Document 2jD3GxMvdLBjorEykdmEJqbB6
ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA)
T TESTE SUBSS TANCT E Sue0S 00TAN 00GE Identity: rPeefrefrlrueodrtooocatsanPeFsuOlSfAoAny,laFmCi-do1(2e8thoyrlaascetthaetem;ajmoarycaolmspoobneent of
[F(Ch-e1p2ta7d,ecFaCf-l1u2or8o,ocFtCy-l1)s0u9l,foonrylFlC--,1p0o9t-aXs.si(uGmlyscailnt,e,CNA-Set#hy2l-9N9-151-7)
Remarks: laboratory
as
The test sample is E2566-2. Current
FC-109-X referred to information indicates
by the test itis a mixture
of
18-
223%%ettheasntoslu,basntadn2ce-,6%61C%-waCteflru,or1o2al%ky2l-cmaertbhooxxyylmaettehcyolemtphoouxnydpsr.opaTensotl,
sample was from 3M production lot 2.
mTihxetfuorleloofwitnhgestuesmtmsaurbystiasnacbebrinevaiwaatteedrb/eocrgaaunsiectshoelvteesnttssaolmuptlioen.is a
Data may not accurately the test substance.
relate
toxicity
of
the
test
sample
with
that
of
METHOD:
Methods: OECD 202 Test type: Static acute GLP: Yes Year Completed: 1988 Species: Daphnia magna Analytical monitoring: Temperature, pH, and DO
RESULTS
Nominal Element
concentrations: Bk control, 1.0, 1.8,3.2, 5.6, 10, values: 24-hour LCso = >32 mg (estimated)
18,
32
mg/L
24-hour NOEL = 32 mg/L
48-hour LCso = 1.5 (1.0-1.8) mg/L
48-hour NOEL = 1.0 mg/L
All element values based on nominal concentrations.
dReesmcarrikbesd: iTnetshteintgeswt assubcsotnadnucceterdemoanrktshefimelidx. tuTrheeofvatlheuetersetpsorutbesdtaanppcleiaess dtoettehartmimniexttuhreeiamnpdacntotofthtehetepsrtessuebnscteanocfet.heNoorgaatntiecmpsotlwvaenstsmoardwehatot portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethylacetate.
000255
cC0 oOnNeCwL0 uUsSiIoO0 NnSs 000000000
`mTgh/eLFwCi-t1h0a9-9X54%8-choonufridLeCncsefionrteDravpalhnoifa1.m0a-1g.n8amgw/aLs. dTehteer4m8i-nheodutronboe 1.5 observed effect level was 1.0 mg/L.
PSauublm,itMtienrn:es3ot1a,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St.
bA0 DAtTAAQ0 QuUAALb0 ItTY 0 ~~000000000 tReesltiianbgi,libtuyt: laKcikismiasncahlyrtainckalincgon2f.irTmeasttiionngomfeteetstssalulbcsrtiatenrciaefcoornqcueanltirtaytions. Theres a lackofcharacterization of the test sample.
RREEFFEERRENCCEESS
"This study Columbia,
was conducted Missouri at the
raetqAuneasltytoifctahleB3ioM-CChoemmpisatnryy.Laboratories,
Inc.
of
oOmTOHERR
Last changed: 5/18/00
000256
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: - ESTCOPY AVAILABLE
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SPONSOR
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Acc5a:
se.3MMoCnoasspeadnyElnabaravy PC Enviromental Labs
Butlding 21-26-05, 500 Bush Avenue
E [
Sc. Paul, Minnesota 35106
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sty Tine
Acute ToxDtaepthenyiaofsaga2s566-2 to
;
Data esquisevsy
OECD Cuideline 202
k
pi
Aumion
5
BScooltotgtFersazs11er
sctuomiereonx
January 9, 1988
:
PERFORMING LasoRATORY
Analycic`aAlquBal7t2ioc0-0ChTeoBaaxsttscaotlAroByEgyLaLabDnoiervaitsoiroines, Inc.
ColumbiPa.,0.WiBsosxou1r0s37 65305
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8c LABORATORY PROJECT TD os616
Foc. tot ug
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000257
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Subsicced By: A7n20a60l.ytFiaBcvoeaxl8dBocisoL-aCnheentscry Laboratories, Tne.
-
Cotusia, biasourt 65205
ag
(314) 474-8579
Prepared 35:
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000258
ABC LBS 436616-2 of 4
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BEST COPY AVAILABLE
:
TABLE OF CONTENTS
-
Page No,
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ISETEBATE ACCT TOXICITY COPENDINN, + + + - + +. 26010
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000259
A5C LABS 93661623 of 48
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Table No.
