Document 2np2J9omJzxRrvv382keK5r7

Test Substance: Toxicological Summary Pros Dietary Acute Northern Bobwhite Study Perfluorooctanesulfonate (PFOS) Structure: Remarks: p1o-tOacstsaineusmuslaflot,niCcASa#cid2,7951,.13,92..32.3,3,4,4,5,5,6,6,7,7.8,8,8-heptadecafluoro-, The test substance is stored under ambient caonwdhiittieonpsoawndderpuGriMty wloats#o21r7g)i.nallTyhedestaemrpmlienewdatso bteechn9i0q.ue4s9.% determined Ibny 10 a LsCu/bMsSeq,uen'tH-aHnaMlRy,sis "oFf-tNhMeRestandsubcslteamnecnet,alpuraintaylwysaess be 86.9%. The nominal and measured dietary PFOS Concentration inthissummaryae based on the 86.9% purty value. "vTahleueisn-biafseedphoansethreep9o0r.t4w0%spourriigtiyneasltliymactoimopn.letTedheinre2p0or0t0,waasndrerveipsoerdteidn T2o0x0i4col1o0gicraelfleScutmmtaherysruebfsleeqcutenttheserecvhiasinognes ians wsealmlpale inpculriutdyi.ng resTuhlitss from all of the associated reports of analyses of tissues collected during the in-life phase. METHODS MAeStThoMd:StOanPdPaTrdSE885S07..282700(1(9D9r7a)f), FIFRA Subdivision E. Section 71-2, OECD 205, and Type: Dietary Acute Years 1999 in life tet, 2000 for report, 2004 for amended report Species: Norther Bobwhite (Colinus virginianus) Test Phases: Acclimatio-n10days EPxopsto-seuxrpeospuerrieorde-co5vedrayysp.eriod 3 or 17 days (with th study ending on cither Day or Day 22, respectively) Test quail Age: 10 days old at test initiation rNeuplmibcaetres oinfcRaecphlitcesattessu:bsTthanecree twreeraetmesinxt Trehpelriecawteesreinfitvheequcaoinltrpoelr trerpelaitcmaetnet,(paenn)d ftorwo (towthailcohfi3n0clquudaeidleiinghtthdeosceongtrrooulpst)r.eatTmheentanainmdal1s0wqeuraeilalilnexcpacohsetdesftorsufbivsetadnacyes,tarenadtmtehnetn held for a recovery period. At Day 8, half the replicates from each treatment group (3 of 1 G20583 lrfeplirceatecsofnrtonumetdhenctonhtrosltuadny nt1 lfrDoamyea22c.h dos level) were sacrificed whi the other F{eeneidnganphdasWeastoefr:theFosotdaya.ndTwhaeteqruaiwlerGedprnoovirdeedceiavd alniybitfuom:rmduorfinagniabcicoltiemattrieoantmaenndt rAnialnytiecaltMtonhihatcocrleiinmga:triToenstorsuebssttapnhcaesecoonfctehneirsattuidyo:ns in feed and quail Iver and serum wre iemminedbyreverse phase HPLC and mss spectrometry Sptoaptiusltaicoanl Mfoerthaodgs:enLCeSx0po(sLuertehal(imceo)nceanntdratLiToOn.th(tLetrmeaallstimien 5th0at% rmeosrutlatslitiyn o5f0a% TionmrgeaahsyeurSeodfaibayspiaovpeeurlAaangtaieloyndsaiifsloyrSn8yasgteivm,en(tShdAaotSs/er)SeTsvAuaTsl:uienPs R0wOe%rCePmcoRarOltBcaulIliTat)ty.eodfLb3DySppa0rpo(ubLilettthoaannlaldfyoossis,3 EFvReOnB.IeTx)posuBroedytimwee)igvahluedsaawewreerecalccoumlpaaterdedbybyprDobuintnaentalsystiesst(uSsAinSgSTTOAXTPSRTOACT o`iftthwaErxece(lDwuonrnkseh,ee1t9s55i;ncGluuldlienyg, r1eg9r9e0s)sioAnlslwoitthhrcosnicaetnisttriactalioenvsaloufaPtiFoOnsS