Document 2j655gmw35aZr8KmKOjOddV2g

A R . 2 6 - O S Z Z ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH) TEST SUBSTANCE___________________________________________ ____ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-780B. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 788-20. The test sample is FC-780B. Current information indicates it is a mixture of 0.58% PFOS, 14% diethylene glycol monobutyl ether, 76.7% water, 5.5% urea, 1.17% Hydroxy foamer, 1.15% sodium octyl sulfate, 0.8% polyoxyethylene monooctyl phenyl ether, 0.05% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity o f the fluorochemical component of the test sample. METHOD:_____________________________________ _______ Method: Methods for Acute Toxicity tests with Fish, Macroinvertebrates, and Amphibians, E PA -660/3-75-009,1975 Type: Acute static GLP: No Year completed: 1978 Species: Lepomis macrochirus Supplier: Dale Fattig Fish Farm, Brady, NE. Analytical monitoring: Dissolved oxygen, pH, and temperature. Exposure period: 96-hours Statistical methods: LC50 values and 95% confidence intervals were calculated using the U.S. EPA (Duluth) Probit computer program. Test fish age: Juveniles Length and weight: Average length = 45 mm Average weight = 0.92 g (wet) Loading: 0.57 g/L Pretreatment: None. Test conditions: Dilution water: Carbon filtered well water, St. Paul, MN Dilution water chemistry: Hardness: 232 mg/L as CaCC>3 Alkalinity: 220 mg/L as CaC0 3 Conductivity: 405 pmhos/cm Lighting: A daily photoperiod of 16 hours light and 8 hours dark with a 30 minute transition period was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. 002545 Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: All glass aquaria (35x20x20 cm) containing 16 L of test solution. Number of replicates: 2 Number offish per replicate: 10 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.7 - 8.0 (control exposure) 7.6 - 7.9 (1800 mg/L exposure)* Temperature range (0-96 hours): 18-18 C Dissolved oxygen range (0-96 hours): 5 . 2 - 1 0 mg/L (control exposure) 0 . 1 - 1 0 mg/L (1800 mg/L exposure/ * 1800 mg/L (intermediate concentration) exposure values used because the highest two concentrations resulted in total mortality by 48 hours. RESULTS_________________________________________________________ Nominal concentrations: Blank control, 1000,1350,1800, 2400, and 3200 mg/L. Element values: 96-hour LC50 = 1500 (1300 -1 7 0 0 ) mg/L 96-hour LC5o = 1600 (1400 -1 8 0 0 ) mg/L (replicate) Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS___________________________________________________ The test sample 96-hour LC50 for bluegill sunfish was determined to be 1500 mg/L with a 95% confidence interval of 1300 to 1700 mg/L for one replicate, and 1600 mg/L with a 95% confidence interval of 1400 to 1800 mg/L for the other replicate. Remarks: Low DO levels in the first 48 hours (<60% saturation) could have initially impacted the organisms. Even though it did increase slightly in the next 48 hours, it was still <40% saturated by 96 hours. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 002546 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. The study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________ ________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1978. OTHER_____________________________ :____ ,___________ Last changed: 6/28/00 002547 313 j* io r m 674711a TECHNICAL REPORT SUMMARY TO a ONaot vember 15, ' 197 TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN pportant - If report sp rinted on both sides o f paper, send tw o copies to TCC.) Division Protect Haport Title To A u th o rit) Environmental Laboratory (EE 8 PC) Commercial Chemicals ^_FC 7^80B^/(lot 788-20): Results of Aquatic 96 Hr. D. L. Bacon .'