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1 CS5heAa8tcc"asl CAqhuaartaiccterTiosntiiccoslogofy DWeilvlisiWoantser + Us+eod + + + + 3616-10 2 cure Tomteiey of 55662 co Daphnia Zama. + . . 3616-11
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ASC LABS 436616-4 of 48
PROJECT PERSOMSEL
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followin: ABC personnel assisted with various phases of the stuly,
;
Nose
Teele
Inttiale
Michael Ruddy
Blologtcal Techntctan IX
fe
8
Scott Frazier
Biologist IT
SF
Setlrey Lathan
Bologteal Techntetan 1
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Heat
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ABC LASS #36616-5 of 46
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INVERTEBRATE ACUTE TOXICITY CONPERDIU
--
Subject: "AABcCuteStuTdoyxteNuistbyerof 36E621566.6-2 to Daphnia magna,"
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Sponsor: 3M Company
h
Tewcing Facilieys AunaaltyteicaTloxiBclool-oCgheystDsitvriysioLnaboratories. Inc CPoot.uabBioax, 1M0i97ssourl 65205 (21niTanss
Location of Origiral Rov Data and Final Report: BE3E4utClodmiPpnCagnEyn2v1i-2r6o-n0s5e,nta9l00LaBbussh Averine St. Paul, Wimesots
Test Materfal: 25662
Test Concentrations: Control, 1.0, 1.8, 3.2, 5.6, 10, 1% and 32 mg/l.
Dilucton ater:
246 mg/L (hardness), 178 mp/) (alkalinity). 5.0 (pi).
Test Dates: Intciation - Jawary 7, 1988
Terstnation = January 9, 1988
E
Lengeh of Study: 48 hours
E
Results: 24-hour Legg = >32 m/1 (estizated)
ZU-houe NOEL = 3238/1 48-hour Legg = 1.5 mg/L (95% C.T. = 1.0-1.8 mg/L)
:
48-hour NOEL = 1.0.38/1
Test Spectes: Daphnia Zagna Source of Orgontuss: ABC Laboratories in-house culture. Are of Crgantens at Study Intetacion: First-instar (24 hours old)
e262
AIC LABS 36610-6 of 48
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suouny
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AC LABS 43681607 of 48
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0002455
ABC LABS 936616-9 of 48
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3
Temperature Dissolved Oxygen
oHHardness (Cac0,)
Alikalinicy (ac0,) Conductivity
Total Organic Carbon Suspended Solids
BESTCOPY AVAILABLE f
17 = 220%
7.6 = 9.1 ppa
7.6 - 8.4%
E
206 = 275 ppa
i"
224 = 336 ppe
500 - 650 ushos/ca
1.4 pp (0.9 = 2.8 ppm)?
0-13 ope
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Zine Measured organophosphorus
2 ppb
After aeration represents seasonal variation, difference from ome month to
Caverage of all well vater values from January-September, 1987 "Total conthly range of organic carbon
000256
ABC LAYS 936616-10 of 48
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1650 (R/D)
2 hours 32 (estimated)
5
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"teassay concucced at 20C (2.0).
$952 confidence Listes could not be entablished.
937 confidence Listes.
Trheespe2ct4ivealnyd, dbba-sheoduron nho-eefafbeacetnceconofcenstorrattailointss avnedresbn3o2rsaanldcf1f.e0ctsm:g/l,
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0002757
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AKC LABS 936616-11 of 48
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ARC LABS 3616-13 of 18
QUALITY Assumasce stares
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ABC LABS 436516115 of 48
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APPENDIX = RAW DATA
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ACTIVATED SLUDGE RESPIRATION INHIBITION I TESTE SUBSS TANT CE S~U ~B ~ ST=A0N 0 CE Identity: Perfluorooctanesulfonylamido(ethyl)acetate;mayalso be [Fr(eChf-ee1pr2tr7aed,detcFoaCfa-is1u2oP9r,FooOFcStCyA-l1A)0,s9u,fFoCon-ry1lFl2C-8,-o1pr0o9atsXas.tshie(uG`mlmyascjailontr,e,cCoNAm-Speto#hnye2ln-9tN9-o1f 517)
iRteismaarmkisx:ture Tofhe19te-s2t3s%atmepsltesuisbsFtCa-n1c0e9,-X6.1%Cuwrarleenrt, i1n2fo%rm2a-tion indicates cmaertbhooxxyylmaettehycloemtphoouxnydpsr.opaTneoslt,s2am%pleethwanaosl,fraonmd 32M-6p%roCd.u-cCt;iofnluloorto2a.lkyl
The following mixtureofthe
steusmtmsaurbsytiasnacbebirnevaiwaatteedrb/eocrgaaunsiec
the test solvent
ssaolmuptlioeni.s
a
tDhaettaesmtasyunbosttaanccceu.rately relate toxicity of the test sample with that of
vMEeTHoOD:: =
Methods: OECD 209
GTeLsPt:tyNpoe: Static acute
Year Completed: 1987
Inoculum: Activated sludge mixed liquor collected from the CityofSt.
