Document 305KoYXOJpK6BdzQ0M01yY5J

A R SlA 6 _ 0 8 7 / BIODEGRADATION (OECD 301D) I ES31SUB5TAMCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-780B. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 2. The test sample is FC780B. Current information indicates it is a mixture of 0.58% PFOS, 14% diethylene glycol monobutyl ether, 76.7% water, 5.5% urea, 1.17% Hydroxy foamer, 1.15% sodium octyl sulfate, 0.8% polyoxyethylene monooctylphenyl ether, 0.05% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data does not accurately reflect degradation potential of the fluorochemical component of the test sample. METHOD____________________________________________________ Method: OECD Closed Bottle Test, OECD Guideline 301D and C 117/79/GER Test type: Ready Biodegradability GLP: No Year Completed: 1980 Analytical monitoring: Dissolved oxygen depletion Statistical methods: Results were determined by calculation of the % BOD/COD over time Test organism source: Non-pasturized field soil obtained from 3M Agricultural greenhouse, St. Paul, MN Test condition: Dilution water: Deionized water and distilled water. Reference and test solution preparation: The dilution water was prepared using Hach BOD Slurry Pillows. Nitrification inhibitor, Hach Forumla 2533, was added to replicate dilutions of blanks and samples at the recommended concentration of 0.1 g/300 ml_. Test vessels: Not given. Number of concentrations: 1 plus 2 reference substances, and blank, all in duplicate. Temperature: 20C Total suspended solids and pH on day of testing: Not given. Element Basis: Decrease in oxygen depletion compared to the blanks. 002534 RESULTS Nominal concentrations: Blank control, LAS and sodium dodecyl sulfate (reference substances), and test material. Element values: 5-Day BOD/COD: 15-Day BOD/COD: 28-Day BOD/COD: 21% 78% 72% Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS__________________________ _____ ______________ The test substance % BOD/COD was 72 after 28 days. The % BOD/COD with nitrification inhibited was 83 after 28 days. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability. Klimisch ranking = 3. Study lacks description and records of the method followed. The inoculum is not properly characterized. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES__ ._____________________________________________ The study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6211S, 1981. OTHER_____________________________________________________ Last changed: 6/28/00 002535 I f - f (c lU S Tost Guideline C U7/79/GSR Tost Guideline for the Closed Bottle Test acc. to Fischer Date of last revision: August 27, 1979 Level I test for ready biodegradability Prerequisites It has to be known whether the test material is soluble in the concentration range employed in.the test (2 - 10 mg/1). If it is not, special measures such as the addition of solubilizing agents, ultrasound dispersion etc may have to be applied. 2. Guidance Information Knowledge of the bacterial toxicity or inhibitory proper ties of the test material is not unequivocally required but constitutes useful information. Qualifying Statements The test is applicable for the biodegradability evaluation of organic compounds but not for mixtures thereof. .Most experience has been gathered with water soluble compounds, however, the test lends itself, at least in principle, also to the biodegradability evaluation of volatile and insoluble.compounds. ' 9 002536 August 27, 1979 OECD Chemicals Testing Programme Test Guideline for the Closed Bottle Test acc. to Fischer 0. The test procedure is based on a paper, by W.K.Fischer, P.Gerike, and R. Schmid in Wasser- und Abwasserforschung 7, 99 -118 (197'0. 