Document 3J02kzwxRjy3jDQzZKk6emByx

ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may also be referredto as PFOS, FC-95, oras a component of FC-201 F. (I-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium saltC,AS # 2795-39-3) Remarks: The 3M productionlotnumber was 1. The testsample is FC-201F(F-11129). CurrentinformatioinndicatetshatFC-201F isa mixtureof 3.0% PFOS, 36.0% diethyleneglycolbutylether,36.6% water, 8.25% acrylicfoamer,7.0% sodium octylsulfate6,.0% sulfonatedalkyl ethersodium salts,3.0% triethanolaminea,nd 0.15% tolytlriazole. The followingsummary applies to a mixture with incompletely characterized concentration of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline202 Type: Acute static GLP: No Year completed: 1992 Species: Daphnia magna Supplier:In-houseculturesm,aintainedatAScl-DETD, Duluth,MN. Analyticalmonitoring:Temperature,pH, conductivitaynd DO Exposure period:48-hours Test organism age: <24 hoursoldneonates Statisticamlethod: EC50 calculatebdy Trimmed Spearman-Karber method. NOEC calculatedusing Dunneff'sprocedure. Test conditions: Dilutionwater: Shallowwellwater from Two Harbors,MN. Dilutionwater chemistry: Hardness: 170 mg/L (as CaC03) Alkalinity: 147 mg/L (as CaC03) pH: 8.61 LightingC:ool-whitfeluorescenltightpsrovidinagdailyphotoperioodf16 hours lightand 8 hours dark. Stock and testsolutionpreparation: A 1000 mg/L primarystock solutionwas prepared by dissolving1000 mg FC-201 F inone L ofdilution water. Remaining concentrationsprepared by sehal dilutionosfthe stock solution. Exposurevessels:250-mLborosilicgaltaessbeakerscontaini2n0g0 mL testsolution. Number of replicates:four Number of daphnids per replicate:5 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: Conductivity range: (0-48hours) 330 - 349 pmhos/cm (controls) 371 - 376 pmhos/cm (1,000mg/L exposure) pH range (0-48hours): 8.51- 8.61 (controls) 8.47- 8.48 (1,000mg/L exposure) Temperature range (0-48hours): 20.4- 20.6 *C (allexposures) Dissolved oxygen range (0-48hours): 7.5- 8.0 mg/L (controls) 7.1- 8.0 mg/L (1,000mg/L exposure) RESULTS Nominal concentrations:Blank control6,2.5,125,250, 500,and 1000 mg/L Element values: 24-hour ECFO = >1,000 mg/L 48-hour EC50 = 308 (272 -348) mg/L 48-hour NOEC = 125 mg/L Element values based on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not thefluorochemicalproportionalone. CONCLUSIONS The FC-201 F 48-hour EC50 was determined tobe 308 mg/L witha 95% confidenceIntervaolf 272 to 348 mg/L. The 48-hour No Observed Effect Concentration(NOEC) was 125 mg/L Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.This study meets the criterifaorquality testing.However, the sample puritywas not propedy characterizedand thestudylacksanalyticaclonfirmatioonfthe amount offluorochemical proportioninsolution. REFERENCES Thisstudywas conductedby AScl CorporationD,uluthEnvironmental TestingDivisionD,uluth,MN atthe requestofthe3M Company, Lab Request number K1792, 1992. OTHER Lastchanged: 6/26/00 AS.CICOMOMdon/AScl.Dubish famnm" TestinDgivW= ASCIReporMt# 015-DioEA.R3M ASelStudMy# 5030-01541 ACUTE TOXICITY STUDY TITLE OF FC-201F TO DAPHNIA MAGNA DATA STANDARD OECD GUIDELINE 202 STUDY COMPLETED August 21, 1992 TESTING FACILITY' ASCI corporation AScI-Duluth Environmental Testing 112 East Second Street Duluth, MN 55805 Division Tel. No. (218) 722-4040 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5030-015-01 3M Company Lab Request# K1792 Sponw. 3M Company SponsorStudyM# K1792 ASel corporation/ASel-T'A-bm'6 Eovkmn=W Tegim DWisim AScl Repon M# 015-DMA.IUM ASCISb* M# 503"15.01 1.0GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Directo Definitive Test Dates Data Validation and Report Review Retention of Raw Data and Final Report Location of Raw Data and Final Report Acute Toxicity of FC-201F to Daphnia magna OECD Guideline 202. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106.. Susan A Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452.-- K1792. ASCI corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Dave Nessa Dinesh Vaishnav Donald Mount August Dinesh 17-19, 1992. Vaishnav Report Signature: Two years from the date study is completed. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, IMN 55805; Tel. No. (218) 722-4040. Sponsor3.M CoaW=y sponSsU"*M#KIM 2 ASelcm"ratkWASel.DubA EnvkcamcatdTali% Div@ A&I ReponM# 01-%-DMA.R3M ASclft* M# 503"15-01 2.0OBJECTIVE(S) To determine the 48-h EC50 and 48-h NOEC (no observed effect concentration) of the test substance, FC-201F, for Daphnia magrna. This study was conducted according to the ASCI study plan Iretained by the Sponsor and OECD Guideline 202 (OECD 1984). 