wienrteiscuoensd.ucted ARCovaneisrsuaamgnpetDidaoidnlymaonItdnataakkleeti(tAQoDuIaai)lsoobfuonPdtyRpOweSenifgtohrtaedaiacfthfarwweeenarcteemesanvbteergtarwgoeeudnpomwvaaesrletashntedimdfauertmseatdlieoosnn, oaFfpoteohnde xpos period and the ADI was cletated a follows (Equation 1 ADI (mg/kg/day) Aversa weigh =AverageFeedConsumption gi) (Uirdday) pg on (ppmPFOS) (Equation 1) "mTeeastnsDioetfaPHroebpaarrtatmiioxner:, TNheo tceasrtiseurbwsatasnucseedw.asNommiixneadlddiietttaryy ciotnocetnhterafteieodnsaotfioPnFObSy emidlioinn7thpis.stdy were 0 (como, 176, 35.1, 703, 141, 28, 562, and 1125 parts pee RESULTS MGFeaPsRuOrSedinDitehte Ctoentcedinett.ratiTohnes:rWaindipeerceInnttemraetcioovnearly Lodf.fhdeetmeramirneadpickoendcenfterdatiwoanss 5f4r.o7m%5,2w1hoil1e1t9h%e toefsttehendomsianmapllevsaluhea.d mMeesassuurreedd cmoenacenntcroantcieonntsraotifoPnsFOofSPtFhOaSrawnegree:d 000, 187,386,715, 167,279,516, and 1138 ppm. Mreosrtmteantlgsroaunpdi Cgliivniecnal(TaObblseer1v).ations: A summary of he percent morality for cach 2 620584 Teaxbploese1t.do CPuFmuOlSative treatment-related percent mortality of juvenile bobwhite quail Noma Mest AE om PCFonOcS. PCoFnOc.S DIaniialkye. ExposurePerioDdays RecoveryPeriod (ppm) pm) (mgkgdwy1 ) 2 3 4 ST6 7 8 2 Co1nt6rol <L1O8Q7' 1 86 <4L8O%Q 82 000 00 0 00 0 00 0 00[00 0[0 00 0 00 0 000 703 7s 28 0 0 0 0 000 00 18s41 s1l6279 4619749 000 000 0100 2000 250 00 [04[80 181000 S11H00 1 100 efr2asge aT mkaes (RDF i22P5ROSE Bo0yWeig0h per309) b1u0s0ed n10D0a]y10030bod1y0w0eigh1t0n0d f1e0d c$eommamnoinsecuredins cman eel in hecxtended recovery period rom Day 0 Day22. LOimeitqoufaqluawnatsitcautibonni(zLdOoQ)n fDoeryPFOaSfcinstuheandiient gwabsr1o.k10enpplmonDay 5 * One quail was euthanized on Day 3 after sustaining a broken leg. Osunbeseqquuaeinltilnytchuethcaonnitzreodl ognroDuapyof6. 3T0wwoasothfeorunqudaiwl iintahbetrcookhnetnrolleggroonupDhaaydSf,ooatnldewsiaosns hwearte.wenroermaaslsociinataepdpweiatrhanccaegeamnadtebeahgagvriesosriotn.hrouAglhlooutthetrhequatelst.in tThheecroentrwoelrterenaetimtehnetr preaotmmentthireedlinatt.ed mortalities no over: signs of toxicity at PFOS concentrations < 70.3 Ibnrotkheenl1e41gapnpdm tthreeraetmweanst gornoeutpr,eaotnmeenbti.rdelwataesd emuothraanliizteyd(on1Daoy1n3D%aafyt)e7r .sustNaoiniontghear mtooxritcailtiyty(wwiansgnodtroeodp)f.or tHhioswterveeartm,enbt.y DTawyo9a,ddtihteiotnwalo qquuaaiilldihsapdlaryeecdovcleirneidcalansdigatsheiorf haapdpeaarnaonrcmealanadppbeeahraavnicoerarnedtubmeehdavteod nnoorrmmaall.lyAflor tohtehedrurqautaiiolnoinftthhee s1t4u1dyp.pm treatment Ianddtihteon2a8l1 mpoprtmalittrieeastmebneti,ng80o%bsemorrvteadlitfyor(Vth1e0 dquuraait)iownasofobtsheervteesdt. by QDuaaiyl7mwoirttahlintoy