Document 37B8bVaX5kyDD6Q89LyGEZZj0

STUDY SUMMARY OF ENVIRONMENTAL AFFF AND COMPONENTS - 1991 EFFECTS OF LIGHT WATER BRAND TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component ofFC-203. (1Octanesulfonicac7id()CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-203 withouttolyltriazoildee,ntifieads VW160390. Currentinformation indicatesitisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether, 37.9% water,20% ethyleneglycol,2.66 % Sultonefoamer,3% sodium octyl sulfate,and 0.1% sodium laurylsulfate. The following summary applies to a mixture with incompletely charactedzed concentrations of impudties. Data may not accurately retlecttoxicityor degradation of the fluorochemical component of the testsample. RESULTS The VW160390 48-hr EC5o forDaphnia magna = 165 mg/L. DATA QUALITY Reliability:Klimishranking= 4. Value isfrom a summary lisotnly. OTHER Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 Last changed: 6/28/00 T;chnicalReportSum'mary To.-Patent& TechnicalCommunications Services- 201-2C-12 RepoilSummary must be typewritteGnu.idelineasreon reverseside.IfreportIsprintedon bothsidesofpaper,sendtwo copiestoP & TCS. Dwument7r ENi;u@ummber, 5;parWMt Number 0222 To Pike RT- iiowts) Ho@ell RD Pmod Number I P"W Number I Date TypW 4-1-91 Emp" N~s) 295864 cc U)C OrWU&tlon cede (Pla= additlonm D.Ns In Abstnwt w*Q Fnv4rnnment,al PMIW Dowriptlon Engine-exing & Pollution Control 1! 0 1 -082 EE&PC VPP Rpnparrh Rowyloo and I)Pvalopment pedw C*VWW -@-EnviroTmental Effects of LIGHT WATER Brand AFFF and Components Keywords UGHT WATER, aqueous foam, FC-20@, FC-206, ethyleneglycolb,utylCarbftols.odium lauryisuffatDeu,poriol,sodium dodecylsuffatseo,dium octylsugateL,-4640,triethanolamineu,rea,Tdton X-305, foamer,Emer, surfactardt,olykdazole, environmentaleffectst,oxicityD,aphnia toxicityM,icrotox.algaltoxicityf,ishtoxkftyd.egradabliftbyl,ocherrdeaolxygen demand, BOD, chemicaloxygen demand, COD. ProjectObjective& ReportAbstract i.To add totheknowledge base of environmentaleffectsofLIGHT WATER brand aqueous filmformingfoams and the components used intheproducts.2.To identiftyoxiccomponents and to correlatecomponent and producttoxickles. Thistechnicalreportincludesa summary of environmerdaleffectsdata on 3M's productsand thecomponents used In them.The datawas takenfrom theliteratuarned from previousstudiesby the 3M EnvironmentalLaboratoryT.he data foreach component and each producthave been evaluatedforcor*eteness and accuracyand additionatlesting requirementshave been noted.Where suffwieridtata on thetoxicitaynd degradabflftoyfIrKrividucaolmponents were availablet,hedatawere evaluatedand recomniendationsregardingWusion of thecomponent infutureproductshave been made. An attemptwas made tocorrelattehetoxicitoyf FO-203 tothe toxicitoyf itscomponents by takingirdoc@onsideratiotnhe component concentratioinntheproduct.While producttoxicitcyouldnot be conclusivelcyorrelatetdothetoxicitaynd concentratioonfindividuaclo nents,majorcordnbutorsto producttoxicitayre believedto be thefilnietrh,efoamer, butylcarbkoffa,nd DuponolTP.MO,E (sodiumlaurylsuffatCeo)m.ponents likeltyocontnbtaelesstoproducttoxicitayre ethyleneglycolt,olyftdazolseo,dium oetylsulwo,and Di-water.Furthertoxicittyestingofcomponents and product fomvilauonsshouldenablebettercorrelatiobnetween coniponerdand producttoxk-Ales. ReportT ype I Security Notebook Ratenmee :91 R & D Research and Development PILotPlant MANufacturing Management SUMmary El Onan R8Dort and Summary informatlonUslaw lnltws 0 TRP Tripor FieldReport 0 FACtory Experiment El ENGineering 0 ROI Record ofInvention 0 TECH. Service 0 Govt. Project 0 OTHER 0 Closed Report-Open Summary 3M Chemical 0 New Chemicals Reported Registry Un form6M toenterIntoChemkW Paostry For P & TCS UseOnly Nun*erofPsoa P & TCSEditorinitials Contents 1.Purpose .............................................................2.................. 11.Backgmund .................i...................*...........................................2................. 111G.eneralComments ...................................................4................ IV.Co'nclusionasnd Recommendations .....................................6................. V. EnvironmentalEffectUsteratureSearch ...................................9................ 1.EthyleneGlycol(RM 3017) ..........................................1.0................ 2.ButylCarbito(lRM 8887) .............................................1.2................ 3.Duponol(RM 3021);DuponolME DrySurfactan(tSodiuniLaurylSUlfate)......1.4. 4.Tolyltdazol(eRM 27513;CorrosionInhibitToorlyftdazole.TechniGcralde) .....1.6 5.Foamer (FM-4115)..................................................1.8................ 6.Filmer(FM-3820orFC-95) ..........................................1.8................ 7.Na-octylsulfa(tReM 32067 orRM 55040) ...............................1.9.,............... 8.Di-water(RD 199) .................................................2.0............... 9.FC-203 ...........................................................2.1............... 10.VW1 60390 (FC-203withoutTolyltdazolReM 27513) ..................2.3.............. 11.FC-203CE .......................................................2.4............... 12.FC-206CE .......................................................2.5................ 13.FC,203CF .......................................................2.6................ 14.FC,206CF .....................................................................2.6............... 15.L-4640(New Foamer) .............................................2.6............... 16.Witcolat7e093 (RM 45666)..........................................2.7............... 17.Tdethanolamine..................................................2.7............... 18.Urea ...........................................................2.9............... 