Document 3JkyeE4gwKkoq4J5QjDDo3bxn

Microt MICROBICS MICROTOXO TOXICITY TEST TEST SUBSTANCE Identity:Perfluorooctanesulfonatmea;y alsobe referredto as PFOS or FC-95 or as partofthe mixed productFM-3820 (see Remarks). (1-Octanesulfoniaccid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassiumsaltC,AS # 2795-39-3) Remarks: The testsample isFM-3820, a mixtureofthe testsubstance in diethyleneglycolbutyletherand water (approximatel2y4-28% test substance indiethyleneglycolbutyletherand water).Calculationswere made toadjusttestvaluesforthe upper limictoncentrationofthe test substance (28%) inthe testsample and no adjustmentwas made forthe presence ofthe diethyleneglycolbutyletheror water as noted below. Calculationsassumed thatalltoxicitwyas due tothe presence ofthe Perfluorooctanesulfonatseubstance. METHOD Me*thod: MicrobicsMicrotox@ "BASIC" Procedure Test type: Staticacute GLP: No Year Completed: 1991 Species: Photobactefiumphosphoreum Analyticalmonitoring:Not available Statisticamlethods: Not available Test organism source: MicrobicsMicrotox@ Reagent Test conditions:Per Microbicsmethod RESULTS Nominal concentrations: 125, 250, 500, 1000 mg/L Test Sample Element values: 30-minute EC,o = 161 mg/L 30-minute EC5o = >1000 mg/L Perfluorooctanesulfonate concentration adjusted Element value: 30-minute EC,o = 45 mg/L 30-minute EC50 = >280 mg/L I Remarks: Values reported are for the test sample and then adjusted assuming alltoxicitiysdue tothe perfluorooctanesulfonactoemponent. No calculationwsere made to adjustforthe concentrationofthe diethyleneglycolbutyletherinthe testsample. CONCLUSIONS The testsample containing28% Perfluorooctanesulfona(tuepper limit) exhibiteda 30-minuteEC50 forPhotobacteriumphosphoreum of >1000 mg/L. Ifyou assume alltoxicitoyfthe mixtureisdue to the Perfluorooctanesulfonatteh,e adjusted30-minuteEC50 value is>280 mg/L (1000 mg/L X 0.28). Submifter: 3M Company, EnvironmentalLaboratoryP,.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking4. Values are from a summary only. Originaltestreportand raw data was notfound. Additionalldya,ta isfora mixtureand toxicitcyannot be positivelayttributetdo Perfluorooctancesulfonataes the diethyleneglycolbutylethercouldalso contributetothe toxicity. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,Lab Request number J1608, 7/24/91. OTHER Last changed: 5/3/00 08/08/1991 LABOPATM FINRL FOPM IM FG== Wi. J1608 DEPARn4aC: 0535 PRDMCT NO: 9970OL3000 RECEIM: 04/10/1991 DM: FC203 AND CCMPCmmm See T.R B2864 for D. magna test xemlts. CMCRAI= LAB: EST CMMM= N E-L TAB C=: 7 $17,600.00 E(P come (Y7/31/1991 DA3E CCMPIEMM: CY7/26/IL991 SABEME DA3E 1 2 3 4 5 6 7 8 OODE CESCFUPTMCLQ RES= BURM