Document 3NG9qNx4879r6XnM3nQ8YJJMD
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooetanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-603F. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 501. The testsample isFC603F. Currentinformationindicatesitisa mixtureof0.5% PFOS, 7% diethyleneglycolbutylether,82.95% water,2% acrylifcoamer,4% Alkylether sulfate1,.45% Xanthan gum, 1.4% calciumacetate,0.07% N-(3Chloroallyl)hexaminiucmhloride0,.5% triethanolamine0,.05% sodium hydroxide,0.03% cis-1-(3-Chloroallyi)-3,5,7-triIa-zaaz-oniaadamantane chloridea,nd 0.05% tolytfiazole.
The followingsummary applies to a mixture with incompletely charactedzed concentrations of impudties. Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD
Method: OECD Guideline209 GLP: No Year Completed: 1994 Analyticalmonitoring: Dissolvedoxygen concentrabonst,emperature,and pH. Statisticalmethods: Resultswere determined by calculabonof the % inhibitonin respiratiornate. Test organism source: Activatedsludge mixed liquorcollectedfrom the Metro Wastewater Treatment Plant,St.Paul,MN. Test condition:
Dilutionwater: MilliporMeilli-QTwmater Synthetic sewage: per OECD method Reference and testsolutionpreparation: A stocksolutionofthe referencesubstance,3,5-dichlorophenowla,s prepared by dissolving 500 mg in1OmL 1N NAOH, dilutedto 30mL with MilliporMeilli-QTM water,then brought to the pointof incipienptrecipitatiownith 1N H2SO4 and dilutedto 1L withMilli-Qlwramter. The pH ofthe referencesolution was measured tobe 7.5. Test substance was added directltyothe testvessels. Test vessels: Not given. Number of concentrations: one and a blankcontrol,induplicate, plusI referencesubstance Temperature: 17.7- 20.2 OC Totalsuspended solidsand pH on day of testing:4.1g/L (TSS in final1:.6g/L),initipaHl 7.7and finalpH 7.l.
ElementBasis:Respiratiionnhibitaisodneterminebdyoxygen consumption. Remarks: Values correctedto200C forcalculations.
RESULTS
Nominal concentrations: Blank control,and 1000 rng/L,induplicate. Reference substance at 10 mg/L.
Elementvalues: 30-minuteEC50=>1000mg/L 3-hour EC50 = > 1000 nig/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The FC-603F 3-hourEC50 foractivatedsludgerespiratioinnhibitiownas determinedtobe >1000 mg/L,the highestconcentrationtested.Mean value of 10.1% inhibitioantthisconcentration.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:Jimischranking= 2.This studymeets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportionin solution..
REFERENCES
This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Ml 655, 1994.
OTHER
Last changed: 6/26/00
Type Test t@t@-v-3
ENVIRCNME,4TAL LABCRA-I CRY ACTIVATF--"S)LUDGE MIXED UCUOR
LR
Analyst Page t of
. @@, 11@@ Ir_C-) -., \It\.-k'c C-) -Tt:
ACTIVATER) SLUDGE MIXEP LIQUog
Date Obtained -,-\C.-(k--A
Date Used S-- kC
MixedUquor Suspended Solids(MLSS) '---\\ g/L Adjusted to
MLSS in finaltestsolution (c g/L
InitipaHl -1---A(1st testsoin.prepared)
FinalPH
Comments
g/L (lastsoln.prepared)
TEST SUBSTANCE Sample Description InitiaplH of highestconc. tested %C-C @,,,IL Comments
Adjusted to pH
REFERENCE-SUBSTANCE Sample Description InitiaPlH of stock solution Comments
c- Cmg/L)
Adjusted to pH
EXPOSURE CONDMONS Test Duration
Test Temperature Range:
c
LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIONTEST X209 TEST SOLUTIONS PREPARATION
Test Substance: Sample Description: Analyst: Date:
Test Conc. mg added
mgll
per 500 mL
mL MO(l) water 22r 500 mL
mL syn.sewage feed per 500 mL
mL microbial inoculumper 500 mL
TotalVolume mL
(1) MilliporMeilli-ORhighpuritywa!er
LR
ENVIRONMENTAL
LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: 7-
L c4
Source of ActivatedSludge Mixed Liquor:
Date Received:
'k%-t
Analyst: , - -)-/-1z E
Date Used:
IC@ L I
30-MINUTE EXPOSURE
DL-,SMVED OXYGEN CCNce4TPATIONS, mgtl
TestSubstance Concentration
TIME-MINU ES 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
BK CONTROL I
4
c,
c.