19.TritonX-305 .....................................................3.0............... EnvironmentaElffectosfLIGHTWATERTU Aqueous FilmFormingFoam an.dComponents 1.Purpose Thepurposeofthisstudyistoincreas3eM'sknowledgoeftheenvironmenteaflfecotfs LIGHT WATEFff aqueous filmformingfoam and the individuaclomponents used tomake the product.And, by understandingthese effectst,ominimizethem throughformulationchanges. li.Background This studywas undertakeninresponsetotestingdone by the German Army which was reportedin"InvestigatioonfsToxicEffectsand BiologicaDlegmdabilityofFoam Extinguishing Agents inWastewatern by Ekkehard IsingT.he report,hereafterreferredtoas the "German Army Report",includeddata for16 firefightinmgaterialso,fwhich two were 3M's FC-203 and FC-206. The 16 firextinguishinaggentswere categorizedas: 5 multi-rangfeoam agents -6film-forminfgoam agents 3 proteinfoam agents 1 film-forminfgluodne-proteifnoam agent I specialfoam agent fordeep-frypan fires EnvironmentaleffectsconsideredintheGerman Army Report-were: 1.Acute toxicittyothe marinephotoluminescentbacterium Photobactailum phosphoreum usingthe Microtox"system.The toxiditwyas expressed as FC.,,and EC50,however,the exposure timeisnotclearfrom the German Army Repb*fltAnd may have been 10,20,or30 minutes.Two multi-rangfeoam agentswere considered "highlytoxic"T.hree multi-rangfeoam agents and 2 filmformingagents were found to be "toxicrF.our film-forminagl,l3 proteint,he film4ormingfluorine-proteaignent,and thedeep-fryagent were consideredto have "lowtoxicityF"P.-203 and FC-206 were inthe "lowtoxicitcya"tegory. 2.Algae cellmultiplicatiionnhibititoenstusing Scenedesmus subspicatusas thetest organism withan exposuretimeof72 hours.Toxicitywas expressed as EC 10and EC50.Allfoam agentswiththeexceptionof two ofthe proteinfoam agents were "toxict"oalgae.The two proteinfoam agents had weak toxicittyo algae.Both 3M productswere considered"toxict"o algae. 3.*Acute toxicittyo Daphnia magna afterexposure for24 hours.The toxicitwyas expressed as LC,o and LC50,butthe effectcriteriownas theorganisms'lossofability to swim and willbe referretdo as EC 10and EC5. inthisreport.All5 multi-rangefoam agents,allfilm4ormingagentsexceptFC-206, and the deep-fryagent were "toxieto -2- DaphniaO.ne film-formaignegnt(FC-206)a,ltlhreeproteiangentsa,ndthe film-formfilnugo6ne-protaegienthad"lowtoxicittorDaphniaF.C-203:"toxid"; FC-206: "lowtoxicity". 4.Acute toxicittyoZebrafish(Brachydanioredo)afterexposure for48 hours.The criteriwaas death and thetox7ic7wiatsy expressed as LC 10and LCro.Only two mult-rangefoam agentswere "toxietofish.Three multi-rangfeoam agentsand two film4ormingagentswere "borde6inetoxictwealdtyo)Cie".Wealdy toxiewere 4 film-forminaggents,2 proteinfoam agents,and the deep-fryagent.One film-forming agent and thefilm-forminfgluorine-proteaignent were "non-toxid.FC-203 and FC-206 were inthe "weaklytoxie category. S..Chemical Oxygen Demand (COD) and 5-dayBiochemicalOxygen Demand (BOD 5) ofa 3% solutionofthetestmaterialinwater.The ratioofBOD .toCOD was used as a ratingguidelinew,itha BOD,,/COD ratioof0.5 orlessconsideredminsufficient biochemicaldegradation"T.wo mufti-rangeagents and'one film-forminaggent (FC-206)were consideredinsufficiendtelgyraded biochemicallyT.he ratioforFC-206 was 0.35.One mulfi-rangaegent and one filmformingagent (FC-203)were considered"probablyinsuffidienbte"cause theirBOD WCOD ratiowsere 0.55.The 9 remainingagents were allsufficienwtith-theiBrOD /COD ratiosrangingfrom 0.60to 0.89. The data from the German Army Reportforthe two 3M.products aresummarized inthetable below.-The-data forthe Microtox,Zebrafish,COD, and BOD ,testsseem to be "reasonable* and "self-consistenTth"e. data are consideredreasonablebecause they agree fairlwyell%(ith data from previous3M studiesand are consideredself-consistebnetcause the FC-206 data are roughlyhalfthatofFC-203. Summary ofGerman Army Report Data Prowet FC-2D3 wffotox EC,, EC, 632 2,500 -LDWTOX;W Aigae EC, Er,,, 16.9 160 'To)de. Daphtia Lei* LC, 31.6-, 430 'Toidcr Zabraftsh COD BODs BODOICOD Less LCN Rado 1.053 1,634 -WealdyTo)(e 41,200 - 21,500 - 0.52 P"mbacmwyr FC-2D6 2,040 15,000 -ww Toxicity 16.3 160 ITO)dcr Nole:allconoontragonsinmgll. 305 2.000 'Low Toxicity' 2,036 4,086 -WeaklyToxW 24.6w 8,700 - 0.35 'Insuffidenr -3- For the algaltoxicitdyata,thereare two importantpointstoconsider.Firstt,he German Army's algaedatashow essentialleyquivalentoxicitfyorour two products.One would expect more toxicitfyorFC-203 than forFC-206, as shown inthe Microtox,Zebrafisha,nd Daphnia tests.However, highertoxicitbyy FC-203 than by FC-206 may notalways be true.For example,theremay be a component which isparticulartloyxictoalgae,perhaps disrupting the'photosynthetipcrocess.Second, our PED forFC-203 indicatesno effectotwo algal speciesatconcentrationusp to 1,000 mg/L.Whilethe two algalspecieson the PED are not thesame as thoseinthe German Army Report,theyare allfreshwaterspeciesand such a largedifferencientoxicitwyouldn'tbe expected.One importantlimitatiwoinththe PED datais thatitdatesfrom 1974 and ithas been impossibletoverifythe exactformulationofthe sample used inthose tests. For the Daphnia data inthe German Army Report,the differencebetween the *FC-206and FO-203 valuesseems largewhen consideringthewater contentofthetwo formulationsA.lso, thereisa consi* derabledifferencbeetween the PED data.andthe German Army data.Our PED has a 48-hrstaticDaphnia magna LC50 of1,600 mg/L, whilethe German Army Report has a 24-hrstaticDaphnia magna EC.50is430 mg/L The PED dataisquiteold (1973)and the formulationofthe testsample cannot be verifiedT.here isa major differencbeetween the PED data and theGerman Army data inthatthe PED data isthe concentrationwhich causes deathto 50% ofthe Daphnia testpopulation(LC 50)and the German Army value isthe concentratiownhich causes 50% ofthetestpopulationtostopswimming (Effective Concentration= EC50).The German Army valueisinfairagreement withrecentresults which show a 48-hrstaticDaphnia magna EC50 of229 mg/L. One thingthatshould be keptinmind when consideringthetoxicitdyata isthatreproducibility and prec7isionftoxicittyestscan varyconsiderablyfrom laboratortyolaboratoryand over time.However, the differencebsetween the olddata (circa1974) and the more recentdata is more likeldyue tochanges informulationratherthan changes intestprotocolor testspecies. At any rate,our primaryinteressthouldbe thetoxicitoyfour currentproductsand WiD.should be concerned withhavingcurrent,reliabldeata on our PEDS. IllG.eneral Comments The followingeneralstatementssummariz.ethe factorsguidingthisstudy. 