} <Y JtTi* A. N. Welter "U n Tests Dapt. Number 0535 Protect Number 9970012600 Report Number 004 employ Numbsnu V 09362 S E C U R ITY Q Opart Closed (Company Confidential) (Special Authorization) 3M C H E M IC A L w R E G IS T R Y r New Chamicali Raportad O Taa 0 No KEYWORDS: (Salaet tarm t from 3M Thesaurus. Suggest other applicable tarm t.) EE $ PC - Div. (EnvironmentalLab) Toxicity (Aquatic) CURRENT OBJECTIVE: To determine the acute LC5Q value for the bluegill sunfish (Lepomta maarochirua) exposed to varying concentrations of FC-780B. R ETO R T A B STRACT: (20 0 -2 5 0 words) This abstract inform ation is distributed by the Technical Com m unications Canter to alert 3M*ers to Company R & D . It is Company confidential m aterial. The acute static 96 h LCS0 for bluegill sunfish (epomis maorochirua) exposed to varying concentrations of FC-780B ranged from 1.5 to 1.6 g/1. Information Liaison Initial*: '3.M CO>,NFID*Ev.NT- IA L-h 002548 ''{*>. 2 CONCLUSIONS In a replicated static 96-hour LC__ aquatic test, the calculated Iso values for bluegill sunfish [Eepomie m aarodiirua) exposed to FC 780 B 788-20 were 1.5 and 1.6 g/1, respectively. Materials possessing an LC5Q of this order of magnitude are considered to present an insignificant hazard to the aquatic environment. INTRODUCTION The test substance FC 780B (788-20) is a yellow minimally viscous fluid. Tests performed on this material evaluated its aquatic toxicity and the data obtained form the basis of this report. '' ' : . - ''' METHODS '/ .-V - A. Aquatic Testing The test protocol utilized .for this study was modeled after that described by USEPA (1975). ACCLIMATION PROCEDURE The bluegill sunfish (Lepomie macroch iru s) were obtained from a private hatchery.* Stock fish were held in a fiberglass holding tank filled with carbon-filtered well water at 14-15C. A daily photoperiod of 16 hours light and 8 hours dark with a 30 minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min'daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated for 14 days prior to testing. An acute shoTt-term (96-hour) static bioassay was performed on FC780B. Carbon-filtered well water of known composition wasused as the diluent (Attachment 1). All glass aquaria, (35 x 20 x 20 cm), containing 16 1 of diluent or diluent plus toxicant o m ^ i s ^ aV'^V-'study chamber. Ten bluegills, uniform in size were tested at each chemical concentration. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. Bluegill experiments were conducted at 18C. Mortality, temperature, dissolved oxygen level and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations were recorded relative to swimming pattern and behavioral changes induced by exposure to the test chemical. * Dale Fattig Fish Farm, Brady, Nebraska Commercial Fish Food of known composition 002549 'I11 1 3 These studies were performed in replicate using the following FC 780 B (lot 788-20) concentrations: 1.0, 1.35, 1.8, 2.4 and 3.2 g/1. L C ^ values with 95% confidence limits were calculated using the US/EPA50 (Duluth) Probit Computer program. Organisms used in this study were considered to be generally healthy and free of disease. RESULTS The bluegill sunfish (Lepomie macrochxvue)used in this study had an average weight of 0.92 +_ 0.36 g and length of 4.5 +_ 0.58 cm. The loading factor for these studies was approximately 0.57 g/1, below the maximum regommended loading factor of 0.8 g/1 (1). Temperature was maintained at 18C, while pH ranged from 7.6 to 8.0 pH units throughout this study. Dissolved oxygen values decreased sharply 48 hours post initiation of this study. Test aquaria solutions were originally clear, becoming cloudy on day 2, an observation which coincided with low D.O. levels ( 0.1-0.5 mg/1). As the test solution cleared the D.O. levels increased. These abnormally low D.O. levels did not contribute to an increased mortality. This is considered to be presumptive evidence that the calculated LC_Q values are accurate reflections of test chemical induced mortality, per se. In this replicated study the 96-hour LC5Q values were 1.5, range 1.3-1.7 and 1.6, range 1.4-1.8 g/1 respectively. These values translate to LCsq values of 1500 and 1600 ppm. Chemicals possessing an LCg0 value^in this range are considered to be an insignificant hazard1* to the aquatic environment1 REFERENCES (1) US/EPA Methods for Acute Toxicity Test with Fish, Macro invertebrates and Amphibians, Ecological Research Series 660/3-75-009, 1975. (2) Registry of Toxic Effects of Chemical Substances, * 7 6 Edition, Christensen, H. E. and E. J. Fairchild, Eds. p. XV and Ci, U. S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, D;C. June, 1976. 