Paul, Minnesota Wastewater TreatmentFacility.
IInnooccuulluumm hmoilxdeidngl:iqNuoornseu--spuesneddetdhesdolaiydsit:wa2.s9gobLtained
EAnxaploystiucrael pmeorniiotdo:ri3n0g-:miDniustseoslavendd o3x-yhgoeunrsconcentrations
Measurements: Dissolved hours of exposure.
oxygen
depletion
rate
after
30-minutes
and
3-
RRESSUWLTSS
mNgolmLinal concentrations: 2 Blank controls, 100, 180, 320, 560, 1000 Element values: 33-0h-omuirnuEtCesoEC=s>1=0>0100g00img(/5L.9% inhibition at 1000
mg/L)
All element values based on nominal concentrations.
0Co273
uCpotnatkreolrarteesspwoansse4:.3T%h;ethluarsgetshte ptewrocceonnttdrioflfsewreenrceewbietthiwnee1n5%conotfreolach other. 1R0efmegr/eLnciendsuucbesdt4a5nc%einrheisbuilttiso:n i3n,r5e-sdpiicrhaitoiornoprhaetne.olTahtisa wcaonscewnitthriantitohneof normal range seen in this laboratory with this inoculum.
dReesmcarrikbesd: iTnetshteintgeswt assubcsotnadnucceterdemoanrktshefimeildx.tuTrheoefvtahleuetersetposrutbesdtaapnpcleieass dtoettehartmimniexttuhreeiamnpdacntootftthehetepsrtessuebnscteaoncfe.theNoorgaatntiecmpsotlwveanstsmaordwehtaot Ppoerrtfilounoorfotochteanteosxuicliftoynyclaanmibdeo(ceotnhtyrlijbaucteetdattoe.the
cCoOnNcCtLuUsSIiOoNnSs
000 0000 00000000000
The FC-109-X determined to
3-hour ECs for be >1000 mg/L.
activated
sludge
respiration
inhibition
was
PSauublm,itMtienrn:es3oMta,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St.
p0 DaATtAaQuUAaLrITyY
tReesltiianbgi,libtuyt: lKacikmsiasncahlyrtainckalincgon2f.irTmeasttiionngofmteeesttssalulbcsrtiatenrciaefcoorncqueanltirtaytions. wThaesrteesistead,laccakreofmcuhsatrabcteertiazkaetniownhoefntihnetteersptrestaimngplteh.e rBeseuclatsu.se a mixture
R0 REEFFEERR0 EENCEE0 SS ~ 0~ 0000000
This study was conducted St Paul, MN, Lab Request
bnyumtbheer3ME2C1o13m.pany
Environmental
Laboratory,
O0 OTTHHEE0 R 00000000000 Last changed: 5/18/00
000274
So
20
REST COPY AVAILABLE ENVIROMENTAL LaRORATORY
ACTIVATED SLUDGE MIXED LIQUOR
oni a2 in
Tive tes 00 Agvivated Slodae Respitation Tubibunon # dod
SourceMgice Lonete Tezatment lane $4 Dau Mu
Date Obtained JO: 30-87
Date Used__J 36-57
i
Mixed Liquor Suspended Solids 055) 2.9 g/L Adjuscsent bons s/t
Inne 79 Ful:
coments,
1
|B
pa 1
E
b
3
Test substance SempleDescripeton E129:K (41: Ae sovi-c Let 3) FE SurGarkant Ssople piso con nly: 2.3 Mfastasns Apne
Conments_
-
a
Reference Subseance
Sample Description 3 4.
