,ocatnadnoeantdeowfatshecosntfuidyr.med by dTihertoaungdhotuhet hthoemosgtuednye,ictoynocfetnhtreattiesotnodifetthsewetresetdceotmerpmoiunendd. in the sAtlalrt.ratBsowdeyreweaipgphrtosx,ifmoaotdelcyo4n9sudmapytsioofn,aagnedocnlitnhiecadlasyiogfnsstwuedrye monitored throughout the study, 6B.l9o,od12w,a1s5,co1l8l,ecatnedd2f1ormhoonrtmhosnaafltearnainliytsieastiaotn aopfptrhoexistmuadtye.lySe1,r3u,m fwoalslicalneasltyizmeudlaftoirntgeshtoorstmeornoene,(FeSsHtr)a,diaonld, lpurtoeliancitziinngcohnocernmtornateio(nLsH.), R1a2,ts15w,er18e,saancrdif2i1cemdoantththseffoorlcleolwlipnrgoliinfteerraitmiotnimaendpopienrtso:xi1s,o3m,e6, 9, ptersotleisf,eeraptiidoindyevmaildueasti,oansc.cesTshoeryfsoelxlogwlianngdo(rAgaSnGs)wuenrite wwietihghfeludi:d, cliovaegr.ulaItmimnegdigalatnedl/yseafmtienrawleviegshiicnlge,wtihtehlfilvueirdarnedmotevsetdes, fprroosmtate, and palnaicmeadlsinseilceec-tceodldfohrompoegreonxiizsaotmieopnrobluifffeerratfioornpeevraolxuiastioomnalwere psreelpeacrtaetdiofno.r cTelhlepfroollilfoewriantgiotnisesvuaelsuawteirone:corilglhetctaenddflreoftmttehsetesr,ats egrpoisdsidlyemsiiodness., AAlSlGr,atslivseurr,vdivuiondgetnhuem2,4p-imtouinttahry,teastndpearliloodrgwaenrsewith sunaictr,ifciocaegdulaantdinngecgrolpasnidc/ds.emiBnraalinv,eshieacrlte,s liver, with fslpuliedern,emkoivdendey,s, ASG plirvoesrt,attees,teesp,ideipdiydmiiddyemsi,deasn,dptaensctreesaws,eraendweoirgghaends watitnhecgrroopsssy.lesTihones were examined microscopically. Data No were analyzed by appropriate statistical methods. ReTseuslttSsu:bstance: Reference: AInmcmreoanseidurmelpaetirvfeluloirvoeorcwteaingohattes,wpeurreitoyb9se8r-v1e0d0i%n the ammonium pearlfsoluionrcoroecatseadnoiantteh-etraeamtmedonriatus.m Hpeepraftluiocr8o-oocxtiandoaattieo-ntarcetaitveidtyrawtas sat sailglntiifmiecapnotilnytsa.lteArmthmeornatieuomf LpeeryfdliugorcoelolctoaxniodataetidiodnnoortLeydig cell aprpoplriofxeriamtaitoen.lyM2o0r-etoivmeers,letshse trhaatneofthBe-oraxtiedoaftihoepnatinicLe8y-odxiigdacteilolns.was uSnecrhuamngteeds.tosTthereornee,weFrSeH,,hporwoelvacetri,n,siagnndifLicHantleivneclrsewaesersein serum estradiol 1,3,6,9, levels in the 15, 18, and ammonium 21 months. pAetrf1l2uomroonotchtsa,notahtee-atmremaotnediuramts at `pewrhfelnucooromopcatraenodatteo-tthreeaptaeidr-rfaetds choandtreolle.vaHtiesdtospeartuhmoleosgtircaadliol levels ceevlall,uaantdiopnanrcerveeaatliecd accoimnpaorucnedl-lrteulmaotresd. iBnacrseeadseosnitnhleisveerd,aLtae,ydthieg eLsetyrdaidigolcellelvtelusmoarnsd arpepdeuacredtoprboeladcuteintoletvheelscombinationof elevated DuPont Co. (2000). Unpublished Data (Draft Manuscript). Type: Species/Strain: RReaptesa/tCehdR-ECxDposure Inhalation Study Sex/Number: ~~ Male/20/group Exposure Period: Frequency of 2 weeks; 42 days of recovery Treatment: Exposure 6 hours/day, 5 days/week LMeevtehlos:d: H0o,u8s,e8l0inmega/imr'(approximately 20 L/min) was passed through a acgyietlaotnoer--rheesaedrvdouisrttgheatnecroanttoarinceodnnaemcmteodntiouamppaerrtifcllueorooctanoate. e`Txhpeosreusrueltcihnagmabierrb.orTnehepaarttimcouslpahteerwiacscopnacsesnetdraitnitoona o3f0aLmmbaotnteiruymjar perfluorooctanoate in 30-minute intervals. the exposure chamber was monitored at Rats day. wAefrteeeSrxpdoasyesd,,thheeardatsonwley,refogriv5ecnoans2e-cduatyivreecdaoyvse,ry6 hours per (weekend), rwahtis cwherweaswefioglhleowdeadnbdyo5bsdearilvye,dcdoanisleyc(uetxicveep6t-wheoeukreenxdpsos)urdeusr.ingAltlhe {eixbpiotsuumraetaanlldtriemceosveorthyeprertihoadns.durFionogdthaendacwtauatlerexwpeorseuraev,ailable ad eCalicnhicgarloulpabaotra0t,or14y,eaxnadmi2n8atdiaoynsspwoesrt-eexppeorsfuorre.medAfotner10a rtaottsalforfom a1t00e,x1p4o,s3u2r,esa,n5d a4t2sdfaryosmpeoastc-hexgproosuuprew.erOergpaatnhsolwoegriecawleliygehveadl,uaatnedd absolute and relative organ weights were calculated. GLP: Test Substance: Results: `Ognrouthpef5or*eaynede9xa"mdinaaytsioonfse.xposure, 10 rats were selected from each No OAvmemraolnlimuemanpecrofnlcueonrtoroacttiaonnosatwee,rpeu0r,it1y1app5r,o8x3im=at1e7lyfo1r0t0he%0, 8, and 80 mg/m' exposure levels, respectively. Rats exposed to ammonium perfluorooctanoate showed a gsrupopurpeswsaisonmoorfebosdevyerweeliyghatfftehcrtoedugdhuoruitngtheextpeosst.urTehaen8d3fomrg/14m'days pofawriencgov,ercyh.ewiDnugr,inagndearcehd eexypeoasnurden,assaplordaidsicchacragseeswoefreblsienkeinnign, all groups. e`lSeovmaeterdataslekxalpionseepdhtoospahmamtaosneiauctmivpietryf.luEofrfoeoccttsawneoarteesdeuesnt idnisplayed t`grlauntsaammiicn-apsyeruavfitcert0r,an1s4a,mainndas2e8arnedcogvleurtyamdiacy-so.xalTahceetiinccidence was related to dose; higher activities were found at the higher exposure dleivfefle.renHtowfervomerc,onotrnollys.the latter activity at 83 mg/m' was statistically dPeagtehnoelroagtiicoenvaolfuhaetpiaontsocsyhtoesweidn tchleoluidvyersswoefllriantgs eoxrpgorsaendultaor eafmfemcotnwiausmnoptersfeleunoraofotecrtaano1a4t-edafyoror10lodnagyesrwrietchovneoryrepceorvieordy.. This No roetlhaetredcoimnpcorueansde-rienllaitveerdwheiisgthotlsogwicaschoabnsgeervsedw.ere noted. Exposure- References: eNxoamciomneeadl,aftietri5,oorr9coenxjpuonscutrievadlayesf.fects were scen in anyof the rats DuPont Co. (1979). No. 253-79. Unpublished Data, Haskell Laboratory Report Kennedy, G. L., J. etal. (1983). TThoexicologist, 3:22. Type: Species/Strain: RReaptesa/Ctreld:CEDxposure Inhalation Study Sex/Number: Exposure ~~ Male/24/group Period: 2 weeks; 84 days of recovery Frequency of Treatment: Exposure: LMeevtehlos:d: 6 hours/day, 5 days/week bD0,yus1pt,a8sa,stim8no0gsmpahigre/rtmeh'srooufghamamgloansisugmenepreartfolru.orFooocrttahneoahtieghwecroencgeennterraattieodn d(e8t0emrgmi/nme'd,bcyhgarmabveimreattrimcosapnhaleyrseisc.onFceonrttrhaetiionntserwmeerdeiaptreimaanrdillyow wcoenrceendtertaetrimoinnse(d8baynda c1hmegmi/cma'l, arneaslpyescteisv.ely), chamber atmospheres hMeaalde-ornatlsy(toagdeu7s-t8awtemeokssp,hewreeisghfoirng6 