Document 37QEGqnmqnm19vZvLRvdxoaxJ

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST J TESTE SUBSS TANCT E ~S~U ~BS 0 T0 AN0 GE Identity: aPlesroflbuoeroroecfteyrrlesdultfoonaasteF,ludoirdoealckyylldaimmemtohnyilaummmodenriiuvamtisvael.t; may [1- D1,e1c2a.m2i:3n,i3u,m4,,4,N5-,d5e,c6y,l6-,N7,,N7-,d8i,m8e8t-hhyelp-t,adsaelctawfiltuhoro-1- octanesulfonic acid (1:1), CAS#251099-16-8] RL-e1m4a3r9k4s:referTrehdet3o Mbyptrhoedutcetsitolnablootrantuomrbyears was Lot P3025. 1. The test sample The sample was is ilnabwealteedrF(-a1p1p6r1o5x,imLaottel1.y 3T0h-e40te%sttessatmspulbestisanacem,ix6t0ur-e7o0f%twhaeteters,tasnudbs0t-a5n%ce oTfhreetseisdtuaslapmeprlfeluaoprpoechaermsictaolbse).aA2ll-pvhaalsueesdirseppeorrstieodnr(ecllaetaertloiqtuhiids wmiitxhture. opaque solid) were made to which adjust rapidly for the sacetpuaarlatceosncaefntterraatgiiotantioofn.theNtoesctalscuublsattiaonncs.e in the test sample. METHOD Method: OECD 209 Testtype: Static acute GLP: No Year completed: 1997 Analytical monitoring: dissolved oxygen. Nominal concentrations. pH, temperature and Exposure period: 30-minutes and 3-hours TSteasttisotricgaalnmiestmhsoodusr:cen:otAacptpilviactaebdlseludge mixed liquor collected from IMnetorcoulWuamstmeiwxaetderlTiqrueoartmseunstpPelnadnte,dSstolPiaudls,: MN 4.38 g/L Inoculum holding: None ~ used the day it was obtained Test conditions Dilution water: Millipore Mili-0 water SRyenftehreetnicceSaenwdagtees:t Per OECD method solution preparation: A stock solution of the reference dissolving substance, 500 mg in 3,5-dichlorophenol, 10 mL of 1N NaOH, was prepared by diluted to 30 mL with Mili- Q@ water, then brought to the point of incipient precipitation with 1N CO4288 H2S04, and diluted to 1 L with Mil-Q water. The pH of the reference solution was measured to be 7.3. The test solutions were created by direct addition of the test substance to the test vessels. After test substance addition, the vessels were stirred with a Pyrex pipettefor approximately one Vamfiitngeuortreso.tuisiAnsghw.ahkiTitnehgeprwpeaacristpiidctloaentseewwbeaersfeorsseeuesrnpeemoonnvditenhdegdbeuoartcitnhogmsaaeomrfapttlihoeen.avleisqsueolts for testing. sToelsuttivoenssels: 1200 mL Pyrex FleakersTM containing 500 mL test bNluamnkbecorntorfolcsoncentrations: 3 plus one reference control and two Temperature: 19-21C Total Suspended Solids and pH for sludge on day of testing: 4380 mg/L and 7.87 respectively. Element basis: respiration inhibition as determined by oxygen consumption Method Remarks: Test temperatures were not controlled. The respiration rates were normalized to 20C for the inhibition calculations. a e RESUe LTS e--------------e---- mNgo/mLi)n,a1l00c,on5c0e0n,traantdiodnusp:licBaltaendk1c,o0n0t0roml, grefteersetncmaetseruibasltasonlcueticoonnstrol (10 Element Values: 