3.0 TEST METHODS Test Substance Test organisms Dilution Water PXoperties: FC-201F, orange/red liquid, relative density 1.1 (water = 1), stable, and miscible with water. Test concentrations: 0.0 (dilutionwater control), 62.5, 125, 250, 500, and 1,000 mg/L, prepared by a serial dilution of 1,000 mg/L stock solution. Throughout the test, all test solutions appeared clear with a persistent foam at the surface. Source: Healthy, less than 24-h old Daphnia magna neonates obtained from ASCI stock culture. Use in test: Gravid.adults isolated in dilution water 24 h before the test initiation. Used 5 organisms/replicate and 4 replicates/test concentration. Handled with a smooth bore pipet with an inside diameter of 6 mm. Did not feed during the test. Source: Shallow well water from the Two Harbors, Minnesota area. The most recent chemical analysis is in Raw Data Package. Chax-acteristics: At test time, a hardness of 170 mg/L (as CaC03) and a pH of 8.61. Aerated for 24 h prior to using in the test. Sponso3rM Compmy SpomwSaWyM# K1792 3 AScicaparatkWASel.Duhah EavroomeaWTestmaDmsm ASCERepM ID#015-DMA.R3M ASclStudIyD#5030-01541 Exposure Chambers Incubation Observations Endpoint Calculations Type: 250-ml borosilicate glass beakers. Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observa- tions were made. Duration: 48 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 20.4-20.60C. Biological: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure chambers were gently agitated). Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry: (1) At test initiationtotal hardness and alkalinity, (2) dailytemperature, and (3) at test initiation and termination- DO, pH, and specific conductivity. All determinations were made according to APHA methods (1989). ECSO: Trimmed Spearman-Karber (Hamilton et. al. 1977). method NOEC: Dunnett's procedure (TOXSTAT statistical software, version 3.1, University of Wyoming 1989). Sponso3rM Company Spm" StudyM# K1792 4 ASCIampmaica/ASel-DubAh EovkonmmW TesfinEg*visim A&I Repon M# 01-'-DMA.MM A&I St* M# 5030-015-01 3.0XD%-'LcLSULTS Test Substance Toxicity (Pertinent data are in Table 1) 24-h EC50: >1000 mg/L. 48-h EC50: 308 mg/L. 48-h NOEC: 125 mg/L. Ranges of Water Chemistry Parameters Total hardness (as CaCO3 mgll): 170-186. Alkalinity 147-152._ (as CaCO3 mgll) Temperature (OC): 20.4-20-60C -Do (mglL): 7.1-8-2. pH: 8.47-8.64. specific conductivity (umhoslcm): 330-372. 4.0 TEST QA/QC QA/QC criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance toxicity tes (thistest is optional). Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 80% of the air saturation value at 20.40C. The test duration was 48 h. Final report for the most recent test with sodium chloride is in Raw Data IPackage. Spmaw. 3M Compmy SponsorSu* M# K1792 5 AseicorpmaiagAsel-D" Euvwoom=W Taft% D"m ASCIRepm M# OIS-DMA.R3M ASCIft* M# 3030-0t.-@41 5.0REFEP@ENCES American Public Health Association (APRA). 1989. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Hamilton M.A., Spearman-Karber concentration 11:714-719. R.C. Russo and R.V. Thurston. method for estimating median in toxicity bioassays. Environ. 1977. Trimmed lethal Sci. Technol. organization for Economic Cooperation and Development 1981/1984. OECD Guidelines for Testing of Chemicals. Publication Information Center, Washington, D.C. (OECD). OECD University of Wyoming. 1989. Toxstat Version 3.1. Fish Physiology and Toxicology Laboratory, Department of Zoology and Phys.iology. University of Wyoming, Laramie, WY. Sponso3rM. Company SpommSWy M# X1792 6 ASetcorporadon/AScl-Dubah Envkmnenw Tedwg Division ASclReporMt# 015-DMA.R3M ASEISO* M# 5M"15-01 Table 1. Acute toxicity of FC-201F to D. magn& Test substance nominal concn (mg/L) 0.0 62.5 125 250 500 1000 Cumulative organisms' number and % of dead and imobilized 24 h 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 4 (20) 48 h 0 (0) 0 (0) 0 (0) 4 (20) 20 (100) 20 (100) apercentages are in parenthesis. ECSO and NOEC 24-h ECSO 48-h EC50 48-h NOEC Effect and no-effect concentrations with corresponding 95% confidence intervals Data (mg/L)" >1000 mg/L 308 mg/L 125 mg/L 95% Confidence Not reliable 271.89-348.42 Not applicable interval BECSO values were calculated based on the test substance nominal concentration. Spoww.3M Company SponsorSa* ID# K1792 7