orucfcfulrerdeadpopneaDraanycse,5,r6ednucded7.reSacitginoson ftotosxiimciutly (wseoruendoabsnedrvmeodtpiroino,r 0letdheaartgyh,awnidnigncdlruodoepd, laodssnoofrcmoaolrdaipnpaetiaorna,ncleowaenrdlbiemhbavwieoarkfnoerstsheanddurcaotnivounlsoifotnhse. stAufdtye.r Day 9, surviving quail IDnaythe7.56S2igpnpsmofttroexaitcmietnyt,obmsoertravleidtyprwiaorsofirdsetaotbhseirnvceluddoedn aDaruyff3leadnadppreeaarcahnecde,1r0e0d%ucbeyd rweeaacktnieosns,tolostwiemrullii,mblertihgairdgiyt,y,dperporsetsastieonp,oswtuirnegadnrdocopo,nvu10l5ssioonfs coordination, lowes limb 3 : 600585 In the Day 4. 1125 ppm Signs of ttorxeiactimteynto,bsmeorrtvaelditpyriwoars(0fidrestatohbsienrcvleuddeodnaDrauyffl3edanadppreeaarcahnecde,1r0e0d%ucbeyd rweeaacktnieosns,toansdtimluolwie,rlleitmhabrgryig,idditeyp.ression, wing droop, loss of coordination, lower limb BdiottahryLC8S-0dsayaLndCSL0TSw0ass f2o1r2PpFpOmS PwFeOreS dweittehrami9ne5d%bcyonpfriodbeintcaenailnytseirsval(Toafbl1e582).to T27h8e pcpomn.sumpTthieonslodpaetaocfotlhleeccteodncdeunrtirantgiotnhere5s-pdoanyseexcpuorsvuerewapseri7.o0d4.werBeoduysewdeitoghetstainmdatefoaond average daily LDSO based oinntaAkveeroafgPeFDOaSilayndInatnakeadd(iAtDiIon)alofproPbFitOSanawlaysses61wamsgcoPnFduOcStKekdg. The body w"Tehieghstliodpeaoyfwtihteh daos9e5-%rescpoonnfsiedecnucreveinwtaersva8l.8o8f. 4T8hetoL7T7S0m,gesPtFimOaSt/ekdgwibtohdythweei1g1h2t5idpayp.m `PwFhOerSe t3r0ea%tmmeonrttadlaittay wwaass 3o.b0s6erdvaeysd.at Tdhaiys3reasnuldt 1a0gr0e%edmowrittahlictuymwulaastiovbesemrovretdaliattydadyat4a (Table 1). Toafablpeop2u.lEasttiiomnaotefsojufvtenhielelneothratlhecronncbeonbtwrhaittioenqouafilPs.FO*S (LCso) and the lethal time (LTso) `Effect metric Time LC50 (ppm) 95% CL Slope. LC50 Day3 Days 1,593 82 356-672 3174 am Day 6 Day $8 319 212 228-448 158-278 3923 7.03 Day22 212 158-278 7.036 750 Dose 281 ppm LTS @ay) 641 95% CL 5697.61 Slope. 954 562 ppm 1125 ppm. 478 3.06 417.548 . 829 605 TT by FST GATS PROCTOR BbooddyywWeeiigghhtt:foIrnqucaoimlpsarinistohne t1o7.c6o,nt3r5o.l1s,otrhe7r0e.3weprpemnotraepaptmaernetnst.PFHoOwSevreelra,teqducafiflecitnstohne 141, 281 and comparison to S62 ppm controls treatments had body weights (p<0.01) for Days 0-5. While that were there was significantly a statistically reduced in significant rweadsucntoiolnoningebrodapypwaereingthtbyatDPaFyOS15c(on1c0endtaryastipoonsst >ex7p0o.s3urpep).m, tAhsiswbaosdyobwseeirgvhetdrfeodrucbtoidoyn. woebisgehrtv,edsigatnifciocnacnetnttrraetaitomnesnt->re7l0a.t3edpepffmectPsFOoSn.bodHyowweeviegrh,t agfatienr fDraoym D8,ayno0 ttroe5amweenrte- treerlmaitneadtieofnf.ectsEffoenctbsoodny weight gain body weight owrerbeodynowteeidghatt any gain concentration could not be through study determined for 281, 562 and 1125 ppm treatment groups after Day due to most of the quail dying in these groups. a G50586 dTiaebtlaery3.coAnvceernatrgaetbioondsyowfePiFgOhSt.