Document 377rdyJLy6ggMNpLeJgdQnw1J
CER
en
sees IR 226-0006
Free RECEVED
Extent and Route of Excretionang
acDteirstsriSbuitniaoin oInftrTaovtaelnoCuasrbon-]
rFEIE,|
uN 50 12
= aM
Deceaber 2, 199%
1oxicOL8
Conducted at:
During: Conducted by: Report. by:
S5R8ui.kbespridaLiuaa,broyMrioanttonreoisneost,a
Tne. 35101
2
une to Gotaber, 197
5. 3. Gibson and 3. b. sonnsen
enioDr boioda)pdansenthPsracmarYotiba/i2o3e0
Reviewed by:
R{aDmO aige kDree gEfolcaboSiaiorns Y lg0
000037
.
RV
Summary
2
By 89 days after a single iv dose of FC-95-'%C (mean dose, 4.2 mg/kg) six
male rats excreted a mean of 30.23 of the total carbon-1k via urine. Mean
oc2uf0.m6ut;loatatplilvaescmaarf,becoan2l.-21;hexcekrxiepdtrneieyos,nsedw1a.s1a3s 1y2Tg.u6n3gF.,G-91A5.t-1$;149Cspsldeqaeyunsi,,val0me-e5na;nts/atgnidswseubreoen:ecomnacirevrneetrnr.,ation
0m.u5s."cle,Lowfeart,coanndceneyter.atioNnosra(d<i0o.a5c)tiwveirtey m(eas0u.0r5e)d wiansaddreetneacltse,d sikninb,raitne.stes,
Trheespeccatribvoenl-y1.4 in liver and plasma represents 25 and 3 percent of the dose,
Introduction
#C5--5500,,"K*
F * Denotes position of carbon-14
FC-95 is the potassium salt of a perfluorinated sulfonic acid. A series
odtfefhresoiengxrnppaeltroaeisfnmoaeft,nhteesslaenidmhieansxeapxetcbrireioeemntneinooptnflsa.dnFanCte-aTd9h5iwtseofrreFoimCnn-vete0chs5eet-1sihgbgaoadrtyyeI.npttoorsaFsvCfei-onb9ro5lmuesaabmesebxoaaprnsepsirtsiiomoffenon,tfi.noocffr(fhemieaiscsniiaentngiton Experinent 3) which was designed to provide data on thor route and extent
of total carbon-14 excretion was paired with an FC-95-14C oraldosing
oeaptnlxhdapesernreailemixenepniletinrmaiiwtnnhieaiotncnithosfnwr,aoosnnanpdtdleheasseimpxgaocn.sresedit(bi2tlo)oenprbdIaointvoaitadrdwedaiinltsdlifaootnarb,meaotnutishoeetndsoteaotlfFoC-cFi9Can5-rt8be0aor7bnps-ro1o(r4s3p)atbainstogoonr,Fgpu0ti9id5oe.n Methods
Radiolabeled Fc-95-14c
The carbon-14 label is at the position a to the sulfur2fgm (see above structure). The specific activity of this lot of fos 'C (Riker Isotope
sdIeuntiveteranmbtiloneraytfiNoorunm,bmeertanad4b4o2lr)iasdmiios csht0eu.md4ii5ce9asl;uCipd/uemrtgia.tiylsTdheoetfertFmhCie-n9a5st-yi'no9tnCheswhiaassv,e fbsoepuenendcifrtioecpobreaccetdi:vit(y1) Animals
Dosing Six male Charles River? CD rats, eight weeks old, were conditioned to
individual metal metabolism cages for 24 hours prior to dosing. The body
waceciegshsts toranPguerdinfaroGmro2u6n2d toCho3w03angd,
mean 283 g.
water before
The
and
rats were allowed
after dosing.
free
2 Charles River Sreeding Laboratories, Wilmington, Kass. . Esiancghlerativwadsosweel(gvhiead,taialnesvetihne)tizoefdFwCi-t3h5-1d4lCe.chy)Theethdeors,e awnads 2t.h0enmigiovefnaa
0.9% NaCl solution containing 1.21 mg FC-35-14/2.0 ml. The average
dose was 4.2 mg/kg. The dose was delivered with a 3.0 ccdisposable plastic syringe (Monoject) fitted with a 26 gauge 1/2" needle. The
2 Purina Lab Chow, Ralston Purina Company, St. Louis, Missouri.
Sherwood Medical Industries, Inc., Deland, Florida 32720. 000038
[on .