1. Method 1.1 Introduction; Purpose, scope, relevance, and applica tion of test and explanation of limits* The purpose of the method is the measurement of the biodegradability of organic compounds in an aerobic, aqueous medium at a test*concentration of 2 (standard concentration) to 10 mg/1 of active material. Most data elaborated with this test pertain to water-soluble compounds, however, volatile compounds and those of low water solubility may also, at least in principle, be tested* 1.2 Definitons and unit3 v 1.2.1 Definition of biodegradability mg 0 / mg AS % BOOT = ------------BODT mg 05 / mg AS % BOD/CCD= ------------------------- mg COD/mg AS -O 002537 ^ # 0 * 1 4 1t W l- M O V-- :V&'"<*.w - File: Page Nn.^ ENVIRONMENTAL ENGR. LAB - WORK SHEET J "* SAMPLE DESCRIPTION Date: ~3 / _______ Analyst: fn A **jP Hrs.: ______________ ________ / T ^ t CY H A C rt V U s i x . / &Qr i J t l G\ \ ) sC*J~ZL4-^ 2> <s & .a ^ , P M m v , IIaXa. ^ A ^ tC n ^rrn . f ld M d r ____ T > A t & '& ^ A , .* /t __A ^ H A C H f fiM U L A 2.^33 y ..... < S^ L ^ e ^ s& il s m j J U * - ' s f l ' / j / 'Sq ^ - Reviewed by: _________ __ 002538 3M ENVIRONMENTAL LABORATORY Form Sheet f o r th e C losed b o t t le T e st Lab raq u ast n o .:_ 6 2 1 I S <b I S ^ a r ? 'D ate o f s t a r t o f t e s t ; 1 0 " 3 1 " 8 0 _________A n a ly s t: V . A . S c h e i l Test / standard m a te ria l: OOOT o f t e s t m a t e r i a l : ________________ mo 0 7 /mg AS Temp, o f th e d i l u t i o n w a te r a f t e r a e r a tio n : T h e o re tic a l te s t co n e .: COO o f t e s t m e t e r l a l : _ _ 0.0 7 221jl. ( 20 o R e f . : OECO, Tokyo,I2-01-7S C l17/79/GER /< A 7 mg A S /1 (AS a c t iv e s u b s ta n c e ) mg Q i / mg AS 0 ,-co n c. o f the d is t ille d (d e io n ize d ) w a te r a fte r a e ra tio n and s ta n d in g b e fo re the s ta r t o f t e s t : 8 95 wOl'1 O ls s o lv e d Oxygen a n a ly s is ; Oxygen e le c tro d e method c a lib r a te d a g a in s t th e W in k le r method Inoculum : U n fe r tiliz e d garden s o il A : 0 , DETERMINATIONS: B : 01 DEPLETIONS (mg B O O /I) AFTER X PAYS: B00x - (m0 - m ^ ) - (mQ - m ^ ) * MOOI F I ED FORMULA: BOO. (.i-. ) - (".o*s^) * T h is d iffe re n c e Is Im p orta nt as a check fo r the v a lid it y o f the te s t (see 1 .6 .).1 .3 ) . C : EVALUATION: The m o d ifie d fo rm u le uses th e measured I n i t i a l 0 cone, o f th e te s t s o lu tio n s and bla n ks In p la ce o f a ^ . X BOOT - mg BOOx / l mg A 5 / 1 * BOUT ' BOOT, 100 days S * ,M IK OR X BOOg/COO 2R mg I 0 0 x / t 100 mg B O O */! a f t e r X d a ys SI IS f 18 V8Q0/C00 /> h /c> ft Y N i f o l f v o t . COMMENTS: A L - _JZ 6 _ ?a_-- AO S' 3 .^ 7 lt A *, "te r JL . jo b * ! 0l U aH , . 