5.4
itemp CIC
&K CVMCX a
C,
3-HOUR EXPOSURE
DLCWLVED OXYGEN CONCB4TPATIONS, mgtl
TestSubstance
TIME -MINUTES
Temp
Concentrabo(nn/i.)0.5 1.0 l.S 2.0 2.5 13.0 3.S 4.C) 4.S 5.0
OC
BK CONTROL 1
T. C'
cA.Lk
ct.
BK CONTROL a
PH Of activated sludge mixc-I '.!qL;or: Initial-11-7 PH of highestconcentratiotnested: Initial--7.@t .Test temperature: -1,-1 - ao.
Final Final -1.1
EMRONMENTAL LABORATORY
Da
OECD ACTIVATED SLUDGE RESPIRATION INHIBMON TEST M
CALCULATION OF RESULTS
Test Substance: FC 603F (Lot-#501)
Analyst:
30-Min to Exposure mq/L 0.5 i.o 1.5 2.0
'gKl 6.4 6.1 5.7 5.4
looo.o 4.1 3.5 Dup. 5.9- 5.4
3.1 1 2.7 5.0 4.7
DissolvedOxy an Concentrati s.mq/L 2.5 3.0 3.5 4.0 4.5 5.0 5.5
5.1 P44.070 4.3 4.0 3.6 3.2 2.9
2.3 2.0 1.6 1.3 0.9 0.6 4.3- 3.9 -3.5 3.1 2.8 @22C .5 2.1
TempI.C
-R (1) Ra
20.1 0.7162 0.71
20.2 19.8
0.7367 0.72 0.7358 0.74
BK2
8.0 7.7 7.4 7.1 6.8 F-6-s 6.1 5.9 5.6 t 5.3 5.0
% diffincontrol(s4): 0.7
ECSO (95% Cl):> 1000 m
17.7 0.6012 0.70 RaCm
3-Hour Exposure
DissolvedOxvcan CornicentratSi.matl
ma/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 T-1p@'c R (1) Ra
BK1 7.4 7.1 6.8 6.6 6.3 5.8 5.6 5.3 4.9 4.7 4.3 18.8 0.6255 0.67
1000.0 6." 6.0 5.7 5.3 5.0 4.7 4.4 4.0 3.7 3.4 3.0 Dup. 7.6 1 7.3 7.1 6.8 6.5 6.3 6.0 5.7 5.5 5.2 4.8
19.5 1 0.6594 1Q.6( 19.0- 0.5455 0.5(
BK2 7.9 7.5 7.3 , 6.9 6.6 6.3 S.9 r 5.-6-T3- 5.0 4.6 118-3 . 0.6497 0.
-rkdififncontro(l4s): 7.3
EC 60 (96% Cl):> 1000 mg&
RaCm
Method: N/A
(1)A. Oxygeunplakreai(emg02A.-ndn)
(2)Ra-R &41uslto2d0*C-tWO-Fxl (3)% inhiMImi-(R&Cm)-RaSI(RaCm)1100
a. 1.072 RaCawmeanadotodR.cortrols Mt-blw*awrolS.- S&mPlO
(4)% dmwoncoinOMrOb:fiRaSkI.RaB$QVRSC(MTIwIoCOMDrOlMsUSI bewkhi1n5%ofeachottw.) Tobs-$Sff4U"MP
(s)meanad@Medfta.mrolsI-ftSki*R&BlQY2
lx.Tot*-20-C
ENVIRNOMENTAL LABORATORY
LR M%FSIC-
OECD ACTIVATED SLUDGE RESPIRATION INHOITION TEST#209 TEST SOLUTIONS PREPARATION
Test Substance:
Sample Description:
Analyst:
Date:
Stock Soluflon
Preparation:
S,\c
C
Test Conc.