3
cdseohnsatikrniignfgusgofilonrugt.oinoen-Thhweaalsfsupprheoerupnraarteaatdntmboydwaeasrdadtirenegmos%vpeede2d0a0nidmngausofemdecFhfCao-nr3i5cd-aol'sCinsghtaokseo0rl.,u9t%iaonNnda.Cl, Tchoeuntcianrgbon(-s1e4e Acpopnetenndtixof1).the dosing solution was determined by direct
Sample Collection
owUfirtihntehediaenstdihxyflreacteestshewfreo;rre8b9lcooolddalyeswc.atsedAdtraatw8n9indftareyorsmvalptsohset(dsedesoesceeT,anbdlitenhseg
r| atasndwe2)refaornesetachhetized aorta and immediately.
ctleirnvaetnrrs,iffeurbgrraaetidin,otno.teastThehesep,arraiadntsriezwneaedlrset,ubseak.cidrniefPyilsca,esdmalbuwynagsse,xsparannegdpuaireneyaedstipowrnoo;rmeptaclnoydllbseypclteeend,
2rseawrholleegsorbgyanssp.littBionnge tared combustion padd.
mtaherrobwonewasandobtcaoilnleedctifnrgonthtehemafroruorwmaonjorpiebcoensesofofa the Samples of skin, thigh muscles, subcutaneous and
abdominal fat, and the total remaining carcass were collected.
Sample Analysis for Carbon-14
agFneednceiszailniagnqduwoatmsianjdgoornaeorsgaiamnnpslWearwieonfrge tpbherleepnhadoremerodsgenbfayotraedcdaiirnnbtgoon-cn1io4nmebuasnptaairlotynssiscofonbweya2t.heormogH(eowmn/owi).zing
cctooonmebounesatnidpoanratddcioonfngesbi1o.i0lnoggtircOiafpllitmchaaetteehroiomaonlga.enattToehp.e-lohCaoadmrioenggenwaabstaelsatnawkceeenrebytwoefimagirhixendgthetihneto
mheoamsougreendateinbtoetwceoemnbusstaimopnlincgosn.es Ubyriwneei,ghrte.d samples of bone marrow, skin and fat were
blWohoodleceelyless,, also weighed
aanddrenpallass,msawndere In combustion
croenmeosv.al samples
Cianreordwears ttoakeanvoitdo weighed directly,
wleoisgshofthweesieghstampblyesdrypirnogm.ptlyHom(o1g-e2namtiensu,test)isasufeter and weighed samples of piasma, red blood cells,
acbnladarnbkounr-ih1no4emgwfeernroaentecbsioomlb(oufgseitcceeasdl, wis1taihmvperla,esPamuwcsakcsalrgdd,etMoeadrnedmlinsep3dl0e6ebnyO)xcidoainmzdbeursb,tlianngkRecsuorivatcearbyleo.f
pmcilodaldsllmeeac,tisopaninsdkewdeenrdweitohfsamdtpihlleeudetxiopbneesrfiomroeefntfFarCle-e9z5si-an1mg4pClaenddosestwienrg(esseeocolAuupnttpieeodnnddiaitxret2c)ht.elyb;eUgriinnening,
sttcoriinprtleiiflcrlaiatgteeiroa1nt.o0rvimlatlesamlpieaqnrduaottu1sr5emolfinAeaqtcuhehasdosaalrmkpwlaebsefwoeardrdeeedcp.oiupnettAiltnlegd.sdaimrAplellcestlrywaedriIeonmtecotoroilced
aSnpaelcytsreosmetweerr.e done using a Packard Model 3385 Tri-Carb Liquid Scintillation
msCtaoatunindctairnEdgx,teerfinfnaitlceirneSantlcayndsatfraodnridzaearatdcihownas)samaprdladeteidwoasmteotdhesotedel.remcitneTedod scaabmylpilbuersseatoeffrotmhtehetehexAtEeSgrrno(auAlputooctfohleslseeacmtpseladempslone(stphwurenerecehwitrtaephceoulanontwdedAEpSraolocrneagstsieowdsithabnydathtseheraelCeeOdCwis1tt7ha0n0dhaiCrgodhm.purtaeDtraitoasS)y,wsetraeenmd in wctaohsempua3tcMecroCmepnptlrroiagslrhaemdR.ewsietahrchtheDatBaiolPorgoicceaslsinAgutoLmaabtoiractoErxyt.ernaDlataStarneddaucrtdiioznatitoondpm
