Document 3JRRwnjzxZj4JMJjeOXow3Rrx

AR226 - 3257 439 TRADE SECRET AR2RG 3257 Dupont 11408 Study Title H-25434: Static, Acute, 96-Hour Screening Test to Pimephales promelas Laboratory Project ID: DuPont-11408 AurHoR: Laurie D. Bouchelle Alan Samel, M.S. ~~ STUDY COMPLETED ON: October 2, 2002 PERFORMING LABORATORY: EHa.sLkdelulPLoanbtordaetNoerymofuorrsHeaanldthCaonmdpEannvyironmental Sciences Elkion Road, P.0. Box 50 Newark, Delaware 19714-0050 work Request Novex: (EIR Stupy Cove Nove:(EIR Page 1017 `Company Saniized. Does not -- contain TSCA CBI S12e5n4i3n4 TSea1,0 Aicnic,h9es-Hromelas _ Durenc1408 CERTIFICATION `We, the undersigned, declare that this report provides an accurate evaluationofdata obtained from this study. owetaayDbistyr: _ A uinig Lr inT n (o. aie Sees 2belobDeieadooR --- ~~ = GompanySaiizad,Doesno containTSOA GB 1S.c2r5e4e3n4i:ng TSeastit,oAPincepuh9at6leHesopur,ormelas STUDY INFORMATION Substance Tested: JENIN Svnonvms/Codes: + H-25434 Haskell Number: 25434 DuPont-11408 or = Known Impurities: (NY Physical Characteristics: Off-white, milky semi-opague aqueous dispersion Stability: The test substance appeared to be stable under the conditionsofthe study; no evidence of instability was --_ observed. Sponsor: E.L du Pont de Nemours and Company Wilmington, Delaware 19898 USA. StudyInitiatedCompleted: September 23, 2002/ (see report cover page) In-Life Initiated/Completed: September 23, 2002 / September 27, 2002 P-- `CompanySanitized. Does notcontain TSCA CBI 1.25434: Static, Acute, 96Hour ScteeningTestto Pinphalespromeles --~ Duowllh SUMMARY "The acute toxicityofH-25434 to the fathead minnow, Pimephales promelas was determined in an unaerated, 96-hour, static test "mTehae nsttuedmypewraastucroenodfuc2t2e.d9wiCt(hr4ancgoenocfen2t2r.a9t-i2o3n.s0oCf)H.-2O5n4e34tesatncdhaamdibleurtiwoan swautseerdcpoenrtrtoelstata ccoonntcreonltraantdiotnhewi1t.h0 a1n0dte1st0omrgg/aLnitsesmtscionnceeancthrcahtiaomnbserw.erBeacsleeadrownitvhisnuoalcooblsoerravattiesotnsst,artt.he Twahteer t1e0s0t cmogn/ceLnttersattcioonncweantsractliooundywawsitchleaanrowilyisauntrofiahcley fsiulrmfaatcetefsitlsmtaart.tesAtllstwaatt,aenrdqutahleity10p0a0rammget/eLrs were within acceptable limits during the exposure. E1x00p,osaunrdeo1f00r0aimngb/oLwrtersouultteod itnhe0,wa0,te0r, c2o0n,taronlda1n0d0n%ommiorntaalliHt-y,25re4s3p4ecctoinvceelnyt,raattitohenseonfd o1,f10, h9i6ghhoeusrts.nomNionamlorctoanlcietnytorratsiuobnlectahuaslinefgfnecotsmowretraelisteyean itnesttheenwdatwearsco1n0tmrogl/Lt.estTohregalnoiwsemsst.nTomhienal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-25434 concentrations were used for calculation ofLCso values. -- "The results are summarized as follows: Nominal concentrationsof H-25434, mg/L | dilution water control, 1, 10. 