mL added per 500 mL
mL MO(l) water mL syn.sewage
per 500 mL
feed 2ar 500 mL
mL mkxobial inoculum = 500 mL
TotalVolume mL
kc)
(1)MilliporMeilli-ORhigh puritywater
%@,S
A LT -1
ENVIRONMENTAL
LABCRATORY
OECD AC,7VATED SLUDGE RESPIRA-IICN :NHIBiriCN 7-mSi-:r2O9
RAW DATA
Test .Substance:
'C@- -%);c
Source of Activated Sludge Mixed Uquor: t%AtN%v
DateReceived:
Analyst:
tC.-Ir@tc
DateUsed:
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRAMCNS,
mgtl
Test Substance Concentration
TIME-MINUTES
Temp
0-5 1.0 1.5 2.0 2.5 3.0 3.S 4.0 4.5 5.0 5.S OC
BK OONTRCL i
Li,
LI.C@
BK CON-MOL I
3-HOUR EXPOSURE
OL",WLVED OXYGEN CONCENTRATKX4.% mgtl
Test Substance
TIME - MINUTES
Concenuadon (-SIL. o.s 1.0 1.5 2-.0 2.5 3.0 3.5 4.0 4.5 5.0 S.S
BK CONTRCL 1
-7.4 -7.1
C.. r., -7,G
6,,l
S@?3 6@(-, i -.3 -7-t 'I.0
Li,C? Li-
Temp cc
SK CONMOL a 7,c
PH Of activated sludge mixed liquorPH Of highestconcentrationtested: Test temperature: 1"1.-1 -
Initial Initial (o c-%
Final Final 7,L,
EMRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRAT10N INHIBMON TEST M CALCULATION OF RESULTS
Test Substance: 3.5- DichlorophenoRleferenceToxicani
I LR:'
Date:
Analyst:JBD/RJW
30-Mi to Exposure
Disso[vd Oxyaen Con entrationsm.
mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp. -C
R (1) _ftaU,!L
BKI 6.4 6.1 5.7 5.4 5.1 4.7 4.3-- 4.0 3.6 3.2 2.9 20.1 0.7152 0.7102
10.0 7.5 7.3 7.0 6.8 6.5 6.3 6.1 5.8 6.6 5.4 5.2 19.3 0.4733 0.4969
BK2 8.0 7.7 7.4 7.1 6.8 6.6 6.1 5.9 5.6 5.3 5.0 % diffincontrols(4): 0.7
3-Hou Exposure
Dissolvd Oxvoen Concentrationsmg,/
mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
BKI 7.4 7.1 6.8 6.6 6.3 5.8 5.6 5.3 4-.g 4.7 4.3
10.0 8.2 1 8.0 7.8 7.6 7.4 7.1 7.0 6.8 6.7 6.5 6.3
l7s7
0.601i .0.7055 RaCm (5):
...p...-. c....R (1) Ra (2) *A 18.8 0.6255 0.6799 19.7 0.3733 0.3812
BK2T7-9
7.5 7.3 6.9 6.6 6.3 .5.9 1 5.66j 5.3 5.0 4.6
% diffincontrols(4): 7.3
18.3 1 0.6497 10.7@il-2. RaCm (5):
(1) R. OrMn uptakerate(mg 02A.-mdn) (2) AS. A adimled to20*C- [We Es) (3) % k*"iww i(PaCm)4ts&(R&Cm)1100 (4) % dgkrwwe in confrob:(lFka8kl.R&Bi2l/RaCm]1(0T0wo coritroMkUM (5) Mean adjuood A, cordrols(-R&Bkl.RaBk2p
be wflhin16% of eachother.)
9- 1.072 R&Coft mean adoatedA. cwmb Dk- blankomrd. S- sample Tobs- sampletomp ix- Tobs-20*C
ChainofCustodyRecord 3M EnvironmentaLlaboratory
Fa- @5"I -PWO
31A Fac -v
V'Vw\ cc
Ad"ss
P,.5;
Cey. S*$-.Zoo, CON
3m Pro@ci 140
"@;,n
3M FACI14YCMISO
Vs fe*ph@ NL*Nwr
01934
sw-pkB"y(ft")
Item
Sample
\@escription
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6 7 8 9 10
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Date Time Matrix
Number Containers ofeach
Presmatives
3mpaqt~ 3M Pmpa Lead
3M ftpm
3m DIPbvvwg Ub nowmv!
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Samples Split See Affach
Comments
12 13
Item 0
RelinquispedBy/Affiliation Time
Date
7 5@-6;
Shipped Via
Received By/Affiliation Time
Ong@ AmwnponydV SamPies
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