2 Packard Instrument Company, Inc., 2200 Warrenville Road, Downers Grove, IL.
000033
oo.
a
Results and Discussion
aTnhde irnesuFlitgsureosf t1 heandfec2.al as percent of dose. The
Tdaanabdtlaeusarrien1 aaernxydpraen2saslMeyidssetsastahreceumtusolthaaoltwnivceairnbpoeTrnac-be1lnhetseox1pfraednosdssee2d
excreted versus time in Figures 1 and 2. As Illustrated in Figures | and
2t,otaelxcreextciroentioonf t(outrailneca+rbfoenc-es1)4 iiss osnlloyw. 43 Bpyer8c9endtayosfafttheerddoossei.ng,Radioactive
content in feces excreted is thus
was the
too sum
low to measure after 64 days; total carbon-14 of fecal excretion (0 to 64 days) andurinary
etxoctarleticoanrbo(n0-1tho 8in9 udaryisn)e. (3T0h.e23)meatno trhaattio ionf fcecuemsula(t1i2v.e63)exwcarseti2o.n o+f0.2.
TihininsgulreiinseivintdoocsotenhattorfasitFnC-tf8eoc0e7ts-h,1e1(Cr<.a0t,i(1o3)) oaftTci1u2sm4uldiaanytdsiivceaptoeesstxcrdteohtasteionFrCeop-foBr0tt7eo-td1a,%laftcieasrrnbooatn-14
FlCi-k9e5l-y1%extdeonsseivwealsy nboitoterxacnrseftoerdmedby teoitFhCe-r95-f1e4cCe.s orSinucreine50a-t6089perdcaeysnt poofst the
dtohsoes,e tsieslseucetsed intiwshsiucehs wteortael ancaalrybzoend-1hformigcahrtbobne-1krhetacionnetde.nt to identify
The results of the tissue analyses are shown, jn Table 3. Kean tissue
ctfaoirlsblsoouwness-:14succlhoivnecarse,nmtur2sa0ct.l6ie;o,nsplskaaisbnmo,av,ebo2on.n2ee; mugaKrirdFonCwe-,y9,5-an11d7.1;sepqalnuedienvallhueanggn,tsc/o1gn1c+weenrterOatthaiesrons
orfansguinbgcutfarnoemou0s.2 fatto 0(.06.2 uugg//g.g) aTnhdereabdwoamsinaaldifffatere(n<ce0.0iBnugc/aer)b.on-V1e4ryco1n1tteenrte
-otcfoatratblootnac-la14rbcwoaanrs-b1o4nf-ow1ua4nsdrefitnoeunnbdtraiioinnn. wwhheoTnlheerleeiyveeirs,(0ab.o1sn6teruimgka/igrn)rgoawdn,idfafnendroensdcpeelteeecintnabatlfheteerptaottiatvlern
dftoohrsesienFtgC-iw8si0st7uh-e1s4FcC-ad3fo5ts-ee1rd4CdorasitissngconwmoirptmaharleidFzCe-td8o07tth-oe',7plCia.vtetr(e3r)fngntToehfnetrmeeatraeenn:ticoanrlbiovfenor-u,n1d4 1c.f0oo;nrtent
srspapltlieeoeesnn,/lair8ve.e9;rfiavrneadrt,iobo1nf.e0o;lmlaosrwprilonewge,n,FC2.-049..052-;14FCaonrdisFbCo8-n.9e95-1man4adrCrobdwoo,nseed0.m0ar2ra.trso,w/`S1tiihnevceemretahne
