Document 2jXN8jwR7N0x3EZQXnM20eqQR

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (I-Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC203CF-X. Currentinformationindicatesitisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutyl ether,70.2% water,2.75% Acrylicfoarner,3% sodium octylsulfate1,% triethanolamine2,% sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluoruchomical component of the testsample. METHOD Method: OECD Guideline209 GLP: No Year Completed: 1992 Analyticalmonitoring: Dissolvedoxygen concentrationpsH, and temperature. Statisticalmethods: Resultswere determined by calculatioonfthe % inhibitioin respiratiornate. Test organism source: Activatedsludge mixed liquorcollectedfrom the Metro Wastewater Treatment Plant,St.Paul,MN. Test condition: Dilutionwater: MilliporMeilli-C@wmater Synthetic sewage: per OECD method Reference and testsolution preparation: A stock solutionofthe referencesubstance,3,5-dichlorophenolw,as prepared by dissolving 500 mg in 10 mL 1N NAOH, dilutedto 30 mL withMilliporMeilli-QTM water,then broughttothe pointof incipienptrecipitatiownith IN H2SO4 and dilutedto 1 L withMilli_QTwMater. The pH ofthe reference solutionwas measured to be 7.6. Test substance was added directly totestvessels,initipaHl 6.5. Test vessels: Not given Number of concentrations: one (duplicatedp)lus I reference substance and 2 blank controls Temperature: 18.6 - 21.6 *C Total suspended solids and pH on day of testing:2.6 g/L (TSS in final1.04 g/L)and initiaplH 6.9 and finalpH 7.9. Element Basis: Respirationinhibitioans determined by oxygen consumption. Remarks:Valuescorrectteod20 OCforcalculations. RESULTS Nominal concentrations: 2 Blank controls,referencesubstance and 1000 rng/L(duplicated) Elementvalues: 30-minuteEC50=>1000mg/L 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemigalproportionalone. CONCLUSIONS The testsubstance 3-hour EC50 foractivatedsludge respiratioinnhibitiownas determinedto be >1000 rng/L.The 1000 mg/L treatmentcaused -19% respiratioinnhibitioant 3 hours. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. The studymeets allcriterifaorquality testing.However, the sample puritywas not propedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportion inthe solution. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number K1905-1, 1992. OTHER Last changed: 6/28/00 ENVIRONMENTAL LABORATORY ACTIVATED SWDGE MD(ED UQL)OR Type Test O;L70-'t-5Ac--@i'vek-4-e-->4I- LR k' IqO.'5Dat-e- 1,)l Analyst Page -L of 614-tes-t#-.2c- ACTIVATED SLUDGE MIXED LIQUOR Date Obtained Date Used Mixed Uquor Suspended Solids(MLSS)__,2_, 7_ g/L Adjusted to g/L MLSS in finaltestsolution 9/L InitipaHl &.I_L (lsttestsoin.prepared) F-mW pH "7-el (lastsoin.prepared) Comments TEST SUBSTANCE Sample Description FO -@2rSC F-)( InitiaplH of highest conc. tested Comments