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AR 226-0HoH BIODEGRADATION TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, N- ethyl-N-[(heptadecafluorooctyl)sulfonyl}-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C7 fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg. date 3/97. The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data does not accurately relate biodegradation of the test sample with that of the test substance. METHOD: Method: Not noted. Type: Aerobic GLP: No Year completed: 1997 Contact time: 28 days Inoculum: Polyseed NX, lot D257 RESULTS COD: 462,000 mg Oz/ kg chemical BOD First Replicate BOD Second Replicate BOD [5 <8 | oso | [FoT18 | 6 sers0 0 | [20 T"&70 | 7e400 | C2 1 289000[280000| Remarks: The BOD and COD values are expected tobethe result of biodegradationof the organic solvent componentsofthe test sample. These values are not expected to be representative of the Perfluorooctanesulfonylamido(ethyl)acetate. 000353 Co CONCLUSIONS This testing indicated that some portion of the test sample biodegraded in aerobic environments capable of supporting biodegradation. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. This study lacks information on the methodology. Testing cannot conclude what portions of the test sample were responsible for the apparent biodegradation values. Comparison `with the COD value for determination of the level of biodegradation is not valuable without a description of how the COD value was obtained. REFERENCES This study was conducted at AScl Corporation of Duluth, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00 000033554 ce SuEbAnmSiietrleodnCnotmoopt3oaMrltTConoe/miApnSagcntyDDi-vui1s9ho7n ASCLDETD Study ID 45010.068 R1904 BOD REPORT FOR 3M COMPANY Submitted to 3M Environmental Technology and 935 Bush Avenue Safety Services Building 2-3E-09 PO Box 33331 St. Paul, MN 55133-3331 Prepared by EAnSvcilroCnomrepnotraaltiToens/tAiSncgl-DDiuvilsuitohn Du4l4u4th4, AMiirnpnaerksoBtoaule5v5a8r1d1 SSppoonnssoorr. Su3dMyCIoDmWpRa1ny04 JuLy 1997 . 000355 COD = 462,000 mg O, / kg chemical. Dates of BOD test: 6/11/97 - 7/9/97. `Water bath temperature range during the test: Lot number of Polyseed NX: D257. 19.0 - 20.0C. SuEbSmmcirttoedCnomtmsepMnoaraCTiooonnAgoSenDDotonys SIDED Soy 5 Poses Pr C lal TABLE 1. BOD Dilution Information for R1904 | pen | cmmmimen | Tw | em o se T 0] L ws |T es [0 [ s [eswo ee T | wo as T | w an | TABLE 2. BOD Blank Calculations for R1904 1 _ |ET FIRST T REPLICATE _ TsT cowmemomeJ | * Jano [reino |ooom |wino |rmino | 00ow| [owTs[ wo | ow [ a0 | ou | ow |o | [ow [oo[a0 [on[ow |i 0|an |on| Soma sy 53R104 : -1- 000356 bEJmergCorponftoounnsDtcenkn Se eo TABLE 3. BOD Seed Correction Factor Calculations for R1904 [== HEBEEERERREEl] -- - [oreo] |I seed onecone. | Day f wou | oo cowEmmTon |] ron| oo. 1 woAverage To [onTow[on onTeaTonToon] [ L0 o T[oolboow[[5a0e[ To0n[Tooaw |oonnT[eow [|o2ns0[|aonn ||oamw|| *SCF = (D.O. drop *mlseedin test) / mlseed inseedblank. TABLE 4. BOD Reference? and Tox Blanks Calculations for R1904 = To ae[on [oo oe i[oT | [ oT Conc.| Day| SCF rem | e oowmmm on I Mfeo [5[on]RosooTew T1oDnp[|cDoroT|p omyth[|eDwO[|oDOa.[| oDrnop[|oDerT|o omampt.] (oa [JooLoo [an[onTosToon [rus [na[sm|vs|] * Reference = 150 mg glucose and 150 mg glutamic acid in 1 liter BOD dilution water, tested at 2% dilution. BOD mg/L = (corrected D.O. drop) * 300 ml BOD volume / 6 ml reference. Sr . 