Document 3JREr0mG98gxbM46615meqqRO

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (EASTERN OYSTER) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-203 (F5919-1).Currentinformatioinndicatesitisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol, 2.66 % Sultonefoamer, 3% sodium octylsulfate0,.1% sodium laurylsulfate, and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Standard PracticeforConducting StaticBasic Acute Toxicity Testswith Larvae of Four Species of BivalveMolluscs(ASTM 1980). Type: Acute static GLP: No Year completed: 1980 Species: Crassostreavirginica Supplier: Inducedspawning inlaboratory-maintainmeadture adultoysters Analyticalmonitoring: Temperature,pH, salinitayn,d DO. Exposure period:48-hours Test organism age: Embryos,within1 hour afterfertilization. Statisticamlethod: Test concentrationcsonvertedtologarithmand correspondingpercentage reductionof normal larvaewas convertedto a probit.EC50 values then calculatedusing linearregression. Test conditions: Dilutionwater: Filterednaturalseawater pumped from Big Lagoon, a Gulfof Mexico estuaryadjacenttothe laboratoryP,ensacola, FL. Dilutionwater chemistry: Salinity:20 ppt Lighting: Not given. Stock and testsolutionpreparation: A primarystocksolutionwas prepared by adding a weighed amount oftestsubstance tofilteresdeawater. Exposure concentrationswere then prepared by additionofthe appropriate volume of stocksolution. Exposure vessels: I L glassbeakers containing900 mL oftestsolution. Number of replicates:3 Number of organisms per replicate:Approx. 25,600 embryos Number of concentrations:fiveplusa blankcontrol Elementbasis: Numberofnormalldyevelopeldarvaceo,untewdith Sedgwick-Raftercell Water chemistry during the study: Temperature range (0-48hours): 21 1 *C (temperaturecontrolled environmentalchamber). Salinityrange (0-48hours): 20 ppt pH range (0-48hours): 7.7-7.8 Dissolved oxygen range (0-48hours): >74% saturation RESULTS Nominal concentrations:Bk control6,,10,32,56,and 100 mg/L. Element values: 48-hourEC50 = 47 (10-234) mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conductedon themixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsubstance48-hourEC50 was determinedto be 47 mg/L witha 95% ConfidenceIntervaolf 10 to234 mg/L. Submitter: 3M Company, EnvironmentalLaboratoryP,.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudymeets the criterifaorquality testing.However, thesample puritywas notpropedycharacterizeadnd the study lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportion inthe solution. REFERENCES Testwas conductedby EG&G, BionomicsMarineResearch Laboratoryo,f Pensacola,FL atthe requestofthe3M Company, Lab Request number 4971, Sample 5729, 1980. OTHER Last changed: 6/27/00 Acute toxicity of Sample #5729 to embryos-larvae of eastern oysters (Crassostrea virginica) rc- z a3 rc-=z 03 FS 919- Cc-I-0:5- Toxicity Test Report Submitted to 3M Company St. Paul, MN 55133 Project Number L55 Report Number BP-80-7-117 EG&G Bionomics Marine Research Laboratory 10307 Gulf Beach Highway Pensacola, Florida 32507 July 1980 .A marine toxicity test was conducted at EG&G Bionomics Marine Research Laboratory (BMRL), Pensacola, Florida, to determine the effect of Sample #5729 on embryos-larvae.of eastern oysters (Crassostrea virginica). The criterion for effect was reduction of the number of normal larvae (those which developed to the fully-shelled, straight-hinged veliger stage within 48 hours) in test concentrations as compared to the number of normal control larvae. Results of the-test are expressed as a 48-hour EC50 (the concentration of test material estimated to be effective in preventing normal development of 50% of the exposed embryos-larvae). Data from the test are maintained at BMRL. MATERIALS AND METHODS Test material The sample, received at BMRL on 12 June 1980, was contained in a 0.9-liter (t) clear glass bottle labeled "3M Company, Sample #5729." The sample was an orange liquid. Test conce"'ntrations are reported here as milligrams (mg) of whole material per Y.of seawater or as parts per million (ppm). Test animals Oyster embryos were obtained by induced spawning of sexually mature adult oysters which were maintained in flowing, unfiltered seawater at BMRL until testing began. Test water. Water used for spawning and testing was natural seawater which was pumped from Big Lagoon, a Gulf of Mexico estuary adjacent to BMRL. The pump intake was about 80 meters (m) offshore at a depth 2 of approximately 3 m. Seawater was pumped by a #316 stainless steel pump through hard polyvinylchloride (PVC) pipes, through sandfilled fiberglass filters, and through 10-micrometers (um) pore size polypropylene core filters into an elevated fiberglass reservoir.' Water was aerated in the reservoir and flowed by gravity through PVC pipes into the laboratory. There it was.pumped through a 5-um pore size polypropylene core filter and distributed into the spawning or.test chambers. The chemical composition of BMRL seawater is characterized in Appendix A. Test conditions Methods for...the48-hour oyster embryo-larvae test were based on Standard Practice for Conducting Static Basic Acute Toxicity Tests with Larvae of Four Species of Bivalve Molluscs (ASTM, 1980). In'dividual, sexually mature female oysters.were induced to spawn by placing them in glass chambers containing 1 t of filtered seawater at 25 degrees Celsius (OC) and increasing the water temperature to approximately 300C in the presence of viable sperm excised from the gonad of a sexually mature male oyster. Fertilization occurred upon release of.the eggs into the spawning chambers and was confirmed microscopically. Fertilization success was estimated to be >90%. Density of the embryos was determined by a Sedgwick-Rafter count of a 1:10 dilution (1 mi embryo suspension:9 ml of seawater) from the spawning chamber. All concentrations and the control were triplicated. Test containers were 1-k glass beakers, each of which contained 900 mi of filtered, natural seawater. A primary stock was prepared by adding a weighed amount of test material to a known volume of 3 filtered seawater and the appropriate volumes were added to each test container to obtain the desired test concentrations. Each test container was inoculated with an estimated 25,600 embryos within 1 hour after fertilization and then maintained at 2110C in a temperature-controlled environmental chamber. After 48 hours of exposure, the larvae from each container were collected in a 37-um mesh size sieve, rinsed into a plastic bottle*with 24 mt of filtered seawater, and preserved with 1 mi of neutralized formalin. The number of normally developed 48-hour larvae was determined by a Sedgwick-Rafter count from each triplicate test and control container. -Percentage reduction of normal larvae was determined as follows: Percentage reduction Number of normal 48-hour control larvae minus the number of normal 48-hour larvae in each test concentration - x 100 er of normal 48-hour control larvae The test was conducted 1-3 July 1980. Statistical analyses Each test concentration was converted to a logarithm and the corresponding percentag e reduction of normal larvae was converted to a probit (Finney, 1971). The 48-hour EC50 and 95% confidence limits were then calculated by linear regression. RESULTS AND DISCUSSION The calculated 48-hour EC50 for embryos-larvae of eastern oysters exposed to Sample #5729 in static, unaerated seawater wa- No significant reduction of embryos-larvae which developed normally to the straight hinged veliger stage after 48 hours occurred in 4 6 or 10 ppm,,but a significant reduction of normal larvae did occur in concentrations >32 ppm (Table 1). ]@leasuredconcentrations of dissolved oxygen remained >74% saturation and the pH was from 7.7-7.8 after 48 hours of exposure (Appendix B). 5 REFERENCES ASTM.Standard E. 724-80, Practice for Conducting Static Acute Toxicity Tests with Larvae of Four Species of Bivalve Molluscs. American Society for Testing and materials, 1916 Race Street, Philadelphia, PA 19103. Finney, D.J. 1971., Probit Analysis-'. Cambridge University Press, London. 333 pp. 6 TABLE 1. Toxicity of Sample #5729 to embryos-larva e of eastern oysters (Crassostrea virginica) exposed for 48 hours in staticp unaerated seawater. The criterion for effect was the reduction of the number of normal larvae in test concentrations as compared to the number of normal control larvae. The initial inoculum was 25,600 embryos. Salinity was 20 O/oo and temperature was 210C. Nominal .concentration -(Mg/t;ppm) Control 6 10 32 56 100 Number of normal larvae Mean SDCL 21,612 2,478 22,657 2,151 22,373 2,010 16,530 2,707. 11'1258 1,054 380 142 a Standard deviation. Reduction of normal 48-hour larvae ----- 24 48 98 PREPARED BY*. AUDITED BY: REVIEI-IED BY: APPROVED BY: 7 Terry A. Hollister Study 06irect!(* Tom Heitmuller 1-Uual'ity Assurance Un-tt Peter J. Shuba, Ph.D'Technical C6ordinator Rod Parrish APPENDIX A Results of Chemical Analyses for Routine Characterization of Selected Ch Constituents in Bionomics Marine Research Laboratory Seawater Chemical Constituent Arsenic Cadmium Chromium Copper Mercury Nickel Zinc Lead Total Phosphate Ammonia Nitrogen Nitrate Nitrogen Nitrite Nitrogen Total Petroleum Sulfides Pesticides Polychlorinated as P as N as N as N Hydrocarbons Biphenyls -Concentration 1979 Rangea <0.001-0.006 <0.01-0.002 <0.01-0.008 <0.01-0.02 <0.0005-0.0007 <0.01-0.02 <0.02-0.05 <0.02 <0.02 0.14-0.42 <0.01 <0.01 <5.0 <1.0 None detectedb None detectedd (mg/Y.;ppm) I AP3 <( <( <( < < < < None None Water samples were collected from Bionomics Marine Research Laboratory sez system after the.mixin(j station in the wet lab. arange of concentrations are based on two sampling periods. bpesticides: BHC, lindane, heptaohlor, heptachlor epoxide,,aldrin, perthane, DDE, TDE (DDD0)5,,ugD/DlT, methoxychlor, endosulfan,, strobane, and chlordane all <0.0 ;ppb.., cpolychlorinated Biphenyls: Aroclore 1016, 1232, 1248, 1260, 1221t all <0.05 vg/k;ppb. dield2 toxapt 1242,, APPENDIX B QUALITY ASSURANCE FORM OyS-rER-EmBRyo TOXICITY TEST NO- SP-QAF-DE-001 Effective: April 1980 Revision: 01 Page- 33ECT: 48-HOUR STATIC DATA SHEET CLIENT, PROJECT NO: TEST 10AIrERIAL: TIME INOCULUM ADDED: TIME TEST-MATERIAL ADDED: )/00 0 HOUR MEASUREMENTS IN CONTROL (REPLICATE A) woe-. SALINITY: 7Et4PEP,'ATURE: (See Table Below for Data by) TEST HOUR DATE/TIME DATA BY :@SURE14EN"c 0-iCENTRATION 0 14OUR DISSOLVED OXYGEN (mg/1) REP A REP B REP C 0 L) PH 48 HOUR DISSOLVED OXYGEN (mg/1) REP 8 REP C PH REP A 0 o 0 to -73 kr\ IOL '7, -TJAL ItiOCULLIM: liumberof normal eribr@-foosund in 1 nl x 'D,'mbe, of fiellis @edgwick Rafter x FacLor 'Al@CULA.T!DN: /l,q -@/0- Dilution Factor Total nunber of normally developed embryos per ml a6() C711-tc-.@