30-minute ECs, = >1,000 mg/L 3-hour ECs = >1,000 mg/L Element value based on nominal concentrations Analytical Methodology: Analysis of DO concentrations in all test solutions were performed using a dissolved `oxygen meter. Control response: Satisfactory Remarks: No oxygen inhibition seen in the 1,000 mg/L exposure concentration relative to the control in the 3-hour exposure. sCoOnNoCuLoUsSIiOoNnSs C0489 The test substance exhibited onlya slight inhibitory effect (<20%) upon respiration at a nominal test substance concentration of 1 ,000 mg/L at the 30-minute exposure and no inhibitory effect at the 3-hour exposure. The ECs (respiration inhibition) concentration, 1,000 mg/L. is therefore greater than the highest exposure Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 o0 DnAmToAuQnUgALoIyTYY. Reliability: Kiimisch ranking 2. Testing meets all criteria for quality tTehsetrineg,isbautlalcakckosf acnhaalryatcitcearlizcaotnifoinrmoaftitohne otfesttesstamspulbes.tance`concentrations. RReEFFeERREENCESS This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request P3025 oOTeHER. Last changed: 5/25/00 94290 3M ENVIRONMENTAL LABORATORY INHIBITORY EFFECTS OF P3025 ON ACTIVATED SLUDGE RESPIRATION OECD Activated Sludge Respiration Inhibition Test, #209 STUDY COMPLETED: January 21, 1887 FINAL REPORT COMPLETED: May 12, 1897 Env. Lab Request Number P3025 NaPrmeep:ared by: Title: Laboratory: Address: ASduvsanacAne.dBEenavcihronmental 3BiMoloEgnivsitronmental BLuaiblodriantgor2y-3E-09 9St3P5auBlu,shMNAv5e5n1u4e4 04r9L PAcatgieva1ted Sludge Respiration Inibiion May 12, 1997 wTihteh hpuer"pAocstiovfattehdisSsituuddgyewRaessptioraetviaolnuaItnenbtihteioinnhTiebsiStto"Ur,yMOeMfEfACeRcOtYoNfo.P320020.5 oTnhaeemroebdiicanskaufdfgeectsiyvestceonmcseniniraactcioonr,dance Pour ECg was reported as > 1000 moi. fToercitcstyoftepsottsewnititahlaceornotbaicmisnlaundtgse opnrotvhiedeopaerraatpiidoInsNcorfTeRaecOntiDinvUgaCtmTeedIlsOhlNouddgaentdraeraeimeelnitbslyestpermedsi.ctors of the inhibitory t"mTriheniasutttmoeexsnicttiotsy3ys-sthtoueudmrysam)nediansftuohlreleopdwretedhsedeenrcecsrepeioarfsaetdiisofInfnerrtaethnete ocrfoatnaeccetoinfvtoarxtaeytdigoesninsucdoogfnePso3ub0mtp3at5iineodn ofvreormaa mreulnaitciiveplaylswhaosrttepweartieord (30- TestSubstance: Appearance `Aqueous Solubilty APvraopielratbilees:Physicochemical Date Received Storage Test Solutions Reported values MATEARNDIMEATHLODSS P3025, date 10/7186, Lot 1 Two-phase dispersion (lear liquid wih opaque sold) Apparently not completely soluble in water a tested concentrations. VaBopiloirngPrPeoisnstu:re: EVvaappoorraDteinosniRyate: SSpoelcbiiflilcyGrianvwiattye:r: pPHe:rcent Volatle: 10116198 c1a,0018Cmm@H20gC <p1i cian.so1l:u2ble 66-08-70% In the dark at ambient room temperature (22 + 