Adjustedto pH REFERENCE SUBSTANCE Sample Description 3, InitiaplH of stock solution Comments I-)@e mg/L) Vic 7, Adjustedto pH EXPOSURF- CONDMONS Test Duration 07 .9- /ill-t-ir,<. Test Temperature Range: *c Comments ."4, LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SWDGE RESPIRATION INHSfflONTF-STreOg TEST SOLUTIONS PREPARATION Test Substance: F(@- A03(:! F - X Sample Description: A"60r L iiiL-i) Anilyst: Date: :Aob TestConc. n-gadded MG& 22r 500 mL 5cy cs mL LIQ(I)water mL syn.sewage per500 mL feedR2r 500 mL mL microbial inoculum22r 500 mL CFO Cyr-') 5-N-) TotalVolume mL (i) MifliporLetilli4Rhighpuritywater ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIONTM PAW DATA LR )< #M9 ob Test Substance: Fe --?o3cF-x Source of Activated Sludge Mixed Liquor: A4Lran4It)n<4& -1 ceA-t-cae,24 n+ -Si Pavi , MIJ Date Receiv : /,/,/.;Iz,-Iq x Date Used: /.4 b zq q Analyst: @-/1 -&,i,, A I ;@-f-p- OA@V 30-MINUTE EXPOSURE Test Substance Concentration(t!@/ 0.5 L BK OONTROI- 1 7.9 DISSOLVED OXYGEN CONcem@AMM, mgtl TIME-MINUTES 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 7-b 17-11 -4.a, --10. 1 (aIa (@.0 Temp 5.5 OC ).rl2.L- c-, (o BK CONTROL N I7t,.b-7 bs,!Si;b,3 iG, 1 ,q 0.7 ;71 5.1 q.9lr7 3-HOUR EXPOSURE DLcrzOLVED OXYGEN CONCENTRATIONS. mgrt Test Substance Concentration TIME - MINUTES O.S 1.0 1.5 2.0 2.5 3.0 1 3.S 4.0 4.5 5.0 BK CONTROL I i .@ S, t--7I(e4. -@ea LC-L-6is-Ak 6.9 5.q 6 15. (0.4 LLa- 1 15.9 lsq Temp 5.5 OC 91.0 -i-@% 010.19 BK CONTROL I 6-7 pH of activated sludge mixed liquor: pH of highestconcentmtiontested: Test tomperatu re:, Initial Initi1 a (0,q Final Final (2.5 q .12 EN\ARONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIONTEST #209 CALCULATION OF RESULTS Test Substance: FC.20!3CF-X Analyst:SABMJ 30-Min to Exposure ma/L 1 0.5 1.0 1.5 BKI 7.8- 7.6 7.4 looo.o 3.4-- 3.0 2.7 Iooo.o 3.7 3.3 2.9 DissolvedOxvcon Concentrati s.ma/L 2.0 2.5 3.0 3.5 4.0 4.5 .5.0-1 5.5 TOM, Ic R (1) 4 7-.2 7.0 6.8 6.6 6.4 6.2 6.0 5.7 20.1 0.4109 2.3 2.0--1.6 1.2 0.9 __0.5 - 21.6 0.7190 0 2.5 2.2 1.8 1.5 1.1 0.7 21.3 0.7286 0 .BK2 _7eO .7 6.5 6.3 % diffincontrols(4): 1.8 3-Hour ma/L 12a BKI Expos 0.5 8.4 re 1.0 8.2 -!ooo.o 6.7 6.4 1000.0 7.1 6.9 1.5 -2.0 8.1 7.9 6.2 6.1 6.8 6.6 6.1 , 5.9 5.7 5.4 , 5.2 5.1 4e8 EC50 (95% Cl): JILQQ-BlaL Dissolved Oxycen Concentrati s, mg/L 2.5 3.0 3.5 4.0 4.5 5.0 5.5 7.6 7.5 7.4 7.1 7.0 6.9 6.7 5.9- 5.8 5.6 5.4- 5.3 5.1 5.0 6.4 6.2 6.1 5.9 5.8 5.6 5.3 20.7 TO.4206-1 Rai Tom. -c 18.6 21.0 20.8 R (1) 0.3394 ( 0.3152 ( 0.3442 BK2 7.3 7.1 6.9 6.7 6.6 - 6.4 - 6.: .8 5.6 5-4 1 19.6 1 0.3745 % diffIncontrols(4): 2.9 EC 50 (95% Cl):> 1000 mg/L R (1)R- OWN- WAWO Me 02fL-in) r4 RO-RD*WodioWV- MO.Exi (3)%k'&Nkx RP&CmHWR&Cm)li"O (4)% dnwwm Inowdnft pWki-RaBk2VRSCMIIO(OTwo controlmsust be wkhin IS% of each othw.) (5) kiew adomad FtCordfolef-RaSkl#RaBk2V2 1.072 R&Cmm mom a*ood R.