100, and 1000 96-hour LCso (95% fiducial limits) for | 181 (62 to 448) F-25434, based on nominal concentrations, mg/L" H-25434 exhibited low concern for aquatic hazard" in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas. FPreeltsihewr,atWe.rHa.nnddMaWreibneer,OrCg.a1niEsdmss..(1E9P8A5J)G.OMOe/4t-h8o5d-0s1f3o.r MUenaistuerdiSntgatthees AEcnuvtieroTnomxeinctiatlyoPfroEtfefcuteinoinsA0gency, Environmental Monitoring and Support Laboratory, Cinciiti, Ohio. ~~ * `(SmWae.tcGh:hoedLs,anaJdn,idsC,elve1am5le.unaHttuisog,nhReo.sf,d,Maoatrn.dsoMcE.knAv,.iRrL.o,enwaminesdn,RtEaadlbsT)es,,ipcWpo.i2o2(g-1y39a.9n3d)ARmAiessrsiekscAsasinneSgscoscmieeotnyf,oAlrSTeThsoMatSziTangrgPdnu1ndi1d75eM.arctTerSiCallAs:, Philadelphia. J re ---- : `Company Sanitized. Does notcontainTSCA Cal S1c.r2e5e4n3i4n:g TSteattic1, APciumteep,h9a6l-eHsopurromelas --_~ MATERIALS AND METHODS DuPont 11408 A. Test Solution Preparation TneosmtinsaulbsctoanncceentsroaltuitioononfstweesrtespurbesptaarnecdebsyolduitrieocntsawdedirteionnootfadHj-u2st5e4d34fortopudriiltuyt.ionTewsattesro.lutTihoens cwoenrteaipnreedpa1r5edLobyfaHdadsiknegllthLeabaoprparotporriyatweelalmwoautnetroafntdessttisruibnsgtafnorceaptporcoaxcihmatetsetlych3a0mmbienrutwehsi.chThe `wTahteer10co0nmtrgo/lLatnedsttchoenc1.e0niarnadti1o0nmwga/sLclteeastr cwointchenatnroaitliyonssurwfearcee fclielamrawtittehstnsotarcto,laorndatttheest start 1000 mg/L test concentration was cloudy with an oily surface film at test tart. B. Dilution Water aDinldutsieoanlewdaitnetroobreidgrioncakt.edTfhreomhatrhdenHeasssokefltlhLeabwoerlaltwoartyewrelislawdhjuiscthedist3o7a8p-pfreeotxidmeaetpelaynd is cased a1e0r0a-t1ed4,0pmags/seLdatshCroauCgOh,abgyretehne sflaonwd-fpirolptoeorrtiroenmeodvaeddiirtoni,onaonfdCfailCtle.red Tthhreowueglhl5w0-a,te1r0-i,s atnhedn --- d0i.s4t5r-ibpumtefdilttherrsotuoghreamgoevdepoplayrvtiicnuyllatcehsl.orTihdee pwiaptienrg.isThheeatdeidluotricohniwlaltederasisaapnpraolpyrzieadtetwaincde yearly for major anions and cations, metas, total organochlorine and organophosphate pesticides, and sppoelcyicfhilcoartiionnast.ed biphenyls. The dilution water meets OECD) and ASTM criteria and C. Test Organism Culture Faa4t0h-eLa,drmecitnannoguwl,arP,igmleapshsahloelsdpirnogmtealnaks,(Sw0e-rcemrleeanrgetdh,at2H5a-sckmelwlidLtahb,or3a1to-rcym. deTphteh)fifsohrw1e0r1edhaeylsd iinn rcoanntgienduforusolmy2-1fl.o9wtion2g2w.e1llCwa(tmere.anD2u2r.i0nCg).theT7h-edafyisphewreiroed pfreedcferdeisnhgiyt-hheattceshte,dw, laivre btreimnpeersahtruirmeps (San Francisco Bay Brand, Inc.)