Document 375nd5pjYLmQ1n7dR80VJdL50

ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206 or L3243. (1-Octanesulfonaiccid)(CAS #2795-39-3). Remarks: The 3M productiolnotnumber was 501. The testsample isFC206. Currentinformatioinndicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether,78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylene monooctylphenylether,and 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations ofimpurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute flow-through GLP: No Year completed: 1978 Species: Lepomis macrochirus Supplier:Commerical supplieirnBrady,NE. Analyticalmonitoring:Temperature,pH, and dissolvedoxygen. Exposure period: 24-hours Statisticamlethods: Notgiven. Test fishage: Juveniles Length and weight: Average length 30 mm Loading: 0.31g/L Average weight 0.25g (wet) Pretreatment:None Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutiownater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:Notgiven DiluterFlow rate:Not given, Concentrationsdosing rate:Continuous. Stabilitoyfthetestchemicalsolutions:Not noted. Exposure vessels:Vesselscontainin1g6 L testsolution. Number of replicates:1 Number offishper replicate:20. Number ofconcentrations:fiveplusa blankcontrol Water chemistryduringthe study: pH range(0-24hours): 7.9- 8.0(contreoxlposure) 8.0 - 8.0(2000 mg/L exposure) Temperature range (0-24hours): 18-L; Dissolvedoxygen range (0-24hours): 7.3- 9.0 mg/L (controelxposure) 7.0- 9.0 mg/L (2000 mg/L exposure) RESULTS Nominal concentrations: Blank control3,00, 600, 1200, 1800, and 2000 mg/L Element values: 24-hour LC5o (FC-206 only)=>2000 mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on the mixtureas described intheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 24-hour LC50 forbluegilslunfishwas determined to be >2000 mg/L. - Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordofmethodology used. The sample puritywas notproperlycharacterizedand the studylacks analyticaclonfirmatioonftheamount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, 1978. OTHER Last changed: 6/28/00 ENV)RONMENTAL ENGINEERING LABOPATORY AQUAI'IC TOXICITY DATA SHEET l'ypeTe!;t-P-4-A- Mate ial Teste(f Lot ,IstOrganism Avg. Wt. Sklji;lmVendor F4c4 grams Avg. Size__ Date Recld tO -1"1.7latch No% cm. Test Solution Volume Liters/Tank Time Started__I()I.,'?D a,,,Date Started__IL-_LO No. Organisms per dose Date Completed 2c3 Dilution Water Carbon Investigator !Aa.Lo, (Full Signaturci) filtered well (Temp. water oc D. 0. lf&Ufc so (gs% to'nfiden:deLimits)' o ppolp,it Conc. mg/l. Total kmount Added Control ?4-Hour Total D.O. iNo. Dead pH lppm 48-Hours Total No.Dead PH D.O. pps 72-Hours Total .0. N'o'.Dead plildppm 96-Hours Total ID.O. No.Dead @H ppa DO-0 1 10 10 o @ltigoe-ol -7,917.2 - Lb '72 Ab 20 Test Temp.oc 3ignature & Date Comments: Effects other than Mortalit), ConE-.- Symptoms or Reactions mg/i Control 24-Hr. S*o Total No. Affected 48-Hr. 72-Hr. 96-lir. ;i&,..Ature & Date Comments: