Document 2yergERkgBrE81mBO3XyRkj6

/)R Z X 6 -0 I MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE_______________________________________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The test sample is FC-143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 100% test substance and 0 - 3.5% C6, C7, and C9perfluoro analogue compounds. The 3M product lot number was 427. METHOD:_______ '__ _____________________________________________ Method: Microbios Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1996 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: Ajprimary 2000 mg/L stock solution was prepared in Millipore Milli-Q water. The pH of the stock solution was then adjusted from 5.8 to 7.6 using 1.0 N NaOH and an osmotic sadjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure vessels: 3 mL Cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss RESULTS_____________________________________________________ Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L Element value: 5-minute EC5o = >1000 mg/L 15-minute EC5o = 800 (790 - 820) mg/L 30-minute EC5o = 730 (630 - 850) mg/L 004022 Element values based on nominal concentrations. CONCLUSIONS__________________________________________________ The FC-143 30-minute EC50 for Photobacterium phosphoreum was determined to be 730 mg/L wotj a 95% confidence interval of 630 to 850 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY ______________________ ____________________ Reliability: Klimisch ranking 2. Testing meets ail criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES___________________________________________________ This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number P1626. O T H E R _________________________________________________________ Last changed: 5/25/00 004023 BEST COPY AVAU ABU ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET V' ''VOif'v^r ' LR: P i*3.0 Date: `i - 2 `4 'HoW: Analyst: - TEST SUBSTANCE: F c r l M 3 *-c t H V 3 - PO o f IV J t # -'-K; 1. Preparation of Stock Solution loom91toOr'O Mass/Vol. Test Substance: 3o ins\ ffr r^p >-2^-74 *Final Dilution Volume: a rA fL * *Diluent: IY\.\W - Q o&g&r ? :n * Final Concentration of Stock Solution: o \ L. 2. pH Adjustment of Stock Solution *InitialpH o fStock Solution: S - S' *pH Adjusted to: 7 <o *pH Adjusted with; '- - ' ? . > 3. Osmotic Adjustment of Stock Solution : Osmotic Adjuster: /- ^ .H>a' , ' ' . . - ' > ' i .'-. . f . v 1 00402 BESTCOPYAVAI l a , '' 1 % PCJi o f ir;.' f Il e r-c-i4i.k3 R1626 tot 427 MICROTOX DATA REF'ORT Basic Test ce* p i T*r$rb Test Times S nunutes NUMBEP jn /ir *, 5# O s m o t i c A d j u s t m e n t x N_Va0C<,l.J3S.vt00 CONC. C R / G A M M A 1Lo--s-- Lobj ' Contrai' i/t.'rr. BQ.m Control 93.23/ 8 8 . i ... 1 ' 99.34/ 8 8 . 3V,,.^; SI .89/ 7 9 . o S 't?-,'-. 92.*?/ to.95 RI.07/ 68.35 9'-.?,?' 73.39 V ii. AV 7 2 , 8 6 .... . ? '. .4 -' 4 9 ,2 o v > j n y i . , 1 ,>. . ''1 .9 '/ 44.714 ilOOO.o 1 'o..CRf ft u icd far- t -.C50052A 103 MG/U 04025 J;:.cI. 'j ; l / f j i j *' 1 1 * 11 m o t i >t ; i ' : ; - ' i l j 4 ?J. - J O j ' J " 1 - ! i-.::l j T 4* I a ltre ; S- o n -'Mo),*'or 'W&'-.i r t f -Afsf.v / * m ,a , fe |m * aio'o-r fe5,geef>r-* vm&F*T? 9fW*0 '# I l 'O ( I I ' I '<_> ' ' . J O ' J i 0000'1.100 I 0000OK Oor .)oos: cuoo-osz 0 0 0 0 *OCS- (.1000' '.2 0(000"SST 0 o b -T i f*v *9 c 0 ( 'fr 9 E 'T O 69 T 9 S 9 -* / S S " ZZM B / / 6 ' lo /fr< y *.6 / 'Q i y ? f.- 6 b /; 8 ` 1B / 0 6 ' ''6 / 6 -T li / b C '6 6 - 0 8 `9 6 0 '9 8 / Z * A / 9 '9 6 9 c; V 2) l. r;> j}u -\i3 1c o in e r) T ino:) 1 1 /0 1 d s e ^ riN 00 mi?N:uann5nrptf V T O i U O V.r-' i n t l 1 l < } - . *U> t i S o X 4>v1 Mix., atsK ' ' :n K | idOdrjy vwo xcuodoiK . r . t >'"n 9 ' 9 t d T r t H - J d 3033 m w m m im BEST COPY AVAU ABU FILE FC-143.K30 PI626 Lot 427 MICROIOX DATA REPORT Baa ic Tes*: *f c l i UtL * f >t>X<a- \ e s t Ti-iiax 3 0 ' m irili te a NUMBER 1 0 /IT , , C o n tro ] C o n tro l 9 6 .6 7 / 9 3 .7 6 9 3 .2 3 / 9 1 .0 6 :. '-Ts A ! . .: - 9 9 . 3 4 / . , 0 6 . 1 8 2 r B 4 -.e 9 /,'e 2 .6 ; 3 9 2 .0 0 / -7 0 .0 0 4 " 8 1 . 3 / 6 6 .1 0 - . 5 6 A,, A 9 9 . 3 2 / 6 7 . 1 / r f 9 S .9 0 /. 6 6 .9 4 7 - ' ' 9 9 .6 4 / 3 3 .0 0 5 ; ,91 . 9 ' * V 3 1 . 0 6 , . O s m o t i c A d u s t ^ e n t ; N.-iC 1 CONO. tv. . OHV / 30* ir* L >q K t M l- o d .0 .0 0 .0 0 . 9 6 9 " . +* -- 0 . 901.1 1 2 5 .0 0 0 0 1 2 . OOOii k Zj O . C O O O 2 r '. 0 0 0 0 3 0 ' i . O O C 0 , 500000o 1 0 0 0 . 000*.i 10 < ' 0 - '`> 0 0 0 o.i / ; . O l i >. ...09 ' . - I 0 *.*. 4 4 4 i ,. * j H T* u u M .3 O i .i IJ 3 3 o sr 3 0 .3 LU I 6 V (a ` . cyc L i a M LPVS Y; S .C ,'.0 O h - ' V 11' , i5 a - , 3i>l fcl 3 C R '= C o C O R f x t C T ICN'V F A C T O R ' } '. 9 7 7 r 4 -for c a l c u l a t i o n s ' * 1 .. V . 1 . - * ` ' f r.J > T? 1. ` i'j ? 3 0 . 8 6 O o M C / U ( 9 3 % ' C O N F E N C E R A L L E : <5 l i . d a . : . v ; :. , .' 5 0 0 ' - <\ i Slanature S' TEST DATE : ' ^ Vtr TIME: ! > ^PPPDr.DOODDDDDDOODDOOD.WOVOPDVDUPVVDPVOPCPOOPOJi 3>uflFt = 1,6713. '~`i So . 3~J$ / sjr4V-?LAtV4tI4 V -.te? famxrsf' jz y * >*fa*r K< % ICO ^ n ^ r X f j + ) \^>c POP js ir -A' .r,W^ i mm , -v ; --f 0. T.A r ' ; a -/'-1. . a M : s, , "& Ti k$<yifyb$*