2h4ou0r-s2/7d9ayg,)5wedraeyse/xwpeoeskedfor a2nwdecelkisnic(awleseikgennsdwseerxeclnuotdeedd.). PDoustr-ienxgpoesxuporseurraet,s rwaetrsewewreeigohbesderavnedd 2obtsiemrevse/dwdeaciklythfrooru1g4h r8e4codvaeyrsyodfaryesc,ovtehreyn.weFiigvheerdatasn/dgroobuspewrevrede tseasctridfaiycse.d at 0, 14, 28, 42, and 84 daysofrecovery, for a total of 96 aClcihnicgarloulapbaotra0t,or14y,e2x8a,mi4n2a,tainodns84wedraeyspeporsfto-rcmxepdosounre$. raAtfstferraomtotal eovfa1l0uaetxepdosautr0e,s,14, rats 28, from each 42, and 84 dgaryosuppowsetr-eexppaotshuorleo.giTcahlelyrats were eevxaalmuaitnieodn.grIonssaldydiatnidont,islsuunegss,anhedarotr,gatnhsymwuesr,essplaeveend, floirvemri, ctreostsecso,pic and kidneys were weighed. GLP: Test Substance: Results: At necropsy, blood was collected for analysis of organofluoride. wleevreelsainnalryatzebdloaotd.eacBhloroedcosvaemrpylpeesrifodr.omBtlhoeo0dasnadmp8l0emsgf/rmo'm gtrheoups d1aaynsd 8 mg/m' groups were analyzed only at 0 and 28 recovery No OAvmermaolnlimuemanpecrofnlcueonrtoroacttiaonnosatwee,rpeur1i.t0y0a.p5p,rox7i.m6a.t2e.l5y, 1a0nd0% 83.9 12.8 mg/m' for the 1, 8, and 80 mg/m' groups, respectively, Body weight analysis demonstrated no significant differences between controls and | mg/m. Body weights from animals exposed to 8 mg/m' were significantly higher than controls from test days os1if7g-cn3li3if.niiccaBanlotdlsyiyglnwosewidegurhrtitshnagfnercxoopmnotasrnuoirlmseasflsrshoeomxwpteeosdsteoddnalytyos s82l0-i1gm6h.tg/sOmibg'sneswreovrfaetniaosanls 3an4d doacyuslaornditsescthaIrgraet. diHeodwedvureirn,gaetxtphoesuhirgehacnodnc1enrattrawtaisons,acarftiefriced winasexptrreombiasb.lyBeoxtphosoufret-hreesleartaetds,haaldthsoeuvgehrepawtehioglhotgilcosse.valMuoarttiaolnity `could not determine the cause of death. Three of 24 rats in the e8x0pomsgu/rme'peerxipoods.ure group exhibited lung noise during the. 12-day `inOcrrgeaansteoibnolduyngw,eliigvhert, raantdiostedsetemsonwsetirgahttesdafatserig0nirfeiccaonvte,rydodsaey-sr.elaTtheed liver/body weight ratios were significantly higher in animals exposed to 80 mg/m' through 28 days of recovery. Mean absolute ltihvreoruwgehi2g8htdsawyesroefsriegcnoivfeircya,ntbluythithgihsermainy tbhee a8nmagr/timfa"ctanciamuaslesd by an unexplainable increase in body weight accompanied by a normal increase in liver weights at mg/m'. Calliknailcianle plhaboosrpahtaotraysemeiansaulrlegmreonutpss deexmpoonssetdrtaoteadmamnoinnicuremase in perfluorcoctanoate after 10 exposures, but this finding was significant only at 8 and 80 mg/m'. This increase persisted in the 8840 dmagy/smo'farneicmoavlesrythnrooudgihffe1r4edncaeyss woefrreecfoovuenrdy.. After 28, 42, and pCaonmipoobuunlda-rrheelpaatteocdelplautlhaorlohgyipcerftirnodpinhgys, icennctlruodleodbhuleaarvyhelpiavteorcs,ellular hypertrophy, and hepatocellular nerosis in animals exposed to 8 and 80 mg/m'. These findings showed an exposure-response relationship, but were or 42 days of recovery reversible by (80 mg/m'). 28 days of recovery (8 mg/m') References eBxlpooosduroer-graenloaftleudoprriedseeanncaelyisniasllclgeraorulypsde(mioncnlsutdriantgedthaencontrols, this dfeicndrienagserdemwaiitnhstuinmeex,pbluaitnwead)s.deBtelcotoadbloergafatneorfl$u4ordiadyesolfevreelcsovery in D`buoPthonthteCcoo.nt(r1o9l81a)n.d 8U0npmugb/lmis'heexdpoDastuar,e Hlaesveklesl.l Laboratory Report No. 205-81. Kennedy, G. L., Jr. ctal. (1983). The Toxicologist, 3:22. Kennedy, G. L., Jr. 24:13251329, ct al. (1986). Food Chem. Toxicol, Type: Species/Strain: Repeated Dose Rats/ChR-CD Dermal Study Sex/Number: ~~ Male/1S/group Exposure Period: Frequency of Treatment Exposure Levels: Method: GLP: Test Substance: Results 2 weeks; 42 days of recovery 6 hours/day, 5 daysiveek 0,20,200, 2000 mg/kg pMraelveenrtatisn(gaesgtei8onowefetkhse, cwoeimgphoiunngd2w10h-e2n45prge)enwienrgeacnodllgarreodomtoing pdeurrfilnugortohoec2t-anwoeaetke,exapsoasnuraequpeeroiuosd.pasAtem,mwoansiaupmplied to the backs of eeancdhedrawthtehant thhaedcboemepnosuhnadvewdasfreweipoefdhafirr.omDtahielyra6t-s'hobuarckesxpwoistuhreas `gTahurzoeugpahdo.ut Ctohlelatersst wpeerrieodr,efmooovdedanadftwerateexrpowseurreeadvaayila10b.le ad libitum. Body weights and clinical signs were recorded. tAafkteenr efxrpoomsSurreatdsafyro1m0 acancdhognrroeucpovfeorryhedmaaytso1l4ogayndme4a2s,urbelmoeondtwsa.s oGnro5ssrantesc/rgoropuspy aafntderheixsptoopsautrheol1o0g,iacanld eoxnamriencaotvieornysdawyesre14pearnfdor42m.ed Mpeerafonrmaebds.oluAtfetaerndexrpeloastuirvee o1r0gaanndtoonbordeycowveeirgyhtdaaynsal1y4seasndwe42r,e bdeltoeordmiwnaasticoonlsl.ecEteydeferxoammi$narattiso/ngsrowueprfeorpeorrfgoarnmofelduoonrienaech rat after einxcplousduerdeg9r,oassndobosnerrveactoivoenryofdathyese1y3esanudsi4n1.g aTbhreigphrtolcieghdtu,raend semimicroscopic observation slit-lamp biomicroscope. using a hang magnifying lamp and a Data No were analyzed by appropriate statistical methods. RAamimsotnreiatuemd wpietrhfl2u0ormogoc/tkagnoaatmem,opnuiriutmy appeprfrlouxoirmoaotcetlayno1a0te0%showed enxopremrailmebnotd.y Dwueriignhgtsthaend10n-odauynuesxupaolsculrienipcearlisoidg,nrsatdsurtrienagtetdhewith either 200 or 2000 after the exposure mg/kg period. lost weigh, followed Slight redness of the by a skin normal growth was observed in these 2 groups, along with salivation in the 2000 mg/kg group only. pClhionsipchaaltaesnez,yGmPeTd,etaenrdmiGnOatTi)onsshmoowneidtodroisneg-rleilvaetrefduinncctrieoans(easlkinalailnle treated groups recovery days after exposure 14 and 42. 