/frozen brine shrimp (San Francisco Bay Brand, Inc.VAquaMax `SotnacretedraiFliyngoenrlwienegk3e0n0dsSDa0n3d h(oPlMidIaysN.utrSiitcikonnesIsn,teirnnjautriyo,naaln,dLvLisCi)bltewaibcneodramiallyitoinesweweekrdeanyost and psreeesnendturiinnfgitshhein48thheohuorlsdpirnigorttaonuksneorfoirn ttehsetifnigs.h uFsiesdh fwoerrteesitdienngt.ifLieedssbythlaanbe0l.s5o%nmtohretahloiltdyinwgas tanks and test chambers. D. Test Methods ~~ Fnooumrinnaolmitensatlcotnesctenctornacteinotnrsatwieornse aI,nd10a,d1i0l0u,tiaonndwa1t0e0r0cmongt/rLolHw-e2r5e43u4s.ed in this study. The ------ iS ----e--------ER----------eeeee Company Sanitized. Does not contain TSCA CBI 1Sc2r5e4e3ni4n;g TSetsatti, APciumteep,h9al6e-sHopurromelas DuPont11408 ~ Taepsptrocxhiamnaitbeelrys w1e5rLeosfttaeisntlesssolsutteieolna(q2ua6r-iLam[a30xi(lmeungmthv)olu3m0e;(w1i7d.t5h-)cxm3te0st(hseoilguhtti)oncmd]eptwhh)i.chOnheeld test chamber was used per test solution (total of 5 test chambers; 10 fish in cach test chamber). Each chawmasbcoveerred witha glass plate to prevent the fish from escaping. `cAodmdpilteitoendo.f fMiosrhtatloitthyeatnedstbseohlauvtiioornsalwoabsseirnvitaitaitoendsafwteerremimxaidnegeovfetrhye2t4eshtosuorlsutainodnsatwatesst end. wDeeraedtfhieshawbseernecreoemfoovpeedrcfurloarm mthoevteemstencthaamnbderlsacdkuorfirnegadcatiiloynotbosegrevnattlieopnrs.oddCirnigte.riAatfotresdteath conclusion, all fish were humanely sacrificed using MS222 (3-aminobenzoic acid ethyl ester). Athere9c6i-rhcouluartitnesgtwaattaeprpbraotxhimwaatseluyse2d2.t9omCaiwnittahian mraenagneotfem2p2e.r9attuor2e3.in0tCh.e tAestphcohtaompbeerrisodduorfing i1n6chlouudresd l3i0ghmti(nauptpersoofxitmraatneslityi1o8na2ltoli4g4ht2(2L5uxt)o a2n1d1 Luhxo)urpsredcaerdkinnegssanwdasfoelmlpolwoiyngedthweh1i6c-hhour light interval. mDiesassoulrveemdeonxtysgweenrecotnackeentnrbaetfioorne, fpiHs,h awnedreteamdpdeerdatautrteeswtetraertm,eeavseurrye2d4inhoaullrstetshtecrehaafmtbeerr,sa.ndTahtese: test end. Test solutions were not aerated during the test and were disposedofin an appropriate. manner at test end. REFERENCES 1. Organisation for Economic Co-Operation and Development (OECD). Guideline for Testing Chemicals: 203, 17 July 1992. 2. American Society for Testing and Materials (ASTM). (1988). Standard Guide for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians. E 729-88a. Annual Book of ASTM Standards, Vol. 11.04. --~ --- Company Sanitized.DoesnotconTtSaCAiCnBI 125434: Static, Acute,96Hour ScreeningTestoPinephalespromelas _ Dionwlld08 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-25434 Dissolved Concentration mg Oxygen (mg p Test Completion (96 hours) Dissolved (Omxgygen p `Water Control 85 1.0 84 10 84 100 8.4 1000 84 77 6.4 15 7.6 72 | 77 75 71 76 74 62 75 73 7.5" 73 a Measurement performedat 24 hours duteo total moray. --_ TABLE 2 MORTALITY OF Pimephales promelas IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-25434 `Nominal H-25434 Concenmtgration NumberofDead Test Organisms / NumberofTest Organisms at Study Start [24Hows | 48Hows | 72Hous | OGHows | `Water Control 1.0 10 100 1000 0/10 0/10 010 010 10110 010 010 0/10 10 1010 010 010 010 1/10 10/10 010 0/10 0/10 210 10/10 -- eon--------------A --------i-- eee-- Seeet rct CompanySanitized. DoesnotconTtSaCAiCnBI