Document 2qxab2kDpavNOJo2ojvdwBg5r

AR 236 -- 075] TOXICITY TO AQUATIC PLANTS EST SUBSTANCE ---- Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot numberwas 519. The test sample is FC- 203CE. Current diethylene glycol information butyl ether, indicates it is a 60.23% water, mixture of 1.84% PFOS, 30% 2.56% Suitone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene `monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyitriazole. The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD: ---- Method: OECD 201 and ISO/DIS 8682 Type: Acute Static GLP: No Year study performed: 1989 Species: Selenastrum capricomutum Source: Originally obtained from ATCC, Rockville, MD, maintained in culture medium at 3M Environmental Laboratory, St. Paul, MN. Element basis: Algal cell counts (cells/ml). Exposure period: 96 hours Statistical Analytical Methods: Not given. monitoring: pH and temperature. Test Conditions: Algal Nutrient Medium: Nutrient `medium per OECD 201 and ISO/DIS 8692. This nutrient medium provided all mineral nutrients essential for algal growth and also `synthetic servedasthe algal medium diluentfor all algal operations. was 7.6 adjusted to 8.3. One The pHofthis hundred mL of this pH- adjusted nutrient concentrate solution was added to 900 mL of Millipore Milli- QTM water. Final pH 8.3. Stock and test solution preparation: A 1000 mg/L stock solution was prepared by diluting 1000 mgoftest substance in 900 mL of Millipore Milli- - QTM water then adding 100 mL of the pH-adjusted nutrient concentrate solution. Final pHof this stock solution was 7.9. Aliquots were then added directly to dilution water in test vessels to create test solutions. 001097 Exposure vessels: solution and capped 250 with mL Erlenmeyer flasks containing stainless steel closures. 100 mi. test Agitation: Number of Shaken continuously replicates: 3for the at ~ 100 rpm. blank controls and 2 for test material treatments Initial cell loading: 1.0 x 10 cells/mL. NLiugmhtbienrgo:fc4o0n0cfeonottr-caatnidolness:frfoimvecpolnutsinauboluasnckocooln-twrhoilte fluorescent lighting. Water Chemistry: pH range (0-96 hours): 86..38-79..94 ((c1o0n0t0romlge/xLpoesxuproes)ure) Test temperature (0-96 hours): 24C (incubator) _ RESULTS preet----ieees Nominal concentrations: Bk control, 100, 180, 320, 560, and 1000 mg/L Algal Growth Response at 96 Hours. Avg. Growth Rate % Change in Growth Rate [BkContol| [100 | [1801 [320 | [seo | (Cao00| 1383 1.3308 1.2703 1.0891 1.0128 oso4s |-- -- | [33 83 | | 42] Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not given. R`Seumbasrtkansc:eTReestmianrgkwsafsielcdo. nTduhcetveadlounesarempioxrttuerde as described apply to that in the Test mixture and not the fluorochemical proportion alone. CONCLUSIONS