Document 2qVM9bxOL4oDDBo09Rzpo0DOg
AR R26 -- 0 805
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9i5u,oroorocatsanaescuolmfponoanteen;tmoafyFaCl-s2o0b6eC a1,n1d,28,12-,23/,83.,4,(41,-5O,c5,t6a,n6e,s7u,l7f,o8,n8ic8-ahceidp,tadecafiuoro-, potassium salt, CAS # 2795-39-3)
Riseamamrikxtsu:re ofT0h.e7t3e%stPsFaOmSp,le76is.F0C9-%20w6aCte.r,C1ur5r%endtieitnhfyolremnaetigolnycionldibcuatytles it eptohleyro,x4ye%thuyrleean,e1m.o5n5o%ocstoydlipuhmenoycltyelthsuelrf,at0e.,016.%4s7o%dhiyudmrloaxuyryflosaumlefra,te,1.a0n5d% 0.05% benzotriazole.
The following summary applies to a mixture with incompletely rcehfalreacctttehreizteodxiccoitnyceonfttrhaetifolnuoofroicmhpeurmiitciaels.coDmaptnaemnatoyfntohteactcesutrately
sample.
METHOD:
Method: USEPA - 600/9-78-018 and ASTM-E-35.21 Draft No. 1
TGyLpPe: (Nteost type): Acute Static
Year study performed: 1981
Species: __ Selenastrum capricomutum
Smaoiunrtcaei:neOdriingicnualltluyroebmteaidnieudmfratomthUeS3EMPAE-nEvRirLo,nmCeornvtaallliLsa,bOorraetgoorny,, St.
Paul, MN.
Element basis: Exposure period:
A9l6gahloucerlsl counts (celis/mi), cell dry weights.
Statistical Methods: Regression model.
Analytical monitoring: Not given.
Test Conditions:
A"TlhgiaslnuNturtireintenmteMdeiduimupmr:oviNudterdiealnltmmienedriaulmnupterrieUn.tSs.eEssPeAnti6a0l0/f9or-7a8l-ga0l18. tghrioswstyhntahnedtiaclaslogaslermveeddiausmthweasdialdujenutstfeodr atlol 7a.lg5aplriooprertaotuiosnes.in TashseaypsH.of
tShteocalkgaalnsdttaensdtarsdoltuetstiomnedpireupma,rahtoiwoenv:erStnoocfkusrotlheurtidoensscrwieprteiopnrweapasred in provided.
001686
Exposure vessels: 250 ml Erlenmeyers containing 50 mi test solution.
Agitation: Shaken continuously at 100 pm. Number of replicates: 3
Initial cell loading: 1.0 x 10 cells/mL.
NLiugmhtbienrgo:f40c0onfcoeontt-rcaantdiloenssf:rfoimvecpolnutsinauboluasnckocooln-twrhoilte fluorescent
lighting.
Water pChHemriasntgrey(:0-96 hours): Not noted. Test temperature range (0-96 hours): 23 + 2C (incubator)
RESULTS
Nominal moll
concentrations:
Bk
control,
280,
420,
650,
1,000,
and
1,550
Elemen9t6-vhaoluuresE:C50 Cell-Dry weight: 345 (3--4 1,630) mg/L 96-hour EC50 Algal Cell Count: 379 (106 -- 1,137) mg/L.
Element values are based on nominal concentrations. tReesvtersosliubtiilointsy woefrGerdoiwltuthedInwhiitbhitailoganl: mAleidqiuoutmsoafntdhceul1t,u0r0e0d afonrd110,d5a5y0sm.g/L Based on growth observed in the recovery phase, the effect on algal
growth was found to be algistatic.
Remarks: Testing was conducted on the mixture as described in the test
substance remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
(CLUSION: The test substance exhibits a 96-hour ECs cell count value of 379 (106 -- 11,,163370)) mmgg//LL.and a 96-hour ECs; based on cell dry weight of 345 (34
This test substance was determined to be algistatic.
Submitter: 3M Company, St. Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
DATA QUALITY
001687
Reliability: Kiimisch ranking = 2. This study meets the criteria forquality testing. However, the study lacks information on purityofthe test substance andit lacks analytical confirmationofthe amount of fiuorochemical proportion in the solution.
REFERENCES
Testwas conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6812S, 1981.