000357 SEmAsetrgCoipoTCniosnnigtoron5tsnetn pr TABLE 5. BOD Calculations for R1904 |Iccooee| ow FEE |ser |mFe ou| Do. r | come|o 500 HEEEGE cc [ Fo ou|w 00|mcornes|o50n 0| [[oieoesTLsssTTToooooalPoaonwwT[Poooomae[[FuoonneT[[oovsaoe| T|o=] m[oi oom[[e room[Luon aoe|[rooo snwe| T[on 7o--n]|| [a ee [rorToTow | as Tem[oe 1 [20Jovomo|I wou[7a[ame| am|=] Fue ToTow ow Tomo[vo [am| oro [om[am| aur|ome | pei [ioToco [eoLoo Too [asoTame[ore[ss [mmm [ooo|ase|536| are | woo | Le TomTos TomTomo[asoTous[as|ows| -| 1 [verse] [osTooToo J| as ToonTow[om | 1 = [oo[ow[10 | am | | Live [0Loon [21[20Tow L| aoww T[osmo[{2arm[[uosme [|nn|[oomw|[eoosn[[a7se || oo| - | aw |sem | [5L 00[anToe m | om[wam n ws|5] s[wo| om [ass|535 |soo |somo| rr [io TosTroe|aso [ase[soe |26[osamo | oor[se[ws|a | emo| [isTos[vos[om [ow[ow[sur[oem | so [225|oes | 557 | sono| PaarlfeeLealonLom] eww] ew] * BOD = corrected drop x 1,000,000 /test concentration. Omidtatatpoe intds: FiDn.aOl. Ddr.o0p. c<or0r.e5ct0edmgf/oLr.SCF too low (<2.0). Som Sty mais : -3- 000358 EAnSveilroCnomernptoarltoTne/vAiSncgl-DDivuilsuitohn SAuSbmCiDteEdT1D 3StMudCyoImDp#a5n0-1y0.705678 APPENDIX A -- Raw Data Sheets SpSopnonssoorrS:tu3dMyICDom#pRaInSy04 . 000359 BIODEGRADATION (METHYLENE BLUE ACTIVE SUBSTANCE) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be FreCf-e1r2r7ed, tFoC-a1s2P9,FOFSCA-A10,9,FCF-C1-21809or-XasorthRe19m0a4j.or (cGolmycpionne,enNt- of ethyk-N-{(heptadecafiuorooctyl)sulfonyil-, 2991-51-7) potassium salt, CAS # Remarks: The test sample is FC-129 referred to by the test laboratory iansfosrammaptlioenGi1n2d0i8ca-t1esaint disraepmliixctatuereaonfal4ys2i%s atessstasmupbslteanGc1e2,083-42.% Cwuarterre,nt 14% 2-butoxyethanol, 4% ethanol, and 8% Cs-C; fluoroalkyl carboxylate compounds. Test material was from 3M production lot 564. The following summary is abbreviated because thetestsample is a mixtureofthe test substance in a water/organic solvent solution. Data does not accurately relate biodegradation of the test sample with that of the test substance. METHOD: Method: EPA Method 425.1 Type: Methylene Blue Active Substance GLP: No Year completed: 1989 RESULTS G1208-1: 2200 mgikg G1208-4: 2800 mg/kg (duplicate of G1208-1) Duplicate EPA LAS Reference samples: 60,000 mg/kg 60,000 mg/kg CONCLUSIONS This testing indicated MBAS study. that the test sample exhibited minimal activity in this Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY 000360 Reliability: Klimisch ranking 2. This study lacks information on the methodology and testing parameters. Test substance purity within the test sample was not sufficiently characterized REFERENCES This study was conducted at Pace Analytical of Minneapolis, Minnesota at the requestof the 3M Company. OTHER Last changed: 5/17/00 000361 ee ". 