oirs 2r.ahp,idlythibsiotdrataansfsourpmpeodrtstothFeC-9c5o.nclusion that FC-807 is not extensively
sTheelecttoetadl whcoalrebono-r1g4anscoanntdentpslasemxapreasndsedredasblpoeordcenctellosf adroese lfiosrted
aigneTaofblethe&. dosOenlyat found for kidney,
l8i9verdayasndpopsltasmdaosec.ontaTihne alowsublsetvaenltsiaolf lung, testes, and spleen, are (in view
preardcieonatc-tivity of the
drueelatiinveplayrthitgohthcearblbooo1dnstciolnltenctonotfainpeldasmisn atnhdeseredorbglaonsodwhceenllsh)onogen-
ized and analyzed.
000040
.
-5-
References
(1) J(oRhenpsorotn),Jo,NovBeemhbrerFE:2, S1y57n9t.hesis and Characterization of Fc-95-14c
(2) JDoohsenso(nReJpoo:rt) AbOscotorbpetrion26,of 19F7C9-.95-14C in Rats After a Single Oral
(3)
Johnson Jo: Extent o(fReTpootratl), CSaerpbtoenm-b1e4r
and Route of i2n8,Rat1s979A.fter
Excretion
a Single
and Tissue Distribution
IV Dose of FC-807-14C
(4) AM1al9rt7y1m,laann5dP.,L5,.FeDdietrtamteironDS:of ABmleoroidcaanndSoOctihsetrTeBsod FoFrluEixdpse.rimeBnettahlesd8ai,ology,
000041
List of Tables and Figures
Table 1: Table 2: Table 3: Table 4: Figure 1: Figure 2: ATpapbelnedix1,: ATpapbelnedix2,: ATpapbelnedix3,:
EIxnctrreatveinoonusofDoTsoetalofCaFrCb-o9n5--114Cin(MUerainneDosien,Ra4.t2s amfat/ekrg).a Single NB 52584 p. 17.
Excretion of Total Carbon-14 in Feces in Rats NIBntr5a2v58e4noup.s D14o.se of FC-95-14C (Mean Dose, 4.2
after a
mg/kg).
Single
2TissisnugeleDisItntrriabvuetnioounsoDfosTeotoafl NB 52584 p. 20.
CFaCr-b9o5n--1144C
i(neaRnatsDosaet, 849.2Damyse/akfgt.)er
NPIBenrtcr5e2a5nv8te4noopuf.sDDo2o1s.seePorfesFeCn-t95i-n1T4iCss(uesasnaDtos8e9,Da4y.s2 mafgt/ekrg).a Single
ICVumuDlosaetiovfe EFxCc-r9e5t-i1o4nC NB 52584 p. la.
o(feaTnotaDloseC,arb4o.2n-m1g4/kign) UMreianne
aoffterRaateS.ingle
ICuVmuDloasetiovfe NB 52584 p.
EFxCc-r9e5t-i1o4nC 14,
o(fMeTaontaDloseC,arb4o.2n-n1g4/kign) y
FeMceeasn
oafft6erRaatsS.ingle
NDBete5r13m1i2napt.ion35.of Carbon-14 Content of Dosing Solution.
SRaemcpolveesrySpofikeTdotwailtCharFbCo-n9-51-414cf.rom Blank Fecal Homogenate NB 52584 p. 16.