CAM. Ft4 8887 *AIJ2- ASSAY - IMO MCRD= - EC50 DUPCNOL HE M 3021 >1000 mm >1000 DGM *ATGAE ASSAY - IC50 bacrcmx - 91 MUL TMa"nUAZ= Et4Z7513 *AIGPE ASSAY - 1= MCFOTCOC - EC50 87 MWL 6.1 W" EUAMER EN-4115 *FISH Aam TEST - 96 HOCIT *ALGAE ASSAY - IC50 MCFC= - EC50 FIUIER EM 3820 77 MIL 14 ICIM >1000 Nr./L *AIAM@E ASSAY - MM MCRDXZ - EC50 NA-O=YLS" M4 55040 260 MM >1000 MG/L *FISH ACUTE TEST - 96 EaR *ALCAE ASSAY - IC50 MCFC= - EC50 FC-203 480 pM 170 y4m So MG/L *AIGAE ASSAY - IC50 350 bWL F'C203 W/o M V* 160390 *FISH ACUTE TEST - 96 HOOFT >1000 wIL ommwr IAB Dm mx OR r@.I.) (c.r. (C.I. (95% CI(SO-100)) (95%a(0.6-1.4)) (95% a (73-99)1 (95%= (5.8-6-4)) (95%a (60-100)) (95% C:1(13-16)) (C.I. (95%a (220-290)) (C-I- (95%M (40D-M)) (95%a (150-180)) (95%a (68-93)) (95%a (320-390)) (C-1- 08/08/1991-- ENVn%104DnAL LM3ONk=m rnal Rm?cRr LAB 10== SAKILE NU. J1608 030C 03RTINUM EESCRUIT= FCSU= FC2()311/070 VW 160390 *AT,ME ASSPLY- MW MCFD= - MC50 360 W.IL 320 W.IL SaM4ETIM BY: DA3ED: 2 ML CiFt(C.I.) (95%a (310-aO)) (95%a (300--350)) EnvironmentLaalboratory RequestForm FOMMM-PI-PM FWW NoWme a& 3M Environmental Laboratory Building2,3E-M P.O. Box 3=1 SL Paul,MU 55133-=l Attention: Ana"cal Coordinator (612)7784M am-" SriA 1:@@ pool4a 000N.Sftd 0 Routine po ofD"A 0,3 0- t- Compomn+S DOL Ckww 0 W12 C! OM mf ly PM" ON* ft. Cqii EnvkorananW Lmb 7111 f-< A Conbuct Lob II 7/ 1rgi TOW C.0 0 RCRA 0 TSCA E3 CTVA 0 CAA 0 SDWA 0 MSOS 0 "a conmwts -For rnel -ter.+ f-e&ul+c- SwnploNo. SampleCodo-----MCNi@w@ SampleDele SampleDescrigion -TMt PWWASOW C, ip-im Coft AlpAe- b,-pc.%DiAr- Tolytr&kMt Fbctnw-c 2997 VI 30;t 1 ;7511?, ps AM-Or-*IQACc.e--.go3 M4o3w/,-, FAd3g.2 0 I?M -59*aff Viv it.-3,4q @cro+ox COMY-P MM AGOW Environmental Lab. Data Report Form Parameter ro+ox C,0,0&A"0 "M inu 7 7 LR t )bps - Result > /004c> q5 Unit rnall. Date comfftonts tnt" FicA2s-Lre qg4@6CI > ]Do o 1 -Tlkos - -5- /000 i-cs-o 'go Cit (3t,-o350) 7//g lq) 7 //IllqI 7 EWRONk4EWAL UUX)RATMY LICFK)BICS MKAOTCXO SYSTEIA 11608 7124/91 TO)OCITY iESi RESLJLTS PERCENT UGHT LOSS TWCOW- I" 1.25 2.5 3.125 5.0 6.25 10 12.5 2531.26 50 62.5 ioo 125 250 Soo 1000 BUTYLCAROrrOL .DUPCNOLUE ..TOLYTFAAZOLE FOAMM FRAIER RM OW FDA 3021 AM 27513 FU 4115 FU 3= I-AM LMW IS--'-39,@M S:MIA llagl 26--,LIM 3&dlb 20.-M &a& I!L.-A, ag@M 20 41 S4 39- 67 79 32 30 32 74 89 92 51 49 52 87 92 es sa 97 70 82 90 82 P64OCrtLMX.FA7E FU 32M ift'BXYTRLAZOLE SAM lhmb 32:dm szsft 12 10 14 17 19 19 31 20 31 40 46 40 24 as IL7 17 n 4.4 7.1 Lt 37 SS 10 14 20 13 17 23 54 67 It 1& 21 19 24 31 -23 -27 30 23 30 42 47 &B 76 17 30 " 19 _29 30 42 49 64 64 76 ECW -10" -100 -ION 3.0 1.0 1.1 &2 LO Ll IOM .110M .100 .,ON -a-% C-1- .100 M lag go -Iwo a" an