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Last changed: 6/26/00
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TECHNICAL REPORT SUMMARY
23/01
470: FIEdCARICAL COMMUNICATIONS CENTER = 201.208
impoSrtEant Ifraport isprintedonbotsiodfpaepesr, snd tw copi0e TCBC.)EST COPY AVAILABLE
Environmental Engineering and Pollution Control (Envir. Lab) | ocoo"
Seite Corre. over, wv
Algal Assay: Bottle Test (AA:BT), Toxicity Testing FC-206C
9970012600
|oot
E. A. Reiner - 21-2
LR 6812 5
M. T. Elnabarawy - 2-38
e081
Scumrvp = Bom BwerewoonnoSssr:raomm aonmer
oPasin me
EE & PC Div.
(Env. Lab) +
o%ows
SoREmeA
on5ow rGormomit gre
CURRENT ORRCTIVE:
;
"`o0dlaldyoewteeedrxmpbioynseuraeth1ep0e-draailyogadlrteocgorvvoeawrtryhyirnpegesrpicooondn.sceesnt(rEaCtgl,o,f.i)s offolFlACo-w2i0n6gC,a
Aer Bottle
Tost it y
ONT ABSTRACT (2050 waa Tos ns on ond Tem CommoGno ad
he algal acute 4-day ECg, values obtained for freshwater
[lgErCe-e3n06Calgianet(rSIepleTmiacstartuem@aprexpiercimoenrtsnuwteurme) 34f5ollaonwdin0g70emxgp/o1s:ure [[bTyhecseellv-adlureysweriegphrteseanntd athlegalalgcaelll-gcroouwntth, rersepsopnecsteivaeslym.easure [lNeovetle.st ma-t-erialconcentration was defined as the no-effect [rheecoavlegraylpebriiomoadssinmdoinciattoedrinignsmiegansiufriecmaennttsalgfioclildoawlingeffaec1t0-adta [acnodncreensturnaetdiolnosgatreistthemdi.c gSrionwcteh wthheenalrgeasluspceenlldsedhainvefrreeschovers I2nsedainun,inthhiebi4t-ordyayemffeedcita.n growth response (ECgy) is interpre
innfeormation sien
Thal
SESERESRR ERfe R RT EL R 001689C."E
FPaCg-e206TwCo/MTE June 23, 1981
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CONCLUSIONS vIan lutehsesefortrifprleischawtaetder 4g-draeyenECaslggaealg(aSlelaesnsaasytsr,umthCeapcrailccorunluattuemd) vreersepon3s4e5aansdme3a79sumrge/d1.by cTheelsle-vdaryluweesirgehptreasnedntalgalthecealllgalcougnrto,wth respectively. Nloevetle.st material concentration was defined as the no-effect Trheecovaelrgyalpebriioomdassinmdoincaitteodrinlgittmleeasuorremneontaslgifcoildlaolwinegffeact1s0-adtay . rceosnucmeendtraltoigoanrsithtmeisctedg.rowtShinwcheentheresaulsgpalendceedllsinhafvreeshremceodvieurme,d tahned i4n-hdiaybitmoerdyianeffgercotw.th response (ECs) is interpreted as an
INTRODUCTION aInquatetsitcinegnvithreonmpeonsts,iblueniecfeflelcutlsarofalgcaheemiacrael rseucbosmtmaenncdeesd oans tahemodel psryismtaermyfoprrodeuvcaelrusa,tinpglantthse, iannfdluepnhcyetopoflanckhteomni.cals on aquatic Tcoexlilc-diefvfiescitosn adruerintgesttehde otnestg.rowiAnlggalalggarlowtchultruersepsonstehatinuclnuddeer:goa) naolgiecfifdeaclt., b) stimulatory,.c) inhibitory, d) algistatic, or Tahmebietnetstrosoumbsttaenmcpeeraitsurae.cleaTrhesaembebrioalsisqauiyds,pesroflourbmleed ionnwatthiesr at mgaetneerriaatledevfaolrmuattehde biatssispootfenttihails ralegpaolrt.toxicity and the data
MATERIAL AND EXPERIMENTAL DESIGN Tbhyesae 1a0l-gdaaly arsescaoyvseryconpseirsitode.d ofThea t4e-sdtayperxoptooscuorle upteirliiozded fofolrlowtehdis santdudyASTvaMs-Em-o3d5i.f2i1edDraafftterNo.tho1s.e d(e1s)c,ri(b2e)d. by USEPA - 600/9-78-018 Test Species:
ASecluelntausrterumof Cathpericforrensuhtwautmerof gtrheeenoradlegrae ch(lCohrloocroocpchaylceesae)w,as SThhettaelstnefadrlogaUme SareHn=EoEn-RRmEo,tilCeorvu.n,iceOlrleugloanr (cPeelblrsaaraynd 1h3.avi1n9g81). itnhethaeppdeaarrkancaet o4f0Ca nunetwilmoouns.e. The algal culture was stored
001695
FPacg-e20T6ncr/eMeTE
June 23, 1981
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Inoculum:
:
gAilvgeaea fsrotmareain7g-daoyptiomldumstioncokculcuumltulreevelwerofe gurseedenasalgianeoculum to
Selenastrum Capricornutum 1 x 104 cells/ml. The use of a
7-10 day old stock culture will insure a sufficient amount of
viable algal cells in the exponential growth phase. The
. initial algal cell count in the stock culture was determined
with the aid of a hemocytometer (370,000 cells/ml).