10. These values returned to normal ac Liver damage characterized all treated groups following by the congulative necrosis was 10 dose. The incidence observed and in 35 36 severity ofliver damage was dose-related. Recovery was complete eisnstehneti2a0llmygc/okmpglgertoeuipn 1t4hed2ay0s0 fmogl/lkowgignrgotuhpe a1t0tTMhedossaem,eantidmew.asOn irnectohvee2r0y0d0aymg4/2,kgregvreoruspa.l oTfwlioverratdsaemxapgoesewdatsoe2ss0e0n0timagll/ykcgohmapdlete c1o0aegxuploastuirveesn.ecLriovsiesr owfeitghehse,pibdoetrhmiosn aatntahebsdoolsuetesiatnedforelllaotwiivengto b10o,dwyiwtehiagrhettubarsnist,osnhoorwmeald waediogshet-rseeleanteidn itnhcer2ea0smego/nkegxpgorsouurpeatday i1n4crdeaayssedanldivienr twheeig2h0t0pmegrs/iksgtegdrfoourp4a2td4a2ysdaiynstohfe r2e0c0o0vemrgy./kTghe dgaroyup1,0,atlhtehomuegahnaktirdennedytowweairghdtnsoorfmtahlew2a0smogb/sekrgvegdr.oupOnweerxeposure Ksiigdnniefyicwaentilgyhtgsrefarteormtthhaen2c0o0nt0romlgs/,kagndgrtohuepmweearneasbisgonliuftiecasnptllyeelnesasnd t2h0a0n0cmongt/rkolgs.groTuhpe smheoawnerdelaasttiavteistteisctailcluylasrigwneiifgichatnstfirnocrmetahsee on esixgpnoisfuicraentdaiync1r0e.asOe ninrtehceomveerayndraeyla1t4i,vetkheirdenweyasanadsttaetsitsetsicwaelilgyhts of the 200 and 2000 mg/kg groups. References: eBxlpooosduroergdaanyof1l0u,orfiodlelolweveedlsbyshaodweecdredaossee-irnetlhaeteldoveellesvaattiroencoonvery d5aa2ny,ds8811,p4apanmndd(214012,8.2p0Tp0h,mesa(en2d0v,a2l20u00e00s, amafgnt/dekr2gt0,h0er0e1sm0pge"/cetkxigvp,eolrsyeu)srpaeetcrtraienvcgeolevyde)rfytroodma1,y4, 4120.ppAmt,a tbhleoreodweorrgeannoofrlmuaolrildievecrowneciegnthrtattoiobnoodfy awpepirgohxtimraattieolsyand sdiefrfuemreencnezsyfmreoamctciovnittryo,lsa.ndOnnoecrlaitnischalowseigdnsliovrerbcohdayngweesigahttthis tlheveerle. weArteambalrokoeddorlgiavenrofcihuaonrgiedse alnedvelsiogfniafpipcarnotxiimnacrteealsyes50inptphme, `mean were eavbisdoelnutteatatnhdisrbellaotoidveorligvaenrowfeliugohrtisd.e No other toxic concentration. effects DuPont Co. (1980). No. 589-80. Unpublished Data, Haskell Laboratory Report Kennedy, G. L., Jr. (1985). Toxicol, Appl. Pharmacol, 81:348-355. Developmental Toxicity Type: Developmental Toxicity in Rats Species/Strain: ~~ Sex/Number: ~~ FReamtasl/eCsr/l6:-C1D5/Yg(rSoDu)pBR Route of Administration: Inhalation Exposure Period: Frequency of Treatment: Exposure Levels: Method: Days 6-15 of Gestation 6 hours/day F0,e0m.a1l,e1r.a0t,s1w0e.0r,e2m5a.t0emdgt/omm'ales, and mating was verified by fdoeltlecotwiionngoofvesrpneirgmhattcoozhoaabiitnattihoen.vagTihnaeldlaayvathgaetesapcehrmmoatronzionag were detected was designated as Day 1 of gestation (Day 1G). 2F4ormEaxtpeedrfiemmeanltesI (wfeermealteoshsaavceribfeiecendabsesfiogrneepdtarotueraitcihong)r,oauptotal of (12 females/group/Run). However, duc to the degree of maternal toxicity in evidence in the concentration was reduced 25.0 mg/m' group to 10.0 mg/m' for in Run Run II, I, this and 15 mated fcoenmtarloelsgwreoruepsas(s6imganteeddtoftehmiaslnese/wgrgoruopu)p.weFruertahdedremdorteo,RtuwnoIIm;oornee was the pair-fed to the 25.0 10 mg/m' group. mg/m' group, and the other was pair fod to F1o2rmEaxtpeedrfiemmeanlteIrla(tfsewmearleesdaisltlroiwbeudtetdotgoicvaecbhirgtrho)u,p.in RWuinthI the additionofthe 10.0 mg/m' group added to both the control and the in Run II, 6 10.0 mg/m' mated females groups. were pReartfslwuoerroeocetxapnoosaetde winho1l5e0-Lbogdlyastsoaantdmosstpahinelreesss sotfeealmRmoocnheisutmertwyipree-cmheasmhbemrosduwlietsh.inCwhhaimcbhetrhecornatcsenwterraetihoonussweedrienddievtiedrumailnleydinby gravimetric analysis either each % hour (1.0 mg/m', and 10 or 25 mg/m') or cach hour (0.1 mg/m'), and by a spectrophotometric teexcphonsiuqrueed(aoyn feoarcthhe0.o1thmegr/lme'veslasmtpelset,ed)a.nd on 5-6 samples per cDoanmssumwpetrieonwewiagshemdeaasnudreodbsdeurrviendgfgoerstclaitniiocnal(2sifgenmsa.leFse/ceadge). The ddaatmaswewrereecocloldeectdedf,roamndbeufntoirleaslalcsrtirfuiccteuruanltiallatlelramtaitoenrsnnaoltaenddamfeotnalg. ethxeamfietnuesdesfworergerocslsaspsaitfhioedl.ogAifcicehrasnagcersi,ficlei,vetrhweediaghmts wwaesrerecorded, aluntdeareapnrdodiumcptliavnetasttiaotnussiwtaess wdeerteercmoiunnetde.d,Thanedntuhmebneurmobfercoarnpdora position of all live, dead, and resorbed fetuses were recorded. All live and dead fetuses were weighed and sexed externally and GLP. Test Substance: Results: ialntteerrantailolnys,.anAdpptrhoexliimvaetfeeltuyse%s owfertheeefxeatumsienseodffeoarcehxtleitrtnearlthat were. aalltievreatwihonesn, ranedmoavlledstfunrtoemdtohremdaalmfowremreedefxeatumsiensewdefroerevxisacmeiranled swiemrielarallys.o eTxhaemifneetudsefsorthhaetawdearleteeraxtaimonisn.edSefcotriovnissc,ecraolntaalitneirnatgitonhse e2yfeestuose3fs ffertoumsecsacfhrolmitteearcohf ltihtetercoofnttrhoel g2r5o.u0pm,gw/emr'e gprroocuepsasendd of histologically for examination. In Run IT, one fetal head from cach oefxa4milnitetde.rs fIrnoamddtihteio1n0,.0thmegh/eamdsgrforuopm aalnldftehteusceosntirnotlhegrgoruopupwepraeir ffeetdusteosthweer2e5 emxga/mmi'negdrofuopr swkeerleetpalroacletesrsaetdiofnosr. examination. All FsoarmeExapsefroirmEexnpterIIi,mtehnetprI,oceexdcuerpetsthuastetdheuntdialmDsawye2re1Gwewiegrheedthleess afnredqutheentildyendtuirtiyongfegeascthatioofnf,spfreiendgcwointshiunmpltititoernswwaassnnoott mreetaasiunreedd., pBoelfyocraerebxopneatcetecdagpaerttuhraitticoonn,taeiancehddbaedmdiwnags. hTouhseeddatineoa.fparturition was noted examined and was termed for clinical signs. Day 1 For PP. each The test dams group were weighed fertility index and and agensdtaltaicotnatiinondeixndweexrewecraelccuallactuelda,teadn.d for All edaacmhsLiwteerrevisaabcirliiftiyceidndoexn. Day 22 PP. ``eThxeampiunpesdffroormexctaecmhaldaamltewrearteioncsoutnotweadr,dsethxeede,nwdeoifghDeady, a1 nPdP. `Thereafter, cach clinical signs on pup Day was weighed 4, 7, 14, and and inspected for adverse 22 PP. The eyes of the pups in all groups of Run I ophthalmologist on (Experiment Days 15, 16, Il) or w1e7rPePe,xsahmoirtnleydabftyeratnhe eyes ocopdeende.d.OTnhiDsaeyx3a5miPnPa,ticoanchwapsupcownadsucstacerdifwiictehd tahnedetxhpeoseyuerse wleevreels fixed for possible future evaluation. Data Yes were analyzed by appropriate statistical methods. `ATmhemaocntuiaulmmepaernflcuoonrcoeoncttraantoiaotnes,apcuhriietyve>d9w5er%e 0 and approximately 0.14, 12, 