Sesple gh 2.0
cco phenol
djustaenc_ Alone
coments Sinck Soliiion Seo 2 le
% Exposure Conditions
Sr
Test Duration __3- Haves
t
Test Temperatur, 19-20 %
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DWIRONENTAL LABORKTORY ACTIVATED SURGE RESPIRATION INHIBITION TEST #200
EC209:(edi`cCAiLGzNcATIuONrOeF tRESoULaTS) PC
--
=
. A w E23
Datefp:30787
Sunchad AnalyCstA"3SYI3A
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Concentration (mg/L)
[osvel Josue | -- Oxygen Uptake Rate R
R Corrected!) to 2c
* Inhibition in, RespirationRate(2)
S
5
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Ce [ ews c iys i e TTTceo a c uasu aen e ncs
=
T= y
--Bkclo [|co osi. gs5| T2 esd aaa)] n|ga 33
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RN
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000278 "Se Seeleetpieyn epAireeryWS
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OECD ACTIVATFO SLUDGE RESPIRATION INHIBITION TEST 1209
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RAN DATA
pSToesustrcepSbosfteAacntcivea:tL_iesi3d.S5l2udDguecMhixleedLcieqpuhoern:sAl0Q0oe0tCeeicUnoseandtc:ee[TS0reu-ob3tc0mo-tn2as7nPeleier GDL]ad [E
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EAT ACTIVATED SUIGE RESPIRATION INIIBITION
3.6= Ducllocaphenel ReCrene
TEST #209
Toucant
ig Date Lo:30
R43 os
3f
30 Mie Esme
-
ConcTeensttraStuibosntance Tog) Oxygeneen UpTptke akeRatfaree || AR CSorEpected) et n ianre TT
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RtCaoreyected(t + RIenshpiibriatitoinon Rinace
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3DiterelAcn,LcR"Ce)dzy 100
@ 8 hitfamd + RC), R x im
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7
6TR1o=o.p0E 87j,enLxup=tak1p e,0r5a5te e- a8f0c0y/L-nin.)
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VAILABLE
HR
000231
1
IB 1
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: 5 -
RR
MICROBICS MICROTOX TOXICITY TEST
ITEESSTTSSUUBBSSTTAANNCCEE~~~ 00000 0 Identity: rPeefrefrlrueodrotoocatsanPesFuOlSfoAnAy,iamFiCd-o1(2e8thoyrllaascetthaetem;ajmoarycoalmspoobneent of
[F(Ch-e1p2ta7d,ecFaCf-l1u2or9o,ocFtCy-l)1s0u8l,foonrylF}C--,1p0o9t-aXs.si(uGmlyscailnt,e,CNA-Set#hy2l-9N9-151.7)
Remarks: itis a mixture
Tofhe19te-s2t3s%amtepsltesuisbsFtCa-n1c0e9,-X6.1%Cuwrarteenrt,
information 12% 2-
indicates
cmaertbhooxxyylmaettehycloemtphoouxnydpsr.opaTneoslt,s2am%pleethwanaosl,fraonmd32M-6p%roCd.uc-tCiofnluloorto2a.lkyl
The following mixtureofthe
stuesmtmsaurbsytiasnacbebirnevaiwaatteedrb/eocragaunsiec
thetest solvent
sample is solution.
a
Data may not accurately thetest substance.
relate
toxicityofthe
test
sample
with
that
of
Me MEETTHOODD: ee ---- TMeestthotdy:pe:MiSctartoibcicascuMtiecrotox "BASIC" Procedure GLP: No Year Completed: 1987 ASnpaelcyiteisc:alPhmootnoibtaocrtiengr:iuNmopnheosphoreum lSitnaetairstriecgarlesmseitohnopdrso:grECazmn,prEovCisd,edanfodr EMiCcsrovbaiclsueMsiccarloctuolxatesdysbtyesmt.atistical TTeesstt coorngdaintiisonms:soAurscpee:rMMiiccrroobbiiccssMMiiccrroottooxxmreetahgoednt Replicates: Two
eReEUSUVLETS
ENloemmiennatlvcaolnuec:entrat5i-omnisn:utBelaEnCkzcoo=ntr1o7l0,
125, 250, mg/L
500,
1000
mg/L
S-minute 5-minute
ECs ECan
= =
650 (560-750) >1000 mg/L
mg/L.
15-minute ECzo = 140 mg/L
15-minute 15-minute
ECs EC
= =
410 (320-530) >1000 mg/L
mg/L
All element values based on nominal concentrations.
000282
dReesmcarrikbesd: iTnetshteintgeswtassubcsotnadnucceterdemoanrktshefimeidx.tuTrheeofvatlheuetersetpsorutbesdtaanppcleieass dtoettehratmimniexttuhree iamnpdacntootftthheetepsrtessuebnscteaoncfe.theNoorgaatntiecmspotlwveanstsmaordwehtaot pPoerrtfilounoorfootchteanteosxuicliftoyncylaanmibdeo(ceotnhtyrlilbaucteetdattoe.the
CCoOnNcClLuUSsIiOoNnSs
00 000
The FC-108-X 15-minute ECso for Photobacterium phosphoreum was
determined
mglL.
to
be
410
mg/L
with
a
95%
Confidence
Interval
of320-530
PSauublm,itMtienrn:es3otMa,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St.