Tchoemtpeasrtesdutbostthaencceonetxrhoilboifts4a29%6-hatoutrhepe1r0c0e0ntmcgh/aLntgreeaitnmgenrto.wth rate = Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 001098 DATA QUALITY Rteesltiianbgi.litHyo:weKvieirm,istchhersatnukdiyngla=ck2.s iTnhfiosrmsattuidoynmoeneptusritthyoefctrihteeritaefsotrsquubaslittaynce and the study lacks analytical confirnationofthe amountoffluorochermical proportion in the solution. REFERENCES -------- TSth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMG1C3o5m8p-a1n,y1,98E9n.vironmental Laboratory, ovWER Last changed: 6/26/00 - 001099 oo i: i% [: i3 J 5.: BE ee-------------------------------------------- ENVI1RONMENTAL LABORATORY 7S/i3r5)8sy STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE 23 fowl IFT nuTRIENT INCOSNTCOECKNTRSAOTLIUOTNION FI_INNALTECSTONSCoELNUTTRIAOTNION STOCK SOLUTION 11 MACRONUTRIENTS NcNaHotalCrLd.ioamn,i0o xa9502. ed;7H220 STOCK SOLUTION 2: Po-EDTA 11.35 9/L 5111.3586%g/Lg/t 115 18 mmgr/tL ng/L m= q 151.8n%o/L 8 3 oy a wFaeClE,da.6.H,300,0 18000 mmgg//LL STOCK SOLUTION 3: TRACE eLemeNTS nC. no coRcznacucgc11h122d2.o:6y218d,200no STOCK SOLUTION 4: Nahco, 3 melt 7o13l.mon5rgam/sgLm/g/LL wun EB 18000 uugg//LL 2> EB w3eugn/L @hn 701l.051auo"/Sto,n nea 2003400 NaHCOy 50 g/L 50 mg/L Cah S(tmeearnilipzoeresdtoicakmetseorlut0.i2onsum)1.-4 by membrane filtration SPOrLeUpTaIrOeN f5:casNhUlTyRIbEeNfTorCeONCeaEcNhTRAtTesEt.) pie Add 10100 10 maLL AL OOoFfFSSSTTTOOOCCCKKK SSOOLLUUTTIIOONN SOLUTION 127; 3). , - and 10mL ofSTOCK SOLUTION 4.41 CSiTanEER nSTRE take up to! SIET RRA lLeaddalg. , ator. SSvIReSin. : Mdjust SOLUTION 6: the S pH to18:3t 1310.7 5e p57 u "lo d Gy HoHo FER a STANDARD ALGAL NUTRIENT MEDIUM Di luaiCoetvu olt atsa Es 2409901000a-LaLooff=en eI choo' EpCiE air-adjnuTsEtT tedoNuaT rteient ConSceI ontwraatteer.SolutAionERa G Biss mmrmen aarlr l ((21)):7~\f O1CE8oC0oDpo ;a119r98a8r 7t4.i2o4Wni Ao1afagn:T adC{htDeee mvFeGiericolws caotrhplyyaset{t In.ntaOhyolrisi gbgPiaautnaliirn oczinaar.sto;g iTioowPnsrn:ta,nTfcone2rh.0i1bEr4ic2co1iinnoosnmsLiCTcuiuenrlsdtsdesiolUsifnSeSsARUSeMENpeRblEYssE:S L ;{ G1e80n/eDe v18e.: S86w9in 2t.z7erIlnatnedrnatFiEoEna TalEOvreganntiizatiLoine',foLreoSntandacdizDaiotdied iPsE A Nl Cr FN a Lab --------------N ENVIRONMENTAL LABORATORY L S5o5e] STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE p7z ayN NuRIENT ICOSNTCOECNKTRSAOTLIUOTNION IFINNTALESCTONSCOELNUTTRIAOTNION SS STTOOCCKOSOLLUTIOUN 11::TMMAACCI RROoNNUUO TRIENNTS aHanacctlhroind0 aso Tuo VL3s on 1890 11185 3mmoohsstt ainged, 01:1si6%G/rL 151.8m%ost @ STOCK SOLUTION 2: Fe-E0TA a NaFe,CBlT,A..6H,0,0 - 10800 SS sTtOoCcKkOsoL wrioUn 33::TTTRaAaI CcEe EO eLvEeMmEN eNnTtSs mmgg//LL - 80 100 uugg//LL. = o 9 WE. HBO, 0 zGcnouccc1i1}Z2.:62un,200 aa 185 mg/L 13 .m5g/mLg/L Sa > 185 ug/L x 31.va5/L/1 S=s Rshoy an 0.0a1rt8g/L 030a1% Stock souution 4: wanco, CR Hatcoy 0 g/L: 50 ng/L S(tneecainlipzoeresdtioackmetseorlut0i.2onsum)1.