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ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE______________________________________
Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3003. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not given. The test sample is FC-3003, identified by the laboratory as R1331. Current information indicates it is a mixture of 1% PFOS, 15% diethylene glycol butyl ether, 79.39% water, 2% Sultone foamer, 1.5% sodium octyl sulfate, 1% sodium decyl sulfate, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:__________________________________________________________
Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1997 Species: Daphnia magna Supplier: In-house cultures maintained at AScl-DETD, Duluth, MN. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: No calculations performed, based on study results. Test conditions:
Dilution water: Dechlorinated City of Duluth, MN tap water post carbon filter, aerated for 24 hours prior to use. Dilution water chemistry:
Hardness: 44 mg/L as CaC03 Alkalinity: 42 mg/L as CaC03 Conductivity: 145 pmhos/cm pH: 8.05
Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test solutions were prepared by direct addition of test substance, followed by mechanical stirring at ambient lab temperature. Exposure vessels: 250 mL beakers with 200 mL test solution. Number of replicates: 4
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Number of daphnids per replicate: 5 Number of concentrations: one plus a blank control Water chemistry during the study:
pH range (0-48 hours): 7.89 - 8.11 (control exposure) 7.88 - 8.05 (1000 mg/L exposure)
Temperature range (0-48 hours): 20.2 - 20.7 C Conductivity range (0-48 hours):
1 3 3 -1 5 4 pmhos/cm (control exposure) 1 5 0 -1 6 0 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48 hours): 8.7 - 8.9 mg/L (control exposure) 8.6 - 9.1 mg/L (1000 mg/L exposure)
RESULTS______________________ ________ =___________________ _
Nominal concentrations: Blank control and 1000 mg/L.
Element values: 48-hour EC5o = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS____________________________________ -__________
The test substance 48-hour EC50 was determined to be >1000 mg/L. No mortality or sublethal effects were observed.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY_______________________________________________
Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES________________________________________________
This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1331, 1997.
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OTHER____________
Last changed: 6/26/00.
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AScI Corporation/AScl-Duluth Environmental Testing Division
AScI Study ID# 5010-068
STUDY TITLE ACUTE TOXICITY OF R1331 TO DAPHNIA MAGNA
DATA STANDARD
OECD GUIDELINE 202 "
&TyDY_CQMPLEIBD
March 27, 1997
TEsum EACILHY
AScI Corporation/AScl-Duluth Environmental Testing Division
4444 Airpark Boulevard Duluth, MN 55811
Tel. No. (218) 722-4040 Fax No. (218) 722-2592
STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068
3M Company Lab Request# R1331
Sponsor: 3M Company Sponsor Study ID# R1331
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1.0 GENERAL INFORMATION
AScI Corporation/AScI-Duluth Environmental Testing Division
AScI Study ID# 5010-068
Study Title
Data Standard Sponsor
Sponsor's Representative
Sponsor's Lab Request# Testing Facility
Principal Investigator - . .. .......... -.... Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review
Acute Toxicity of R1331 to Daphnia magna.
OECD Guideline 202.
3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133.
Susan A. Beach, 3M Environmental Technology and Safety Services, Building 23E-09, 935 Bush Avenue, St. Paul, MN 55133. Tel No. (612) 778-7452.
R1331.
AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040.
Tina Galloway.
Joe Amato.
Donald Mount.
March 20-22, 1997.
Alan Mozol.
RepprtSignatu^e: "
Retention of Raw Data and Final Report Location of Raw Data and Final Report
Two years from the dati study is completed.
AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040.
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Sponsor: 3M Company Sponsor Study ID# R1331
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AScI Coiporation/AScI-Duluth Environmental Testing Division
AScI Study ID# 5010-068
2.0 OBJECnVE(S)
To determine the 48-h median effect concentration (EC50) and 48-h no observable effect concentration (NOEC) of the test substance, R1331, for Daphnia magna.
This study was conducted according to the AScI study plan retained by the Sponsor and OECD
Guideline 202 (OECD 1993).