1.The primarypurpose of.theenvironmentaleffectsdata isforinternaluse inevaluating formulationasnd ingredientasnd notintendedforsubmissiontogovemmental regulatoryauthoritieTsh.erefore,any testingshould be done usinglow-cost, non-GLP methods.An exceptionmay be data forthe new foamer (L-4640)which may need to be submittedforthe European PMN currentluynderway. 1 DraftReport from EnviroSystems Laboratorydated March 10, 1991.We have asked EnviroSystemsto repeatthe Daphnia testsforFC-203 and VW1 60390. The preliminary resultsfrom EnviroSystemson the restofthe samples were acceptable. -4- 2.The German Army performsitsown testingand no authoritoytherthan the German Army ispresentluysingtheevaluationscheme used inthe reportcitedabove. Therefore,thereisno need tochoose a laboratoryt,estpmtocol,ortestspecies applicabloenlytotheGerman Army testscheme. Instead,testmethods and species shouldbe chosen so thattestresultscan be used inas many countriesas poss7ible. 3.Where possibled,ataon theenvironmentaleffectsofcomponents should be obtained from the literatuorrefrom raw materialsuppliersinordertoreduce testingcostsand time. 4.The Natonal InstituotfeOccupationalSafetyand Health(NIOSH) terminologyforthe relativteoxicitoyfthe components and the formulationswillbe used inthe discussionoftoxiceffectsW.hilethese terms were not used inthe German Army Report,theyare generallayccepted inthe US. The toxicitaynd correspondingterms are: ConcentrationAcutely ToxictoTest Population NIOSH Terminology <1 mg/L 1 - 10 mg/L 10 - 100 mg/L 100 - 1,000 mg/L >1,000.mg/L NHighlyToxicr "ModeratelyToxie "SlightlTyoxie "PracticallNyon-Toxid" "InsignificaTnotxicity" 5.The LIGHT WATER AFFF formulationasnd components consideredare: 1.EthyleneGlycol(RM 3017) 2.ButylParbftol("RM 8887) 3.Duponol* ME (RM-3021) 4.Tol*Yhdazole(RM 27513) 5.Foamer (FM-4115). 6.Filmer(FM-3820 FC-95) 7.Na-octylsulfa(tReM 32067 or RM 55040) 8.Di-water(RD 199) 9.FC-203 10.VW1 60390 (FC-203withoutTolyltdazolReM 11.FC-203CE (same as FC-203C) 12. FC,206CE 13. FC-203CF 14. FC-206CF 27513) -5- IS.L-4640 16.Witcolate7093 (RM 45666) 17.Tdethanolamine 18. Urea 19.Tdton X-305 Items1 to 10 are from a lisgtiventoEricReinerby Wim Vanneste 3/12/90.Items11 to 13 were giventothe authorby Wim Vanneste 2/5/91.Items14 to 16 were given tothe authorby Roger Altn(2/4/91). IV.Conclusions and Recommendations Inordertocorrelattehetoxicitoyfthe producttothetoxicitoyfindividuaclomponents,the component concentratioinstakenintoconsiderationI.nthetablebelow,thetoxicittyo Daphnia ofthe pure components are giveninthethirdccilumn.The fiftcholumn contains estimatedtox@idifbiaesed on productformulationT.hisisthe toxicitwyhich would be expected when the component isdilutedinwatertothe same concentrationas inthe product.For example, one would expectthatsincethe toxicitoyfpure butyl*6arbftoli'sOabout 700 mg/L, thetoxicitoyfa solutionmade of35% butylCarbitoloand 65% waterwould have a toxicitoyf about 2,000. Some interestinogbservationscan be made from these numbers. Firste,ven though tolyftdazoliesquitetoxic,itispresentatlow concentrationinthe productand when dilutedin the productitprobablydoes notcontributseubstantialtloy producttoxicityH.owever, this analysisdoes notexoneratetolyltdazolaes a majorcontributotroproducttoxicityS.econd, the lowestnumbers inthe fiftcholumn indicatecomponents which willbe the most likely contributortsoproducttoxic7ibtuyt:ylCarbitol'Dou,ponol'PME (dodecylsulfatet)h,efoamer,and thefilme@Urn.fortunatelyt,hereisnota singlecomponent which standsoutWitha n6mber in thefiftcholumn which issubstantiallyowerthan allthe rest.This may mean thatt6@r.i*sea synergistiecffectbetween components. iton Nania E(P%W3rIXYCOI Summary ofRecentDaphniaToxiciDtayta 9ri6T=Wl= >1,000 FC-2D3 Formuladon 20.00% CoffoonoMTamft Bond an Fomwkdbn >5,000 2. ButylCarbtbl* (RM 8887) 3. Duponol*ME (RM 3021) 4. To_MWamole (RM 27513) S. Fowner M"l 15) 6. FHffier (FM-W2O FC-95) 7. Na-octylstdfate (RM 32057 orRM 55040) S. Di-water (RD 199) 9. FO-203 10. VW160390 (FC-203 wiftutTotyl"=Ie) 706 (523-1,000) 2.9(2.3- &5) 19(12-25) 68(50-81) 49(43-56) 517(400-1,000) >1.000 229 (150-444) 165(128-248) 35.00% 0.10% 0.05% 2.6S% 1.34% 3.00% 37.85% 2,017 2,900 38.000 2rW 3,667 17,= >2,642 ,MFt!g@"@F'tor? Probablynot Probably Probably Probablynot Probably Probably Probablynot No x x The importanctonclusiofnromtheseDaphniaresultissthata more completesetoftoxicity data isneeded, especiallfyoralgae.The tablebelow summarizes the additionadlata needed tocomplete the environmentaleffectsdatabase on our productsand theiringredients. Additiondtestsare indicatedwithan X. 2 Preliminaryresultsfrom EnviroSystems,March 10,1991. -7- Summary ofAdditionaTlesting hmn Narm Lsoratox AJgae Daphnia Fish COD BOD I.- E"ne glycol (FTAA3017) 2. Bu ICarbitD]* x x 3. Duponor ME (RM 3021) x x 4. Tolyltriazole (RM 27513) x x 5. Founer (FM.4116) x x 6. Fiknor x x (FM-= - FO-95) 7. Na-o*lsultate x x (RM 32067orRM 55040) S. Di-water (RD 199) 9. F@-2D3 x 10. VW160390 (FO-2D3 x x *ithoutTo@yltiazole) ii. FC-2D30E (sameas FC-2D30) x x .12. FC-2DSCE x x 13. FC-203CF x x x 14. FC-206CF x x x is. L-" x x is. VVhWate 7093 (RM4%W) x x x 17. Triodmmkvydne is. Urea .19. TritDn)@-W5 x x x TOTAL 12 is 6 Note:BOD tealngwillinclud5e-,1o-.and 20-day*str.. x x x x x x x x x x x x x x x x x x x x x x x x x 7 8 10 V.EnvironmentaElffectLsiteratuSreearch Datafrompreviou3sM EnvironmentLaalboratoSrtyudieasndfromtheliterataurree summarized inthissection.Toxicitydata noted by "AQUIRE" were obtainedfrom a search of theUSEPA AQUIRE database on Feb. 12,1991. Includedas partofthe referenceforthe datafrom AQUIRE are the date ofpublicatioonfthe originajlournalarticlaend the review code establishedby a group ofEPA reviewers.The reviewershave ranked toxicitdyata from the literatubrye thefoilo%(insgcale: 1. Meets allreviewcriteria. 2. Procedures generallysatisfactory. 3.Insufficieinntsome area ofthe testmethodology ortestreport. 