Document 2b7kYNaL8b70bNRjKZ5bJEXb

Material Tested Copies to: E. I. du Pont de Kemours and Co-npany Haskell Laboratory for Toxicology and. Industrial Medicine HASKELL LABORATORY REPORT NO. 276-73 Haskell No. 7944 rganic Chemicals Depa AR226-2837 PRIMARY SKIN IRRITATION AMD SEMSITIZATIOM TESTS ON GUINEA PIGS Procedure; The test for primary irritation was conducted by applying, and lightly rutting in, on of a 50% and undiluted solution (wt/vol) of the test material in distilled water on the shaved in of 10 male albino guinea pigs. To test for the sensitization potential, a series of four sacral injections was given, one each week over a three-week^ period^ whichcp.^iste.dLof,0..3,.ml Qf a-1^.0^ of test material in distilled water. Following a two-week rest period, the test animals were ch sensitization "by applying, and lightly rubbing in, one drop(~ 0.05 inl) of a 5C^ and undiluted so of test .material in distilled water on the shaved Intact shoulder skin. A group of 10 previously guinea pigs received similar applications at the time of challenge to provide a direct compariso reactions on skin of similar age. Results Concentration in distilled water Primary Irritation Test 24 Hours 48 Hours Challenge Test 4 Hours '^'i Hours Number of animals sensitized-^ Reactions* on Intact Guinea Pig Sk Test Areas (lO Animals - In. Avg. Wt. = 475 S Final Avg. Wt. = 704 g) Control Are (10 New AniiPil As Received 50/o As Received 10 negative 10 negative 10 negative 10 negative 10 negative 10 negative 0/10 10 negative 10 negative 10 negative 10 negative - 2 Summary a| ^------^flproduced no irrltat-'on or sensitization when tested, on guinea pig skin at 50^. in distxTIecRaterand when undiluted. Alt' igh no serious skin reactions occurred, good ind hygiene practices should be used -when handling an., chemical. Reaction Code: +, ++, .+++ = mild, moderate, strong erythema; ++++ == erythema plus edema; +++++ ** Sensitization is defined as a iwo-step score increase (-<-+) at challenge over the response expect same amount applied initially or on the concurrent controls. Report by: ^^.- AM 0. Louis Dash OLD:righ May 2-:', 1JJ-:, Approved by: / \ C^A g-^gtf ^"7 J Charles F. Reinh Assistant Direc