0> 2/22/1989 ENVIRONMENkTtALRELVAIBSOERDATO%R4Yk FINAL REPORT 1 LAB REQUEST NO. G1208 : RDEEQPUARETSHTEONRT:NAM0E5:35EA REINER PDARTOEJECRETCENIO:VED9:970020/10031/010989 DESC: MBAS OF FC-129, TRITON HG CCOONNTTRRAACCTT LLAABB: COPSATC:E 162 E3XKPB-COLMPHOUDRAST:E: 020/17/1989 DATE COMPLETED: 02/15/1989 SSATHAPNLDEASRDSLHAOSULDSOLBEUTIDOENLIVFEORREDCOMIPNARWIYSONNA.ME TDOO TAHLEL LEAXBT.RACTRIUONNSA AMNEDASUBRAECMKENVTASHONCTAHLELED FSOTRANIDNARDPROICSEDPURROEB.ABLEFC-A1B2O9UTIS3255.0% TSEOSLITDSF.C-12I9TSATMVAIMSOL62A3R.COTNHCEENTMRVATOIFONTHEEQULAALS TO TGH1A2T08-O3F LIASS.AN EPA LAS REFERENCE MATERIAL: LOT $1138, AVE. M.V. 318, ACTIVE LDAESTE6R.G0E0NTX,ASSPORCEIPAATRIEODN,BYDAETWESL9C65I.NCINNATI US EPA IN COOPERATION VITH SOAP AND Revised report shows correction in project description above. SAMPLE DATE CODE DESCRIPTION RESULT " MIN DET LIMIT 1 Fc-129 LOT 564 SIUNROFRAGCATNAINCTSTES--TSMBARSEPORT 2S2E0E0AMTGT/AKCGHED REPORT 5 2 TRITON E66 RH-RM 6-1536 *SURFACTANTS - MBAS 1000 HG/KG 5 3 Las STO. EPA LOT 1138 v *SURFACTANTS - MBAS 60000 MG/KG *5 : 4 DUPLICATE #1 *SURPACTANTS - MBAS 2800 MG/KG 5 5 DUPLICATE 42 ~=====~~~=" SURFACTANTS - MBAS 1200 KG/KG 5 6 DUPLICATE 3 SURFACTANTS - MBAS 60000 MG/KG 5 * = CONTRACT LAB SUBMITTED BY: _(_ Wane DATED: __ 2-23-99 000362 | ) boratories. nc REPORT OF LABORATORY .ANALYSIS Ofices: TtCMiooninsneaeepcoFiliisod, weMiannesota February 10, 1989 M3r. EnWvadieronSmcehnetlall Laboratory 935 Bush Avenue Bldg. 2-3E St. Paul, MN 55133 Gl208 oe Hitfeg wh"i Dear Mr. Schell: ti Enclosed 1s the report of laboratory analyses for samples received If you have any questions concerning. this report, please feel free to contact Tom Halverson or me. Sincerely, Bn Godel! f- RoghrC. Splinter, Ph.D. Director, Laboratory Services . # Enclosures . A ' 000363 : 140 Gouge Dv North Minesprlis, WNs5u5422 Phone 012) 3445543 pace : ries,nc REPORT OF LABORATORY ANALYSIS : OfficTMeaisnm:npeaa.poFlliosr,idMainnesota Nova. Saiowia 3M Environmental Laboratory 935 Bush Avenue Bldg. 2-3E St. Paul, MN 55133 February 10, 1989 PACE Project Number: 890203509 Attn: Mr. Wade Schell G1208 Date Sample(s) Collected: By Client Date Sample(s) Received: 02/03/89 PPaArCaEmeStameprle Number: INORGANIC ANALYSIS INDIVIDUAL PARAMETERS Surfactants (1) Units mg/kg MDL 0G3I221018-0) - 5 2200 0G312210280-2 1000 03G2l123008-3 60000 MOL Method Detection Limit } 000364 1710 Douglas Drive North1qMitnrneoapppolriusn,tMyeNm5i54r222 Phone (612) 544-5643 e--. r ----------------------------eereeeeeeeeeeeOficees: eeseees pace } REPORT OF LABORATORY ANALYSIS VTianmnpeaa,poFlosr,idMainnesota boratories, ne : Elle. Mr. Wade Schell Page 2 February 10, 1989 PACE Project Number: 890203509 PPaArCaEmeStameprle Number: INORGANIC ANALYSIS INDIVIDUAL PARAMETERS Surfactants (1) Units mg/kg MOL am Method Detection Limit Analysis performed by EPA Method 425.1. MDL 0G312210480-4 03G2210580-5 5 2800 1200 0G3220186-06 60000 aThpeprdoavtead mceotnhtoadionleodgiiens.thisAllreapnoarltysweesrewoerbetaipneerdforumseidngbyEPmAeororotuhnedrer my direct supervision. vont, severe Inorganic Chemistry Manager - ] 000365 1710 Douglas Dive Northan0ecMuisnlneoapppoolrtiusn,itMyNerm5pi5o4y2e2r 2 Phone (612) 544.5543 mauysts: nas A : DATE ANALYZED: 2/b[57 AUALYZED BY: Z TDIAMTEA: REVIEWED BY:_2222_ 2% ENTERED BY: BT FOR CLP USE ONLY? TT curent we: PROJECT NUMBER: FPIRLOEJE#C2T NAME: DATE RECEIVED: DATE COLLECTED: snLE | TESICTS] UNITS] COIS sampte | 5 [sa tis BK lookloge] wo |o [<5 std [ico [ug[PCO THES 0 2990005 Jw] 0 lest 22112200 Ingpg| S9210m er Ty oos la 2 212 | hoo wz lis foca [me sus eopool |}[+ 3 his Joses| im az {eco|TV Tpre-2800 * "7 |ig Joou|% Waco [IT| 7 Tis lows] gus jroo JJT1 =vos TigTooes| iz 32 Jeooo|VTTl hag Joes] yg Clghoses.