4.Abstractorforeignpaper %(dhdata inlimitedformat. The complete contentsofthe AQUIRE database foreach chemicalisnotgiveninthisreport because thedatabase includesmany obscure spec7iesof*littilneteres(topossum shrimp, Indiancatfishs,nakehead catfisho,ysters,clams,sponges and many others)and datawhich isconsideredby the EPA reviewersto be ofquestionablevalue,. Additionadlegradabilitaynd toxicitdyata have been taken from the Handbook of EnvironmentalData on Organic Chemicals by KarelVerschueren (NostrandReinhold,New York,1983).These are referencedby page number inthe Handbook followedby the dateof the originarleferenceT.here are many cases inwhich the same data appeared on AQUIRE and inVerschueren but onlytheAQUIRE referenceisgivenbecause itincludesthe EPA qualityratingofthe originarleference. Raw materialsupplierswere identifiefdrom the 3M PRISM database and supplierswere contactedand asked forany environmentaldata they have. Unfortunatelye,nvironmentaldata was notgenerallyavailablferom raw materialsuppliersA.nd, the supplierswere slow in sendingthe limitedata do have. The source and reliabiloifttyhe literatudraetawas taken intoconsideratioinnthe environmentalassessment ofthe components and the recommendations forfurthertesting. Repgrt Format: Informatiofnoreach component orformulationisorganizedas follows: Formulationorcomponent name and RM number Chemical AbstractsService(CAS) Number Vendors Degradabilitdyata from the literatuorrefrom previous3M assessments AquaticToxicitydatafrom the literatuorrefrom previous3M amessments EnvironmentalAssessment Recommendations forAdditionaTlesting 9- Detailedinformatiofnorcomponents and formulationasre giveninthesame orderas inthe lisatbove. 1. Ethylene Glycol (RM 3017) CAS # 107-21-1 Vendors:Chemtech, Ashland Chemical,Dow Chemical,Texaco Chemical,Olin,ShellOil, Union Carbide,TextileChemical,Ashland Chemical, Unocal -Degmdabolitys TheoreticaOlxygen Demand (THOD) BODs BODIO BOD,s BOD20 COD BOD5/COD Ratio BOD2dCOD Ratio 1.29g/g(Verschuerenp 646) 0.440.22g/g (avgof 12 citations from Verschuerenp 646) 0.52g/g(Env.Lab.3 Req. #1940) 0.67 g/g (Verschuerenp 646; 1953) 1.19g/g (Env.Lab.Req. #1940) 0.92g/g(Verschuerenp 646; 1953) 1.14g/g (Env.Lab.Req. #1940) 1.000.08g/g (avgof3 ditafions from Ve rschuerenp 646) 1.02g/g(E'nv.Lab.Req. #1940) 1.240.04.g/g(avgof3 ditafions frbm Vei,@chuerenp 646) 1.28g/g (Env.Lab Req. #1940j 0.4(Env.Lab Req. #1940) 0.8(Env.Lab Req. #1940) Toxicily4e Green Algae (Sconedesmus quadticauda) 7-day StaticPopul.Growth Inhib.5 8-day staticPopul.Growth Inhib. 10,000mg/L (AQUIRE 2;1980) 10,000mg/L (AQU IRE 2;1978) 3 3M EnvironmentalLab work done inDec. 1973. 4 The toxicitdyata are a partiallistinogf38 testson 16 speciesfrom the EPA AQUIRE database. 5 PopulationGrowth InhibitioCnh:ange incellnumber ofalgalspeciesincluding pre-exponentiallag rateeffects. -10- Protozo(aEntosiphsounlcatum) 72-hrStatiPcopulG.rowthlnhib. Blue-greenAlgae (Anacystfsaeruginosa) 8-day StaficPopul.Growth Inhib. Bacteria(Pseudomonas putida) Algae'(Microcysdaseniginosa) Protozoa(Uronema parduczi) Water flea(Daphniamagna) 48-hrStaticEC5. 24-hr StaticLC50 24-hr StaticLCSO 48-hr StaticLC50 Brineshrimp (Artemiasalina) 24-hrStaticLC,,, 24-hrStaticLCso Fathead Minnow (Pimephalespromelas) 24-hr StaticLC50 48-hr StaticLC50 96-hr StaticLC50 96-hr StaticLC50 BluegilSluniish(Lepomismacrochirus) 96-hrStaticLCO Rainbow Trout(Salmo gairdnen) 96-hrStaticLC50 Common Shrimp (Crangoncrangon) 48-hrRenewal LC50 96-hrRenewal LC50 Goldfish(Carassiusauratus) 96-hrStaticLC50 Guppy (Poeciliraeticulata) 7-dayRenewal LC50 10,000mg/L (AQU IRE 2;1980) 2,000 mg/L (AQUIRE 2;1978) >10,000 mg/L (Verschuerenp 647) 2,000 mg/L (Verschuerenp 647) >10,000 mg/L (Verschuerenp 647) >1.000 mg/L (EnviroSystems1991)6 10,000 mg/L (AQU IRE 2;1977) 10,000mg/L (AQUIRE 3;'1983) 10,000 mgAL (AQUIRE 3; 1983) 20,000 mg/L (AQUIRE 2;1974) 20,000 mg/L (AQUIRE 3; 1983) 10,060 mg/L (AQUIRE 3;1983) 10,000 mg/L (AQU IRE 3;1983) 10,000 mg/L (AQU IRE 3; 1983) 49,000 mg/L, 53,000 mg/L, & 57,000 mg/L (AQUIRE 2;1983) 10,000 mg/L (AQUIRE 2; 1968) 41,000mg/L (AQUIRE 2;1960) 100,000 mg/L (AQUIRE 2;1974) 50,000 mg/L (AQUIRE 2; 1974) 5,000 mg/L (AQUIRE 2;1979) 49,300 mg/L (AQUIRE 3;1981) 6 AciAe Daphnid toxicittyestingdone by EnviroSystemsfor3M. Test resultsdated March 10, 1991. EnvironmentalAssessmento Ethyleneglycolhas insignificatnotxicitaynd a high BOD/COD ratioE.ven though itis presentinhighconcentrationinthe product,itprobablydoes notcontributseubstantialtloy producttoxicity. ke@i6mmendations forAdditionalTesfing: None. 2. Butyl CarbitolO(RM 8887) CAS # 112-34-5 (DiethyleneGlycolMonobutyl Ether) -Uhbnyms and vendorseButylDio)(do(lCainChemical),Poly-SolvDB (OlinCorporation), GlycolEtherDB (Oxychem), ButylCarbftolO(UnionCarbideCorporation- Unde Div.), ButylDioxito(lShellOilCompany), Dowanol DB (Dow Chemical),Eidasolve-DB(Eastman Chemical Products,Inc.) Degradabift THOD BOD5 BODIO BOD15 BOD20 COD BOD./COD Ratio BODz/COD Ratio Toxidlyo Bacteria(Pseudomonasputida) 2.17g/g(calculated) 0.25(Verschuerenp 524) 0.69g/g(Env.Lab.7 Req. #1940) 1.45g/g (Env.Lab.Req. #1940) 1.61g/g (Env.Lab.Req. #1940) 1.56g/g (Env.Lab.Req. #1940) 2.08 (Verschuerenp 524) 1.83g/g (Env.Lab. Req. #1940@'. 0.12 (Verschuerenp 524) 0.37 (Env.Lab. Req.#1940) 0.85 (Env.Lab. Req.#1940) 255 mg/L (Verschuerenp 524) 7 3M EnvironmentalLab work done inDec. 1973. 8 The toxicitdyata are a partiallistinogf15 testson 10 speciesfrom the EPA AQUIRE database. -12- GreenAlgae(Scenedesmus quadilcauda) 7-dayStatiPcopulG.rowthlnhib. 8-day StaticPopul.Growth lnhib. 1,000mg/L (AQUIRE 2; 1980) 1,000 mg/L (AQUIRE 2; 1978) Blue-greenAlgae (Anacystiaseniginosa) 8-dayStaticPopul.Growth lnhib. 53 mg/L (AQU IRE 2;1978) Protozoa(Entosiphonsulcatum) 72-hrStaticPopul.Growth Inhib. 73 mg/L (AQUIRE 2-,1980) Protozoa(Uronema parduczi) 420 mg/L (Verschuerenp 524) Protozoa(Chilomonasparameafum) 48-hrPopul.Growth lnhib. 2,774 mg/L (AQUIRE 4;1980) Water Flea(Daphniamagna) 48-hrStaticEC50 24-hr StaticLC.50 706 mg/L (EnViroSystems1991) 2,850 mg/L (AQUIRE 2;1977) BluegilSlunfish(Lepomismacrochirus) 96-hrStaticLC50 1,300 mg/L-(AQUIRE 2; 1977) Goldfish(Carassiusauratus) . 24-hrStatiLcC50 2,700 mg/L (AQUIRE 2;1979) Guppy (Poeciliraeticulata) 7-dayRenewal LC5. 