Document 2jaOz93y6j3d4mND1Znrozoa5
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DEPARTMENT OF ZOOLOGY' ` P.O. DRAWER Z STATE COLLEGE, MISSISSIPPI 39742 PHONE (401) 3Z3-UZI, EXT. 305
8 February 1967
Mr. L. C. Fuhrmeister '
Technical Servic sc Superintendent
Monsanto Chemical Company
Amiscon3 Alabama 3201
<\Z>A <' ? ^At Oc=r&iZ-c~`
7T>f^ f*"*nv 1
o&
bear Mr. Fuhrmeister:
Enclosed is a description of our progress report.
Vfe have been unable to demonstrate any reason why large fish kills should occur in Choccolocco Creek.
I received your notice of Mr. Carder's visit. Since I teach two classes on Thursday morning, 1 am hoping that it will be convenient for me to meex with you on Friday the 24th at 8 Av. Should this he impossible, I will attempt to make arrangements for Thursday.-
-Enclosure IEF:vb
Professor of Zoology*
:rO (*
DSW 162370
I* EXHIBIT
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WATER_PCB-SD0000017562
DEPARTMENT Of ZOOLOGY P.O. DRAWER Z STATE COLLEGE, MISSISSIPPI 397S2 PHONE (SOI) 323-4321, EXT. 303
Progress Report - January
On January 24- dissolved oxygen measurements -were taken at several sanding sixes "by ike "rfiuklez Mexhod. viatsr temperatures and. pH--values were recorded at the same xime. ' Th.es e eata are summarized "below:
Location
rime
"water Temp. (C)
pH P. 0. (ppm)
Surface
Elbow Pepth
1. Choccolocco Creek: Bridge -- 4 mi. E.
of Boiling Sxdugs
11 A.M.
.
2. Anniston Sewage Treatment Planx; (Pinal tank}-=-
12 A.M.
3.. Monsanto Sewage
2 P.M. '
Treatmenx Facility -
ax ouxfall to city.
4. Snow Creek -
2:30 P.M.
r.*.`!'r.T>sl 1 "fVrrrn VnncAnr.O
Planx (Front ditch
at fence)
5. Choccolocco" Creek 3:30 -P.M. at Hiway 93 Bridge
l4 7.8 10.8 10.0
21 7-2 3.8
31 7.6 4.7
34 0.7 " Hone Petected
Jr*
** . SW
14.5
7.5
8.5
. 8.5
* The "basin contained a large amount of -foam.
not measured.
The D. 0. values for the Choccolocco Creek sites axe veil within
sale limits. Barring adverse effects of detergents and low nocturnal
L.G.-values fish might even survive in the Anniston Sewage Treatment
Planx. Although the front ditch from the Monsanto Plant contained no
measurable P.O. and had a very low pH, (Our tests have previously
shewn xbax "blnsgills die in 20 minutes at pH 3-0) "both pH and P.O.
ere normal at the dovnsxream Choccolocco Creek site (Hiway 93 Bridge)..
DSW 162371
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WATER_PCB-SD0000017563
u auuarylSrogresslSepaft' rage 2
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vie have corsletsd preliminary studies to determine possible synergistic action of mercury end parathion. On January l8, four 15-rnllor- acuaria were prepared with the following test solutions; 1} 20 liners of 100 ppb HgCl2 solution; 2) 20 liters of 100 ppb p=rariion3- 3) 20 liters of solution in which both HgCl^ and unrathior. vere nresent at 100 ppb concentrations3 4) 20 liters of rap water as a control- Each aquarium was stocked wi-ch 25 medium sized green sunfish (Leuamis cyanellus) -
Th.e following table shows the number of survivors.
100 ppb EgCls
.c. Pish
12 hours 2t- hours 36 hours 60 hours
25-. ' `
25 25 25 25
100 ppb Parathion
Mixture 100 pub HgClg and Parathion
Sap water Control
25 " `
' 25'
25
23 ' 24
18 '
23
. 25 - 25
15 - .l8 ... 12
25 25
On Pebruary 3, the test was repeated using 20 green sunfish in each aquarium but 200 ppb HgCl^- She numbers of survivors were as follows;
DSW 162372 /-
WATER_PCB-SD0000017564
January Progress Report ?age 3
100 ppb EgC-ug
37o. Jish
12 hours 24 hours 3o hours 43 hours 60 hours 72 hours
20
20 20 20 20 20 20
100 ppb Parathion
Juixture 100 ppb TcgClp and Parathion
Pap water Control
20 20
20
20 20 19 15 * 13 10 '
t0o
20 20 ,
20 16 13 .
20 20 20 20 20 20
ih.es e firdings nrovide no evidence of synergistic activity. 0a . ' she contrary, it appears possible that mercury1 may exert a slight antagonistic effect on parathion activity. We plan to repeat these studies using biuegills.
Cn February 2, biuegills were collected from Country Club Lake. Phis fish population is used to stock cages in Choccolocca`Creek. ::r.. fkree aquaria were prepared, one containing 25 ppb parathion, a second containing 100 ppb parathion, and another as a tap water control. Twelve fish were placed in each aquarium for 12 hours/- after which the cholinesterase levels of these fish were determined. Lack sample in this test represents the"brains of two fishes.
