Document 3NpR61x87Ra44kE34x28gkVYO
AR 286- 0729
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
IESTSUBSTANCE
0000
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot numberwas not noted. The test sample disieFtCh-y2l0e3n.e gCluycrorlenbtutiynlfeotrhmeart,io3n7.in8d5i%cawtaetseirt,is2a0%mixettuhryeleonfe1g.l3yc4o%l,P2F.O6S6,%35% Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrationsofimpurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:
000000000
Method: OECD Guideline 202
Type: Acute static
GLP: No
Year completed: 1991
Species: Daphnia magna
Supplier: In-house cultures maintained at EnviroSystems Division, Hampton,
NH.
Analytical monitoring: Temperature, pH, conductivity, and dissolved
oxygen.
Exposure period: 48-hours
Test organism age: <24 hrs. old
Statistical method: LCs values calculated using a computer program from
Stephan, 1983.
Test conditions:
Dilution water: Filtered natural well water.
-
Dilution water chemistry:
Conductivity: ~ 700 ymhos/cm
pH:
7.9
Lighting: Cool-white fluorescent lights at 12 uEin/secim?. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by dissolving 2.0 g of test substance in 2000 mL of dilution water. Exposure concentrations were prepared by addition of the appropriate volume of stock solution. Exposure vessels: 250 mL beakers with 200 mL exposure solution and a water depth of approximately 6 cm.
000664
Number of replicates: 4
Numberof Number of
dcoanpchennitdrsatpieornsr:eplfiicvaetpel:us5a
blank
control
Water chemistry during the study:
PH range (048 hours):
7.9-8.3 (control exposure)
8.08.3 (1000 mg/L exposure)
`Temperature range (048 hours):
19.7 -- 20.6 C (control exposure)
19.4 -- 20.6 C (1000 mg/L exposure)
Conductivity range: (0-48 hours)
640- 700 umhos/cm (control exposure)
640 - 660 mhos/cm (1000 mg/L exposure)
Dissolved oxygen range (0-48 hours):
8.3 -8.8 mg/L (control exposure) -
8.0-9.9 mg/L (1000 mg/L exposure)
REswts
000
Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L.
Element values: 48-hour ECs) = >1000 mg/L.
Element values based on nominal concentrations
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadlouenstrheepmoirxtteudreapapslydetosctrhiatbemdixitn utrhee aTnedstnot the fluorochemical proportion alone.
co0 nclusio0 ns 0000
Thihgehetsetstcsonucbesnttarnacteio4n8-tehsotuerd.ECNso; wmoarstadleittyeromrisnuebdlettohablee>ff1e0c0ts0wmegrleL,obthsee.rved. SPauublm,itMtienrn:esot3aM, C5o5m1p33any, Environmental Laboratory, P.O. Box 33331, St. -
pa0 taQuaw0 ry 0000
tReesltiianbgi.litHy:oweKvieirm,istchhersaankmipnlge=pu2.ritTyhiwsasstundoty mpreoeptesrltyhcehcarriatcertiearfiozredquaalnidtythe
study in the
lacks analytical solution.
confirmation
of
the
amount
of
fluorochemical
proportion
REFERENCES
00000
000665
`This test was conducted by Envirosystems Division, of Hampton, NH at the request of the 3M Company,
Resource Analysts, Inc., Lab Request number
H2864-9, 1991
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BEST COPY AVAILABLE
IX. REFERENCES
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P0irn8aDct.thieceA1.5d8s1e.iss(eD3n1e)tcis3oi0fonC(hPoeifnmailct)ha.els.CounAcmimlexCo3n.cerOnEiCoDg PtrhiencNiaptlueasl oAfccGeopotdancLeaboofratDoirtya OKcD. 1984, Guidelines for Testing of Chemicals, Section 3: Etfects on Biotic TSyestt.ens.AdopMteetdhoAdpr2i0l2,4,Da1p9h8n4i,d sp., Acute Immobilization Test and Reproduction CStoempmhuatn,atCi.oEn.. 1983. Computer progeas for calculation of CSO values. Personal
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