9.9, and 21.0 mg/m' for the 0, 0.1, 1.0, 10.0, and 25.0 mg/m? exposure groups. No effects were observed at 0.1 or 1.0 mg/m'. None of the 2di1edd,abmust etxhpeyossehdotwoeadmsmimoinlairucmlipneircafllusoirgonoscttoanaoaletseseatr d1e0g.r0emegt/hma'n ptehartflsuecernooatcttahneo2a5t.e0wmasg/omv'erltelvyelt.oxiActt2o5ramtgs/imn't,haatm5m/o24nidiudmnot bsurrovwinveditosctoelromr,atmioosntaorfoutnhde tshuerveiyveosr,snhoasde,waentdamboduotmhe;nsl;etrheardgdyi;shedxepcoresausreedpfeerieoddc;oannsdumapntuinonkeamnpdtbaopdpyeawreaingchet. gain during the bMaastiesrinnadliclaitveerd wtehiatghetxpcohsaunrgeestoaammomnogngiruoumpspeornflauorreoloatcitvaenowaetieghatt i1n0c.r0eamsge/imn'liovrergrweeaitgerhtreoscuclutrerdeidn dseisgpniitfeictahnetlsyiglnairfgiecranlitvedres.creTahsiesin rbeoldatyivweeilgivhetrgwaeiinghitnstohfetsheegrpoauiprs-,fewdhciocnhtrsoilgngirfoiucpasn.tly reduced the iNmopldainftfse,remnecaesn fnruommbceornotfroclorwpeorreaolbusteera,veodrifnettalhedemaetahninnuamnbyedrosoef level tested. dDaevmesloopnmDeantya2l1tooxficgietsytawtaiosnnaottadneymoconnscternattreadtiuopnoonfsaacmrmifoinceioufmthe tpoexrifcliutoy,roeoxcptraensosaetde atsesdteedc.reCaosnecdenfettraaltiwoeni-grhetl,aotcecduermrberdyoo-nfleytaatl b2o5.d0ymwge/img'h,t wpehriscihstwedastoovDearytly1 tPoPx,icbuttontohtetdoaDmsa. 4yThPiPs.decreased On Days vivo from 15, 16, or Run Idid 1n7otPPr,evceoaldecdonecxeanmtriantaitoino-nreolfattehde pups" eyes in malformations. examination of In the view eyes of of these negative the pups from RruesnulItfs,wsaisminloatr in vivo conducted. Reference: hAamzmarodntioutmhepceornfcleupotruoso.ctanoate did not demonstrate a unique NDou.Po8n81t-8C1o.. (1981). Unpublished Data, Haskell Laboratory Report Staples, R. E. et al. (1984). Fundam. Appl. Toxicol., 4:429-440, Type: Developmental Toxicity in Rats SSepexc/iNeusm/Sbterra:in:~~~~ FReamtasl/eC/r2l5:/CgDr*o(uSpD()EBxRperiment I), 12/group (Experiment II) Route of Administration: Gavage Exposure Period: Days 6-15 of Gestation Exposure Levels: Method: 0, 100 mg/kg aInmamoprneiteustm,p2ernfolnu-oprroeogcntaanntoafteembaylegraavtasgweerate 1a5d0mimngi/sktge/redday or c1o0u0ldmgt/oklegr/adteayfotro tdheetpelramninneedtheexpmoasuxriempuemridoodsoefth1a0tdtahyes.dams For day tthhaetdsepfienrimtaivteozsotuadwy,erfeemdaelteecrtaetds iwnervaegminaatledlatvoamgaelweas,s and the designated as Day |ofgestation (Day 1G). `FcoornsEuxmppetriiomnendturIi,nbgogdeystwaetiigohntsw,ercelirnieccaolrdseidg.ns,Thaneddfaemesd were cweordeedcoflrloecmtjeuds,tabnedfournetislaaclrlifsitcreucutnutriallaallltmeartateironnaslnaontdedfeatmalodnagtathe fetuses were classified. wAfetreeresxaacrmiifnieced,oflitvheer dwaeimgshtonwDasayre2c1oGrd,edg,roasnsdpartephrooldougcitcicvheasntgaetuss s`iwtaess dweetreermcionuendt.edT,haendnutmhebenrumobfecrorapnodrapolsuitteiaoannodfiamllpllainvet,atdieoand, awnedigrheesdorabnedd sfeextuesdesexwteermealrleycoardnedd.intAelrnlallliyv,e aanndd tdheeadlivfeetfuesteussewsere fweetrueseesoxafmeiancehdlfiottrerexttheartnwaleraeltaerlaitvieonwsh. enAprpermooxviemdatferloymYthoefdtahem were examined for malformed fetuses wveisrceersailmiallatrelrayteioxnasm.inIend.addTithieonh,eaaldlsstoufnatleld or tfheeturseemsaeixnadmeirnteodtoftoarlv2i/s3coerfaelaaclhterlaittieornwsearnedfaixseudffaincidenetxanmuimnbeedr of wfoerrehefaidxeadl,tewreartieonesx.amAillnefdetfuoserss,keelxecteapltatltheerahteiaodnss of those that sFaormeExapsefroirmEexnpteIrIi, mtehnetprI,oceexdcuerpetsthuastedboudntyilweDiagyht2s1wGerweerceoltlheected aonndditfhfeeriednetntgietsytoaftieoancdhaoyfsf,sfpereidngcwointshuimnptliitotenrswwaassnnoottmreeatsauinreedd., hAotulseeadstitnwaopdoalyyscabrebfoonraeteexcpaegcetewditpharbteurdidtiinogn., eTahceh ddaatme owfas pwaeritgurhietdioannwdaesxanomtiende,danfodr wcalisnitcealrmseidgnsD.ayFo|rPcPa.chThteestdgarmosupwere lfietrtteirlivtiyabiinldietxy iannddegxeastnadtiloacntaitnidoenx iwnedreex cwaelrceulcaatlecdu,laatnedd.forAlclacdhams were sacrificed on Day 23 PP, without pathological examination. `eTxhaempiunpesd ffroromexetaecrhnadlaamltewrearteioncsoutnotweadr,dstehxeede,ndweoifghDeady, a1 nPdP. `Thereafter, each clinical signs on pup Day was weighed 4, 7, 14, and 2a2ndPPi.nspTehceteedyefsoorfatdvheerspeups in Dbaotyhsg2r7ouapnsdw3e1rePPe.xaTmhiinseedxabmyiannatoipohnthwaalsmocloongdiuscttebdetwwietehnthe tehxepoesyuersewleerveelfsicxeoddefdo.r hOisntoDlaogyic35evPaPl,uaetaiconh.pup was sacrificed and GLP: `Test Substance: Results: Data Yes were analyzed by appropriate statistical methods. IAnmtmhoe nprietuemst,peornfeluroarto(owcteaingohaitneg, purit>y 95% 278 g) dosed at 150 mg/kg showed severe on the mcloirnnicianlgsoifgntsheof5TMtoxdiacyi,tybybywhtihech4"tidmaeyitanhdadwalosstfound dead approximate4l0y g body weight. Another rat (weighing 260g) 5dodsaeyds aotf1d5o0simngg/aktg1l0o0stmagp/pkrgo,xiamdavteerlsye c1l1ingicbayl sthiegn3s wedarye. noAtfter noted in otherrat t1 hraatt ltohsattalpopsrtoaxpipmraotxeilmyat1e4lyg. 6 Og,natnhdis wbearsies,mitnhiemal in the t1h0e0dmagm/skgc/odualyd tdoolseargateelfeovrelthweapsljaundngeeddextopobseurtheepmearixoidmoufm10thdaatys. pInerEfxlpueorroiomcetnatnsoaTtaenwdeIrIe, 5fooufntdhede3a7ddaanmds1gwivaesnsaacmrmifoinceiduimn a tmhoerdiobsuinndgspteatrei,oda,saclolmbputar1eodf1t0h0e dofamthset3h7atcsounbtsroelquaennitmallys.dieDduhraidng cwhertopmeordianecarlyoarrreahseaanadndwecrherolmeotrhharigniocr.rhTewa.o aAlmsoonhagdthe remaining Idnamtshe, c4ondtervoellgorpoeudp,altohpeecoinal,y1clhiandiclalunsgignnoinsoet,eadnwda1shfaodcadliaarlrohpeeac.ia that developed in | dam. aFprpormoxDiamyaste6l-y151/G3,ltehses ttrheaantetdhegrcoonutproiln gErxopuep,riamnedndIturgianignetdhe apomsmtotrneiautmmepnetrfpleuroiroodoc(tDaanyosat1e6--tr2e1aGt)e,dtghreobuopdsyigwneiifgihcatntglayin of the bexocdeyedweeidgthhtatwaofstnhoetctoankterno,ltghreoruepf.oreI,nDEaxypser6i-m1e5ntboIdT,ytwheeiDgahty