2C) `sToeunsbetsmtsiaunnbuctseet,aadndcTieetsiwtoens,iugvbhessetsdaenolucstewafenordremseaiddrdweehddilwdeiilrpheacratilcPyiyterosetxwehsptiipcvehetstSseealnsok.r(0aA(pfhpeerrobtxoeitsmttaotmeloyf the FleakerTM. Particles were suspended during aeration bTehgeinrneipnogrtoefdtvhaelmuiecsraobriealbaassesdayon nominal concentrations in the test media al the Canyrols: Functional Control (Reference substance) 3Al.d5r-idcihchrleodrolapbheeln;o9l7%(.3,5-LDoCtP)0.8615KG DQ. Received 11/18/96, 04292 APcatgieva2ted Sludge Respiration Inhibition May 12, 1997 Controls(Cont): (uBnltarnekactoendt)rol Trewfoerceonncteroclhsemciocnatlasin,iongneinatoctuhleusmtaarntdannduttriheentotmheedriautmt,hebuetndnooftetshteotrest series. InoculumNutdentMixture: Microbial Inoculum Nutrient Medium 4ATdr3te8iavtamt.een0dt7s2lPluagdnLgte;, mStiinxiPteaiduallli,pqHuMoi7rn.wn6.eassTothoaebtosanliutndeegsdetfwdraaoysm. ctThoenhteMienstuurosouppsoellynidtaeaednraWstoaelsditdaesfwtleaeritvetelrwwasa.s received in the laboratory. `Synthetic sewage feed prepared on test day as outlined in OECD 209. Exposure: Test Date Contact Time Vessels Test Solution Vol. Starting Test Conc. "Test Substance Reference Substance Mixed Solids LLeivqeulorinSTuesspteMneddeida Dilution Water Temperature Lighting Mixing and Aeration 113/97 `30-minutes and 3-hours. 1200 mL Pyrex FleakersTM 500 mL 100 500 1000 mg/L (in duplicate at 30-min; 3-hour duplicate lost due to lab accident) 10 mg/L. 175 gL MilliporeMill-Qwater 15.6 - 18.0C over the course of the study Ambient laboratory light Test solutions were continually aerated with clean, oil-free air. Measurements tBDiiOmseDs.olbAovtestdlueobxa-ysnagdmedpnilscesooonflcvetenedtstroasxtoyilgoutneisnownceowrnaecsenmqteuraiacstkuilroynepsdowuaefrtreeedr f3rr0eo-cmmoirtndhueedteeusxspiaonnsgdura3e-dhivoseussrosslevlceoidnnttoacat 3W`ho0ix-lymegienrnuetcmeoertdreeirandgeivnoegxrsy.yge3n0.-sTechoensdusbo-vsearmaplfeivwea-smirneuttuerpneerdiotdo. thTehmeasiunbt-essatmspolleutwioansafsttierrretdhe 04293 APcatgieva3ted Sludge Respiration Inibition May 12, 1997 Procedure TInhheibtiteisotnwTaesstc",onOdEucCtDedNoi.n g2e0n9er(a1)l. aVcaclouredsanwceerewirtehptohrete"dAcotidviaftfeedreSnltuedvgeelsReosfpiration psirgonciefsisc.ance depending on the accuracy ofthe measuring devices involved in any one CALCULATIONS cTohnecernetproarttieodnsvatleusetsedaraendbatsheedcoornrnesopmoinndailncgonrceesnptornasteiodnastia dtehrivteesdtfmreodmitahaettotxhiecibteygtiensrtianrgeoufstehde tteostc.alcTuhleate, if proesgsriebslsei,onthmeemtehdoadsl (eif.f,ectPirvoebictonAcneanltyrsaitsi,oTnr,iEmCmsegd Sapnedatrhmean9K5a%rbceorn)f.ideTnhcee iEntCersvails bdyefsitnaenddaarsdtlhoegicsotniccentration eexstpiomsautreedttoo tinhheibtietstthseubrastteanocfer.espiration by 50% as compared to the corirols, after 30-minutes and 3-hours