Document 3N922b9e1O67mjr3krkO091qx

BACK TO MAIN ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_____________________________________ Identity: Perfluorooctanoic acid, tetrabutylammonium salt; may also be referred to as PFOA tetrabutylammonium salt, tetrabutylammonium perfluorooctanoate, N2803-2 or as a major component of L-13492. (Octanoic acid, pentadecafluoro-, tetrabutylammonium salt, CAS # 95658-53-0) Remarks: The 3M production lot number was 2. The test sample is referred to by the testing laboratory as N2803-2. The T.R. Wilbury study number is 889-TH. The purity of the sample was not sufficiently characterized, although current information indicates it is a solution of 44.9% tetrabutylammonium perfluorooctanoate, 27.9% water, and 27.2% isopropanol. The following summary applies to the test sample as a mixture of the test substance in an isopropanol/water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD___________________________________________________ Method: U S. EPA-TSCA Guideline 797.1300 Test type: Acute static GLP: Yes Year Completed: 1996. Species: Daphnia magna Supplier: Obtained from cultures maintained by T.R. Wilbury Laboratories Inc, Marblehead, MA from an original culture from Aquatic Research Organisms, Hampton, NH. Analytical monitoring: DO, conductivity, pH, and temperature were monitored daily. Exposure Period: 48-hours Test organism age: < 24-hours Statistical methods: LC50and EC50values calculated, when possible, by probit analysis, moving average method or binomial probability with non linear interpolation using the computer software of C.E. Stephan. Test conditions Dilution water: Deionized water adjusted to a hardness of 160 180 mg/L as CaCO3/L BACK TO MAIN Dilution water chemistry: Hardness: Alkalinity: pH: Not noted Not noted Not noted Lighting: Cool-white fluorescent bulbs with an intensity of 110 ft-c. Photoperiod of 16-hours light, 8-hours dark with a 15 minute transition period. Stock and test solutions preparation: A 200 mg/L primary stock solution was prepared in dilution water. After mixing, the primary stock was proportionally diluted with dilution water to prepare the test concentrations. No insoluble material was noted during the test. Exposure vessels: 300 mL glass beakers containing 250 mL of test solution. The approximate depth of test solution was 9 cm. The vessels were loosely covered during the test. Number of replicates: 2 Number of daphnids per replicate: 10 Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 48 hours): 8.6 - 9.0 mg/L (control exposure) 8.7 - 9.0 mg/L (100 mg/L exposure conc.) Conductivity range (0 - 48 hours) 670 - 690 gmhos/cm (control exposure) 670 - 720 gmhos/cm (100 mg/L exposure conc.) pH range (0 - 48 hours) 8.3- 8.5 (control exposure) 8.4 - 8.6 (100 mg/L exposure conc.) Test temperature range (0 - 48 hours) 19.3 - 20.2C (control exposure) 19.3 - 20.4C (100 mg/L exposure conc.) Element basis: mortality and immobilization RESULTS___________________________________________________ Nominal concentrations: Blank control, 13, 22, 36, 60, 100 mg/L. Element value and 95% confidence interval: 24-hour EC50 = 72 (63-83) mg/L 24-hour LC50 = >100 mg/L (CI not calculable) 48-hour EC50 = 62 (36 - 100) mg/L 48-hour LC50 = 93 (74 - >100) mg/L 48-hour NOEC = 13 mg/L Element values based on nominal concentrations. BACK TO MAIN Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical component alone. Cumulative percent immobilization (inclue es mortality) N om inal Test Conc., m g/L 24-hours 48-hours C o n tro l 13 22 36 60 100 0 0 0 0 30 85 0 0 5 5 45 100 Note: at 48-hours, more organisms than noted above were affected at 22, 36, and 60 mg/L (noted as "less active than control daphnids), but were not considered immobilized. Control response: satisfactory CONCLUSIONS The test substance 48-hour LC50for Daphnia magna was determined to be 93 mg/L with a 95% confidence interval of 74 - >100 mg/L. The test substance 48-hour EC50for Daphnia magna was determined to be 62 mg/L with a 95% confidence interval of 36 - 100 mg/L. The test substance 48-hour no observed effect concentration (NOEC) was 13 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. BACK TO MAIN REFERENCES This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company, Lab Request Number N2803-2, 1996. OTHER Last changed: 4/01/01