Document 37NEe95V9oa7NBZmrJYqe797y

A SLSL& - 0 9 2 3 MICROBIOS MICROTOX TO XICITY TEST TEST SUBSTANCE______________ . . Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3106. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 015G. The test sample is FX-3106, PO# S5233-004, EAI 85548, Sumitomo 3M Japan. Current information indicates it is a mixture of 2.31% PFOS, 2.6% diethylene glycol butyl ether, 83.38% water, 10% ethyleneglycol, 1.36% Sultone foamer, 0.2% xanthan gum, 0.1% 2-biphenylol, sodium salt, tetrahydrate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: Microbios Microtox "BASIC" Procedure T est type: Acute static GLP: No Y ear Com pleted: 1985 Species: Photobacterium phosphoreum A nalytical m onitoring: pH and osmotic condition. Exposure period: 15-minutes S tatistical m ethods: EC50 values calculated by statistical linear regression program, e.g., probit. T est organism source: Microbics' Reagent. T est conditions: Diluent: Millipore Milli-QTM water Test tem perature: Not noted. Stock and te st solution preparation: Not given. Exposure vessels: 4 mL cuvettes. Num ber o f replicates: two Num ber o f concentrations: Four plus a blank control Elem ent basis: Percent light loss RESULTS________________________________________________________ Nom inal concentrations: Blank control, 10,100, 500, and 1000 mg/L Element value: 5-minute EC50 = >1000 mg/L 15-minute EC50 = >1000 mg/L 003020 Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_____________________________ ______________________ The test substance 15-minute EC50 for Photobacterium phosphoreum was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY_______________________________ ____________________ R eliability: Klimisch ranking 3. Study lacks description and records of the method followed. There is no characterization of the test substance purity, and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C 2 2 8 2 ,1985. OTHER________________________________________________ :__________ Last changed: 6/26/00. 0030rfi ENVIRONMENTAL LABORATORY BECKMAN'S MICROTOXR SYSTEM MICROTOXR RAW DATA LR S TEST SUBSTANCE:F X -3 \O l> P o S 2 Z 3 - Q Q H B A X S S S U TEST DATE: O ' " 8 5 ~ ~ ________________________ S u r ^ o ^ o 3M J V p a n (U * d i5 ( SAMPLE CUVETTE BI Cl B2 C2 B3 C3 BA C4 B5 C5 FINAL ASSAY CONCENTRATION BLANK CONTROL DUPLICATE IO v n & lZ bopli'ccjdte. )0 o t^uplcCt ^wph'cdci^ 1o o o i/vi^ INITIAL READING KO) 96 FINAL READINGS 5-MIN. 15-MIH. 30-MIN K5) 105) K30) S3 S O 87 8| 7 3H 8 l S 3 7 T 7^T `7 8 7 ^ 4 9 84 So S7 so 81 78 44 7/ "7 75" 4^ A 0) ss' 51 00 3022 R BECKMAN'S MICROTOX*1 SYSTEM R fO microtoxr data reduction /O-3^8 ' S S T F X - 3 IO> P * s 2 3 3 -0 0 r X % S S ^ 8 i Final BLANK RATIOS* Son\itowo 3M-T<xp<xr\ (Loi' Oi.S'Cr^ Initial ------- 5 = H T W. ------- n = H ! T ---------- ------- J O - B I N . ---------- BLANK CUVETTE BLANK RATIO DIFFERENCE BLANK RATIO DIFFERENCE BLANK RATIC DIFFERENCE B1 h o -o.^ua. O . C c ^ */lO = 0. SSH 0008 Cl a,/i7 - O. *?3 1 Vd/ J7 .r _ 9 ,\ $ 1 7 - *The blank ratios for B1 and Cl should agree with 0.02. MEAN BLANK RATIOS B1 BK RATIO + Cl BK RATIO 2 5--MIN. o.<U7 15-MIN. eS1 ______ 3Q-MN - .-,. ,, __ Mean Blank Ratio x Initial Saaple Reading -1 Final Sample Reading SAMPLE CUVETTE H l o m a j iX . " to o r n & l- l 5 S c g i . ( cs / c o o 0 f t I X 0* 0 00 *> 0 5-MIN.r - 0 .0 18 " O-OAfc 0 . a 4 0 . i31, 0.301 0 .A T 3 15-MIN.r - O - 01*1 " 0. C 131 0 .00 c 0 .o a 1 <5. 551 ~ 6 . ia<* 0 . z\<\ 30-MIN. r ' Z LIGHT LOSS ( X A ) - -- ----X 100 1+r SAMPLE CUVETTE ll t O rrtftj-l " 1 0 O " A IX . It S C O 0 f t J "5 l O O O n U 5-MIN. Z A -- o.*t A. A II ia A3 EFFECTIVE CONCENTRATIONS (EC's) MG/L 15-MIN. Z A A.3 l3 11 3 ____ J L 1 _________________ 30-MIN. Z A EC'S 5-MIN. 15-MIN. EC20 77^ SIS ECcn iV^Z Con fid. Limits), \ 50 ECftft I7 C 857-- 3 U H I ) 80 * o c . ? __________________________ JLX ?>S > lo S (iz \i~ 3 iB i) 30-MIN. 003023