()ofjuvenile northern bobiwhites exposed to various Treatment Body Weight Body Weight Body Weight Body Weight Body Weight (Cpopnmt)ro.l 176 Da2y00+7* 2141 0Day4s ea Deays8 ass PDa1y 0IS 828 RDayD22 [i 730531 020:211 3310s22 33792233 6052x54 78992247 218411 22002=11 ewsese dIsessa 832s3 795252 15262s 2200s211 16m022 mnm mm mm naBaotynttweaenpaclTioedpnuof0lom1m0c0eo%mmeoSgnrloauypnndre0s.Ge0nt(e rDpuonmo Tmee8r)r. e. vInariosuescosnpdanssetooffatniamleysdeusr,intghetghreoswttuhdyr,atienscflourdienagch0-t8redaatymse,nt8g2r2oudpaywse,reancdal0cu.l2a2teddayfsor GVirnoewartihzerdaatend(1tdheany)fiweadsteostthiemabtoeddywwithea dgiaenteagrcaollhlgercottwetdhdumroidneglth(eEqsutuadtyi.on 2) that was W, = Woep (Equation 2) `Where: W`Wyoiisstthhee bbooddyy wweeiigghhtt ((gg)) ooffaammaallllaarrddaatttthiemeon(ste)tofotfthheessttuuddyy (1=0) gissthtiemeo(vderaaylsl)growth rate (1day) dDiaetdabfyroDmayth8e.SF6o2raDnady101-285, pthpemgPrFoOwS rtarteeasotmfetnths wqeuraielnionttirnecaltumdeendt,gsrionucpsaolfl qlueaslthhaadn a7g0.r3eepmepnmt wweirthe tnhoetbsotdatyiswtieciagllhyt ddiatfaf,reqnutalfrformomthteheco1n4t1rolangdro2w8t1hprpatme t(rTeaabtlmeen4t).s haIdn aDfateyr 0D-a8ygr8o(wDtahyr3ateosftthhaetrweecroeverryedpuecreidodf)rtohme tqhuaatilobisnetrhveed14i1n atnhed c2o8n1troplps.m tHroewatemveenrt,s hgraenwoart eaquaatletoth7t0.w3apspemquPivFaOlSe.nt oThethaotveorbalslergvreodwtinh qruaatiels ffoiromquatirleatimnentth.e g1r7o.u6,ps35l.e1s,s c7o0n.t3r,ol14g1r,ouapn.d 281 ppm treatments did not differ from the observed growth ates in the 5 : GNO587 `Table 4. Growth rates (l/day) for juvenile northern bobwhite quails exposed to PFOS. A Days 0 176 Treatment group (pm) 351 703 141 281 008 81022 T0803 00549 0.0803 00555 00839 00589 00773 00542 00623 00624 0.0140 007% 01022 00639 X Grorateswcuitmathed by incar 00681 regression: (Ln 00681 Body weight 00628 (9) growtathe 00648 0.0526 (me)+ y-imereep). FtheeedexCpoosnusruem(pDtaiyosn:0-5T)heorrerewceorveernyopePrFiOoSd-(reDlaaytse6d-2ef2f)oecfttshoensfteueddy.cAonrseudmupcttiioonn idnurfienegd cexopnossuumrpetiopneriwoads noted (Days a0t-5t)hebu2t81,the5s6e2 and 1125 reductions ppm were tnroetatmsetnattisgtircoaullpys during the significant. `bOovderyalwle,itghhetijduaveynfiolretqhueasltuadvy.eraWgheidlea tchoenrseuwmapstiaonslirgahtteodfecraepapsreoxiinmtahteelfyee0d.c2o9n8sgumfpeteidogn rate during the recovery period, this result was observed in all treatments groups, including the control. Table 5. Average feed consumption (FC)of juvenile northern bobwhite quail exposed to dietary concentrationsof PFOS Treatment (gpm) FC Days0-84 FC Days 8.22 FC Days 0-22 (C1on7t6rol) 00331215 0011872 