Document 3Q6XyDabbE152mdrnDk1oYmj0

A R 126-05 23 MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, FC-143 or as the major component of FC-1015 or FC-1015-X. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The test sample is FC-1015-X. It's purity was not sufficiently characterized, though current information indicates is a 30% straight carbon chain version of FC-143 in 80% water. The 3M product lot number was not noted. The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity o f the test sample with that of the test substance. Data was used to compare toxicity of the branched/straight chain ammonium perfluorooctanoate homologue mixture in FC-143 vs. FC-1015-X. METHOD: Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1996 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A primary 6.660 g/L stock solution was prepared in Millipore Milli-QTM water. The pH of the stock solution was 6.9 and an osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL of stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilution. Exposure vessels: 3 mL Cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control 004034 Element basis: Percent light loss. RESULTS Nominal concentrations: Blank control, 416, 832, 1665, and 3330 mg/L Element value: 5-minute EC50 = 2300 (2070 - 2560) mg/L 15-minute EC50 = 1960 (1730 - 2210) mg/L 30-minute EC50 = 1950 (1760 - 2160) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. CONCLUSIONS The FC-1015-X 30-minute EC50 for Photobacterium phosphoreum was determined to be 1950 mg/L with a 95% Confidence Interval of 1760 to 2160 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number P1626. OTHER Last changed: 5/25/00 004035 BESTCOPYAVAllABLE ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET V:."A-- TEST SUBSTANCE: . * Pc. - 10 I S - V ( g - 00 - lq O LR: P/C.2 Date: Analyst: - y y < ; PiJ 5 of 04036  ro 'O O iiw iw m m ooisb BEST COPY AYAHABLE .u c p n r u x ija ta repo r t rii.t PC'*i oi5.r i 5 Ba s i c T e s t PlACA F C - lv lO - X ( W I- S lV D Test Time: Ij minutes Osmo t ic Ati iiistmenfcjNaCl NUMBER Control C:ontrol ;07iT 6/ 9 3 . 0 6 / 74. <1 93.50/ 74.06 ' 7 -ilV COMO. . 0 . 0 0.0 C R / G A M M A 7i-gsj 0.7732 * V .7914 # : f7)6A' ` - L 94 .7 5/ 54.65* 93.57/ 57.24 ' 16 . 2 5 0 0 416.2300 ''0-200' 5 l . <? " .79 -.5 4 94, 9 9 / 5 2 . 8 3 j *. 8 3 2 . 5 0 0 0 90.51/ 52.69 B'52.5000 .^>0.346 ' 5.7.0 a-s.* ' 0.050 5 6 89.21/ 46.00 1665. .<000 8 8 . 4 3 / 4 2 . 0 0 V.0T.V "* 1 6 6 5 . 0 0 0 0 0.519 # *t-`. 3 rJ H 7 86.97/ 8.53 73 SO.Oooo * - V 6 .9 8 8 tt - S9.5 i 8 5 . 9 9 / 1 0 . 5 3 3330.C 000 . 3.39V * C P -- C o C O R R E C T l O N F A C T O R = ' b T ^ 8 `J3 # U s e d iur c a l c u l a t i o n s .7 j C L u O i9 5 7 . 2 0 6 6 MiS/t. 93-/. CONFIDENCE: RAWSE1 / 3 2 . 4 0 7 0 TO 2 2 1 1 .1 7 7 0 ) ` 1tv V-/17 , i>. 1 6C 0^0