OCxoimdpaatriiosnonVeofrsCuasrbDoinr-e1c4t NB 52584 p. 9.
ACnoaulnytsiinsg
oifn
UArquianseolSa.mples
by
000042
.: =
<= fable 1 iEnxcRraettsioanftoefr aToStianlglCaerbIonnt-r1a4venionusUriDnoese of FC-95-14C (Mean Dose, 4.2 mg/kg)
GPeorllloods(tdiaoyns]2 3 1 3iy p2 5 e agg Ee
00-.05.15 1-2
01..70002
oles 0.50
1.56
11
1.03 0.72 1.47
0.77 0.60 0.78
1.03 0.77 1.08
0.86 01..9247
2-3 43--45
1a 11.l1069
1.05 00..8807
114 10..0861
0.68 00..6680
0.91 00..9830
1n 00..9805
65--76 7-8
01..8067
00..7721
00..7657
00.l4s92
00..7764
00..7775
0.90
0.69
0.85
0.54
0.76
0.74
89--910 20-11
00..8862 0.60
0.57 00..3666
0.70 00..6759
0.60 00..5694
0.68 00..6438
0.67 00..7644
1112--1123 13-14
0o.s6e2 0.55
00..5523 0.53
00..5643 ols)
00..6690 00..6505
0.58
0.57
00..5597 0.55
1154--1165 16-27
00..5645 0.57
0.51 00..4438
0.46 00..5460
0.57 00..4455
0.55 00..4588
0.51 o0i.s46
1817--1198 19-21
00..5431
00..3490
00..2384
00..4325
00..4398
00..4464
0.93
0.81
0.79
0.77
0.84
0.92
2213--2235 25-27
00..9613
00..7559
00..6599
00..6767
00..7606
00..7878
0.76
0.67
0.64
0.66
0.68
0.64
27-29 2302--3326
0.73 1l08
0.66 oles
1.06
1.05
0.73 016196
0.68 01..8291
0.66 0.82
00..8664
114 0.66
3460--4403 43-47
00..5997
01..5088
0o.s9s7
01..0802
01..7091
00..9813
0.59
0.78
1.00
0.94
0.96
1.25
47-50 5540--5574
0.70 00..5623
0.52 00..7576
0.66 00..8622
0.70 0.83
0.69 0.75
0.63 0.58
0.79 00..6918
57-61 61-69 69-78
0.56 1.38
0.82 1.33
0.74 1.41
0.89 1.64
0.81 1.66
0.94 1:59
151
1.77
1.49
1.60
16a
78-89
1.59
2.03
1.97 2.06
2.45
2L.3a
TOTAL 31.6
28.6
30.2
28.4
30.6
1.8
jean 0.91 01.:7271 01..9033 0.83 00..776 00..7658 00..5698 0i.s5o8 00..5554 00..4581 00..3493 00..8748 0.66 0.68 00..8668 1.05 00..9795 0.92 0.68 00..6718 0.79 1.50 l2.e0a8
0.2
# Data are expressed as percent of dose excreted during collection period.
000043
.