62 n sLab requast n o .: 3M ENVIRONMENTAL lab o r ato r y Fo r S heet fo r the C losed b o c tla T e st Ref . : OECD, Tokyo, 12-01-7 c 117/ 79/GER D ate o f s t a r t o f t a s t i 1 0 ~ 3 1 ~ 8 0 T e s t / s ta n d a rd m a t e r ia l: QQWCL * " y t s___W . A . S c h e M So^turt Sfy-7~ r h a o r a tlc a l t e s t c o n c .:_ BOOT o f t e s t m a t e r i a l : mg 0 ? /m g AS COO o f t e s t m e t e r le ! ; _ Z . O Temp, o f th a d i l u t i o n w a te r a f t e r a e r a t io n ; 2 9 3 K__________ ( 2 0 o Z.O mg A S /I (AS a c tiv e su b sta n ce ) mg Q s/ mg AS O j-co n c. o f the d is t ille d (d e io n iz e d ) w a te r a ft e r a e ra tio n and s ta n d in g b e fo re the s t a r t o f te s t ; -8 z 2 S . JMJfr/1. O is s o lv e d Oxygen a n a ly s is : Oxygen e l e c t r ode m ethod c a lib r a t e d a g a in s t th e W in k le r method Inoculum ; U n fe r tiliz e d garden s o il A : 0-, DETERMINATIONS: CULTURE MEOIUM Minral n u trie n t solution w tthout t e s t m a t e r ia l and w ith o u t Inoculum. Mineral n u trie n t solutio n wlthout test m aterial but w lth Inoculum. Flask no. Analyses i 2 3 k* cS ro l 1 2 3 I* * mean blank cl C3 Ck * " C l C2 n Cn B, 2 3 Bk * " b " * | 2 " Bn n 1sg O j / I Alf TER X OAYS 0 5 15 9 .1 0 8 .6 5 8 .5 0 9 .1 0 8 .7 0 9 .1 0 -8 .7 5 9 .1 0 r 8 .7 ^ 8 .5 5 8 .5 5 8 .5 0 * 9 .1 0 8 .7 0 8 .5 3 9 .1 0 8 .7 5 8 .5 0 9 .1 0 9 .1 5 9 .1 0 * 8 .7 0 8 .7 0 8 .7 5 * 8 .5 0 8 .5 5 8 .5 0 * 9 .1 2 8 .7 2 8 .5 2 28 6 .9 0 7 .2 0 6 .8 5 8 .2 5 * 6 .9 8 6 .8 0 6 .9 0 7 .10 8 .2 0 * 6 .9 3 *V a1ues n o t in c lu d e d th e meai v a lu e s ; th e s e d lu t io n s c o n ta in n 1t r i f i c a tio n in h ib it* . /* Mineral n u trie n t solution w ith test m aterial and w lt h Inoculum. i 2 r* ~ k 'mean test m atarla! A. A2 * Ak mt A | a A j e n ? ,/* i'9 0 9 ,t< > (p* 3 0 r , 7 o 9^ O S '* 6 . 3 0 * . 9 ? * 3O * * S e e n o t above. q jo . I ? s '. r r 3 .s r B : 01 DEPLETIONS (mg B 0 0 / I ) AFTER X DAYS: 800x - (m0 - m( J - ( D - m ^ ) * ' T h is d iffe re n c e Is Im p o rta n t as a check fo r the v a lid it y o f the te s t (see 1 ,6 .3 .1 .3 ) . C; EVALUATION: % BOOT - mg B00x / l 100 /. BOOTX 7BOO/COO /` ft *P/C,oO COMMENTS: a fte r deys 5 IR "< ? 1 k i Ip, - h e J . 7? * *o -* '# *H 0 0 IF IE 0 FORMULA: BOO. - (m ,, - m, ) - ( * t - s v ) * The m o d ifie d fo rm u la uses th e measured I n i t i a l 0 cone, o f th e te s t s o lu tio n s end b la n k s In p la c e o f mg. OR % BC0g/C00 - mg BOO/I mg 4 / I COO 100 R 76 mg B O O y/l a f t e i X days 5I 15 28 2 ,3 ^ 1 2 .^ 3.0 002540 3M e n v ir o n m e n ta l labor atory Form Shoot f o r tha C losed b o t t lo T o st Ref . : OECO, Tokyo,12-01-7 C 117/79/GER Lab request no.: 6 2 1 1S Date o f s t a r t o f t o s t : 1 0 - 3 1 ~ 8 0 A n a ly s t: W. A . S c h e f l T e s t / s ta n d a rd m a t e r ia l:. / I S REFEA t d e e SAMPLE T h e o r e tic a l t e s t c o n e .; 2..2MBOOT o f t e s t m e t e r la l: ^mo 02/mg AS COO o f t e s t m a t e r ia l: . V S " mo A S /I (AS a c t iv e s u b s ta n c e ) up q, / mg as Temp, o f th e d i l u t i o n w a te r a f t e r a e r a tio n ; 293 K f 2 0 **C) 0 j-c o n e , o f the d is t ille d (d e io n ize d ) w ater a fte r a e ra tio n and s ta n d in g b e fo re the s ta r t o f te s t: JL.2. "a Pa/ D is s o lv e d Oxygen a n a ly s is : Oxygen e le c tro d e method c a lib r a t e d a g a in s t the W in k le r method Inoculum : U n fe r tiliz e d garden s o il A : 0- DETERMINATIONS: CULTURE ME0IUM M in e ra l n u trie n t s o lu tio n w ith o u t te s t m e te rla l and w ith o u t Inoculum . M in e ra l n u trie n t s o lu tio n w lth o u t te s t m e te rla l b u t wl th in o c u lum. F lask no A nalyses 1 2 3 Me mean c o n tro l 1 2 3 kk mean b la n k c, c* C3 C|, * n C | C2 . . . c n n V 2 *3 ' "S " it* B | B j . . . Bn n 1g 0 j / l Al'TER X DAYS 0 S IS 9 .1 0 8 .6 5 8 .5 0 9 .1 0 8 .7 0 9 .1 0 -8 .7 5 9 .1 0 r 8 .7 0 8 .5 5 8 .5 5 8 .5 0 * 9 .1 0 8 .7 0 8 .5 3 9 .1 0 9 .1 0 9 .1 5 9 .1 0 * 8 .7 5 8.70 8.70 8 .7 5 * 8 .5 0 8 .5 0 8 .5 5 8 .5 0 * 9 .1 2 8.72 8 .5 2 28 6 .9 0 7 .2 0 6 .8 5 8 .2 5 * 6 .9 8 6 .8 0 6 .9 0 7 .1 0 8 .2 0 * 6 .9 3 .c *V a 1ues n o t in c lu d e d th e m eai v a lu e s ; th e s e d lu tio n s c o n ta in n it r if ic a tio n in h ib it! M in e ra l n u trie n t s o lu tio n w ith te s t m a te ria l and w lth inoculum . 