Document 2bzZoz5rBpr0yYBOoVmdGXz5

FOR DU FONT USE ONLY AR226-2944 Du Pont HLR 444-89 Study Title ___ Static Acute 96-Hour LCSOo1 Fathead Minnows (Pimephales promelas] Author David G. Hutton Study Completed On December 5, 1989 Performing Laboratory Haskell E. I. du Pont de Nemours and Company, Inc. Laboratory for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50 Newark, Delaware 19714 Laboratory Project ID Haskell Laboratory Report No. 444-89 Page 1 of 9 Company Sanitized. Does not contain TSCA CBl Ou Pont HLR 444-89 GOOD LABORATORYPRACTICE STATEMENT This study was conducted according to EPA Good Laboratory Practice Regulations (40 CFR 792). Any areas of noncompiiance are documented in the study r -ords. No deviations existed that significantly affected the validity of the study. Submitter: E. I. du Pont de Nemours and Company, Inc. Sponsor: Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Study Director: ^fc^L ^)' ^^^ZJfci. David G. Hutton S-f f& fST Consultant Environmental Sciences 2 - Company Sanitized. Does not contain TSCA CBI GENERAL INFORMATION Du Font HLR 444-89 Material Tested: Medical Researchjto, Haskell No.: Purity; Synonyms: 17.931 Other Code: Sponsor: Material Submitted By: Study Initiated - Completed: Notebooks: Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Wilnnngton, Delaware Inc. lemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Jackson Laboratory Test initiated: Test completed: Study completed: 7/14/89 7/18/89 7/2&/89 There are 9 pages in this report. Distribution; - 3 - Company Sanitized. Does no\ corrtamTSCA CBl Du Pont HLR 444-89 Title of Report off.___ Static Acute 96-Hour LCSO8 Fathead Minnows (Pimephales"promeTas] Summary __ -17.931) exhibited no significant acute toxicity to fathead innows (Pimephales promelas). a warm freshwater fish, under static unaerated test conditions during a 96-hour exposure period. The 96-hour LC50 was greater than 4000 mg/L because only 5 percent mortality occurred in each of the 2700 and 400C mg/L concentrations, the highest concentrations tested. Obvious insolubility was observed at all test concentrations from 800 to 4000 mg/L. Reported by: /- ^fm&L. V^---- Deborah L. Taerchhnn-itcrian TyierY\ ^ f/.l/ff Mo7Dajr/Yr Study Director: sU Z<^l^ ,{?, H <^Z<>rv David G. Hutton Consultant Environmental Sciences ^ff7/^ Mb/Day/Yr Approved by: ___ Frank X." Manager _Ph ___ fiTi^," Ph ~ _.D. Environmental Toxicology and Information Services Division Reviewed and Approved for Issue: <6^W D " A >0. David G. Hutton Study Director H/ ^Mi/--^. / ^lULU? Mo/Day/Yr DLT/DGH/FXP/smk/AQRP33.5 - 4 - Company SanTfized. Does not contain TSCA CB1 Du Font BLR 444-89 QUALITY ASSURANCE DOCUMENTATION STUDY: X------------ Hf 17,931 StFaattihceaAdcuMteinn9o6v-Hs ou(PrimLCep50haoleis_p_ro_m_e_las) AUDITS: Items Audited Conduct Protocol, records, and final report Audit Dates 7/14/89 8/28-29/89 ^ B SHORT-TERM AUDIT REPORT NUMBER: DATE FINDINGS REPORTED TO MANAGEMENT AND STUDY DIRECTOR: 8/29/89 Reported by: X^--^. ^2^- Villiam J. Lynain Quality Assurance Auditor //A//ff? Date 5 Company Sanitized. Does not contain TSCA CBI Du Pont HLR 444-89 Introduction/Purpose o^W^fl To determine the static acute toxi'city minnows (Pimephales promelas). a warm -- fresnwater (H-17,931) to fathead Materials/Methods (Aquatic SOP-T02) ||HUf The test material, was placed into 5-liter aquaria, and diluted with laboratory well water ; 3 yield the desired nominal exposure concentrations in 4 1ite ''al volumes (11.5-cm liquid depth). Two identical vessels, both co'it.dining only laboratory well water, were designated as t-heHnO controls. Chemical stability of the test and control materials in well water was not determined. Juvenile, unsexed fathead minnows (Pimephal es promel as) from Hasken-bred stock with a 1.9 cm mean standard length (range 1.2 to 2.5 cm) and 0.079 g mean wet weight (range 0.020 to 0.194 g) were randomly placed in the test vessels, 