g1a6inGs were not calculated. tFheeeddocsoinnsgupmeprtiiodo,n twhae strmeeaatesdurgerdouopnlcyonfsoruEmxepdersiigmneinfitcaI.ntlDyulreisnsg feed than the control value control group. Feed in the post-exposure consumption period. was similar to the tMheeadnifmfaetreerncnealwalisvenrowtesitgathitstfiocraltlhyestigrneiaftiecdangtr,ouAptwsaascriifniccree,as|oedf,tbhuet ai 0 Reference: Miscellaneous Type: Species/strain: Method: sdeavmersalgirveednaaremamsoonnituhme vpiesrcfelruaolrsouorcftaacneoaotfetwhaesmeodbisaernvleodbetoofhatvhee liver. rIensEorxppteiroinsm,enatndI, ftehtealmbaoidnytewneaingchetowfeprreegnnotanacdyv,ertsheeliynacfifdeecntceedobfy `Exapmemroinmieunmt pIIe,rfnlouoardovoecrtsaeneofafteectadomninriesptrroadtuicotni.veSipmeirlfarolrym,anince or on pup viability or growth was demonstrated. `cTohmepoonulnyd-ermeblraytoe-dfewtaals taonxiicnictryefaisneddiinngcniodteendcethoaftfceotuulsedsbweith ionsstihfeiccaotnitornoslitgersouopn. thTehifsirsdtiflfuemrbeanrceveinrtienbcriadeevnecreswuas sthsetaitnisctiidceanllcye psrigonbiafbilcyanat ornelsypiofnasenatloygzeenderbayliazeodnes-ttraeislseedvtoekste.dIbtsyptrheesetonxciecwsataste doefvtehleodpammesn.t Tofhethpeoosftfpsaprrtiungm fviraobmilaidtyd,itgiroonawlthdaramtse,gainvden ammonium perfluorooctanoate were not affected. Irnevveiavloedexnaomicnoamtpioounndo-frpeulpast'edeyaelstebraettiwoenseninDathyesp2up7s.and 31 PP hAamzmarodntioutmhepceornfcleupotruoso.ctanoate did not demonstrate a unique DuPont Co. No. 1-82. (1982). Unpublished Data, Haskell Laboratory Report Metabolism Female Female aalnbdinmoalraetsra(t7s-/8Cwrele:CkDs old and wei ghin g a ppr oxi ma tely 220000g2));; apnredgmnaanlteprratismi(garpapvriodxaimfaetmeallyes8 (wweeeikgshionlgd aapnpdrowxeiimgahtienlgy approximately 250 g) were allowed food and water ad used. The libitum. rats were housed 2/cage and GlervoeulpssinAf-eEmarlatesrwatesreastaesftuendcttoiomneoafspuorset-belxoopdosourrgeantoifmleuofroildleowing oGrraoluapdmAin(i2s1trfaetmiaonlearnadtst)o wiansvesdtoisgeadtewmiutlht2ip5lme gv/ekrsguosraslilnyglaenddoses. 3GrroatuspsweBreansdacCrif(i1c2edfeatma%l,e %r,at1s,/2gr,o4u,p)8,waenrde 2o4rahlolyurdsosaeftderwidtoshing. 2a.n5do2r41h5o0urmsg/akftge,rrdeosspiencgt.iveGlryo,uapndD3(2r1atsfewmearleesraactrsi)fwicaesddaots4e,d2, 8, GLP: Test Substance: Results: 11 Y4, days with , 2,4, 8, 25 mg/kg orally each day and 3 24, and 168 hours after the 11 rats were sacrificed dose. Group E. at (12 male rats) was dosed at 25 sacrificed at 5, 8, 24, and 168 mg/kg orally and 3 hours after dosing. rats were iGnrofuempaslFe-rIatrsatfsowlelroewitnegstiendhatloamtieoansuexrpeosbulroeo.d oGrrgoaunopfFlu(o2r4idfeelmeavleels 3ratrsa)tswwaesreexspaocsriefdicteodaasti%n,gl%e,6-1,ho2u,r4,ex8p,o2s4u,raenodf 11608mhgo/umrs' aafntder exposure. Groups G and H (12 female rats/group) were exposed to aansdin3glreat6s-wheorure esxacproisfuirceedoaft 1%,mg2,/m8,'aonrd0.214 mhgo/umr's,afrteesrpeecxtpiovseulrye,. `Group I(12 male rats) was exposed 10mg/m" and 3 rats were sacrificed to at a single 14, 2, 8, 6-hour and 24 exposure of hours after exposure. pGrreogunpasntJ-vNerwseusrenotne-stperdegtnoacnotmrpaatrs eanbdlotoodcoormgpaanroeflouroarliedexploesvuelrse in avdemrisnuissitnehraeldat2i5onmegx/pkosguorrea.llyGroonugpesJt(at1i2onprdeagyna1nSt afnedma3lerartastsw)erweas (s6acprriefigcneadntatf%e,ma2l,e8,raatsn)dw2a4shaodumrisnaifstteerrdeods2in5gm.g/GkrgouopraKlly on gestation days 6-11 and 3 rats were sacrificed at % and 2 hours aadftmeirnithsete6r"eddo2s5e.mgG/rkoguporaLll(y12onprgeegsntaantitofnedmaaylse6r-at1s5) awnads3 rats Mwer(e6 psarcerginfainctedfaetm%a,le2,ra8t,s)awnads2e4xhpoousresdavfitearatshieng1l0e"6d-ohseo.urGroup winehraelastaicornifeixcpeodsautre to 10 mg/m" and 2 hours aofntegreesxtpatoisounred.ayGr1o5uapndN3 rats (3 pregnant female rats) was exposed via inhalation, 6 hours/day, to 10 mg/m' on gestation days 6-15 and 3 rats were sacrificed at hour after the 10TM exposure. Blood No samples were obtained from cach rat `ATmhemuopntiakuemapnedrfcllueoarroaonccteanoofaatem,mpounriituymappperrfolxuiomraotoecltyan1o0a0te%from tthhee pbelaokodroeafcfheemdal1e-2rahtosufroslplooswti-ntgreaastimnegnlteaonrdalwdiotshevwiratsualratpoitdalwith aclpepaarraennctecbhyan2g4eshoiunrsb.looAd doorsgea-nroefslpuoonrsiedewlaesvedlesmofonlsltorwaitnegd mwuiltthipnloe oral dosing. following a A slower single oral clearance dose. rate in male rats was demonstrated wAitshiinngle1 h6o-uhrouarftienrhcaelsastaitoinoenxopfoseuxrpoesruerseu.lteTdheintpeestaksubblsotoadncleevels Reference: Type: Species/strain: Method: GLP: `Test Substance: Results: rnaoptiadflfyecctlebalroeoddflreovemlst.heMballooedr,atasndcltehaerendutmhbeecroomfpeoxuponsdurmeuschdid more slowly. Pblroeogdnalnetvealnsdfnololno-wpirneggneainthterraotsraslhoorwiendhasliamtiiloanreoxrpgoasnuorfelsu.oride `sTuhaeigahmtocuhnationfiasmommeorniincurmeapseersfrleuloartoiovcettaontohaetenopnr-essternatigahsttchehain aidsmoimneirsstraasttihoen tiincmreeafsoels.lowTihnigsasmumggoensiteudmtpheatrftlhueornoono-csttarnaoiagthet chain cihsaoimnerissoamreerc,loerartehdatfarommettahbeolbiltoeodismporreesernatpidly than the straight NDou.Po5n9t3-C8o1. (1981). Unpublished Data, Haskell Laboratory Report Metabolism MMaalleermaitcse/Crl:CD sa1doCullu-ttAimomanmlwoeanrsaitausdmamnipndeir6sftlaeudrouelrdtoobmcyatlaiennotmaratigecaes(t0ar.ti5caWidCnoits/uebmagot)fioa1ns0tamong8/akyqgou.ueonOugnse c1ocnotnrtorlolgrgoruopup(2(r3atrastasnadnd2 m2imciec)e)wawsassascarcirfiifciecdeadta2t496hohuorusrsa.ndThe t(r1e0a0t0edmggr/okugp) (234rhatosurasndaf2temridcoes)inwgaswidtohsethdewtietsthscuhboslteasntcyeraamnidnethen sactificed at 96 hours. After dosing, rats and mice were placed in iatnd2i4v,id4u8a,l7g2l,asasndme9t6abhooulriss.m Fcehcaemsbearnsd. urEixnhealweedre'cCollweacstesda.mpAlted sacrifice time, approximately 81-m2 LmLofobflboloodowdawsasrermeomvoevdedfrformomthethreatmsi.ceLiavnerds