1 2 3* It < mean te st m a te ria l A. AJ mt - A | A2 . . . A n n ? ./* 9 , /o f,o r> ,bO * tO r to CSO * 3 *7 0 q .s r y. * 7 , / o Y , ( f Q i O 9$ y ./Z 8: i DEPLETIONS (mg 800/11 AFTER X PAYS: ex ("0 * ,x) - (" 0 * T h is d iffe re n c e Is im p o rta n t as a check fo r the v a lid it y o f the te s t (see 1 .6 .J . I . J ) . C j EVALUATION: MOOIF I ED FORMULA: BOO, ( .- ) - (% -% )* >e " O The m o d ifie d fo rm u la uses th e measured I n i t i a l 0 cone, o f th e t e s t s o lu t io n s end b la n k s In p la c e o f m0 . % BOOT - mg B 0 0 ,, / l * 9 A 5 / I * BOOT /. BOOT, v a q d / coo Y'/ 'f>o/(roo K COMMEENNTTSS; ' 100 OR %BODg/CQO a fte r days 5 1 0.9 93 JR 99 0.6 3 7 JtE.______ SI r \ L t i / " * , mmgg IO7O1x/1. COD 100 5 mg (8dOx / l15a f t e jr X d2a6ys \Z , 7 f * S e e n o t above. 2. LAS g Y i* L A S A tm * 'J l X u 3/0. * ^ Zi / tas&l = 3fZj K S 7 ( ? |& fc pT R V<,: r L --- __ .7, .i --l Q 31Z < CL b fi j j ____ a m*3u~-P'ytmfiJ*-' n~w l v <:xi mV?W\ M JUT*bajyM*'fta&/pf*r nyyyrv <vtfoy<yr; vS' a m ifW g a g 4,:,.- *\; 1 B : i iA-.,-. ,' '' - - .. 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J5FLt'L'KL3/ JJJO`0TTb/ 3/ *hi fY+yp^fTv^tr^pyjr^ry^yrw^Vr`yyoq-&>tjr7Uf yJyV^r-WO`Y517'" S f E 2 J lLJ----------------------------- V - J 3 3 W ---------------------- 1 / yy / A ? -rP/ / A/ / / / / r jf/ Z ____ L .... ____ L ___U & -L 133HS ( 31 0 ~ y ^ z NUOd S ISA1 VNV J'* 4 ? ---------- ------------------- rw a ^ ; jS A ^ ( \iy 5 M r 9 # ^ 7 3313 W S "trp P Q ' -e ^ iv^r v-SQ*9 wOJ F O R M 1 0 6 2 0 ` 8'PW O BEST COPY AVAILABLE File: Page No 6 Z l( ENVIRONMENTAL ENGR. LAB - WORK SHEET SAMPLE DESCRIPTION , i) U U L J I I *5 Date: f ~~ 3 f ~ f f o _______ Analyst: J J .- 3 Hrs.: ______________________ ____________________ L X - h r * . P xJU svJf2* - '____________________________ . lKA^T>\y v \ J ^ S ^ -P `^ .o A r ' T) i H a 0 ! M s t r > u t^lJfl^.) ^l^ v C L ____ __ y U c Z h s L itw-P .i . jx J O . Da^, 9^/ &V rw ,ir - ffyo T>s z s * f,o o ________ *73 -79 9. t .t o tf.-S'O 6 ,g T ' r .i* fty r t 9* . O %,/0 ^ m iJ 06^ D / &4>4k \ ^ 2 o ( JjL / b tc ^ p JL ) IaT C & K j i /u iJ a s J ^ J a 0 TM J l U n Z l L * jiX lU t a (1t1 41 7? 11 _________ * 6 * V* (c>^ < $ co 9,/0 . *hoo 1 ,1 0 ' f,/o 9, /o ~$/jr mo Z-3o f,3 o ( y 7?us & ji/xU ^ --0>tft$--?---- -- j cm eu% % g . 3 ,3 s" f'3 o J ______ fcWf c f i . - 6 Z Q t l f O A C j / 305 ^ . -v/:, Reviewed by: 002543 . :T>:r- iik . " iS r . t F O R M 1 0 6 2 0 ' 1 `PWO b e s t c o p y a v a il a b l e L .(Z ^ File: Page No. 6 2 . S ENVIRONMENTAL ENGR. LAB - WORK SHEET SAMPLE DESCRIPTION -ri* stt, 1.\~ t' - o e c j T ^ . Date: / 0 - H - 0 Analyst: Hrs.: _____________________ g n't h t T a s h i a* / -{-r*t- - jo -TI-fro i l ) )e/teyJt _____ AP*. # g c y teU uefrO ic tt. ( ^ lu r y O su U v6 ^ AbuA /M i**, i.j , H) fc"*), . O S O . , . ____________ _ ______ (%) L-fo 'kf&bsuoe yd, ' '- lit * X i/ i/. )&. Ay j f r W i g m Q M L , /J k c - C J i M ^X/L 'fit y ch*^ T . ' " lei/ 3iy .fo* -R e v ie w e d b y : - 002544