-4 by membrane Eiltration s{owrp ioneE5:czae wnolrymipbeenftoa rceonec acchouetresxt.) LiEHh: EF S2 pi7lo3 . Add ana" 100 111000 aL of aamLLL ooofff STOCK sSsttTooOccCkkK sSSsooOoLLLcUuTTUvIITOroNNIi1(Oo23N,nSLECRHAIoERHgRIi iANkGtNsAPRRLE YiR an E4aBalhe GGie5 EE aMakjeusutpehseo p1h.0t0-o0 mt8-w3ia 8th0.r 7LITa SlSeaLlayveE r mSCTda ES Gla o Bar ER 3 being p=:77: 2 SOLUT2IO00N,910600:0aSaLTtANoofDfARMDwilhlAoioLpGiAvLsN(ao rUr3TaitRnen lIiaEeludNgTNloHuEoDnTtOeLkWAAMcoFnOFtNroatSWtgeteirS)coelyiuStoiolnw"{1S0H0I0RiAE4AElL a BBRogEog)rereFs1.o0i00creos:1581 algAas.u CuCleoRrininnidtsisrfoal BQ 25550 SCO O L pikE ea RGSTAfin AE fou Tae thes] 2E | SHE ZFoLlAi i8CoopTeesrtatLontng oafndCDehveelompreinotrcSgaaancilviaSsstPirs:na)nCoern Eonsn (=2)i.s1o5/0o1i9s878.6p5a2t. eirnQtuearlnaitt(ysshahliigoerisfaoseidohneiniEiinAtnsiblETo c{lcud d 8 5 Son BE R IEE D|cR an avio tierv ande Faia dae ox: 4 > REL0I BA a E ; E ii ASAa E i a Ae 001101 ENVIRONMENTAL LABORATORY sFaAfSe E STANDARD NUTRIENT MEDIUW FOR FRESHWATER ALGAE >,ry orRzEnT InCoSTnOcCaKntSuOrLiUoTnION |pIuNnTsEScToSvOcLevUTTaItOiNon STOCK SOLUTION 1: WACRONUTRIENTS wHnobcll,.6h,0 cac13:2H20 11..52 gg//LL 1a 5 111582 mnmggo///LLL Haoes0d2 ,70 s1sieg/nt 15Lmeoshtn stock sounion 3: raeoma ] roc 6,0 wag 80a @ Na ,EBTA. 31,0 100 mg/L 100 ug/L Q STOCK SOLUTION 3: TRACE ELEMENTS o 1TA,nc8e0a,) an,0 411855HE nmgg//LL 148155wuugg//LLk2 CcouCc1122.:62Hn,200 01..051mng/gL/L w1.5aug/iL sZB Na M80, 30 7 mg/L 7 ug/L zc STOCK SOLUTION 4: NaHCO, @ S(tmeerainlipzoeresdtiocakmestoelrut0i.o2nsum)1.-4 by moet mbrane filtration ( SOLUP TIONr f5r:ase NnUlTyRp IEbNeTfoarCeONCr eaEcNhTReAtTeEst.) Add 1011000 mmALLL ooff of SSTTOOCCKK STOCK SSOOLLUUTTIIOONN SOLUTION 21;1.1: ii 3; iAsifigis and 10 at of stock soLution4. GHEE. ' Mrakaesuap nto o1i,00t0o a3.L3"wit8h Tap|} eQBS. ater. i I SOLUTION 6: STANDARD ALGAL NUTRIENT'MEDIUMECZOSCE Sock 1000u[lqL Atdod_910000anLLoOff Milthe pi-adjusA ted NUG PHLO Eient CA oncentraatteer.Solutiion Ea eT LS i WV R(E1)FEREONECCEDS:19843 Alga, Gr3owth foireating ofchemicals IonrhiigbaintliosnayTteisot3n4201aiiSnetipcGonuonlie.de|loinlesaAi(EufBiDis GT Cosooper10a0t7i.onwataenrd DoeuveelelyopSmaenitgsPe}arGoin TiaPrahnceis niioni Test 0 a A i Lana ed aa ie fs aGtU: , 150/D1S GEeEnTeve, S8w6i92t.ierIinetnaedri.nliatimonealgfOrgapizaBtEiEoL nifor n StandarEdRizaetison iesSRE SEI SRE R GRATEE + Lnhe hRe RAE ah a 001102 FRESIMABTEeR ALGAE CRONTH-- TaBTTIGH TEST ALGAL CELL COUNT, No./slL HEMOCYTQMETER METHOD* wil[pu ) WEILTST LL 2DBCE mstvomAST Lnfor) ) ser sig ALGAL SPECIES: Green Algae, Selensstrim capricormatun wy