1,150 mg/L (AQUIRE 3; 1981) Silveride(Leuciscusidus) StaticLC50 1,805 mg/L and 2,304mg/L (AouiRE 3;1978) InlandSilversi(dMeenidibaeryllina) 96-hrStatiLcC50 2,000 mg/L'(AQUIRE 2; 1977). EnvimnmeritalAssessment: BOD data shows f*aidyhighdogradabilitTyh.e literaturdeata show thismaterialto have insignificatnotxicittyofishand -Daphnia The two freshwatergreen algae valuesfrom AQUIRE are consistenatnd indicattehat-.butCyalrbito*l ispracticalnloyn-to)(itcogreen algae.There isconsiderablymore toxicitsyhown to blue-greenalgae,however.We should have a sample ofbutylCarbitoltOestedforalgaltoxicittyo be sure ofitstoxiceffects. Recommendations forAdditionaTlesting: Algae and Microtox. -13- 3.DuponolOME DrySurfactan(tRM 3021;Sodium LaurylSulfate) CAS # 151-21-(3SodiumDodecylsulfCaHt3e(CH2)11OS03Na) VO-n-dorDupon? Degradabildy; THOD BOD Toxicily: Green Algae (Chlorellsap) . StaticPopul.Growth lnhib. Green Algae (Chlorellvaulgaris) - 72-hrStaticGrowth Inhibiti12on Green Algae (Scenedesmus quadficauda) 7-dayStaticPopul.Growth lnhib. 8-day Renewal Growth Inhibition FlagellatEeuglenoid(Entosiphonsulcatum) 72-hrStaticPopul.Growth lnhib. Blue-greeAnlgae (Anacystiaseruginosa) 8-dayRenewaJ Popul.Growth lnhib. Bacteria(Pseudomonas pufida) CellMultiplicatiIonnhibition Protozoa(Uronema parduczi)CellMultiplicatiIonnhibition 2.03g/g(calculated) SvAsheeo reportsthat"lineaprrimary alkylsulfateshave long been recognizedas extremelyrapidIn primaryblodegmdao6, often disappearinginlessthan a dar and citesapproximately100 references which show degradationratesof80 to 1000/owithina few hours or days. 50 mg/L (AQU IRE 4;1980) -0.05mg/L (AQUIRE 4) 0.02 mo/L (AQUIRE 2; 1980) 0.02 mg/L (AQUIRE 2; 1978) 40 mg/L (AQUIRE 2;) 7 mg/L (AQUIRE 2;1978) 290 mg/L (Verschuerenp 793;1980) 0.75 mg/L (Verschuerenp 793; 1980) 9 Phone: 302-774-1000.2/15/91:willsend information. 10 Swisher,R. D. SurfactantBiodegradation2.nd ed, Marcel Dekker*New York,1987,p 466, 807-811. 11 The toxicitdyata area partiallistinogfthe 409 testson 49 speciesfrom inthe EPA AQUIRE database. 12 Growth InhibitioMne:asurable change inlengthand/orweight -14- waterflea(Daphnimaagna) 48-hrStatiEcC50 24-hr StaticLC50 24-hr StaticLC50 48-hr StaticLC50 48-hr StaticLC50 .48-hrFlow-throughLC50 2.9mg/L (EnviroSystems1991) 33.0 mg/L (AQUIRE 2; 1977). 7.2.10.0,& 12.0(AQU IRE 3;1983) 7.02.1mgAL (avg of8;AQUIRE 2; 1982) 5.00.5 mgA- (avg of 3;AQUIRE 3; 1983) 1.8mg/L (AQUIRE 2;1981) Fathead Minnow (Pimephatespromelas) 24-hrStaticLCso 48-hr StaticLC50 96-hr StaticLC50 7.4 & 8.5 mg/L (AQUIRE 3;1983) 6.6& 7.3mg/L (AQUIRE 3; 1983) 6.6& 6.9 mg/L (AQUIRE 3; 1983) Bluegil(lLepomismacrochinis) 96-hrFlow-throughLCso 4.5mg/L (AQUIRE 2;1981) Mummichog (Fundulusheteroditus) 96-hrStaticLCso 2.1mg/L (AQUIRE 2) Sheepshead Minnow (Cyprinodonvaileg@,itus) 24-hrStaticLC50 10.0mg/L (AQUIRE 2) 48-hr StaticLC50 9.0mg/L (AQUIRE 2) 96-hrStaticLC5. .9.0mg/L (AQUIRE 2) High-eyesMedaka (Oryziaslagpes) 6-hrRenewal LC50 24-hrRenewal LC50 48-hr Renewal LC50 67 mg/L (AQU IRE 2) 46 mgA. (AQU IRE 2) 46 mg/L (AQUIRE 2) Bdne Sh6mp (Attemiasalina) 24-hrStaticLC50 3.6mg/L (AQUIRE 2;1974) AtlantiScilversid(eMenidiamenidia) .96-hrStaticLC50 2.8mg/L (AGUIRE 2; 1982) iaiiifi(sFhundulus.similis) 24-hrStaticLC50 48-hr StaticLC50 96-hr StaticLC50 4.7mg/L (AQUIRE 2) 4.7mg/L (AQUIRE 2) 4.5mg/L (AQUIRE 2) Ra@in0owTrout(Salmo gairdnen) 48-hrFlow-throughLC50 96-hr Flow-throughLC50 10-day Flow-throughLC50 5.95 mg/L (Verschuerenp 793; 1977) 4.62 mg/L (Verschuerenp 793; 1977) 2.85 mg/L (Verschuerenp 793; 1977) -15- Zebrafis(hBrachydanrieorio) 48-hrFlow-throuLgCh50 96-hrFlow-throughLC50 I0-day Flow-throughLC50 8.81 mg/L (Verschuerenp 793;1977) 7.97 mg/L (Verschuerenp 793;1977) 7.97 mg/L (Verschuerenp 793; 1977) Flagfish(Gordaneliafloddae) 48-hrFlow-throughLCao 96-hrRow-through LC50 10-day Flow-throughLC, 10.0mg/L (Verschuerenp 793; 1977) 8.10 mg/L (Verschuerenp 793; 1977) 6.90 mg/L (Verschuerenp 793;1977) EnvironmentalAssessment: Literaturdeata indicatefsairllyow toxicittyobacteriaand althoughno actualBOD testdata are availablet,hismaterialshouldbe readilydegraded by microorganisms.A BOD value should be obtainedby our own BOD tests. This materialismoderatelytoxictofish,DaphniA and algae.Our most recentDaphnia test data agrees wellwithliteratudraeta which givesus confidenceinthecontractlaboratory's work on thismaterialB.ecause thereissufficiefnitshtoxicitdyatafrom the literaturneo,fish testingiswarranted. Because ofthe algaltoxicitsyhown inthe literatudraeta,algae testingshould be done. Even though thismaterialistoxictoa varietyofaquaticorganisms,itreadilydegrades and adverse effectswillbe minimalifitispassed througha wastewatertreatmentsystem orifa significantumber ofmicroorganismsare presentina naturalwatertodegrade itrapidly. We shoulddo some studiesinwhich our productispasses througha model wastewater treatmentsystem and the measure thetoxiceffectsofthe model'seffluentA.nother study would be a simulationofa naturalbody ofwater using*analuadum stockedwithfish.This would give.usan understandingofthedegradationina riverp,ond,or lake. i4 Recommendations forAdditionaTlesting: BOD, GOD, and Microtox. 4.Tolyltriazol(eRM 27513; Corrosion InhibitorTolyltriazoleTechnical Grade) CAS # 29385-43-1 007H7N3 N N H &10 nyms and vendors: Methyl-IH-Benzotdazole -16- Mothylbenzotdazole Cobratec TT-100 (PMC, Inc.13) PreventolC17-100 (Mobay Corp.14) Dogmdabil BOD Toxiellys Water Flea (Daphnia magna) 48-hr StaticEC50 BluegilSlunfish 96-hr LCao Rainbow Trout 96-hr LCso Fathead Minnow 96-hr LC5. Additionadlata fora similarcompound:. 1H-Benzotdazole (CrH6N3) CAS Number: 95-14-7 Synonyms: 1,2,3-Benzotriazole Aziminobenzene Benzene Azimide Benzotdazole Bluegilslunfish(Lepomis macrochirus) Static24-hrStress Rainbow Trout(Salmo gairdnen) Static24-hrStress Sea Lamprey (Petromyzon matinus) Static24-hrStress Chinook Salmon (Oncorhynchus tshawytsqha) Static24-hrMortality <60% THOD (Mobay Corp.) 19 mg/L (EnviroSystems1991) 31.0 mg/L (Mobay Corp.and PMC) 21.4 mg/L (Mobay Corp.and Pmc) 25.5 mg/L (Mobay Corp.and PMC) o@"I H 5 mgAL (AQUIRE 3; 1957) 5 mgAL,(AGUIRE 3; 1957) 5 mgA- (AQUIRE 3;1957) 10 mgAL (AQU IRE 3;*1969) 13 PMC SpecialitieGsroup, Inc.TechnicalBulleti3n200. Contact:BillMautlewicz (513-242-3300). 