Document 2RywbD2Qqv8xDg1maaYmbqVpL

AR226-2848 Company Sanitized. Does not contain T S C A CBI E. I. du Pont: de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 66-76 Material Tested Haskell No. 9542-3 Other Codes Submitted bv ________ Jackson Lab., Organic Chemicals Dept. uttKurau oiseis lTlEA'ilON Ten healthy male beagle dogs were exposed to the test mixture via face " J 1! * ha^inf their electrocardiogram recorded on Lead I; the two active e l e c S S f T e ^ l S i ^ K ^ i f h t and ne* hrine iO 008 m e / S ini 1,6 ln^lf^rent .electrode midback. The test animals received a control injection of epi- , ("008 mB/k8) intravenously five minutes prior to exposure and a challenge inject-on of the same dosaee after P ssss susl-s'sjs * crux's' " te s- E* p" ute containing ^ ' a .liquid* wa8 vaPori*ad by bubbling four liters of air per minute through an "NO, Scrubber" material remaining in the I* CmP""d* *ha bber was housed in a warm water bath (38C). After each exposure the acenai remaining in the scrubber was discarded and replaced with a fresh sample. taken everv^inutrdnrra1'!"0111'"8 th ?8 WftS monitred by two sampling methods. (1) A one-half milliliter air sample was the mixture 0 33 t n ^ ^ fit P ^ Wlth " 888 tight 8yringe from the line leading to the dog mask. (2) A portion of of absolute ethanol Both the a?* ml^U^e "as drawn from a Point near the dog mask through two impingers containing 20 ml ioni tion deSctoi'and J W.. f i"?1"8" 8araPlea wera analyzed on a gas chromatograph equipped with a flame was measured anaivtinaliv c! Stee1' 10% FFAP n < * * W column. Only the main component ~ y y. Standards had been made previously by using a relatively pure (93.5%) sample of( f r S S u r e r b y ^ m p i n g e r ^ i t w a r i o r 5 tt ^ ? * ! P i ; 7 9QmeanU ced by air 8amP leS waS 110*3 11`1 <>47 >8 PP"0. relatively low in relation t m ", ~ 3,8 "'871^(5729 + 855 ppm). The concentrations of the other components were (not ^ r L i r c o m p o n e n t l decreasad ` P " " ** ^ P ^ a l l y the "high boilers." The concentration of the "low boilers" N02 scrubber changed from clear p u r p le ^ o clear"e l i L f " eXPSUre* Durlng the exPOSure the clr f khe mixture in the 'Company Sanitized. Does not contain TSCA CB1 Results (Continued) 2- None of the ten dogs tested had any serious arrhythmias as judged by the criteria of t-h* ,, .. tesM^ultiple consecutive ventricular beats or ventricular fibrillation) / During the firft min?* f sensitlzatlon arf-l. struggled-perhaps due to the presence of an irrit^ing c o S t ^ u e n i eXPSUre t0 No attempt was made to expose dogs to a higher test coneentraHnn animals were for all practical purposes exposed to a saturated vapor. * . _ .. - on e esign of the test the Summary: According to the conditions of this study, concentrations of 105-110 mg/L o ^ H t h e ^ ^ H H B H d i d not produce cardiac sensitization in any of the animals t S H ? !the main component of Date Issued: January 30, 1976 Report No. 66-76 Report by: Approved by: Linda S. Mullin Physiologist Neil D. Krivanek Chief, Physiology Section