Document 2aw95Q83bQQxgXLyKeQ6KwmN

From: To: Subject: Date: Attachments; Brower, Jeremy Dovle-Eisele. Melanie: Irshad. Hammad: McDonald. Jacob RE: EUGT abstract Wednesday, October 7, 2015 11:36:43 PM SOT 2016 diesel NHP abstract submission.pdf Thanks for your feedback guys. Abstract has been submitted. We can probably change the authorship list later if necessary. From: Doyle-Eisele, Melanie Sent: Wednesday, October 07, 2015 4:40 PM To: Irshad, Hammad; Brower, Jeremy; McDonald, Jacob Subject: RE: EUGT abstract You may want to refine the final statem ent and remove the word "great" in front of inflammation. You can soften the overall statement since the data is still coming in stating that inflammation was observed after both exposures. From: Irshad, Hammad Sent: Wednesday, October 07, 2015 4:34 PM To: Brower, Jeremy; McDonald, Jacob; Doyle-Eisele, Melanie Subject: RE: EUGT abstract Looks good to me. You may want to mention that target particulate mass concentration for OTD exposures was 300 microgram/m3. Also added particle size distribution to exposure characterization (below). From: Brower, Jeremy Sent: Wednesday, October 07, 2015 4:28 PM To: McDonald, Jacob; Doyle-Eisele, Melanie; Irshad, Hammad Subject: EUGT abstract Thoughts on this? I tried to leave the results very vague in case Stephanie's results don't match the rest. Exposures to Old or N ew T echn olog y Diesel Em issions to Evaluate Biological Response in Non- Human Primates The adverse health effects of environmental exposure to standard diesel vehicle emissions are a major concern among urban populations. While it's been advertised that new technology diesel emissions include a much smaller particulate fraction, confirmation that this results in few er adverse health effects has not been established. This study evaluated the biological responses of female cynomolgus macaques follow ing acute exposure to filtered air (FA), new technology diesel (NTD), or old technology diesel (OTD) emissions. Exposures were conducted in whole body exposure chambers and lasted 4 hours in duration. The exposure atmospheres were characterized for aerosol mass concentration, particle size distribution, carbon monoxide, carbon dioxide, nitrous oxides, and hydrocarbons. NTD contained particulate matter similar to FA control exposures, while OTD had approximately 40 times the particulate content. Hydrocarbon content was similar among exposures while all other gases measured were substantially higher for OTD exposure than for NTD exposures. At baseline and at 4 hours post the end of each exposure, blood was collected for hematology and serum collection, lung lavage was performed, and bronchial brushings and biopsy were conducted at two sites within the bronchial tree for various endpoint analyses. At 1 hour post exposure an additional blood sample was collected for measurement of hematology parameters. Lavage and serum samples were analyzed for measurement of various cytokines. Sample analysis continues but, contrary to the hypothesis, NTD appears to have Induced great inflammation by measurement of several key parameters. Jeremy Brower (o) 505-348-9662 (c) 505-259-5386 Print this Page for Your Records Close Window Control/Tracking Number: 2016-A-3811-SOT Activity: Abstract Current Date/Time: 10/7/2015 10:34:33 PM Exposures to Old or New Technology Diesel Emissions to Evaluate Biological Response in Non-Human Primates Author Block: Jeremy Brower, Hammad Irshad, Melanie Doyle-Eisele, Jacob McDonald, Yohannes Tesfaigzi Lovelace Respiratory Research Institute, Albuquerque, NM Abstract: The adverse health effects of environmental exposure to standard diesel vehicle emissions are a major concern among urban populations. While it's been advertised that new technology diesel emissions include a much smaller particulate fraction, confirmation that this results in fewer adverse health effects has not been established. This study evaluated the biological responses of female cynomolgus macaques following acute exposure to filtered air (FA), new technology diesel (NTD), or old technology diesel (OTD) emissions. Exposures were conducted in whole body exposure chambers and lasted 4 hours in duration. Flows and dilutions in the exposure system were targeted for 300 pg/m3 particulate in the OTD atmosphere. The same flow and dilution settings were then applied to the other atmospheres. The exposure atmospheres were characterized for aerosol mass concentration, particle size distribution, carbon monoxide, carbon dioxide, nitrous oxides, and hydrocarbons. NTD contained particulate matter similar to FA control exposures, while OTD had approximately 40 times the particulate content. Hydrocarbon content was similar among exposures while all other gases measured were substantially higher for OTD exposure than for NTD exposures. At baseline and at 4 hours post the end of each exposure, blood was collected for hematology and serum collection, lung lavage was performed, and bronchial brushings and biopsy were conducted at two sites within the bronchial tree for various endpoint analyses. At 1 hour post exposure an additional blood sample was collected for measurement of hematology parameters. Lavage and serum samples were analyzed for measurement of various cytokines. Sample analysis continues but in the endpoints observed so far, inflammation has occurred following both the NTD and OTD exposures. Presentation Preference (Complete): Poster Only Category (Complete): Respiratory Toxicology ; Clinical and Translational Toxicology Perceived or Real Conflict of Interest Disclosure (Complete): The research described in this presentation was supported by an entity that manufactures and/or distributes a material that is the subject of this presentation. : True The authors declare there exist no real or perceived conflict of interest: True Sponsor (Complete): Abstract Submission Fee (Complete): Your credit card order has been processed on Wednesday 7 October 2015 at 10:32 PM. Keyword (Complete): *Keyword 1: exposure, environmental Keyword 2: inflammation Keyword 3: inhalation toxicology Attached Files: No Files Attached Status: Complete Society of Toxicoloqy 55th Annual Meeting March 13-17, 2016 Ernest N. Morial Convention Center, New Orleans, LA Society of Toxicology 1821 Michael Faraday Drive, Suite 300 Reston, VA 20190 703-438-3115 Office sothq@toxicoloqv.org http://www.toxicoloav.org/ Leave OASIS Feedback Powered by OASIS, The Online Abstract Submission and Invitation System 1996 - 2015 Coe-Truman Technologies, Inc. All rights reserved.