14 Mobay Corp.data givenby Matthew Bartman 412-777-2000. -17- Coho Salmon(Oncorhynchkuissutch) Stafi2c4-hrMortality 10 mg/L (AQU IRE 3; 1969) NorthernSquawfish (Ptychocheiluosregonensis) Static24-hrMortality 10.mg/L (AQU IRE 3; 1969) EnvironmentalAssessment: Whilethe limitedata shows thatthismaterialismoderatelytoxic,itispresentatsuch low concentration(0.05,0/i*n)the product,itmay have littelfefectinthe overalltoxicitoyfthe product.Because ofthetoxicitoyfthismaterialw,e need a complete setofdata.There is no reason torepeatthe fishdata,however. Thismaterialislisteodn theTSCA 8(d)lisatnd any testingdone must be reported tothe US EPA. Recommendations forAdditionaTlesting:' BOD, COD, algae,and Microtox. S. Foamer (FM-4115) Toxidily: Water Flea (Daphnia magna) 48-hrStaticEC, 68 mg/L (EnviroSystems1991) EnvironmentalAssessment: The foamer shows slightoxicittyoDaphnia,butthereisno data forthe rema7ining environmentaleffectsW.e shouldhave a completesetofeffectsdata forthismaterial. Care stiouldbe takenthatthe sample used inthe environmentaleffectstesting!$',a-oaod representativseample which has been produced by the manufacturingmethod vaic'hvoll be used inthe future. Recommendations forAdditioniTWesting: BOD, COD, fish,algae,and Micmtox. 6.Filmor (FM-3820 or FC-95) Degradabift BODS. BODIO BOD20 .COD BOD/COD Toxicily: nil(PED) nil(PED) nil(PED) nil(PED) 0 -18- WaterFlea(Daphnimaagna) 48-hrStatiEcC50 48-hr StaticEC50 49 mg/L (EnviroSystems1991) 50 mg/L (PED) ActivatedSludge Inhib7itoCroync. >4,000mg/L (PED) FatheadMinnow (Pimaphatespromelas) 96-hrLC50 38 mg/L (PED) BluegilSlunfish(Lepomismacrochirus) .96-hLrC50 68 mg/L (PED) Rainbow Trout(Salmogairdnen) 96-hrLC50 11 mg/L (PED) -Env*ronmentalAssessment: The flimerwillnotcontributteothe BOD/COD ratios,o toxicitiystheissuewiththis component.Thismateriadloes show slightoxicityofishand Daphnia There isgood agreement between previousand presentDaphnia toxicitdyata which givesus confidence inthe most recentdata from EnviroSystems. We need to have a completesetofeffectsdata fora sample representativoefa recent manufacturinglotwhich has been produced by the manufactudng method which willbe used inthefuture. Recommendations forAdditionaTlesting: Algae and Microtox. 7. Na-octylsulfate(RM 32067 or RM 5504015) 142-31-4 Vendors:AlcolacIncorpomted'6(Sodium OctylSulfateSolution)S;tepan Company 17 (POLYSTEP B-29). Degradabift THOD 1.88g/g (calculate-d) 15 Wim Vanneste'sPROFS notedated 03/12/1990givesthisRM#. 16 Alcolac:301-355-2600.No data available. 17 Called708-446-7500 ext.2283: they have no data. -19- BOD SWisheO" reportsthat"lineaprrimaryalkylsulfateshave longbeen recognizedas extremely-mpidinprimarybiodegrackwon,oftendisappearing inlessthan a day" and citesapproximately100 referenceswhich show degradationratesof80 to 100% withina few hoursordays. Toxic7ity9s ,AustralianBamacle (Eiminius modestus) 30-min StaticImmobilizationEC50 3,970 mg/L (AQUIRE 2; 1976) Water Flea(Daphniamagna) 48-hrStaticEC50 517 mg/L (Env!mSystems 1991) EnvironmentalAssessment: --Afthoughno BOD or COD data are ava@ilablteh,ismaterialwillmost likelhyave a high BOD/COD ratioB.OD and COD data shouldbe obtainedso thatwe have numericalvalues which have been obtainedintestscomparable tothose used forthe remaining components. There issurprisingllyittlieteratudraeta availabloen the aquatictoxicitoyfthissurfactant, however forthe Daphnia data itshows considerablylesstoxicittyhan the C 2 surfactant. This surfactanitspresentat3% so itmay be a:majorfactortotoxicitoyfthe product.Fish, algae,and Microtoxtoxicitdyata areessentiatlocomplete the assessment ofthis ingredient. Recommendations forAdditionaTlesting: BOD, COD, fish,algae,and Micmtox. S.Di-water(RD 199) Toxicily: Water Flea (Daphnia magna) 48-hrStaticEC50 >1,000 mg/L (EnviroSystems1991) EnvironmentalAssessment: While itmay seem foolishtotestthe.deionizedwaterfortoxicittyo,xiceffectshave been tracedto contaminateddeionizedwatersuppliesinthe past.The Daphnia resultindicates thatthe supplywas notcontaminatedand no furthetrestingofthe delonizedwatersample iswarranted. Recommendations forAdditionaTlesting: None. 18 Swisher,R. D. SurfactantBiodegradation2,nd ed, Marcel Dekker:New York,1987,p 466, 807-811. 19 There was 1 testgiveninthe EPA AQUIRE database. -20- 9.FC-203 DogmdabilftZ BOD5 BOD20 COD BODr,/COD Toxicilye Micmtox EC,o EC50 ActivatedSludge lnhiuiton Algae (Chlorellpayrenoidosa . andPhormicriunmundatum) Algae(Scenedesmussubspicatus) 72-hSrtatiPcopulG.rowthlnhiEbC.lo 72-hr StaticPopul.Growth Inhib.EC50 Water Flea (Daphniamagna) 24-hrStaticEC,, 24-hrStaticEC50 48-hrStaticEC50 48-hrStaticEC50 560 g/L(PED) 717 g/L(German Army Report") 1,060g/L(PED) 1,070g& (PED) 1,373 g& (German Army Report) 0.52 (PED and German Army Report) 632 mg/L (German Army Report) 2,500 mg/L (German Army Report) none at1,000mg/L (PED) no effectat:91,000mg/L (PED 21) 16.9mg/L (German Army Report) 160.-mg/L(German Army Report) 31.6 (German Armypoport) 430 mg/L (German Army*Rbport) 229 mg/L (EnviroSystems 1991)22 1,600mg/L (PED23) 20-"3% applicatiosnolutionsw"ere used toobtain'theBOD an d COD data inthe German Army Report.The German Army valueswere 41,200 mg/L and 21,500 mg/L forthe COD and BOD5, respectivelyT.he German Army valueswere multipliebdy 33.3 to getthevalues inthisreport. 21 Testingdone by IndustriaBlio-TestLaboratory,Jan. 1974. 22 EnviroSystems isrepeatingthistestbecause therewas not enough variatioinnthe FC-203 concentrationsthey used todevelop a good standardcurve. 23 Testingdone by Bionomics Laboratory,Dec. 1973. -21- EasternOyster Embryo-larvae (Crassostrevairginica) 48-hrStatiEcC5. 47 mg/L (PED24) Grass Shrimp (Palaamonetespugio) 96-hrStaticLC5. 510 mg/L (PED25) Fatheadminnow (Pimephalespromelas) 96-hrStatiLcC50 750 mg/L (PED) Zebr.afi(sBhrachydaniroetio) 48-hrStaticLCO 48-hrStaticLC50 1,053mg/L (German Army Report) 1,634 mg/L (German Army Report) Scud (Gammarus fasciatus) 48-hrStatiLcC5. 1,100 mg/L (PED26) Rainbow trout(Oncorhyncusmykiss27) 96-hrStaticLC50 1,300 mg/L (PED) Rainbow trout(Oncorhyncusmykss) 96-hrContinuousFlow LC5. 400 rh@ (PED28) Common mummichog (Fundulusheteroditus) 96-hrStaticLC,, 2,500 mg/L (PED29) Grass Shrimp (Palaemonetespugio) 96-hr LC50 510 mg/L (PED) EnvironmentalAssessment: 1.The fishtoxicitdyata from the German Army Report agree@ wellwithdata from previous 3M studiess,o no fishtestingisneeded. 2.The Microtoxtestsfrom the German Army Report seem reasonable,so thist@itShould notbe repeated. 