Document 2qGxmYJE9dJR9ODoXmG47MV0L
AH SUL 6 -
ACUTE TO XIC ITY TO FISH (FATHEAD M INNOW )
TEST SUBSTANCE________________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC -3031, (1Octanesulfonic add) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 2. The test sample is FC3031 . Current information indicates it is a mixture of 0.96% PFOS, 20% diethylene glycol butyl ether, 62.11% water, 10% ethylene glycol, 1.92% propane sultone foamer, 2.02% polyoxyethylene monooctylphenyl ether, 2.88% sodium octyl sulfate, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.
The following is an abbreviated summary o f multiple fathead minnow studies on FC-3031, Lot 2. These studies apply to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:________________ ________ _______________________________
M ethod: Not given.
Type: Acute static
GLP: No
Y ear com pleted: 1976
Species: Pimephales promelas
Supplier: Not given.
A nalytical m onitoring: Not given.
Exposure period: 96-hours
Statistical m ethods: LC50 values determined by graphic interpolation.
T est fish age: Juveniles
Length and weight:
Average length = 4 8 - 5 0 mm
Average weight = 1.4 -1 .5 g (wet)
Loading: Not given.
Pretreatm ent: None.
T est conditions:
Dilution w ater: Aerated carbon filtered well water
Dilution w ater chem istry: Not given.
Lighting: Not given.
Stock and te st solution preparation: Not given.
C oncentrations dosing rate: Once
S tability o f the te st chem ical solutions: Not noted.
Exposure vessels: Not given
Num ber o f replicates: 1
Num ber o f fish per replicate: 20 per test solution for one study. Not given
for remaining studies.
002860
Num ber o f concentrations: five plus a blank control W ater chem istry ranges during the studies:
pH range (0-96 hours): 7.2 - 7.4 (control exposures)
Tem perature range (0-96 hours): 21 - 22 C (control exposures)
Dissolved oxygen range (0-96 hours): 5.0 - 9.2 mg/L (control exposures)
RESULTS_________________________________________________________
Nom inal concentrations: Blank control, 100, 250, 500, 750, and 1000 mg/L for two studies (7/6-10/76, 7/27-31/76). Blank control, 250, 500, 75 0,1 0 0 0 , and 1250 mg/L for one study (7/20-24/76). Blank control, 100, 180, 320, 560, and 1000 mg/L for one study (8/10-14/76).
Elem ent values: 96-hour LCso range = 560 - 800 mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS____________________________________________ _
The test sample 96-hour LC50 range for fathead minnow was determined to be 560 to 800 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
R eliability: Klimisch ranking 3. Testing lacks record of methodology used. There is a lack of characterization of the test sample. Test solutions lack analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________ _
These studies were conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1976.
OTHER_________________________________ ;_________________________,
Last changed: 6/26/00
002861
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test 96 Hours --
Material Tested
Test Organism Fathead Minnow
Avg. Weight
Date Started_______ ? / / 7 6
Date Completed
Time Started________ OZOOpfr).
P lotted LC5q__
D ilution Water Carbon F iltered - Well Water
Jfe
Avg. s i* e_ 4 . - ? r>vs,,.
ih o /7
~Jna m
Cone.
Su2r4v%ihvarsl
pH
D.O JE
In
Control /as
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Su7r2v%ihvrasl pH pDp.mO Su9r6v%ihvrasl PH, -Dpp.m
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ACovmermaegnetsT: emperature
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S lo
[C
40 7.0 4 , 2
2.1
Z o 4. 2L
o . Ip m
21
% Survival
S
002862
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
TyPe T est____ 96 Hours - Q f t f / T . Test Organism Fathead Minnow
F -3 OSlcMaterial Tested
Avg. Weight
. \.^
Avg. Size
QlV
Date Started______ ~7/'Zo / "7h
Date Completed_______ 7 / 2 4 / 7 6
Time Started
a ! 2 n m .
Plotted LC50-
J f2 .c l m d )1
Dilution Water Carbon Filtered - Well Water
Cone. Control
24 hrs %
D.O
ioo i h 5.1
350&W LAoo (7 f
7A
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ISq
ioo 7,0 5.2.
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l o o 7,0 5.G
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7,o 5.0
Average Temperature \ C i
48 hrs
D.O
too l A S f c
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6 5 Zo 4,2
O 45
0 6 4 3
72 hrs %
96 hrs D.O %
D.O
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loo Z *
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Z o 4.1 4.1
loo 0
Zo
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______ 7 X \
7
LCgQ Calculation
EmD
33o
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AVAILABLE
40 50 60 % Survival
100
00286S '
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
TTM e Test____96 Hours - Sj-flHf. Test Organism Fathead Minnow
( ,DMe S' " " 1_________ 1 127 i f
3frooph/vTime Started
W Material Tested_____ Avg. Weight
-3 s > t
Avg. Size_ S
P.Wa .
Date Completed
iL u U .
Plotted LC
Dilution Water Carbon Filtered - Well Water -C fS e E t e Q
Control
24 hrs %
D.O
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loo
74 U .
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loo loo
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48 hrs %
72 hrs
D.O %
D.O
ouivivai rn ppm
Io 1 3 6,12. 1(70 I x r . s
96 hrs % D.O
survival _LJ2EL
[oo s~r
loc? 7 2 a
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7.0 C o 0 7 o r.C-
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________2 1 _______ ______ a t
i
LCgQ Calculation
40 50 60 % Survival
CD CmO
H O O "U <
CED
100 Im--
002864
740101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test 96 Hours -- Test Organism Fathead Minnow
Material Test Avg
Avg. Weight __ l S i . Size
Date Started_________$ / / < ? / 7 ^
Time Started
IXzoQp tn ,
Date Completed Plotted LC50-
>P,o
Dilution Water Carbon Filtered - Well Water
d
24 hrs
48 hrs
72 hrs
96 hrs
% D.O %
D.O %
D.O %
D.O
Cone. Survival pH ppm Control
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Survival pH ppm
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Survival pH ppm Survival pH ppm
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Comments
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CD
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% Survival
00865