Algal Culture Mediums
Standard culture and test medium vas prepared as outlined in
Attachment #l. This algal culture medium provided all
"
nutrients essential for algal growth and also served as the
diluent for all algal operations including the preparation of
stock solutions. The pH of this synthetic algal medium was
adjusted to 7.5 prior to use in assays.
Culture Flasks:
Bach 250 ml erlenmeyer containing 50 ml of test solution
. comprised a test flask. Culture flasks and all other
glassware were specially prepared as described in Attachment
#2. Autoclaved foam plugs were used as flask closures,
allowing for free gas exchange.
Range-Finding Test (Exploratory):
bThieomaesxsploinrastionrgyletesftlasckosnsicsotnetdainoifngdetsetranmdianridng cutlhetur4e-dmaeydiaulmgal
plus the test material at concentrations covering several
Jders of magnitude: 0.1, 1.0, 10, 100 and 1,000 mg/l.
Full-Scale Test (Definitive):
AuIsTingdef2i5n0itmliveerlalegnamleyearssafylsaskwsereconctarariineidngou5t0 miln otfriptelsitcate
(subculture) stage provided information on solution. The definitive test consisted of two culture
stages: a 4-day exposure period followed by a 10-day recovery period. The purpose of the exposure (contact) period was to verify inhibitory effects. The recovery
algistatic/algicidal effects.
The following FC-206C logarithmic concentration: 2800 420, 650, 1,000 and 1,550 mg/l were used to initiate the 4-day contact period. This dose range of dilutions was selected to
bracket 4-dayECs0 values predicted from preliminary -- -- exploratory testing. Three flasks containing 100% fresh
algal culture medium plus algal cells comprised the nontreated controls. The recovery stage was initiated by
be? A aERs HLS Gores oir
FC-206C/MTE JPuangee 2F3o,ur 1981
BEST COPY AVAILABLE
sofubctuheltutrwionghig(h0e.s5tm1t/e5s0t mclo)ncefnrtormateiaocnhsofinttohefltahsrkese croenptlaiicnaitnegs fresh algal medium. These two treatments induced inhibitory effects greatr than 80% during the contact period.
tFhreeshalgsatlockstasnodlaurtdiontsestofmetdhieumt.estSeseubsAttatnaccehmewnetre #3p.repared in
Test Conditions:
cAlolndiatligoanls,opeinraotridoenrs twoereavociadrriceodntaomuitnautnidoenr wsittehrilbeacteria and oentvhierronamlegnate.al Aclhgaamlbercuulntduerresthweerefolilnocwuibnagtedstainndaarnd growth conditions:
.
*Temperature - 23 + 20C (70-779F)
*Fluorescent illumination - 400 t.C. + 10%
*SFrheaekinggas10e0xc+han1g0erp-m.Continuous Platform -
Algal Biomass Monitoring:
wTheeighatlga(lmg/glr)owatnhd rbeyspocnelsle wcaosuntmea(scuerlelds/mbly).algaBlothceglrlo-wdtrhy mexepaossuurreemenptesriowdereandmadoenlywitchella-lcgoaluntcuwlatsuremsadeafwtietrhtthhee 4-day osfulcbuilotmuasrsesmeaafstuerremaen1t0s-daaryerbercioevfelryy dpeesrcirodi.bedBoitnhAptrtaocchemdeunrtes 4.
EutCislgizvailnuges.3anSdIXC9U5R%, coanfTiRAdCencSeystleimmitfsorwerreegrecsaslicounlatmeoddels of
experimental data.