0218 0211 351 03 0317 0401 0227 0241 0263 0280 141 281 0347 0376 0219 0230 0227 0282 562 0.681 12s 0200 NA NA NA NA TcFNRoAvern=aNgoestfaepupdlicmablpe dute toi100voe%nnmasorJalibtyo.dy weaona pnb GthreosrsemNaeicnrionpgsbyi:rdHsawlefroefntehcerospusriveivdinogn qDuaaiyl 2w2erfeolsluobwjiencgtetdesttotneermcirnoaptsiyono.n ODnayDayan8d, oconnesibidredreidn ttrheeat7m0e.n3t-rpeplamtetdr.eatAmesnitngwlaesbinrodteedutwhiatnhizaedslfirghotmlytphael2e8l1ivperpmbuttrtehaitsmewnatswnaost ocobrsreerlvaetdedtowihthavetreaatlmaecnkt-roeflamtuesdclefefemctasssonanbdodgyenewreailghtth,inntehsiss. necSrionpcsey thfeisnedinfgindaisng cnoencsriodpesryedfintdrienagtsmetnhtatrwelearteedu.nrTemhaerkraebmlae.ining quail that survived to Day 8 and 22 had Adleladqudauirlitnhgatthdeiesdtuddyu,ritnhge tnheecrsotpusdyy wreesrueltssuibnjeccltueddedtothaingrcoosnsditnieocnr,oplsoys.s oFfomruqsucaliel found mass, aclotnesrieddersepdleteonbceoltorre,atamuteonltysreilsatoefd.tissues and pale organs (Table 6). These findings were: 6 na588 `Table 6. Gross pathological observations study with PFOS. Finding "Abdominal cavity, some autolysis Abdominal cavity, autolysis throughout Crop, empty EmFraaccitautreeddleg GI. tract empty Gizzard contents bile stained `Heart, anterior portion mottled white color Heart pale Intestinal contentstar lke: Keel, prominent LKiivdenre,ysp,alpeaalend mottled Loss of muscle mass Muscular-skeletal,pale Small in stature: Spleen, black Spleen, small and pale or both Spleen, dark, grey or grey-brown `Thin Not Remarkable from quail Conwol N=) 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 that died during the dietary acute Treatment 141 (Nd) 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 Group (ppm) 281 S62 1125 (NB) ON) N-10) 227 01 1 2s2 2 5s 8 0 [I 1 10 251 100 02 1 02 0 1 30 020 oo 0 0 479 0 [I 3 [I 010 132 012 042 1 o_o Concentrations of PFOS in Tissues: Liver concentration data are presented separately for quail that died during the study and for those sacrificed at study termination. Serum data were also collected from quail at study termination. Liver samples collected from quail that died during Days 0 0 7 of the study were analyzed for PFOS content, and the data are presented in Table 7. During this period, mortality in quail was associated with liver concentrations that were greater than 110 ug/g PFOS. In the 1125 ppm PFOS treatment, this concentration was achieved by Day 3, while for quail in the 281 and 562 ppm treatments, this level was achieved by Day S. Since birds were not sampled in a systematic manner, a pharmacokinetic analysis of the data could not be conducted to evaluate the uptake and disposition kinetics of PFOS in these quai. 7 670589 Teaabtlmeent7..reMleaatseudrmeodrtcalointcyendturraitnigontsheoafcuPteFOdiSet(auryg/sgt)udyi.n *the liver of select quail with Day Tat "Nomina2l81PFOS ConcentratiSo6n2s (pom) 1125 3 Nomorally Nomorally | Notamlzed 138 (ND) 45 NNoommoormaalltyy N2o0m9o(mNa-lDt)y N1o26ta(mNl-y1e)d 126-(N-3) 6 7 NL omomQaliy 13290(2(NN--2) 213151(8N1=)) -. quaAilllhPtROdSedcoinnce3ntrreasttiomnnstrgerpoourtpeodninsupneictisfioefdWdaEy.,Twhetnwuemibghetr iTnphrectdhaeasirseprestehen naunmabveerraogfesaomrplaels nly an no themmr ofbirds st wer ubjeidfo aalyss. Somesampleswere composi. R8easunldtsDafyo2m2lsivheorwantdhatsePrFuOmSanraelmyasienseodfpsraesmepnltesafceorlltehceteedxfporsoumresuprevriivoidng(Tqauabille o8)n. DNaoy mortality was associated with liver concentrations = 450. pg/g PFOS or serum concentr=a41t.2ipogn/msl PFOS. Tcoalbllecete8d. aMteDaasyur8edancdoDncaeynt2r2aotfiotnhse osftuPdFy.OS in the liver and serum of bobwhite quail Day Dayz DospemGr)oup T(i5/v0e)r Segr/umm TGievoe)r Segr/umm) Control <L0Q <L0Q 0212009 0122025 351716 21588552436756 30<4053Q92 3229525+018516 518297+2142187 710431 40740346 4a1s2i43a1]1 215080e4638249 20652:421445 T * Lio2of1quentiaion (LOG)N.ALOQ= 0008 gm4rE 6um),LOQ-0.033l9g3 (iver) -- i NA ot splice samples were ntcollected q"Tuhseilawvearsag0e.5r1a5toonoDfacyonc8enatnrdat2i.o4n2soonfDPaFyO2S2.inTbhleoordelasteirvuelmy tgoretahtatdiifnfelrievnecre(bSeLt)wefeonr stheeruDmaytha8tawnadsDoabyse2r2veSd:Lacrraotsiso awlastrdeuaetmienntpargtrotuopst.he Ilnowpacrotniccuelnatrr,attihoensseorfuPmFPOFSOiSn cqounacneinttartaitoinontshusinrtehdeuci17n.g6thaendS.L3.5.r1atop.pmFitnralelayt,mehnetrgerwoueprse nweorseiglneisfsictahntanditfhfeerleinmcietsoifn ttohethSe:Lsmraaltliosbaemtpwleeensimzealaendanrdelfaetmiavelleymgarlelaatrdvsa.riaTbihleitlyactkhaotfwsaigsniofbiscearnvceedwiansbdouthe sinerpaurmt raenpdortlievdersepPaFraOtSelyc.oncentrations for all treatments. Therefore these ratios were not TcohnecenrterlaattiioonnsshmiepassubreetdwoenenDayPF8OaSre gtirveeantment dose and liver and sem PFOS 8 60590 Liver PROS(ug/g) = 0.5087*(Dose, ppm-) 3.0744 R= 09585 Serum PFOS (ug/m) = 0.3399*(Dose, ppm) ~ 0.6851 R-03985 AcosncewnittrhattihoensSoInL Draatyios3,. thTehries wwearse dnuoe tsoexthsepescmiaflilc sdaimffpelreensciezse ianndserreluamtivaenlyd glrievaetr svaarmipalbielistiyzeobwsaesrvdeudcinintphearsetroutmheanfdactlitvehraPsFeOruSmccoonncecnetnrtartaitoinosn.s wFeorre sneortumm,eatshuersemdaliln bids that died prior to Day 8. The variability in the data is alo reflected in the relatively csomnaclelntcroaetfifoinc.ienTthoefrdeeltateiromnisnhaitpiobnet(wRe)enfoPrFtOhSe dsoesreumanPdFiOsSsuceocnocnecnetrnattriaotnisontsootnreDaatyme2nt2 rweelraetionnosthipasn,alaynzdedthseinicnefogrrmoawtitohnagnadindeedpuwroatuilodn nmoatybehaavpeplisciganbilfeictaontleynvailrtoenrmeednttahle