3.The 24-hrEC50 Daphnia toxicitfyrom the most recentEnviroSystems work and the 48-hr EC50 from theGerman Army Report are infairlgyood agreement. However, we weren't satisfiewdiththeconcentrationussed inthe EnviroSystems work-and they are repeating 24 Testingdone by Bionomics Laboratory,July 1980. 25 Testingdone by Bionomics Laboratory,Aug. 1979. 26 Testingdone by BionomicsLaboratory,Dec. 1973. 27 FormerlySalmo gakdned. 28 No mortalitoyrabnormal effectsatthisconcentration. 29 Testingdone by BionomicsLaboratory,Aug. 1979. -22- thiswork.The largedifferencebetween German Army valueand the 1973 PED value may be due toformulatiocnhanges orchanges inthe toxicittyestprotocolbutthiscannot be verified. 4.Ai withthefoamer and filmerw,e need tobe carefulthatthe sample submfttedfor testingisofknown compositionand from a known manufacturinglot. 5.Sincetoxicittyo algaewas ofconcern intheGerman probablythe most important. Recommendations forAdditionaTlesting: BOD, COD, and algae. Army Report,the algaedata is 10.VW1 60390 (FC-2D3 without TolyltriazolReM 27513) Toxidily: Water Flea (Daphnia magna) 48-hrStaticEC50 165 mg/L (EnviroSystems1991)30 EnvironmentalAssessment: 1.Sincethe onlydifferencbeetween thismaterialand FO-203 istolyftdazoltee,stingthis materialalongwithFC,203 seems likea good way to.determinetheeffectoftolyftdazole. Forthe recentDaphnia data,thereislittdliefferencbeetween FC-203 and Vwl 60390 and theeffectoftolyltdazolceannot be seen. Infact,thetoxicitpyfFC-203 was slightllyess thanthe toxicitoyf VW1 60390 inthe recentEnviroSystemswork. S@inceEnviroSystems is repeatingthe Daphnia forthese two samples, we Willhave to waftuntiElnviroSystems has completed thesetestsbeforemaking an evaluation. 2.A Microtoxand fishtoxicittyestshouldbe done on thismaterialand the results compared tothe FC-203 resulttsosee ifthereisa measurable effectcaused by tolyltdazolienthe product. Recommendations forAdditionaTlesting:" BOD, algae,fish,and Microtok.*' 30 Envir6Systemsisrepeatingthistestbecause therewas notenough variatioinnthe concentrationtshey used todevelopa good standardcurve. -23- 1.FC-203CE Dogmdabeft BOD,s 91 9/L (PED) BODIO 400 9/L(PED) BOD2o 680 9/L(PED) COD 762 9/L(PED) BODMICOD ratio 0.89(PED) TotalOrganicCarbon (TOC) 220 g/L (PED) OECD Method 301 E 88-90% biodegradability in14 days (PED) Toxicity* Algae (Selenastrumcapticomutum) 95-hr IC50 >.1,000mg/L (PED) OECD ActivatedSludge Respiration InhiUitioTnest 209 none at1,000 mg/L Water Flea (Daphnia magna) 48-hrStaticLC, >1,000 mg/L (PED) Micmtox (Photobactetium Expos'ure: 5 min. 15 min. 30 min phosphoreum) EC50 370 mg/L -260rdg/L 230 mg/L Killifi(sFhundulusheteroclitus) 96-hrContinuousFlow LCso 1,400mg/L (PED) FatheadMinnow (Pimephafespromelas) 96-hrContinuousFlow LC50 96-hrStaticLC50 >2,000 mg/L (PED) >1,000 mg/L (PED) EnvironmentalAssessment* The environmentaleffectsdata forFC-203CE isfaidycomplete. The PED data shows lowertoxic7dtyoDaphnia than isshown'by FC-203 intheGerman .'Army Reportand from the recenttestsby EnviroSystems.Daphnia testingshouldbe done againforassurance inour PED. Recommendations for-AdditionTaelsting: Algae and Daphnia. -24- 12.FC-206CE Dogmdabib; BODs 46 9/L(PED) .BODIO- 260 9/L(PED) BOD2o 470 9/L(PED) COD 402 g/L(PED) BODMICOD ratio 1.2(PED) TotalOrganicCarbon (TOC) 130 g/L(PED) OECD Method 301E Toxicilye 89-92% biodegmdab7ll4 in14 days (PED) AJgae (Selenastrumcapdoomutum) 95-hr IC5. >.1,000m@t (PED) OECD ActivatedSludge Respiration InhibitiToenst 209 none at1,000 mg/L Water Flea (Daphnia magna) 48-hr StaticLC50 >1,000 mg/L (PED) Microtox(Photobactatiumphosphoreum) Exposure: 5 min. EC50 650 mg/L 15 min. 30 min -4500i@@ 4200 mgAL Killifi(sFhundulus hateroctitus) 96-hrContinuous Flow LCo >2,000 mg/L (PED) FatheadMinnow (Pimephaiespromelas) 96-hrContinuousFlow LC. 96-hrStaticLC50 >2.000 mg/L (fED) >1,000 mg/1-(PED) EnvironmentalAssessment: The environmentaleffectsdataforFC-203CE isfairlcyomplete. Aga7int,he.PED datashows low toxicityo Daphnia which sho*uldbe verified. RecOmme ndationsforAdditionaTlesting: Algae and Daphnia. -25- 13. FC-2D3CF EnvironmentalAssessment: No environmentaleffectsdataforthisproductare availableand a completesetof environmentaleffectsdata should be obtained. Recommendations forAdditionaTlesting: BOD; COD, algae,DaphniA fish,and Microtox. 14.FC-206CF Env! nr@entalAssessment: No environmentaleffectsdata forthisproductare availableand a complete setof -environmentaelffectsdata should be obtained. Recommendations forAdditionaTlesting: BOD, COD, algae,Daphn!A fish,and Microtox. 15.L-4640 (New Foamer) Testingiscurrentluynderway fora European PMN by NOTOX* Laboratories: 1.Daphnia toxicitsytudy(C2 EEC testmethod). 2.Fishtoxicit(yCl method) usingZebra fish(Brachydaniorerio). 3.-ModifiedSturm test(C5)forreadybiodegradability. Alltestingforthe European PMN isbeingdone by GLP. The followinegnvironmentaleffectsdataare fmm several3M technicalreportswhich are compiledinthe report"EnvironmentalData and InformationinSupportofthe PMN for Certa@in-ChemicaSlubstances Designated as L-4640",June Degradabift BOD5 BODIO BOD20 COD TheoreticaOlxygen Demand Toxicily: Acute Effecton MicrobialRespiration Water Flea(Daphnia magna) 48-hrStaticLC50 Fathead Minnow (Pimephalespromelas) 96-hrStaticLC50 <0.038 g/g <0.038 g/g <0.038 g/g 0.41 g/g 0.75 g/g 8% reductioninrateofoxygen depletionat1,000 mg/L 100 mg/L and 111 mg/L 158 mg/L and 166 mg/L -26- EnvironmentalAssessment& Because ofthe importanceofthismaterialtofutureproducts,a fullsetofenvironmental effectsdata isneeded.The environmentaleffectsdata not includedinthe PMN work or in the--Pre*viousstudiesshouldbe obtwned: algae and Microtox. Rommmandations forAdditionaTlesting: Algag and Micmtox. 16.Witcolate7093 (RM 45666) CAS # 73665-23-2 -WrtcoCorporation(713-433-7281)has no ecotoxinformationavailableW.doblate 7093 is 55% water,45% C6-Cl 0 ethoxylatedsulfateT.hey recommended SvAsherfor biodegradationinformation. No informationon EPA AQUIRE database nor inVerschuere-n. EnvironmentalAssessment: No effectsdata isavailableT.estingdepends on the likelihootdhatthismaterialwillbe used infutureproducts.' R!goommendations forAdditionaTlesting: CODD- BOD, algae,fish,Daphnia,and Microtox. 