of RESULTS `mTihneutpeesrcaenntd r3e-dhuocutrisonexinporseuspriera0titonheratteestasnudbsttheanmceedPi3a0n2e5ffaercteivseumcomnacreinzireadtiionnT,aEblCeso1., oTbhteaipneerdceanftterre3d0u-ction in respiration rate of the reference substance 3,5-Dichloropherol is also reported in Table 1 `"TThhee m9e5d%iaCnoenfffiedceinvceecIonntceernvtarlactoiuolnd, n3o-thobuercEalCcuslatofedP.3025 10 activated sludge was determined 2s 1000 mg. ACCEPTABILITY CAITERIA This toxicity test meets quality ceria provided in test guidelines: 1) Tehxepotsuwroe.blaTnhkecopnetrrcoelnrtesdpiifreartieonncerastweesrweer2e2.w2itahinnd145.1pearlcteenrt3o0f-emaicnhutoethaerndaf3e-rho3u-rhsouorfs exposure, respecively. 2) TWhaes3w-ihtohiunrhpeercneonrtmailnhriabintgieonsienednuwcietdh bhysthmeixreedfelrieqnuocreisnutbhsistalnacboer,at=o3ry5-(s06CePT,able 2) 3) Tbhegeinrneiponrgtoefdtvhaelmuiecsrowbeirael absassaye.d on nominal concentrations in the test media 21 the This test was conducted by Trudy K. Stiefenhoeter as requested by Pam Wolf of 3M SCD Division. BEFERENCES `1C.hemicalOs.ECODrg(a1n9i8z4a).tioAnctfoirvaEtceodnSolmuidcgeCoRoepsepriartaitoinonaInndhibDietvieolnoTpemse,ntT,esPtarNoi., 2F0r9an.ceI.n: Guidelines for Testing of f2o.r TestiOngEoCfDChe1m9i8c1a.ls.PriOncrigpalensiozfaGtoiofoodnLEacboonraotmoircyCPoroacpteircaei,iAonnnaensd2D,eCv(e8l1o)p3m0en(tFi,naP)a:rs7,28F.ranOceE,CD Guidelines 04294 APMcaatygive1as2t,ed1S9l8u7dge Respiration Ihisiton TABLE 1 SUMMARY OF TOXICITY OF P3025 TO ACTIVATED SLUDGE --_--PEACENTINHIBTIONINRESPIRATIONRATE TEST CONC. mg/L. 30-min. Exposure hours Exposure w wo v 500 o 1000 17 0 1000 (dup) 1s a-ha ECs0 95% Confidence Inerval Salifcalmethodused >1000 xine woscolae >1000 retin Noscokwe SUMMARY OF TOXICITY OF 3A,C5T-IDVIACTHELDOROSPLHUEDNGOLE REFERENCE SUBSTANCE TO TESTCONG. mg/L 10 PERCENT INHIBITION IN RESPIRATION BATE 20-min,Exposure :hoursExposure 36 54 C0495 APcatgiveasted Siucge Respiration nhviton ~~ __TABLE 2 Siizisr eco 209 Results from Reference Toxicant Testing with 3,5-Dichiorophenol Test Concentration = 10 mgiL PercentInhibitioninOxygen UptakeRate Ta11t/2T7o9s5a 30min 3Exposure as E5x5posure WS5Ss oT ZREESEs |[ Ts5 e | [Comes 12 5 ss 5 5] ] 5] [Cmosmmmssee[ TT T Ts5 e2T 1 0 e% r115 555575]] [[mmesms ses T [TT f s s e| | Te e se| | --4357--]] 55] [eroSesde Bess 1 [ [s ss s s[ I T 55e s 5] 55--] mRseesss [Comoe |T [wa e 5| |Te w er T] T 5s7o1]] SSeoses Smose | | e a0[T sr s 3 57 [se as 55 oTsoesss[Taead[T ei T s a3y5] a TomZSeRE |a ] s1I s] 130] [[roesmises 1 TT% ss ress TT so [ TT 5 | TT 345s]] TT 6 47] [meTTEeSssEsS T |[ T[s2o | s s Ts ws ; 5; [mer er iss Ts ws 5 e e5 33 55 5] ] [s Te +] or Tr s1 r ss ai] [MSEengAarsNbevaied T | v03 | e 7s5s ] r | a | oss] "Mixed iquor suspended sos MetaWastewater Treatment Pian, St Paul, MN 04296