situations pPoesrtioedxp(oDsauyrse,2t2h)e.conTcheinstrdaetciorneaosfe PwFaOsSmoisntthleikleilvyerdudeecrteoatswedo tfhacrtoourgsh,ogurtotwhtehrdeiclouvteirony aisndtheexcsraemteiona.t lIonwearddbitoidoyn,biutrwdaens laesveslus.meTdotheasttihmatbeiotlhoegilcoasl moefcPhFaOnSisfmrfoom leilviemridnauteiotno d"ehpeurfaotliloonwifnrgommodqeualile,vaalusaitmesplteheonraet-eocofmlpoasrstomfenPtFOmSodferlomwalisvere:mployed (Equation 3). Co=GrewTM (Equation3) Where: CCaisistthheeccoonncceennttrraattiioonnaattthimeon(s1e)tduofreilnigmtihnateiloimni(n1a8t/i8o)n phase (g/g) ct'isstthimeeo(vdeayrsa)l elimination rate constant (day) "cTohneceonvteraatliloneliinmilniavteironplaotteedcoansstaanftun(ckt"i)ownaosfetsitmiemadtuerdinbgy tdheetecrlmiimniinnagtitohneplhoapseeoofftthhee scotnusdtyan(tDatyaskes8-i2n2t)o. acIctouinstilmopsosresanftrotmo trweomecmobnecurrrtehnat ptrhoeceesssteism,atgerdowetlhimidinlauttiioonn raantde eFxocrretthiiosna.nalTyhseisse, ittwwoaspraocsessusmeesdmtahaytdbioftfhrpirnociemspsoerstaanccteedbianseadn oinndeagpee,ndseenxt, abuntdasdedaistoinv.e manner such that the over all climination o loss could be representeda follows: Kelty Irnectohridseedqudautiroinn,g thies tshteudgyrowwhtihlerakt'thwaatswaessteismtaitmeadteads ftrhoemobvoerdayllweriagtehtomfeadsecurreeamseentisn mweaassuesrteidmaltievder(PksF).OSBcaosnecdenotnrattihoensm.eaOsuneeedkl*ivaenrdPgFOweSrecocnaclecnutlraattedi,onhseanedligmirnoawttihonrartaetse f1i7.o6m,3D5a.y1s,70t3h,ro1u4g1h 2a2nodf28s1tupdypmperPiRodO,Seltirmeiantamteinotnsr(aTtaebcloens9)t.antTshweearveeersatgiemaetliemdifnoartitohne 9 r0591 reastteimoaftePdFOtoSbe(baapsperdoxoinmaltiveelryco1n2.c8endtaryast.ions) was 0.0542 day' with the haliff in liver Table 9. Growth rates, liver PFOS elimination constants and excretion rate constants for PFO in bobwhite quail. * Treatment "Growih Rate T 3 (ppm) (day) (@ay') ay") (Co1n7t6rol) 00.0055959 0.1=253 0.0=698 0313 0000558892 00..11615023 00..01806613 281411 00..00769284 00..00893810 00.00128027 peRrTiTod Daycosns8t1a0n2ts).were based on dts derived Tom essrerent adeQui he recovery o`Sbesreurmvecdoncfeonrtraltiivoenrs oPfFPOFSOScodnicdenntortatbieohnasv.e inDaurmianngnerthtehatrweacosvecroynsispteernitodw,ithmetahant concentrations of PFOS in the serum increased from <LOQ 10 5.89 g/ml and from 3.04 1co0n7c.e2n7traptgi/omnls iinn stheeru1m7.o6faqnudail35.f1rompptmhet7r0e.a3tmepnptsm, trreesaptemcteinvteldye.creIansecdontfrraosmt,41P.F2OSto 2in62memagsu/rLeodvesrertuhme scaonmceenttirmaetpieornisodo.n IDnaaydditwioanstroetlahteisveeliyncgornesaitst(enCcVies>, t1h0e0v%a)riafboirliatlly rtreecaotvmeernyt pgerroiuopds., mAaskinagreitsudlitf,fikciunletti0c eavnaalluyasteestrteoatemsetnitmarteelactleedardainfcfeeretnicmeessdwuerrinegntohte