owxeirdeizreedmoavneddc,ouhnotmeodg.enized, weighed, and 0.5 g samples were No AAlmtmhooungihuamprpeervfilouuosrosotcutdayno(aDtueP,opnutriRteypaoprptrNoox.im8a2t8e-l8y11-00de%scribed bleetlhoalw)efifnedcitcsaotfedatmhmatocnhioulmespteyrrfalmuionreoorcestiannocaotueldinrtehdiuscsetutdhye,atchuetree wpearsflnuoorsoiogcntoafneoantheanvicaedfeceelsi,miunriantei,onorofex"h*aCl-eadmaimr.onTihuem differences babestowrepetniotnheof2tshteudtiesetssaurbestmaonscet.likIenlythbeepcraeuvsieouosf sdtiufdfye,remncuecshionf the dchoosleewstaysrapmrionbeabwlayssialdlmiinnisttheergeads.troTihnteesntoinn-alabtsroacrtbewdheanmmonium rpeermfolvueordoboecftoarneoaatbesotrhpetniaosnscoociualtdedocwciutrh, cahnodletshtusyrparmeivneen,tweadsthe Reference: Type: Species/strain: Method: GLP: acute lethal effects of ammonium perfluorooctanoate. For both followed rats and by fecal mice, the primary and expired air. route of excretion was urinary, After 96 hours, fecal elimination was nearly the same for both tsahipraenctihteahsne; thrhaotew.rea`vtT.ehriMs,odtsihftefemnrooetuansbcleeyi,enxtpuhirerienmadorusysiegexnicefrxiecctraienottnleydwamlsoesrsreeifn"le"tcChteiendurtaihsneea greater DuPont concentration in mouse liver, Co. (1982). Unpublished Data, Haskell Laboratory Report No. 405-82. Pastor, T. P. etal. (1983). The Toxicologist, 3:82. Metabolism MMaallee aanndd ffeemmaallee rmaitcse MMaallee aanndd ffeemmaallee hraabmbsittesrs A male and female of each species received a single 10 mg/kg dose oTmfehteCa-braoatlsm,imsmmiocnuen,iituasnmdimphmearemfdslituaeotrresoloywcetaraftneeorahtodeoussvieinadg.iinntdErixavpgiaidsruteardilclCyiOnistn,ugbluaartsiinsoen,. daonsdinfge.cesThweermealceollaencdtefdematal1e2,ra2b4b,it4s8,we72r,e 9i6n,diavniddu1a2ll0yhhoouursseadftienr satnadinfleecsessswteeerlemceotlalbeoctleidsm24c,ag48e,s 7i2m,m9e6d,ia1t2e0l,y1a4f4t,eradnodsi1n6g.8 Urine hours affrtoemr dtohseirnagb.biEtxsp1i6r8edhoCuOrys wafatserndootsiconlgl.ected. Blood was drawn dTohseinragtsa,nmdibcle,ooadndwahsamdsrtaewrns.weAlrleaanllimsaalcrsifwiecreed d1i2s0sehcotuerdswaiftther fkiodlnleoywsi,ngsptliesesnu,esteesxtceisseodr,ovwaeriigehse,db,raainnd, Gf.rozI.entr:achte,aratn,dlumnugssc,leli,ver, sfrkoizne,n.andMeftatabsaomlpilsesm.uniTthsewcearrecawsassehsewderaendthtehne wcaegieghweadshaensd were collected and refrigerated. . He rUardiinoea,ctCiOvi;tysaumspilnegs,a lainqduicdasgceinwtailslhaetisonwecoruentaenra.lyBzleododdi,refcetcleys,for tissues, organs, and homogenized carcass samples were analyzed lfioqru"id*Csccionnttielnlattiboyn tciosusnuteero.xidRaatbiboint ucsairncgasasetsiswsueereoxniodtizaenralaynzded for HC content No Test Substance: Results: Reference: SAumbsmtoanntiiaulmsepexrdfilfufoerroeonccteasnoiantrea,tspuarnidtyh7am0s%ters were observed in tfheemaelxecrreattiaonndofth"e"mCaalcetihviatmysftoelrleoxwcirnegteadsoivneglre9o9ra%l dose. of the The original YC activity by 120 hours after dosing, conversely the male rat and tachteivfietmy,alreeshpaemctsitveerlye,xcbryet1e2d03h9ouarnsdp6os0t%-odfostihnge. orBiogitnhalse"x"eCs of fraebmbailtes reaxtcarentdetdhtehmea"leC haacmtisvtietry. asThraepimdallyeaannddcfoemmpalleetemliycaes the epoxsctr-edtoesdinogn.lyT2h1e%raopfidtheexcorriegtionrasl "C activity (female rat, by 120 hours male hamster, and omraglaensanadndfetmiaslsueesraabtbistascr)ifciocnet.aiTnehde nselgolwigeixbclreetaomrosuenxthsiboifte"dCthien lheivgehlesstin"t*hCeckoindcneenytsr,atliuonngss,inanthdesbkilno.od and liver with substantial DuPont Co. No. 62-82. (1982). Unpublished Data, Haskell Laboratory Report Type: Species/strain: PPlraecgennatnatlraTtrsa/sntsrfaienrnot specified Sex/Number: ~~ Females/ Method: cCa-rlbaobneyllepdosaitmimononaindumhapderafslpueocriofoicctaanctoiavtietywoafs0l.a5beulCeidmogn. thWeater was used as the dosing vehicle. The pregnant female rats received a single 10 mg/kg dose of "C-labeled ammonium pweerrfeluionrdoivoicdtuaanlolayteplbaycegdavianggelaosns tmheeta1b9oldiasym ounfigtesstiamtmieond.iatTehleyrats after dosing. Tafwteor droastsinwge.reBslaocordifwicaesddatraewanchattismaceriinftiecrevaalndofre2f,ri4g,eraantded.8 hTouhres dpilsasceencttaesd,furmobmileiaccahl octohredrs,,aannddwfeeitguhseesd.weTrheethuemnbirleimcoalvecdo,rds and aplnadcefnettaussewsewreereinfdriovziednu.allOyrgpalnacseadnidnttoispsaupeesrwceormebaulsstoieoxncicsoende,s wUreiingeheadn,dafnedcaflroszaemn.pleTsheexccarrectaesdsbesetwweereen adlossoiwnegiagnhdedsaacrnidfifcreozen wsuecrceescsoilvleelcytewdiathnddiflruotzeend.eteTrhgeenmt,etwaabtoerl,isamndunaictestwoneer.e washed The washes were collected and stored. `enTthiereptlya.ceSntaamsp,luemsboiflictahle mcoartdesm,aalndtisfseuteuss,escawrecrases,oxainddizfeedceisn wtheerier aclosuontoixnigd.izTehdeanurdinaenaalnydzecdagfeorw'a*sCheacstiwveirtye baynalliyqzuiedd sdciirnetcitlllyatbiyon liquid scintillation counting, UF GLP: Test Substance: Results: Reference: No Ammonium perfluorooctanoate, purity approximately 70% wPlaascesnhtoalwntrtaonsofcecruorfa"fCte-rlaabdmeilneidstarmamtioonnoifuma pseirnfgllueoorroaolctdaonsoeaotfe fCe-tallalbeevlelesdoafmammomnoinuimupmerpfelrufolruooorcotoacntoaantoe.ateTahte.2caonmdpa4rihosuornsof rpellaacteinvtea,to anthdeoctohnecreontrrgaatnisonrsevoebasleerdveevdidinentchee omfatreersniasltabnlcoeotdo, pfeltaaclenctoanlcetnrtarnastfieronosfotfheatmemstocnoimupmoupnedr.fluHoorwooecvtearn,oabtye 4inhcoruerass,edthe csounbtsrtaasnttitaolloythmeorrteistshuaens aelxlaomtihneerdo,rgaamnmsoannidutmispsueersfleuxoarmoioncetda.noaItne ainmtmhoenfietuumsepsedrifdlunoortoodcetcarneoaasteebceotnwceeentnr4a-ti8ohnosurwse.reTshiemipleaarkinfetal magnitude Significant tqouatnhteitlieevseolsfoabmsemrovneiduinm tpheerfspllueoerno,ochteaarnto,atleuncgasn,, and fat. ptlhaecreefnotrael,bbaerrtierranosfffeerrriendgfmrionmitmhaelprleasciesnttaancteottohetrfaentsufserw.ith the DuPont Co. No. 61-82. (1982). Unpublished