5
Table 2 iEnxcRraettsioanftoefrToataSlinCgalreboInn-t1r4aveinnouFseces Dose of FC-95-14C (Mean Dose, 4.2 ma/kg)
PeCorliloedction (days)
00.-50-.15 1-2 2-3 43--54 5-6 76--87 58--910 10-11 112--1123 13-12 14-15 1165--1176 17-18 1819--1291 21-23 2253--2257 27-29 2392--3326 36-50 50-64
a _-- T2 E 5 thm ee --------
00..7014132 0.955 0.761 00..773664 0.636 00..556309 00..444718 0.524 00..331191 0.351 0.328 00..224961 0.228 00..132748 0.357 00..028888 0.266 00..257700 1.397 1.086
0.7-80 0.770 0.572 00..663004 0.595 00..442634 00..343060 0.407 00..227798 0.255 0.233 00..127746 0.130 0.0.220754 0.258 00..313796 0.140 00..316164 1.074 0.663
00..096199 0.865 0.726 0.599 00..365026 00..893121 00..354927 0.327 00..320410 0.280 0.265 00..127746 0.171 00..127905 0.280 00..219348 0.197 00..429200 1.562 0.957
00..090848 0.465 0.466 0.667 00..339957 0.434 00..330367 0.299 0.295 0.304 00..239693 0.309 00..215726 0.156 0.111 0.294 0.303 00..225054 0.181 0.260 0.646 1.768 1125
00..717778 0.682 00..665329 00..550328 0.522 00..431413 0.371 00..226949 00..320202 00..225427 0.190 00..111107 00..237265 00..228108 00..214399 0.587 1.352 0.452
0.8-77 1.032 00..761899 00..552798 0.635 00..440511 0.433 00..337724 00..227986 00..228735 0.164 00..117583 00..334001 00..232068 00..223870 0.499 2.028 0.12
TOTAL 14.0
1.
13.5 12.0
ns
13.6
bean 0.0.084492
0.795 00..665469 00..551707 0.588 00..442812 0.387 00..239760 00..321801 00..227726 0.187 00..112693 00..330121 00..226028 00..222032 0.526 1.530 0.833 12.6
# Data are expressed as percent of dose excreted during collectionperiod.
000044
EN .
a.
mes in Ray ce at 65 Dp aye four a p Sirle T-- acvevamous
Dose of PC-95-14C (Mean Dose, 4.2 mg/kg)
TT a rs m em e ea e e,
Tissue?
g/g
vg/g
19/9
ug/g
9/g
va/q
g/g
Liver va mae am mas wes mar ase
Pxliadsnmeay tsapnlgeen
CL2o.s 0%
os2.17 os
a02.37 100
1m 2.27 am
21.1005 110
aa2.05 10s
oe oso os os ods ome
ae2.21 srees
sReodreslmeaorsorCells 00..413 Mrems oss
00.5a9 ow
oosms o0.m1 oosms ous om om
oosses om
oase am
Testes sesicn e
03
3 os
03
om
os
om
on
eal om om om oz ots om
swostmeows 0.05 028 ods 021 ons ons oa
Bre
ole 03s 03s ous om ous ous
qBuraanitnitaantdeab(d<om0i.n0a8lugf/agt) weirneabadlosmoinaaslsayfaetd, buatndletvooelloofw
ctaorbdoent-e1c4t
wiansbrtaoionlo(w<
to 0.05
zotal casbon-14 concentration is expressed as yg equivalents of 7c-95-1/g.
ug/g).
000045
Lo
-t0-
Table 4 atPe6r9cebnatysofafDtoesre aPrSeisnegnlteiInntTriasvsueensous
Dose of FC-95-1%C (Mean Dose, 4.2 mg/kg)
le ----
Liver
236 ma 5.35 2.08 2495 20.8 5.2
Plasma
"2.81
RecdenBlnoeoSa 0.42
2.82
0.64
2.84
0.58
3.00 2.75
0.41 oa
2.64
0.37
2.81
0.47
Kidney?
0.27 0.22 0.24
0.30 0.27 0.30
0.27
Lung?
0.15
0.1 0.12
0.17 0.13 0.11
0.13
Testes
Spleen
0.10 0.09
0.02 0.02
0.1
0.02
0.12 0.10 0.10
0.03 0.02 0.02
0.10
0.02
C--
bata is expressed as percent of dose present in whole organ. tPaltaasmaisvoelsutniemat=e3o1f.3pemrlc/ekngtboodfydwoesieghptres(ehnt).in plasma.
DReadtabliosodestciemlalste=of26.p3ermcle/nktgobfoddyosweeipgrhetsen(t4 )i-n red blood cells.