weas/r/sg ANALYST: Zr Labuan me Toma oF25 pres y . ey Fe205ceT| on Vga Io 21-72] ecR2E31 1-4 00,4 270 aw asy 200 22&352 U5 7 yg 2, 700,000 PL 2,550,0)00 2,406, 000 A82S0,e0e0n0 74)4054002000 776,000 150, 000 m9 8o >2 Z=< gs . @ aS . C aShniaa a - I EE ET vbooolT laacs Alls TestTepenatin 25/ (AEEERIE TEA @ *TeE st0-1mmemtdheoetpolHS iN geycG moE notecstysE R CthaioR rnN ena ag osfftfaioelAlrESda1sox o1 3anwdes0.1provstrmomtdeoC ecp.ola sE GRpAilSepidR er BLT a RBA T oo Smisiasatath oe SE ls CEE ie a RE SRT Re cottiiin : 3017001103 re--name rrm-- s TT ----T-- E Ln mni (ZUe RS, H. wr 2805 7. fa) deresz iF Q...... he ierig AGL SPEciEs: Na po So Tr a Environmental Lab ~WorkSheet (COMPARISONOFGROWTH RATES rola ne 2 coir Gn ls Sslmton oncom rc TREATMENT 4 -DAY. v " oy 4] -DAY Thes =n s ov[3 aa388ySTE; @ 2 Zap - Cush 1% pl | psy Le LST 2310 9 Q 5 eee ccoughl-4 x05 1.25349" Pe eR g > Tas ennST ogg s -_-- -_-- 000 @ 000 i )Specific Gronh hate Bo + nM laWgi hay on pe ma FE a SERS Sages se Ry a agHE HERERESTIR Ns Sa zy ei i Ee ; 001104 EWIROMDITAL LAsorAToRY FRESHNATER ALGAE GROWTH INWIBITION TEST AALGAL CELL COUNT, No./ml. HEMOCYTOMETER METHOD* @ nor po TEST TYP : MAALTGEARLIASPLECTIEESST:ED:Green2%Algae, Selenastrun 2capri2cormtin 57 AWS Zab, -- ee ots wre SL/R [BK CowrcT It TIT Fe eace, 225580 255 22,,S5H1C8,,000000 | 2,$559,00, 1COm=ah_t1A- 1 ZI1s0 Vceio, 115043 22,116x, cCeoet 1)VeSrhcei, ecceo Sreod3 s %Te S 5 O1,-41 E3Z0 esCeC SeSacge0c0o0 Cowe d1aS 2Z4o | 23400,0%00 ScRhEEmRER 1 EE - : Soa as pai.3 wmo as = 8 eotd 5 Ed: =>< ke. E>g [ZX [7-3 : spdtilaneg HR Lot teersRACERS "0-1 despHemocytemeter; arealoFisield 1 X1and 0.1mm deep. EINE ican E zLoT8e1a3cGUmSe-EpEhPoA2Dd0#o1LElCo-"n8pNy, "gcAaool;ngfcaoldrraAsceuuwtiIetnmhTvoitxbhmielclaitttaaoyn,lBlmTeiesnstegt;tShspoA!Ei)teosT nt protoE colC s: B4.E1: 1n 1.14enER i Spmghe SSE E aae ad C rR m Y n ; p enwe. 00331053. 05 : ob Requestho.ZLBST=/ Environmental Lab -- Work Sheet o--at 7e `SampleDescription ALGCAOLMPAASRSIASYO:N BOOFTTCLREOTWESTRTs:BT) [SRv2). R ma y -- waren este:A205 CF ALGAL SPECIES: (2/21/89) dor 519 poor --F 2 Selenastrun capricornutim TREADENT AY T count wy AY 13358 < lL = - xl St I=. v - 220), Slaugh 000g), . 2% 12. 1S 2S xjpl 4 105 20 X10 58 4105 SpA 204 X405 lo 10S ai ma -2 0455 ols 029-24 062 DS 20.3045 --iz 0.845 ------------------------------------------ --i---------------------------------------------- -- err------------ eee-- S-- --se -- --(1) Si pecifeic Gerowte h Ra, te ( = od ln --,,,,---- ----(2) DescentCuange inGrowthBate (Consol Semple) oo Control ------eeieS-------------------------------------------------- c-- e ------------------------------r-- eAeV--AeIL--AB--LE -- tr--e--e--s c--e ---------------------------------------------------- 001106