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Test Substance
Test Organisms
Dilution Water Exposure Chambers
3.0 TEST METHODS
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Properties: Clear amber-colored liquid, specific gravity = 1.02 (water = 1), completely soluble in water, and stable.
Test concentrations: 0 (dilution water control) and 1000 mg/L. High concentration test solution was prepared by mass addition followed by mechanical stirring at ambient lab temperature.
Source: Healthy, less than 24-h old Daphnia magna neonates obtained from AScI stock cultures maintained in water of test dilution water quality!
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Use in test: Gravid adults isolated in dilution water 24 h before the test / initiation. Used 5 organisms/replicate and 4 replicates/test concentration. T Handled with a smooth bore pipet with an inside diameter of 6 mm. Did, not feed during the test.
Source: Dechlorinated City of Duluth, MN, tap water. The most recent
chemical analysis is in Raw Data Package.
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Characteristics: At test time, the water had a hardness of 42 mg/L (as CaC03) and a pH of 8.11. Aerated for 24 h prior to using in the test.
Type: 250-ml borosilicate glass beakers.
Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made.
Sponsor: 3M Company Sponsor Study ID# R1331
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| Incubation Observations
Endpoint Calculations
AScI Corporation/AScI-Duluth Environmental Testing Division
AScI Study ID# 5010-068
Duration: 48 h.
Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent lamps.
Temperature: 21 C.
Biological: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure chambers were gently agitated). Did not remove any daphnids from the exposure chambers at 24 h.
Water chemistry: (1) At test initiation- total hardness, alkalinity, (2) dailytemperature, specific conductivity, pH, and DO. All determinations were1 made according to APHA methods (1995). Formal calculations not needed due to lack of effect.
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4.0 RESULTS
Test Substance Toxicity (Pertinent data are in Table 1)
Ranges of Water Chemistry Parameters
24~h EC50: > 1000 mg/L.
48-h EC50: > 1000 mg/L.
48~h NOEC: 1000 mg/L.
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Total hardness (as CaC03 mg/L): 42-44. Alkalinity (as CaC03 mg/L): 40-56.
Temperature (C): 20.2-20.7.
DO (mg/L): 8.6-9.1.
pH: 7.96-8.11.
Specific conductivity (pmhos/cm): 133-160.
Sponsor: 3M Company Sponsor Study ID# R1331
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5.0 TEST QA/QC
AScI Corporation/AScI-Duluth Environmental Testing Division
AScI Study ID# 5010-068
QA/QC Criteria
Test organism dead, immobilized or trapped in control exposure must not be more than 10%.
The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature.
Test duration must be 48 h.
Reference substance (sodium chloride) toxicity test.
Data
0% dead, immobilized or trapped.
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The lowest DO concentration measured was 97% of the air saturation value at 21.0C.
The test duration was 48 h.
AScI's updated reference toxicity test control
chart is in the Raw Data Package.
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6.0 REFERENCES
American Public Health Association (APHA). 1995. Standard Methods for the Examination o f Water and Wastewater. APHA, Washington, D.C.
Organization for Economic Cooperation and Development (OECD). 1981/1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C.
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Sponsor: 3M Company Sponsor Study ID# R1331
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ASci Corporation/"ASc[-Duluth Environmental Testing Division
AScl Study ID# 5010-068
TABLE 1. ACUTE TOXICITY OF R1331 TO DAPHNIA MAGNA
Test Subttutce Concentration
0 1000
* Percentages are in parenthesis.
Cumulative Number and %ofDead and Immobilized Organisms* 24 b 48 b
0(0) 0(0) 0(0) ____________m ____________ 1
EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS
4 and NOEC '
24-h EC50 48-h ECS0 48-h NOEC
Data (mg/L)
> 1000 mg/L
> 1000 mg/L 1000 mg/L
95%ConfidioeIiiterval Not calculable Not calculable Not applicable
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Sponsor: 3M Company Sponsor Study ID# R1331
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