000046
Cl
Figure 1
CumslataicvteerBarcsseitnigolne
of 1v
DTootsaeloCfarFbeo-n9-51-4Mein
Urine
"
tenHaaonseo,f 46.2Famrs3ng)
0 0211s
fa
:
i fo
gS25 s
2 9 = % = [ Tw * w Py
-
Figue 2
Cumslative atser
Excretion a single
of 1v
TpootsaelofCasrbeo-n-9l5e-Mtn
Feces
eanHaaDnoseo,f 64.a2cms/c)
3
i:
i,
i
iiS s
asia
8g3 =
2o
To
= = % w ww
Days ost ose
ua
-1-
Appendix 1 Determination of Carbon-14 Content of Dosing Solution
5cJ0aulsiutlbrparatileoidrquomttioscdrvooespriienpgeptiotpfoertrstadetdsd,,ir1etchmtellFyoCf-i9nw5ta-ot1e%crCouadnnotdsinign15gvmislaollsou.ftiAoSqniuxawasso1l0snawlmepraleneddawdsidietxch2,
ccsaoplrecrcueilcfatitcieodna.sctiwTvehireteydmaaotfdaeFCafr-oe9r5-sb1ha4ocCwkngraoilunrneAdapdpyaenndddeticexorumTniatnbielndegS,1e.ffthieTchieueCnidc/oy2se.a0nda,dmlmiunswiaissntgertehde
each 1.21
mrgatofisFC2-.905-m1l9Co.f
sTohliustioisn
acomnetaanindionsge0o.f5543.2UCmLg/okfg.FC-95-14C
which
is
2 1/1 micropipettor, Lab Industries, Berkeley, California. 2 AfqourasFoCl-95-y1a4s dfuoruinndgtsopbeeciaficsuaictatbilveitysodlevteenrtm-isncaitnitoinlle(n1t).
dsepteecrimfiincataicotnivihtays b=ee0n.45r9epourCti/endg.(1).Specific activity
000049
..
"a=
Appendix Table1
Carbon-14 Content of Dosing Solution
4C1/2.0 al
Ci1/2.0 al
0.5602
0.5624
- 0.5554
0.5532
0.5614
0.5290
0.5628
0.5390
0.5612
0.5382
0.5554
0.5534
a LL Overall average = 0.5526
00.-5455386 ui /ng'. 1.21 mg FC-i951-h"C/2.0 ml
000050
ety
roe
Appendix 2 BiRoelcoogviecraylofSaTmoptlaels CSpairkbeodn-w1ihthfroFmC-9B5l-a1n4kC
Tshaemplgeosodharsecboeveenry roefporttoetda.l c(3a)r.bonS-i1n4ce frthoem ccaormbbounsteldabeF)C-8I0s7a-14tCo tihne bisoullofguircal
amteottoeamxbpoielncittbeostthhaitnFCbg-io9oo5ld-o1gri4eccaaolnvdersyatmhopeflecsotmotpcaoalnnenbcteasrabtootnfa-i1FniCe-d8f.0r7o-m1F4oFCrC,-e95ai-ct1h4issetarneodaf/soornabIltes
sFaCm-p9l5e-sT,4condboussitnegd,Solfuotuironrewpelriecataelsiqouofte1d0 wuilt,h5c0aluii,braatnedd 1m0i0cru1opiofpetdtiolrusted
dasniodrleutc1it0ol0nyulainndwtorpeispcaeitlntiteqisul,oltaetediiotnhdeirrveiactlthslr.yee ioAnrttoftchoeourmbsuarsmetepiloitnciamteceosnuessoinfwgh1it0chheyl,aslar5m0eeaduly
choomnotgaeinnaetdesb)l.ankThbeiolcoogmibcuasltiomnatecroinaels w(efreecald,rieldivaern,d mtuhsecnlep,elolretiszpeledenwith
h5anaddc.nbeateshnheleassdosdlevdefnitdlsitreerccotlpllaype.ecrt.edOne"iBnleaatnchkhe fovifilatletsrhetpoa1p0ewrhuli,cphel5l0tehteusl,FCw-ea9rn5-ed1hc1o0m0buulsteFdc-95-14c
esnpdikeodf epaeclhletssetweofrebirooluotgiinceally csaommpbluesst.ed aAtftetrhecobregriencntiinogn, fmoriddblaec,kgraondund
amnedancoruenstulitnsg effrfoimcideinrceyc,t apedrdcietnitonraencdovecroymbuwsatsiocna.lcuTlhaetedmeabny croemcpoavreirnyg
data
days
for
for
three
total
sets of fecal samples
carbon-14 (FC-95-14c)
that were
are shown
analyzed on
in Appendix
different
Table 2.
