Document 2jyM3OJjnOwRLm4yr01RgVGrr

ACUTE TOXICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 1. The testsample isFC206 (L-3243).Currentinformatioinndicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether,78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylene monooctylphenylether,and 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations ofimpurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute static. GLP: No Year completed: 1974 Species: Salmo gairdneii Supplier:Not given. Analyticalmonitoring: Dissolvedoxygen,and temperature Exposure period: 96-hours Statisticamlethods: LC50 valuecalculateudsinggraphicinterpolation. Test fishage: Juveniles Length and weight: Loading: Not given. Average length 38.1 mm Average weight 0.9g (wet) Pretreatment:None. Test conditions: Dilutionwater: Dechlorinatecdiiywater,St.Paul,MN Dilutionwater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:Not given. Concentrationsdosing rate:Once Stabilitoyfthe testchemicalsolutions:Not noted Exposure vessels:Not given. Number ofreplicates:1 Number offishper replicateN:ot given. Number of concentrations:fiveplusa blankcontrol Water chemistryduringthestudy: Temperature range (0-96hours):50 OF Dissolvedoxygen range (0-96hours): 4.9- 5.9mg/L (controelxposure) 4.0- 5.2 mg/L (3000 mg/L exposure)* 3000 mg/L (intermediatceoncentratione)xposure values used because the highesttwo concentrationsresultedintotalmortalitbyy 72 hours. RESULTS Nominal concentrations: Blank control2,000, 2500, 3000, 3500, and 4000 mg/L. Element values: 96-hour LC50 = 2500 mg/L Element valuesbased on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206 96-hour LC50 forrainbowtroutwas determined tobe 2500 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordof methodology used and documentation. The sample pudtywas not properlycharacterizedand the studylacksanalyticaclonfirmatioonftheamount offluorochemical proportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, 1974. OTHER Last changed: 6/28/00 7401ol EWIRONMENTAL AQUATIC ';-14 '7 F-@ ENGINEERING LA80ii@T-@RY-' TOXICITY WORK SHEET l@t v Type Test 5 rig r /'c - If- I %A- Test Organiam A -. . 7-ro,,7 Date Started -7el Material Teste@Z Z 3.3.22 -7,3 2- Avg. Weight i@zSS izee Date Completed Time Started- 0 -------- Plotted LCso- ;2 Dilution Water -ct,, - - Conc. 24 hrs % Survival Control ic)o rtjff .?,oD-o loo .2,5'oo3)t>Od 1'66 Cl0 48 hrs 72 hrs D.0 t D.0 % 96 hrs D.0 m Survival H a Survival PH __ppm survival pH Dpp.m0 joo too 6,3 too 1-717 co 10 too e47, / oo ioll o 6.z 20 Average Temperature 10C0IO LCSO Calculation 40 co 41 3o-@ r- )K t t 41 10 T 0 16' 2 30 40 So 60 70 8.0- IIoo Survival