17.Triethanolamine CAS 102-71-6 DegradabilWI THOD BOD5 BODIO BOD,5 BOD20 2.04 g/g nil 0.02 g/gwithstandardseed 0.17 g/g adapted sewage 0.03 g/g 0.02 g/g' nilwithstd.dilutedsewage 0.05 g/g 0.13 g/g 31 Alldegradabilitdyata are fromVerschueren p 1150-1. -27- COD BODWCOD BODMICOD 1.50 g/g nilto 0.11 0.09 Bacteria(Pseudomonas putida) Popul.Growth Inhib. Algae (Microcystfaseniginosa) Popul.Growth lnhib. Green Algae(Scenedesmus quaddcalda) 7-dayStaticPopul.Growth lnhib. 8-dayStaticPopul.Growth lnhib. 8-day StaticPopul.Growth Inhib. Blue-greenAlgae (Anacystisaeniginosa) 8-dayStaticPopul.Growth Inhib. Protozoa(Entosiphosnulcatum) 72-hrStatiPcopulationGrowth Protozoa(Chilomonasparamecium) 48-hrRenewal Popul.Growth lnhib. Protozoa(Uronema parduczi) Bacteria(Pseudomonas) LCO Algae (Sconedesmus) LCO Algae (Colpoda) LCO Water Flea(Daphnia) LCO 24-hr StaticLC50 BrineShrimp (Attemiasatiria) 24-hrStaticLC5. >10,000 mg/L (Verschuerenp 1151) 47 mg/L (Verschuerenp 1151) .1.8mg/L (AQUIRE 2;1980) l..8mg/L (AQUIRE 2;1078) 715 mg/L (AQUIRE 2;1978)33 19 mg/L.and 47 mg/L (AQUIRE 2;1978) 56 mg/L (AQUIRE 2;1980) 1,768mg/L (AQUIRE 4;1980) >10,000 mg/L (Verschuerenp 1151) 10,000'mg/L (Verschuerenp 1151) 100 mg/L'(Verschuerenp 1-161) 160 mg/L (Verschuerenp 1151) 2,500 mg/L (Verschuerenp 1151) 1,390mg/L (AQUIRE 2;1977) 5,600 mg/L (AQUIRE 2;1974) 32 The toxicitdyata are a partialjisntgi ofthe 16 testson 10 speciesgiven intheAQU IRE database. 33 Thisvalueisfrom a differenrteferencethan the previousone. -28- Goldfis(hCarissiauusratus) 24-hrStaticLC,, 5,000mg/L (AQUIRE 2;1979) 24-hrLC50(85% TEA) 75,000 mg/L atpH 7 3,500 mg/L atpH 10.3 (Verschuerenp 1151) EnvironmentalAssessment: This materialshows insignificatnotxicittyo Daphnia and fish. However, ithas a low BOD/COD ratioand willcontributteoa low ratiointhe product.In additioni,tshows considerabletoxic7ittoyalgae.For these reasons,itwould be bestnotto use thismaterialinproductsunlessno aftemativecan be found oritsconcentrationinthe productwillbe extremelylow. The literatudraeta isessentiallcyomplete forthismaterialA.ny testingon thismaterialmust be reportedto US EPA undersectionTSCA 8(d). Recommendations forAdditionaTlesting: None. 18. Urea CAS 57-13-6 Degmdabifibt@4 Degrades abiotically: H2NCONH2+ H20 C02+ NH3 De@mdation rateat20'C Degradationrateat2*C Concentrationindomesticsewage 16.9 mg/(-Jhr 3.3 mg/L/hr 2 to6 mg/L Bacteria(Pseudomonas putida) Popul.Growth lnhib.. Green Algae (Scenedesmus quadricauda) 7-day StaticPopul.Growth lnhib. 8-day StaticPopul.Growth lnhib. >10,000 mg/L (Verschuerenp 1178) 10,000 mg/L (AQUIRE 2; 1980) 10,000 mg/L (AQU IRE 2; 1978) 34 Alldegradabilitdyata arefrom Verschueren p 1178-9. 35 The toxicitdyata are a partiallistinogf32 testson 17 speciesfromthe EPA AQUIRE database. -29- Blue-greAelngae(Anacystaiesruginosa) 8-dayStatiPcopulG.rowthlnhib. 47 mg/L (AQUIRE 2;1978) Protozoa(Entosiphonsulcatqm) 72-hrStaticPopul.Growth Inhib. 29 mg/L (AQU IRE 2;1980) Protozoa(Chilomonasparamedum) 48-hrPopul.Growth lnhib. 2,683 mg/L (AQUIRE 4; 1980) Water Flea(Daphnia) .24-hSrtaticLC50 10,000 mg/L (AQUIRE 2; 1977) Creek Chub (Semotilusatromaculatus) 24-hrLCO 16,000 mg/L (AQUIRE 2; 1952) 24-hr LCIOO 30,000 mgli-(AQUIRE 2; 1952) EnvironmentalAssessment: . Uteraturedata indicatetshatthismaterialshows insignificatnotxicittyo'DaphniAfish,and green algae.Furthermore,itdegrades readilyand R.most likelwyould notbe a problem in the product. However, itdoes show toxicittyoblue-greenalgae,and the low toxicittyogreen algae should be verifiebdy. our own testsifitistobe used inthe productathighconcentrations. Recommendations forAdditionaTlesting: None. 19.TritonX-305 CAS # 9002-93-1 (PRISM shows Tdton X-305 to be 70% thismaterialwiththe ,-Compositioonfthe remaining30% notgiven). Synonyms: Poly(oxy-1,2-ethanediayllp)h,a-[4-(1,1,3,3-tetramethylbutyl)phenyl]omega-hydroxy-polyethylengelycolp-1,1,3,3-tetramethylbutylphetnhyelr Octoxinol P-tert-octylphenoxypolyethoxyethanol Vendore Roh,m & Haas Company-''6 36 Independence MailWest, PhiladelphiaP,A 19105. Phone: 215-592-3000. -30- Toxidil3y7, Bluegil(lLepomis macrochirus) 24-hrStaticLC50 24-hr StaticLC50 24-hr StaticLC50 24-hrFlow-throughLC.0 96-hrStaticLC5. 96-hrStaticLC5. 96-hrStaticLC5. 96-hrFlow-throughLC50 6-day Flow-throughLCao 3.5 m,g/L(AQUIRE 1;1975) 16.2mg/L (AQUIRE 1;1975) 1,080Mg/L (AQUIRE 1;1975) 10.0mg/L (AQUIRE 2; 1975) 2.8mg/L (AQUIRE 1;1975) 12.Omg/L (AQUIRE 1;1975) 531 mg/L (AQUIRE 1;1975) 1O.Omg/L (AQUIRE 2; 1975) 9.6mg/L (AQUIRE 2;1975) The reasonforthehighvaluesforthe24-hrStaticLb5o (1,080mgAL)and forthe96-hr StaticLCso(531 mg/L )are notknown. The vendor forTritonX-305 (Rohm and Haas) has no data on the.aquatitcoxicitoyr biodegradabiliotfyTritonX-305. However, they are.sendinga!data sheeton the.aquatic toxicitoyfTritonX-100.TritonX-100 has a lower molecularWeightthan X-305 and they believethatthetoxicitoyfX-305 islessthan X-:0l0 because X-305 islesssurfaceactive. TritonX-305 isnotbelievedtodegrade biologicallRyo.hm and Haas has recentlysoldtheir Tritonproductlineto Union Carbide (1-800-752-1827)and YAIIbe transferrintghe business in4henear futureT.he CAS number givenby Rohm and Haas forthe seriesis9036-19-5. The followindgata isfrom AQUIRE on thisCAS number. CAS 9036-19-5 Rainbow Trout(Salmogairdnen) 48-hrStaticLC5. 96-hr StaticLC50 7.2mg/L (AQUIRE 2;1978) 7.2mg/L (AQUIRE 2;1978) MarinePolychaeteWorm (Scolelepifsuliginosa) 48-hrStaticLC50 96-hr StaticLC50 17.0 mg/L (avg.of7 points) 13.7 mgAL (avg.of8 points) (AQUIRE 2;1978) 37 The toxicitdyata are a partiallistinogf13 testson 5 speciesfrom the EPA AQUIRE database. 38 The toxicitdyata are a partiallistinogf36 testson 5 speciesfrom the EPA AQUIRE database. -31- MarinePolychaetWeorm (Cgotelicapitata) 48-hrStatiLcCOO 96-hr StaticLC50 8.2mgAL (avg.of6 points) 6.0mg/L (avg.of8 points) (AQUIRE 2;1978) AquaticSowbug (idotebaafthica) 48-hrRenewal LCso 96-hrRenewal LC50 Eh ronmentalAssessment* 10.0 mgAL (AQUIRE 3 ;1972) 5.0 mg/L (AQUIRE 3;'1972) Both CAS numbers have a limiteadmount ofto)Cicidtayta availablwehich shows moderate toxicityS.inceTritonX-305 isa majorcomponent ofthe CE products,we willneed a --completesetofdata on,thismaterialinorderinordertocorrelattehe effects.otfheCE productstotheirformulation. Recommendation s forAdditionaTlesting: BOD, COD, algae,DaphniA fish,and Micmtox. -32-