--
TEST RESULTS
TSheelenraessturlutms oCfaprbiiocmoarsnsutumemasuusreedmeinntsthoefsetshteudgireesenaraelgae SreesuTlfts-eofxpthle adneafanidtnaiotrirevyedteetsatileadreinsudmamatraizsehdeetisn Taatbtlaeche1d.. The
ae
Ret
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Feg-elpsle E June 23, 1981
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Table 1
:
Definitive Test - ECsgprviaclourensu,tumng/l with BSealesnaastlrutm
TTType Test
Cell-Dry WeighAtigmagl/lBiomassCellWoCnolutnortinNgo./1 ml
4-DayPerEixopdosure
345(34-1630)%
37910651137)
+954 Confidence limits.
- TEhlenabdaartaawyr.epoArctceodmphaenryeiinngadreatabassehdeetosn csotmupdriiesse poerrifgoirnmaeld dbaytaM.. T.A Acorpcyhivoef Styhsitsemr,epoBrutildiisngfil2e-d3E.in the Environmental Laboratory oInt tshheoulbdasibse onfotetdhe tchaatlcutlhaeterdepoirntietdialECcsgoncveanltureastiwoenrse oefvalFuCa-t2e0d6C in test solutions. REFERENCES (1) MSiellleenra,stWr.umE.C,apJr.icCo.rnuGtreuemneP,rinatnzd TA.lgaSlhirAosysaamya.Bott1l9e78.Te-stT:he UP.ES.TEInevritraolnmHeentalonePraoptbeTcitciaotnioAng,encayn,d dCaotravalilnitse,rprOerteagtoni.on:
EPA-600/9-78-018. 125 p.
(2) CAoSnTdMu-cEt-i3n5g.21T:oxic1i9t8y0. TesPtrsopowsietdh SFtraensdhawratderPraancdticSealtFowrater Algae. Draft No. l.
:
~~
EE
La
mai
0016934
:
type Test
ALGALTOEAXNSIVSCIAIRYTO:YNMBETEONSTTTABLLEELTASEBSOTTR5AOT(OAPRAY:Y8T)AvaILABEE-S-L2.2-5
Qay Faposose
Material Tested FC -206 C tor physical Description Clear Auber Liquid
Algal Species GE reen Algae, Selenasst ttrumnccapar prilcooronmuetnunm
source Cac za\ls Ocegon Date Rec'd 2-18: 81 Age of tnoculun__Tday
Test Container:250 m Erlenmeyer flasks with foam plugs
Sample-to-volune Ratio: TO to 20 initial Coll Loading: 10000 cells/ml
Initial pH of Synthetic Algal Medium: 7.520.1
Test Conditions: Temperature =2322% Zey
.
FFlrueoereGsacsenEtxch|a1nTguemin=attioonn = 400 pft lta.tcfo.r$m1-0t%shaking at 100210 rpm.
Date Started___G = I~ Bl Date Completed_G = 1S - Z| Investigator MT E lng. bar
ALGAL CELL DRY WEIGHT, MEAN VALUE In ma/i (1)
TYPE TE
TREATMENT
EXPOSURE: 4-DAY CONTACT PERIOD
[RECOVER:Y 10-DAY SUBCULTURE PER10D
CONTROL
J
20
5.0
--_--
m Ar_ tse LLe oBn
000 erated
%
Ls
or 206
re.nin QR nsem----------------
----------------------------
emend
gon
9
--
seven
ONTRO
280
RIAL YET,COUNT,SMeEATNeVgALUL No,/m (1)
oooone von comerpeng
00,00
Meccan: jon
he si
2 40, 000
_
2.000.
200
120,020
--Looo2
een000,.000000...
emnlS0 O00Q0 00
A
g
--
RESET 1 creer age | SGSIALCALGILD
(1) based on mean values of each triplicate sets offlasks.
(2) Method of calculations;
BEST COPY AVAILABLE ?
00169.4
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ALGAALLGACLERELECLANOSVVSCIEARRoYYO:rNM,EBSNOTTTCAATeGLLErEiaDL/ATAeTEBtASOTRAS(Tun(ORAeRtAaYA:RBST)RST
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com| o | qe|
\ wo |doo|
ro = YS
I EE
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Fr
240 0m cone
.
(1). Based on 0.5m1/50m1 subculture made from b-day exposure cultures.
(2). 10-day recovery cultures.
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