002550 -- --TS m r t r -- -- ----- -- ----- -- *W4K* .. ...... .................... Tt ..iIii7T) \ s r .. - - z iav iivaV A r im \$ z o i> o t ; Al 11 M 1 .... . ..... ji .. . . V 11 l Ai t 1 V "ti j .__ t ___ i i i U L iU M W m Q U Z J R E I K J C U ------------------- - t r ~ * -----------1" n L I B J I fcUJ' ju J D lB E J| ru n tiii n ^Lu3W .il U K la JH n iK ^ KffiQCS B U B3I 1V 1 : m% w ' U ' A 1 t DL - * . T L ili U J 2 kiJLI C 7 J U aiC J ............ 1'i - /# " U i t-f-i i "i . ... un mj i ln .iinu vr Mi K iJri tniTf Ti CTL. JHBlal B m ........... I h t - f " . I I t f M n m .u *iT i cnKJjtai C 9 3 i---J L _ i & g f c ^ g j y j i l j i b i j i nza TTi M tm * i i 1 1 I LN3{JVLLV niiST *--i m : |4 il4 < ?S 3 ( -ndWV5 m u imkjK T 9 E ( 'ON \n u rr MDl.lMriVIAUt-K' HNVIRONMLNTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SHEET lypcj ___Material Tested /'c /6 n 74 Lot v-u /yy . 'st Organ i s * A v g . Wt. , i I c) r . .) f i t , Vendor A..-/- grams Avg. Size y.y i. Date Rec'd^/jy/yj Batch No T i t o ere. Test Solution Volume_____ Lk______Liters/Tank No. Organisms per dose /p Time Started l i - J ' o Date Started IC 'jto h S _______ Date Completed jo U H h i Dilution Water Carbon filtered well (Temp. % C ,, D.O. 7 -} water Investi gator LC SO (9S% Confidence Limits) 2 _ (Full Signature) JiBtE* P* /* 7 7 ) /*)* & Cone. mg/1. Total Vmount Added Control /o fI/ /* . 7V ( _ l ______ li J/. Y li 7'c i , T e s t Temp . C i g n a t u r e f Date jC o m m e n t s : ?4:llour s Total D.O. No. Dead . PP 48-1lours 72-Hour S Total D.O. T otal D.O. No.Dead P p PP No.Dead pH PP . J* 0 71 jL L ,, 0M a 7-7 < r f 0 7? I d <? 7 * V-i C dA il L/ w r . < 0 __________ O Q & 14 8.0 .AW 7.7 -.if 74 1 ?it -- 1 1 ~~~ 1I * H V u J A tU j P t n h t f ( W jJ M * to t <>fi & s < m , i jI m iJ L ^ O 1 1 /. 7 Vi a 1 11 1 -- 19' 9 6 - -Hours T o ta l D.O No.Dead ppm O 9. $ 9 1H n li 9 M .l _-- E f f e c t s o t h e r t h a n M ortality C one. J Sym ptom s or Reactions m g/1 B E S T C O P Y AVAILAB LE __________ _______ _______ / b C S f r -------------....... -......... :----- ------------ ---- :- 24-Hr. T otal No. A ffected 48-Hr. 72-Hr. 96-Hr. C on trol /O /Ja" i'd v/ ih i _ | i j ^ - - i u r e Date Comments : -*.g 7------------------ 00 O 1 o *0 7 1 9 --_ -- \otr?.y. M ( i m 0 1 .... ! A N M U X *. T^ e ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SHEET Cj r Material Tested f /y ri Ara - Lot ' y / S J 1 t' 1est 0rKunisra Vendor Avg. Wt. * ( l ct ' , & ' ti grams Avg. Si ze V-f T j d 0ate Rec'd 'Ifi-ih y Batch No / /Y ^ cm. Test Solution Volume 6________ Liters/Tank No. Organisas per dose ^ _______ . Time Started / ) A/ Date Started /^/e Date Completed jo /t t m Dilution Water Carbon filtered well (Temp. .a wcautceir IL C ., D.O. lo Investigato 50 (95% Confidence Limits) hS (Full Signature! ppp pi b j3 1.7 *y ) Cone. Total Amount Added ywnvi Control 24-1lour:3 Total ' D.O. No. Dead JL PP* o J t l fr* 1 C . I t ____ 0 2-M . .LA . PS 1 '* IS is o 1 * (,.0 48-H<jurs , 72-Hours Total D.O. Total D.O. No.Dead pH -J>P N o .Dead P PP 7-1 P ._i>______ ?,9 L %k i 0 ?<* J o A l 11 /.2, t 7-7 X a 7-7 J . i )S f 0 1 \ ____ _____ ?* .1 *3v' CJ > J- 1 n S < S to to Ua A >*LJ _____ Test Temp, c IJ* ignature li Date 1 lOfnllV Comment s: . .4 r 3 A l N L .<L 96--Hour`S Total Dy No.Dead PP O 2. 1 * /* , * & Q St\ i f J.c 7 1 b'i ? . / I'O . /*** -1W fe/btfit Effects other than Mortality Cone. mg/1 Symptoms or Reactions Control xHAtPAkJUA*T* lunature it Date Comments : 24-Hr. 0 0 I JL H BESr COPYavailable Total No. Affected 48-Hr. 72-Hr. 96-Hr. 0 10 to 3 I --- fJM tL - ^ i 0 7 ..... _____ --' tout t it -0 0 2 5 5 3