Data, Haskell Laboratory Report Type: MSeptehcioeds/:strain: PEeffrefcltusoorfoDocotwaenoxateIoinn Exchange Rats on the Toxicity of Ammonium MAalreanrgaet-sf/iCndriln:gCDstudy was conducted prior to the test to find the sttouldeyr,atreadtsdowseereofdDosoewdefxrom1-2X020--C1100(0chmogl/ekset,yra1 mrianve)d.oseInletvhealt, and 6 rats dosed at 1000 mg/kg. noted in this study. No clinical signsof toxicity were GLP: Test Substance: Results: Aadmmminoinsiteurmedpbeyrfilnutorraogoacsttraincoaitnet,ubaastiaosnutsopeynosuinogn aidnuclotrmnaoliel,rwatass Four groups, 6 dosed with 500 rats/group, were used mg/kg of ammonium in the study. Group perfluorooctanoate, I was Group If was dosed with 500 mg/ke of ammonium perfluorooctanoate and immediately dosed IT was dosed with with 1000 1000 mg/kg of Dowex mg/kg Dowex 1-X2-C1 1-X2-C1, Group and 2 hours later dosed Group with 500 mg/kg of IV was dosed with ammonium 500 mg/kg perfluorooctanoate, of ammonium and perfluorooctanoate and 2 hours later dosed with 1000 mg/kg of D14odwaeyx"rec1-oXv2e-rCy1.p.eriAoldl rats and wtheerneswaceriigfhiceedd.and observed over a No Ammonium perfluorooctanoate, purity approximately 100% PRrees-idnoastin1g00or0 pmogs/tk-gdocshiangngweisththDeotwoxeixcef1fe-cXt2s-oCfIaTmomnoEnxicuhamnge Reference: Type: Method: GLP: `Test Substance: Results: Reference: perfluorooctanoate in rats. All rats dosed with the pDeorwfleuxo"cc1ta-nXo2a-tCel,haeditrheedrubceefdormeorotralaifttieerstcheomapmamroedmitoumthe rats wdeorseed5/w6i,t0h/a6,m1m/o6,mainudm0/p6erffolruGorocotuapnsoaIt,eILalIoInIe,.anMdorItVa,lity ratios respectively. wAasfoplelrofw-ourpmesdtutdoyd(etDeurPmoinnteRwehpeotrhteNroc.ho4l0e5s-t8y2ra--midneescwroibuelddabove) efnrhoamntcheetbhoedyel.imination of absorbed ammonium perfluorooctanoate DuPont Co. No. 828-81. (1981). Unpublished Data, Haskell Laboratory Report `RTehseppiurraptoosreEwvaaslutaoteivoanluateM.S. A. Combination Type GMA-H brersepaikrtahtroorugcha.rtrTidhgeescaargtariindsgteapmaimrosnwieurem tpeesrtfelduaograoioncsttanaonataevedruasgtefor gcoennceernattreadtiinonaofd0us.t5g6enmegra/tmo'r,aamnmdoinnitruomdupceerdfliuntooroaonctaainrobaltoew of t6e0mpLeirmaitnurmea.inBtoatihneudpsattr5e0a%m arneldatdiovwenhsutmriediatmyaiarnbdoarnmebient room dcounsctebnytrfaitltieornpsawpeerrecamsosneittteosrebdacfkoredamupmowintihuamnpiemrpfilunogreoroscatamnpolaitneg Atrlaiiqnu(o0tsabosfotrhbepaoqsuseiobluesaexmtmroacntisuomf tpheerffillutoerropoacptearnoaantde avlaipqourost,s of the aqueous ammonium piemrpfilunogreorocstaamnpoaltees, were analyzed colorimetrically for No AAfmtmero4n0iuhomurpserofflucoornotoicntuaonuosateex,popsuurritey, not no specified detectable amount of daomwmnosntireuammpoenrftlhueorboroecattahnionagtseiddeusotfotrhevacpaortrrwidagse found pairs tested. The pmeirnfliumouromocdteatneocattaeb.leBlaimsietdwoansa1.452u0g Lofaiar msammopnlei,utmhis would calculate to be concentrations 0w.e0r0e40m.g6/7m%'.0.3T3heanmde0a.n48up=s0t.r1e5ammgai/rmb'orante 40 and c5o4mbhoiunrast,iroenspteycpteivGelMyA. -THhecarretsruildtgsedseemfofnescttrivaetleydftilhtaetrtahmemMo.Sn.iSu.m cpoenrdfiltuioornosocftoarnoaamtiendiusmtuamndofv4a0pohroucrosn.centrations at the test NDou.Po6n64t-8Co0.. (1980). Unpublished Data, Haskell Laboratory Report Type: Method: GLP: Test Substance: Results: Reference: Type: FGilvoevetyPpeesrmoefagtlioovensT(ensetoipnrgene; neo-synthetic latex rubber, floc plienremde;altaitoenx;rneastiusrtaalncleattoex;a n3e0o%praeqnueerouubsbesro)luwteirone oefvaalmumaotendifuormtheir Spaermfpllueosrooofcttahneogaltoevaesndwearmemtoensiteudminpedrufplluiocratoeocitnan1o0amteLdrgylapsoswder. `inpseirdmeeastuirofnacceelolsf wthiethglwoavteesr. uAsed10asmaLcaollilqeucottioonfmtehedicoulmleocntitnhge smpeedcituromphwoatsomaentarlyyzaefdtefroarna8m-mhoounrieuxmpopseurrfel.uoBroroecatkatnhoraotuegbhywas pdeertfelrumoirnoeodctwahnoeantea wdeatsecftoaubnlde ainmothuenctololfeactmimononmieduimum, The `1 mpign/immuLmindettheecctoilolnelcitmiiotnfmoerdaimumm.onium perfluorooctanoate was No RAemsumlotns iofumthepeervfalluuoartoioocntasnhooatwe,thpautr3itoyfntohtesgpleocvifeiseadmples d(noehoapvreenae;menaeos-usryanbtlheetbircealakttehrrrouubgbhert,ifmleocanldinpeed;rmaenadtinoatnurraatletaeftxe)r p8ehrofluurosorofocctoanntoiatneu.ousHoewxepvoesurr,et(h0e ape3r0m%eatsiolountiroantoesfaaremvmeornyiluowm. indicating substance. that the materials do offer some resistance to the test Rpeoswudletrs osfhothwettheasltsinpe4roffortmheedsawmiptlheasm(mnoeonpiruenme;penrefol-usoyrnotohcettaincoate lbarteeaxkirubbrboeurg,hfwlaocsloibnesde;rvlaetdexa;ftaenrdnheoouprrseonfecrounbtbienr)uonuos exposure, DanudPoonntlyCoa.ve(r1y98s1m)a.llUanpmuobulnitshpeedrmDeaattae,dHathsekellalteLxasbaomrpalteo.ry Report No. 612.81 `BSiuompemrasriiszteedncinetShcerPeeFnOiSngseScttuiodny, see DuPont (2000). Haskell Laboratory Report No. 2922. 20 SUMMARPYEORFFLSTUUODRIOENSOCNOANNDOUACTTEEADTWDIUTPHOANMTMONIUM Mammalian Toxicity Acute Toxicity Type: VSpaelcuiee:s/strain: Method: GLP: Test Substance: Results: Reference: Oral ALD M1a87lemgr/atksg/ChR-CD. dITonhstereasgt.easstCtrosinuccbesintntatrnucabaett,iioaonsnsattoessoytloeuudtniwogenraiednul1wt.a0tm,ear1l,.e5w,ra2ast3s,a(d31m./4ig,nri5os.ut1p,e)r7ei.dn7,sbi1yn3g,le w21e27i,5g02h6tm,sg4/0ak,ned.60l,iSvu9er0rv,wie1vi3og0r,hst1sw8e7wr,eer3es0a0rc,erci4of5ri0dc,eedd6.7104,d1a0y0s0l,at1e5r,00a,ndanbdody No MDAoemratmtaholsnitoiycucwmuarsrpeeordbfswleiutrohvrieonndo6antadncaooyanstceea,fnttperurartdiiotosynisnngoo.tfsS1ple8ic7gihfmtigei/dnkitgiaalnwdeiagbhotve. sT1io2g,snsasenswdoecr1ce5urodrbaesydesraavtte26d60,bae9nl0d.ow4a0n2d6mgm1/g3k/0gk.mgg./WkeCgil,ginhritecsaplleocsstsiigovnecslcyuo.rbrseNedrovfecodlri9na,itcal ac2hn6edwmaigbn/ogk-vgmeoaitnnidcolnausbd.oevdeAbdeidlnilctyli-uocdnreaadlwlpcialnligln,oirc,haalslasfli-igcvnlasotsiooebnds,eepyroevlse,yduraita,18a7ndmg/kg liinvceororwdeiingahttisona,nrdufifnlcerdeafsuer,d