2-5 are fish exposed to 100 ppb parathion 6-10 are fish exposed to 25 ppb parathion 11-15 are control fish.
DSW 162373
WATER_PCB-SD0000017565
Tie resnlts of these tests ere shows, in the following table;
Sanple So.
Ootical ':IZ, Density
M ACh nycrolyzed 20' 60'
^Specific Activity
-Average
Percent
Inhibition
2 76-7 ag .OoO
3 SI.4 ag " 76-0 ng
.100 .010
5 >6-5 ag
.025
.S5,
1-36
0.44
0,60
2.35 ' 0.81
4.08
1.16
1.32 1.80
0.39 0.51
72
1 5% --
Or 76.2 ng 7 76.7 Sig 8 . 70.6 Eg 9 77.5 as 10 72.5 ag
.050
.070
.055 .045 .080
'
0.85 1.05 0.90 0.80 l.l6
2.55 3.15 2.70 2.40 3-48
73 , 0.90 0.77 " 0.63 0.99
.82 -
5o -
~ * 83.1 Eg 12 . 8l.4 mg 13 80.0 Eg" 1- 86.2 Eg
V0 p mcr
.145 .120 -It5 .165 195
1.81 1.57 1.81 2.01 2.32
5.43 4.71 5.43 6.03 6.96
1.55 1.34 1.55 1.72 1.9S
I.63
* Specific Ac-civizy ^ K ACh hydroiyzed/hour/ng brain weight
DSW 162374
WATER_PCB-SD0000017566
January Progress'ileport Page 5
On January 25, 1967 vater samples vers collected for gas chrcratograhhic analyses. At the seme time, oluegiHs that had heen caged 2 weeks in Choccolocco Creek upstream and downstream from Anniston were collected for analyses. The findings are
shcnm in the following table (water samples are given in pub;
fish in. ppm):
'
Locality
Sample
Pesticides
'
Choccolocco Creek Bridge 4 miles E. Boiling Springs
ater (1000 ml).
DIE 0.2 ppb (3 unidentified peaks, one very close to aalathion)
Choccolocco Creek Bridge 4 miles E, Boiling Strings
2 Bluegills . (8.58 g).
BBS 0.143 pm (3 unidentified peaks matching those in control fish from the State College papulation).
'
.Anniston Sewage r'~eaiment Plant
Water (1C00 ml).
BBT 1`.7 ppb
'
Parathion. 3.0' ppb
1 -Heptachlor Epoxide %
7 unidentified peaks
... . . " `trace
-
.
Honssnto treatment Pacility Water (1000 ml) (at outfall to" city)
Extremely high background after clean-up on florasil. High EC. . response, even after dilution of the sample, prevented analysis.
Snow Creel; (Promt Bitch
at outfall from lionsento
Plant)
Water (1000 ml)
11 unidentified peaks, several having retention..times'.'similar" to pesticides. -Same of these same peaks reappear in fish caged downstream *
Choccolocco Creek at Eiway 93 Bridge
Water (1000 ml)
BBT 0.3 ppb
_
BBE Trace
'
BBB 0.2 ppb ^
.
Parathion 1.5 ppb
3 unident if ed peaks, one very .
close to methyl parathion. *
Choccolocco Creek at Hiway 93 Bridge
2 Bluegills (8.47 g)
DSW 152375
BBT 0.451 ppm
BBE 0.253 ppm Parathion 0.354 pmn Malathion. ? 4.251 ppm Ten 'unidentified peaks, 5 of which match specific peaks in
Snow Creek.
WATER_PCB-SD0000017567
January Progress ReportPage 6
Chromatographic analyses were done on a kOp Dow-200 column with
a yp Q7-1 nolar column used as a hack-up. In certain cases (Malathion
and Mepxachlor Epoxide) interference was encountered and the identifications
are tenxative. Ihe parathion reported in the hluegill tissues (Hiway 93
Bridge) did not exactly natch the standard and eluted from, the clean-up
column early. However, clear separation was made with the parathion peak
in the xwo water samples reported. Several peaks extracted from Snow
Creek have similar retention times as pesticide standards. Certain-peaks
in Shot Creek appear in blmegills caged downstream. Identification of
these peaks is beyond our present x-apabilities.
.
Conclusions
Tae data reported show no adverse environmental conditions in
Choccolocco Creek or in the Anniston and Monsanto treatment facilities.
Snow Creek continues to be the only environment unfit for aquatic life,
however, there is no evidence that these undesirable effects alter
Choccolocco Creek at downstream sixes. Several materials in Snow Creek
appear xo accumulaxe in fishes caged downstream, but the biological
properxies of these compounds are unknown.
_
vie are acctsnalaxing background data for assessing possible cnolinesxeras e inhibition in caged fish. Survival of caged fish for relatively long periods of time suggests that .the factor or factors ra-.ifo-.g fish Vi' * is in Choccolocco Creek may not be operative at' all times, at least on a lethal level, bhen rains stop and stream-flow decreases, we-hope to study native fishes and determine possible effects of cumulative long-term sublethal factors.
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WATER_PCB-SD0000017568