Document 2RnqRk9e8zLB7rE0Q4xQKZeNb

A IW 6 - 5 I5 . ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, FC116, FC-126, FC-169, orFC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1 ) Remarks: 3M production lot number 390. The test sample is FC-126, a white powdery solid. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 78 - 93% test substance and 7 - 22% C5, C6, and C7perfluoro analogue compounds. METHOD: Method: Not stated. Type: Static acute GLP: No Year completed: 1987 Species: Daphnia magna Source: 3M Environmental Lab in-house cultures Analytical monitoring: Temperature, pH and DO Exposure period: 48-hours Statistical methods: Probit analysis Test organism age: <24 hours old neonates Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry (initial): Temperature: 21 C Dissolved Oxygen: 9.4 ppm pH: 7.9 Stock and test solution preparation: Test solutions were created by direct weights addition. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 250 mL Pyrex glass beakers containing 200 test solution (6 cm depth). Number of replicates: two Number of daphnids per replicate: 10 Number of concentrations: five plus a blank control Water chemistry during the study: Dissolved oxygen (final): 003865 9.0 mg/L (control exposure) 8.8m g/L (1000 mg/L exposure) pH range (final): 8.0 (control exposure) 8.1 (1000 mg/L exposure) Test temperature (24 - 48 hours): 21 C RESULTS Nominal concentrations: Bk control, 100, 180, 320, 560, and 1000 mg/L Element value: 48-hour EC50 = 221 (1 8 6 -2 6 1 ) mg/L Element value based on nominal concentrations CONCLUSIONS The test sample 48-hour ECso for Daphnia magna was determined to be 221 mg/L with a 95% Confidence Interval of 186 to 261 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA Q U A L I T Y __________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and it lacks analytical confirmation of test substance concentrations. REFERENCES . This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request Number E1282-1, completed on April 30, 1987. OTHER Last changed: 5/24/00 C038S6 ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET L R ^ /g /fc -/ TEST TYPEM f t u ^ TEST ORGANISMT T ^ U . ^ CHEMICAL TESTED In___________ ,, L O T ^ m SOURCE L g g P A r ^ L j ------ DATE REC'lL .Ry/ y i^ AGE OF TEST ORGANISMS: < 24 h rs . o ld in s ta r s (neonates) NO. ORGANISMS PER TREATMENT WATER TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200ml DEPTH: 6cm If) TIME STARTEn >S! DATE STARTED __ DATE COMP ETFn H -- 6 Q~P^j ^DILUTION WATER C a r b o n - f ilt e r e d w e ll w ate r (Temp. 2 \ C, D.O. ppm. pH *f. ) ANALYi TEST I n i t i a l VESSEL Oonc. NO. m g/1 T o ta l Amount Added en ( 95% Confidence L im its lZ i^ 1lg |j ~ < ^ k V c./L > 'W r 4 ^ ir C s , ' e d i t e d Z Z \ ( 24 Hours 48 Hours i vKqliZ> ^ a1' T o ta l No. pH D.O. T o ta l No. pH D.O. Dead ppm Dead ppm TIT Contro! H 1o n ZD \ - i IfS O Klo ^ 2 0 uA- H - > 5(^0 i~ rf in n o IZ ) zro ,0 n Zj /n W Wft/? 90 ftfl 9,0 JD fl,/ J n ft,/ fa? COMMENTS T e s t Temo. C I n it ia ls 6 D ate *1 ,9 . i^>4<yi^r iC U rk 3 adr jW^erihoflKrtHfltis^. 2/ afaggnK T fa V -.^ j-K y o r u r e u t U t ^ r u -WrsterL ) i n m ^ k oTNlud a ^ U i A<>W~~ 003867 ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET ^ -E J 2 B 2 jz T T TYPEI I & r t R - A 'i f r F C ^ r m n C ^ CHEMICAL TESTED pQ_- \Zln______________ LOT TEST ORGANISM A _ SOURCE -- DATE RFr*n *KJ l 4 K ip _____ AGE OF TEST ORGANISMS: < 24 h r s . o ld in s ta r s (neonateV) NO. ORGANISMS PER TREATMFNT WATER TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200ml DEPTH: 6cm \Q TIME STARTFO J 5 l OATF STARTED ,-DATE QOMPl FTFO M -- DILUTION WATER C a r b o n -filte r e d w e ll w ate r (Temp. 7 I C. D. 0 . _ 3 x h t -- PPm pH V . Q ) ^--y / TEST In itia l VESSEL Cane. NO. rag/1 ECcQ (95% Confidence L im its ! t l Y i ( ccnAlpltf-flcl 2~Z~l (.100? " v ^ r O k *W U Total 24 Hours 48 Hours Amount Total No. pH D.O. Total No. pH D.O. Added Dead Piin Dead nMarn T Contro! o 2 -1 l O zn _ 7 *2 - \f<n &{r. 0 7 - ^ KZO (>4 Z) 7 A 5(O IIZj lor^n ?nr> 4 / n .. O f i t Q 9 ,0 o fitQ9 ,0 y fhn fyO 4 \Q fO / o ? ,| 9,0 / / ) ff,| M COMMENTS : Test Tens*. C In itia ls & Date *= ZJ, ZJ iMAfV'i&S n f Ukjl/tW - ^ eyUcdfllp ii^ ir QrV- a*d a-eid - r>vVlS4^fvt5,_____________________________________________________ 003888