dliiavrerrh/ebao,daynwdeeimgahntcriaattiioosno.ccIunrcrreedasated 3.4 mg/kg and above, DNou.Po1n2t9-C6o8.. (1968). Unpublished Data, Haskell Laboratory Report Type: Species/strain: MeVatlhuoe:d: Inhalation ALC Male rats/Crl:CD(SD)BR 2M59a98l0egm,rgaw/tesmr'(e6/egxrpooups)e,d 8viwaeneoksse-oodnlaynidnhwaeliagthioinngfobreatwsienegnle2,344-haonudr apierr. ioCdon1c0eantdruasttiaontsmotsepshteedreweorfea6m2m0,on9i10u,m 1p6e0r0f,launordo4n6on0a0nmoga/tme 'i.n ivDnoutlsoutmaeagttlrmaioscsspfthereeardnescfsoenrwtertrobleel.egre.AneiTrrhaitenetdbriwonidftuecheeaddebaritnmtehfteeeetrdueebdretrseeswgteulpsauttbestdhteawnticetseht a wcshauabsmsbgteearnn.ecreaFtthoerrdotbuhgyehplaaoswsseiisznteg-erpxerpdeouscssiuunrrgeizcceyodcnlcaioernntetrhaartnoiduognih,ntatohgtelhaaestsemxogpesonpsehurreaert.eor. A flask at the botiom of the generator served as a dust reservoir. A Il GLP: ReTseustltSsu:bstance: bsetoyitertlrioonnmgoe rfeoltduhteargiiartteaostreerwdvaodsiursitannsidenrtattheedthageebnocevyrcealttoohnre.e reeAlsiuetrrrviioanittror.rosdAuwcemepodttaodtrutishtzee. d icpnoatnrectrievcnaltlerssabtiniytoodnrstahwewaiesnxgpdoecstauleirrbemriacntheeaddmbavetorla.uppmreToshxoeifmaatctmheoalsmypbh3ee0rrimacitnmuotsephere dstaehmtrpeolruimgnihgn.feidltCefrhsr.aommbTtehhree tafetimmlpeoersrpwaheteiurgrihec,tcrdoienflfcaeterinevtnertaihtaulimoibndeifotofyr,peaarantniddcualfattoerwas waXtyegrenwecroentaevnat iwlearebalmdeealsiubirteudm.. Except during exposure,cfhoaomdbearnd Body weights and clinical signs were recorded. Survivors were sacrificed 14 days later iTvoerm,on2igtroorupthseoefff1e0ctrsaotsf, a8mwmeoenkis uomld paenrdflwueoirgohnionnganboeattweeoenn t2h3e7 Tanwdo2g7r7oug,psweorfe10exaptoss,ed Were exposed to air only. wteoe6k7saonldd 5a9n0dwmegi/gmh'i,rnegsp2e3c1tiavnedly3,67 Each control group was exposed g, tschaoecnrcliuifvriercree.ndt5lyorwi1t2hdoanyesoaffttehreetxepstosguroruepsf.or pFaitvheolraotgsi/cgeroxuapmiwnearteion of Yes hCAuhmmaimmdoibtneiireustmermappneegrrefadltuuforrreoosmnro1ana9nn-go4ead5t%eb,,eatpnwuedriectnyh2a33m9-b29e76rCo,xyrgeelanticvoente were 21%. nts MOonretoalfitthyerartaitossionft0h/e6,59406,mg6//6m,"adnide6d/o6nwtehree 1ob2sedravyedofienxpthoesu6r2e0., (i96m170m,-e1d16i60a00t0e,mlgya/npmdo's4)t6-0tee0xsptmosgsu/ubmrs'et.ainnCccleliunodinecdatlhreesdihgeonrasdborboswenrvfeadcidaulrdiinsgchorarge 4600 mg/m', labored breathing (4600 mg/m')(,62p0r,of1u6s0e0,claenadr nasal (a4n6d0o0ramlg/dims)c.harNgeosa(d4v6e0r0semgcl/imn'i)ca,l asnidgndsiwmeirniesohbesdesrtvaertdlerinessepsosnse. rceluxifpnfoilcseadeldosritogdni6ss7caotmlghori/egmdh"efrutrch,ornroceudegnhotorruatbtritoohnewsnreifcnaoccivlaueldreyddipsehcruhianodrc.gheesdC, opwomestmtuoorrne, lsitmapinneedssp,erainndeuhami,r pallor, loss, lung noise or labored breathing, lethargy, pRoastts-eexxppoossuerde,0fo6l7lmowge/dmb'ylonsotrm1a-l9%woefiginhittigaalin.bodAyt wceoingcehnttr1adtaiyons ebgirotedhaeytrewrtehtirhgoahuntg6h1o7udmtagyt/hpmeo'sr,te-rceaotxvspeolrsoyustrpeea,rpipaorndodxociromunantttieinlluytehd6e-yt1od5il%eods.oefwMienoiistgithatl 5su4r-v7i1vi%ngofraitnsiteaxlpbosoeddy wtoei5g9h0t, w62h0e,north9e1y0wemrge/ms'acrwiefiigcehded12ondlayys post-exposure or at the end of the recovery period. References: Rats exposed to 67 mg/m" had significantly elevated mean liver weights and liver-to-body weight ratios on the 5 and 12" days afer exposure. Rats exposed to 590 mg/m' had significantly eLlievveartweedilgihvtesr-ftoor-tbhoedsyewreaitgshwterraetinoostosnigtnhiefi1c2an"tdlyaydiafffteerrenetxpforsoumre. the controls on the 5TM day of recovery, and mean liver weights twheersee ssiegenmiifincgalnytliyndceonpsriesstseendt ochnatnhgees12w"erdeayduofe rteocsoevveerrye. bHoodwyever, ewxeaimgihntaltoisosninofrartastseexxppoosseeddtoto559900mmg/gm/'m.' Gross pathologic revealed discolored livers with prominent lobular patterns in 4/5 rats sacrificed on the 5" day of recovery, and similar gross liver lesions in 2/5 rats sDaucProifnitceCdoo.n(1t9he85)1.2UdnapyubolfirsehceodveDrayt.a, Haskell Laboratory Report No. 293.85. Kinney, L. A. etal. (1989). Food Chem. Toxicol. 27:465-468. Repeat Dose Toxicity Type: Species/Strain: ESxepxo/sNuurmeber: Period: Frequency of Treatment: Exposure: Levels: Method: GLP. Test Substance: Repeated Dose Oral Toxicity Study ~~ MMailcee/aCnrdl:fCemDal-e1/(SI/CgRro)uBpR 14 days Ad libitum for 14 days M0,al1e,3a,n1d0,fe3m0,al1e00m,ic3e00(,5-1600w0e,e3k0s0o0l,d)10w,e0r0e0 fmegd/dkigets containing Vaemrmifoincaituimonpoefrftlheuocroonncoennatnroataitoenofofrth14ectoensstescuubtsitvaencdaeysi.n the wpreerpearreecdodrideetds wtehrroeugpheoruftortmheed.testB.odAyftweerisgahctrisfiacned, lcilvienricwaelisgihgtnss `waeprperoprreicaotredesdt.atisBtoicdayl mweetihgohdtss.anSdaclriivfeircweesiogchctusrrweedreonantaesltyzdeady w6itath `ccoonncceennttrraattiioonnss ooff 1100000anpdp3m0a0npdpgmr,eataenrd, oonn tteesstt ddaayy 184atfor the remaining test groups. No Ammonium perfluorononanoate, purity 99% = 53 Results: Reference: Mmoarltealmiitcyerafteidos10o0f,03/50,0,0/15,0000/,5,3030/50,,aanndd 11/05,w0e0r0eppombs,errevsepdecftoirvetlhye. Mortality ratios female mice fed of 0/5, 3/5, 4/5, 100, 300, 1000, 5/5, and 3/5 were observed 3000, and 10,000 ppm, for the rselsipgehctttiovesleyv.erMeiscpeorfaeddiccowneciegnhttraltoisso.nsMoifc1e0f0edppcomncoernltersastieoxnhsiboifted 300 ppm and higher exhibited severe weight loss, ruffled fur, lethargy, low posture, and limpness. Male and female mice fed diets of 3 ppm or higher had sweiiggnhitfsi.canTtlhyeinmceraenasreedlamteivaenliavbesrolwuetieghatnsdomfemaanleremlaitcievefleidvetrhe 1 ppm diet were also significantly heavier than the controls. Comparisons of mean body and liver weights of mice fed diets above 30 ppm were control groups. not possible because there were no concurrent DuPont Co. No. 401-85. (1985). Unpublished Data, Haskell Laboratory Report 53 ol