=*role Bk- blar*conbvtsu.mple Tabs- samplelwp Tabs-20*C_______________-j EMARNOMENTAL LABORATORY LRk OECD ACTIVATED SLUDGE RESPIRATION INHMfflON TEST#209 TEST SOLUTIONS PREPARATION Test Substance: Sample Description: Analyst: Date: @,1"2b Stock Solution @reparation:-@.@n exc I@%,@@Iv @ec n m@1-3 RpfeMnco A/4 c@.+, t k -Cv-@ Test Conc. mL added mgd. . per 500 mL gk CepirIo - mL MO(l) water R2r 500 mL 9.pq mL sym sewage feed per 500 mL @& mL microbial inoculump2r 500 mL .2 cl)ln goo TotalVolume - mL (1) mulipore MU114R high puritywater ENVIRONMENTAL LABORATORY OECD ACTIVATED SWDGE RESPIRATION INHIBMON RAW DATA LR t< I?6STESR 8209 Test Substance: ReL(oncip- SourceofActivatSeldudge LiixedLiquor:J4e4r, IU13S4,e- I f'ea mer?i- at+ MAI DateReceiv DateUsed: Analyst: 30-MINUTE E)(POSURE Test Substance Concentratio(nf@5/zo.s BK OONTROL 1 7 '4.q DWMVED OXYGEN COMe4TRATIONS. aXyL TIME-MINUTES i.o 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 7. 7. 9 Li Temp s.s Oc BKCWTFXXI s. LiS 3-HOUR EXPOSURE Test Substance Concentrationo O.s i.o SK CONTFIOL I g.q DWMV ED OXYGEN CONCBffRATUV-.,, mgt TIME - MINUTES Temp 1.5 2.0 2.5 3.0 1 3.5 4.0 4.S 5.0 5.5 OC 7-cf.7.1,-7-.@57.q 7-1 -7,0 to.q (o-7 lig,b '4 q ;@0.-7 BK CONTRM I pH of activatedsludg r: pH of highestconcent@alloltiestecl: Test temperature: I lnitial, Initial7,0 Final Final .--7. EWRONMe4TAL LABORATORY OECD ACNVATED SWDGE RESPIRATION INHIBITIONTEST #209 CALCULATION OF RESULTS Lr: Date: Test Substance: 35-DICHLOROPHENO 'AnalystS:AMYI 30-Minute Ex osure DissolvedOxyc en Concentrati s, mq MQ /L '0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 1 5.0 5.5 Twm. -C =OEM= R (1) Ra (2) SKI 7.8 7.6 7.4 7.2 7.0 6.8 6.6 6.4 6.2 6.0 5.7 20.1 0.4109 0.40811 10 7.8 @-7.5 7.5 7.3 7.2 7.0 6.9 6.8 6.6 6.5 6.3 20.8 0.2739 0.2591 B 7.0--l 6.7 1 6.5 % diffIncontrols(4): 1.8 3-Hour EXDOS r m /L 0.55 1.0 1.5 SKI 80-.4--, 8.2 8.1 10 8.1 8.0 8.0 6.3 6.1 5.9 5.7 5.4 5.2 1 5.1 4.8 EC50 (95% Cl): DissolvedOxyc en Concentrati s,mg/L 2.0 2.,56 1 3.0 3.5 4.0 4.5 5.0 5.5 7.9 7t7.6 7.5 7.4 7.1 7.0 6.9 6.7 7.9 7.8 7.7 7.6 7.6 7.5 7.4 7.3 20.7 TOM. Ic 18.6 20.7 4206 10.40061 RaCm (5): R (1) Ra (2) 0.3394 0.3741 0.1600 0.1524 7.3 7.1 6.9 6.7 6.6 6.4 6.2 6.0 5.8 5.6 5.4 diffIncontrols(4): 2.9 EC 50 (95% Cl): 19.6 1 0.3745 0.3851 RaCm (5): o-now wmko ads (ffoon -Ron) o. 1.072 M,Ram_R to2MmIM.EX] R&Cin. moon adbmod R,amrols M %k*mmg"Racm@,WROCOQIIOO SksblmckontSr-oSl&.nV4 (4)% dnwomIn6w**IPLPAU14WUVFtsCm()T1wm0on0lromkLibMewkhi1n5%oloaholtw.)Tabsu-mplIeWM 7-'(5M)om adiLPO1W%conif0fItRGUI*RobtM x. Tobs-20*C 7- 1 4 c 0 a P-GIR A T I ol RA@ti OF,,IVALITY Addrm Sa"W By Date Report To: BifiTo: P.O.0 ENIIirRiegferarice ProledNaffie No. PRESER LU W REOUEST w w CHAIN-0 AnaW[ml Pace CMM No. Pace pmjw Paoe ProjW No 'Floc.,,SsWI)m SEE REVERSE SIDE FOR INSTRUCTIONS