10 fish per test vessel, two test vessels per concentration. Loading was 0.20 g fish/L. Fathead minnows are identified by labeling the culture and test vessels. The fish were maintained at Haskell Laboratory for approximately 47-53 days, with the last 21 days in a 272-liter Fiberglas tank (84-cm in diameter by about 49-cm liquid depth) before being used for this test. Holding temperature was within 5C of testing temperature throughout the holding period. The dissolved oxygen concentration during acclimatization was assumed to be at or near saturation because air was continuously bubbled through the water. No treatment of the fish for diseases was required during the holding period. Fish were not fed for approximately 48 hours prior to nor during the exposure. The test solutions were not aerated, and temperature was maintained between 21.8C and 22.2C. Photoperiod was maintained at 16 hours 1ight:8 hours dark. Mortality counts and observations were made approximately every 24 hours during the 96-hour exposure period. Dissolved oxygen and pH were measured in the H0 control and in the low, medium, and high test concentrations at the beginning of the test and at approximately 24-hour Intervals during the 96-hour exposure period. Total alkalinity, hardness (EDTA). and conductivity were measured at the beginning of the test in the H,0 control. Records All records from this test and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) or archived at the Du Pont Records Management Center (Wilmington, Delaware). ) Company Sanitized. Doss not contain TSCA CB( Du Pont HLR 444-89 Results Test results for fathead minnows exposed tofHflR(H-17,931) are presented In Table 1. The 9$-hour LC50 was >4000 mg/L, because there was only 51 mortality each In the 2700 and 4000 mg/L concentrations, the highest concentrations tested. A11 test concentrations from 800 to 4000 mg/L remained very milky throughout the test, to the point where the fish were hard to see. The chemical and physical are reported In Table 2. acceptable ranges. o f f parameters measured during the test All parameters for this study were within Discussion/Conclusion has been tested for acute toxiclty with fathead minnows (PImephaTes , a warm freshwater fish. Under the conditions of this test, was found to exhibit no significant acute toxicity. In a rangefinding test at nominal test concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L. 201 mortality was observed at 24 hours in the 1000 mg/L concentration. Footnote ^050 That concentration which is lethal to 501 of a test population during the specified time period. Company Sanitized. Does not contain TSCA CBI Du Pont HLR 444-89 TABLE 1 RESULTS OF A 96-HOUR STATIC ACUTE TOXICITY TEST MITH FATHEAD MINNOWS {P1nep^1t>^>Mme1as) EXPOSED TO) Nominal Test Concentrations (mg/L) HgOControl 800 1200 1800 2700 4000 Cumuliitlve 24 Hr. 48 A* ' B* A* Observe!j Hr. B* Morltallty 72 Hr. /i^ B* 0 0 0 0 0 0 0 0 10 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 10 0 10 0 10 0 W 96 Hr. A* B* 0 0 0 0 0 0 0 0 10 0 10 0 ""Replicate exposure chambers containing 10 fish per chamber Company Sanitized. Does not contain TSCA CB1 Du Pont HLR 444-89 TABLE 2 RESULTS OF PHYSICAL AND CHEMICAL PARAMETERS MEASUREDDURING A 96-HOUR STATIC ACUTE TOXICITY TEST WITH FATHEADMINNONSEXPOSED (H-17.931) r .^Ical/Chemlcal raidmeters Dissolved Oxygen (mg/L) 1UomfnalT< t; Concent rat itns (mg/Lr 4906 1506 606 (H^gh) (Medium) (LON) H0 (Control) 0 Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. H 8,7 8.7 8.8 8.7 81 8.0 8.1 8.1 7*3 7.5 7.7 7.7 6,3 7.1 7.4 7.6 6.1 7.0 7.3 7.5 0 Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. 7*5 7.5 7.5 7.5 7.7 7.7 7.7 7.7 7.7 7.7 7.7 7.7 . 7.4 7.4 7.4 7.4 7.5 7.5 7.5 7.5 Water Chemistry on the HA Control at 0 Hours Total Alkalinity (mg/L as CaCO,) EDTA Hardness (mg/L as CaCOil 69 68 . CornJUC;t1v1ty (Pnhcis/cm) 170 D 9- Company Sanitized. Does not contain TSCA CBt