conducted with the serum data. CONCLUSIONS: o`Tnhe AdiDeItarwya8s-da6y LmCgS0 PwaFsOS2K12gpbpomdy(CIw=ei1g5h8t-d2a7y8 p(pCml) w4h8i-l7e7thme g8-dPaFyOLSDkSg0 bbaosdedy `weightday). The no mortality concentration was 70.3 ppm (or 23.8 mg PFOS/kg body wAedivgehrtsdeayE)f.fectBaCsoencdenotnratthieonre(dNuOctAiEonC)ofwabsoddyetweerimgihntedattoDabey 780.,3 thpe pNmo(oOrbs2e3r.v8abmlge (PLFOOASEKCg) bboasdeyd woenigbhotddyayw)e.ightThateDLaoywe8swtasOb1s4e1rvpapblme(eAqduivvearlseentEtfofe4c4t.7CmogncPenFtOaStkigo.n body weightday). The 8-day No Observable Adverse Effect Level (NOAEL) and wLeorwees4t4.O0bsaenrdva70b.l3 e uAdgv,ersreespEefcfteicvtelLy.eveTlh(eL8O-AdEaLy)NOvaAluEeLs baansdedLOonAEliLvervaclounecsenbtarsaetdioonns sneereudmedcownhceenntrianttieornpsrewtienrges4e1r.2umanPdF4O1S.5vgal/uems,rerleastpievcetivteolyc.ondHuocwteivnegra, sriosmkeascsaeustsiomnenits with monitoring data. The source of this caution is duc to the fact that serum PFOS `lceovneclesndteractrieoansseddiddunroitngfotlhleowretchoevefrryenpdhaosbes.ervItediswaisthsulmiveedr tchoantcetnhterabtiioolnosg,icwahlemreeicnhaPnFiOsSm fHoorwecvleirmi,natthieodnatias itnhdeicastaemtehatatthlisowaesrsubmopdtiyonbumradyennoltevbeelstrufeoranbdotthhatlitvheer maencdhasneirusmm.s 10 "0592 otfherlesihmoilndatvailounesfr(ogmeomtehtesreictmweoatniossfueNsOmAaEyLnoatndbeLOsiAmEilLa)r.arTehseumNmOaAriEzLe,d L(TOaAblEeL,10)a.nd `Table 10. NOAEC, LOAEC, and threshold doses (TD) of PFOS derived franoorthmern bobwhite qual acute feeding study * Endpoint NOAEL _TOAEL TD Dietary (ppm) Average Daily Intake (mgkgiday) 703 23.8 441471 21063 Serum (ug/m) a2 as ara LiAvTerval(suggw)ere sed on the eductionofbody4w4eig0ht 703 Geatd quail 556 Liver 1nd NOSeAruEmLPRanOdSLcOonAcEenLtrvaatliuoenssfaorredbiaesenddontDheaAyD8Idaats. reporid a dietary concentrations Sera andlivereffect values ar measured issue valu. DATA QUALITY: Reliability: Klimish rankin=g 1 REFERENCES: Dunnett, CW. 1955. A multiple comparison's procedure for comparing several treawtimtheacnonttrosl. J. Amer. Statis. Assoc. 50:1096-1121 Gallagher, S.P., Casey, C.S., Beavers, J.B, and Van Hoven, RL. 2004. PFOS: A dietary PrLoCjSe0ctsNtuOd.y:w4i5t4h-1t0h3e.Northern Bobwhite. Amended report, Wildlife International Ltd. Gulley, D.D. 1990. TOXSTAT Release 3.2. The University of Wyoming. Hansen, K. Bobwhites 2001. (Sera Analytical and Liver). Report of Revision Data for PFOS Dietary 1. 3M Environmental LCS0 Study Laboratory. in Norther Report No. FACT-TO1X2-5. SAS Institute. NC. USA. 1999. SAS/STAT User's Guide, Release 8.02 Edition, SAS Institue, Cary Wagner, JG. 1979. Fundamentals of Clinical Pharmacokinetics. Drug Intelligence Publications Inc., Hamilion, 11. 461 p. OTHER: Last changed: 5105/04 n 600593