RDAATTAQQUUAILITTYY eeeeee tReesltiianbgi,libtuyt: laKcikmsiasncahlyrtainckailncgon2f.irTmeasttiionngmoefettesstaslulbcsrtiatenrciaefcoornqcueanlriatytions.
There is a lackofcharacterizationof the testsample.
RREEFFEERREENNCES
"STthiPsauslt,udMyNw,aLsacboRneduqcuteesdt bnyumtbheer3ME2C1o13m.pany Environmental Laboratory,
om0 OTWHeERR 000000
Last changed: 5/18/00
000233
h
*
--
23
3
Toxicity of FC-109% (wdici-cui-o tora) SAB
To Muirobial Systems
BESTCOPY AVAILABLE gm
Miccotox
Test Conc. Peccens Liga Cos
:
malt a:
ew op
125
Rl
as
i
350
25
ag
=
Sco
Hi
48
loco
3
79
Tee Te TTT TT
sna| (sears)
(33%530)
1
Eg
Jlcco
210c0
Eb
OECDrmAchuawd PerScludegentResTpimatiobnriTaanmsin
po
Som
ae
leo
-
5.9
180
-
2
3a0
-
[3]
Sto
a0
3.2
loo
-
54
Test (acd)
s Eero ery 1000
> toco
:
0002534
= RR
a
BEST COPY AVAILABLELE ccs wiceLrAoBOsRAsTtORoYn
a ATA
wian3z IH
pgd of 2
I
Test sussTace: ( FC o8 100-1 X -26cLei b 3)- FEo Scu hond : -0
Test oaTE: [0-15 SE
po
wast: Anos G6 Beach
3of soe are
ep le
8
Bo
0
3
BLANK CONTROL
BUPLICATE
12s
Duplicate
TLmuoOG|| sASn. TO| Cd[GR sa E1oR | iO nTRS
gc | ve | 55
4
BH
ad 2 [2]
qq | eA | He
Joo ba qo
A
3
a 3
a
[3]
ase
a
Duplicate
q7
7
Hy
a | 51 |
a
see
o
Dupliat
a4 43 3
jo || 31
5
1oco
41
2'
!2
os
Duplicate
44 28 +3
Ter Sovmaniet | Swev Selurien, $00 ng asond OF weir. ack mlzamy
Cleon tiguid) geAmtRieSnrTaelntl(y12mSisp4,3cirblaea.dyywuieartrehdhwdavteeer.pchoSnteeG7c.kw3esiorvleudh,iWciTn eohnCasmfCaHiint eoter, E]
=
EAI Io tn C8rAaenete Vnyd pr eaerddyesSoahmze,67
Diluents Mictetis 2% atl Soletien
0NORS8S
Diluttens I InnineBalecacnAcee entrantdee nn s uate da ewT ble thIe Gin assayE Cerrar
OF water vod! Distined 19-87, aerahien started coms, Pl b-L
J
:
:
o~
f 8]
-
JEST[EST CCOPYHP AVAILABLE conic croc sr
iweEAdNarZaaz
sae riose BL
FC-HilcoRodtoxx DdArTA-aREtDUoCrTIoONume FC Surfactant
rd),MaslyuePeSAdERe RHRs
[RE
bE
fTrrSleme Teeor Tormsaek Crishousld sEFeepWirthien t0-0t7.focsB----F |
4
WEAN BLE Batis = 3a nos 0 we we
e seyT 1 1
worLIzED Liou ass (ervecr) ean dIleonk Batsleux lal ample testing
.!
SeEit TCoAncentrA ation |G 217 PSJS SNS as=a: AS SO
CPLS |
B: e Tim C98 1 PN D; 7o125o wale|oo0a.a22nn9
oeE.E3]m35 [00]
3
i]
in
ua vss a) =pt F x 10
5
FPLE cuvertE
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Bf5est2Co5ncentration
THT
ig
i
TER TE
EX5)2
oT Ta
I
Baw OT5 1= | BBeoo 2 1&5 --T1T--1] Jeoo
| 2 is
|5
4
[1
EFPICTIVE ConcENTIATIONS een le (oan using "MC" data pafrs.)
SL wes
so [ [o wem [| en |
4
EN Tre ETrTeeERS
3 (ai=szad|
|
i
5 > 1000
COZ
iE