:
Tthhee mmeeaann orfecofvoeurrydeitser9m8i.n0at+io1n.s5,3. thiSsincmeeaneacrheceonvterryy iisn bthaesedtaobnle36is
d(entotermsihnoawtni)onwsa.s alRseocov9e82r.y ofThutso,talgoocdarbroenc-o1vheryfroofm stoptiakledcatribsosnu-e14homforgoemnates
Fe-35-1% spiked biological material is shown.
000051
oe
10m
Appendix Table 2
Recovery of Total Carbon=14 from Blank Fecal Homogenate Samples Spiked with FC-95-14c
---
_ 10 p12 750Mul t100wul
be
97.4~=
99.5
97.7
"100.6
97.0
98.1
.
97.9
95.4
98.1
98.6
97.3
98.0
Overall average = 98.0 + 1.53 (mean * 5.0.)
2 Amount of Fe-95-'c reference solution added. =b Data are expressed as % recovery pn from combustoiorn tion x 100) Each entry is the mean of 4 combustion recovery determinations.
000052
oe
[TR
Appendix 3 SamplesCboympaOrxiisdoantioonf VCearrsbuosn-1D4irAencatlyCsoiusntionfgUirnineAquasol
aTrhee buarsiende ocnardbionr-e1c4t ceoxucnrteitnigonindaAtqauarseoplor.tedHoiwnevtehri,s that an insoluble metabolite or complex might exist
trheeproertis a concern in the urine so that
cntoohnitsceapnloltsrsaoitfbiiotlnhietdyar,taadicooobantccateiinnvteirdtaytiforinnomdtdaheitraeucrftirnocemocuionsmtbicunosgunttioefodn.twheerTeosamiceonmvuperasirtneiedgatteo
irssaemsapullevtsas.lifdrTomhmeettdhhaoetdacotmaopraearsisssahyoonwntoistianl0A.9pc7pa.ernbdonTih-xu1s4T,abinldierteh3ce.tuTrchioneuenmtesiaanmngplrieanst.Aiqouaofsolthe
000053
Sh
er
Appendix Table 3 byCoOmxpiadraitsioonnovferCsaursboDni-r1e4ctAnaCloyusnitsinogfiUnrAinqeuaSsaemlpdles
Se ample rs er Diree ce t Counting-- Oxta-- ized -- OxtatzR-- eadt/io ee r Namber Cug/-- an)e---- r (ugE/g)E imDierrectt SCoOunRtiEngS
.
0.98
0.95
0.97
2
1.04
101
0.97
3
0.76
0.75
0.99
4
0.66
0.62
0.5
5
0.86
0.72
0.80
6
0.77
0.84
1.09
Hean
0.97
2 Data expressed as ug FG-95-"C equivalents/al.
daca expressed as vg eess-Me aquivatente/s.
000054
. co
2-.
Oistribution Lise
M.T. Case
218-2
Fp S.F. Chang
.218-2
We. rotor
---
eaeaeaa g
L.1. Harrison
218-2
- T.L. Kerley
218-1
J.D. LaZerte
236-1
Ra.L.J. tMealgisloln
21223-65
nt. ober
ae
1. Pendergrass
202
AL.R.A. PRrooskeonpchr
203236-38
Fh. thet
ae
P. Venkateswarlu
236-3A
TDeMcGh CeCnotmrnail caFitlieons
- 218-2
Central Files
218-1
000055
