Document 2RQxpo06qvjQzjkYg55QQOy8p

AR226-3049 Study Title Approximate Lethal Dose (ALD) of H-21953 in Rats I ohomtnry Project ID Haskell Laboratory Report No. 795-96 Author Tracy A. Filliben rnmpleted On Septem ber3,1996 pprf^rrninp t iihoratorv E I du Pont de Nemours and Company Haskell Laboratory for Toxicology raid Industrial Mediane Elkton Road, P. O. Box 50 NouMirlc Delaware 19714 Sanitized. Does not contain TSCA CBl Company Approximate lethal Dose (ALP) ol H-21953 in Rais TQENERA^tiNFQRMATIQK giiKciance Tested: S ynonvms/Codgs: nuPont HLR 795-96 Physical Form : Composition: Opaque tan to white liquid Contaminants: Purity: Submitterji N otebook^j.: CAS_Ri^ D lN ^ : Sponsor: Study. Initiated - Completed: DuPont Specialty Chemicals F.. 1. du Pont de Nemours and Company Wilmington, Delaware 6 /2 1 /9 6 -9 /3 /9 6 Company San In Life Phase Initiated - Completed: 6/22/96 - 7/8/96 All raw data and the final report will be stored In the arahivea of Hashed l ^ o naory t o Toxicdoev and Industrial Medicine. E. I. do Pom de Nemours and Company. Newark. D elaw ar^ir al Iran Mounlain (fonnerly DoPom Records Management Center. Wilmington, Delaware). Company Sanitized. Does not contain TSCA CBS Approximate Lethal Dose (ALP) o f H-21953 in Rats SUMMARY DuPont HLR 795-96 H-21953 was administered as a single oral dose by intragastric intubation to male rats. No deaths occurred during the study. Clinical signs of toxicity were observed in non-lethally dosed animals. Under the conditions of this test, the ALD was greater than 11,000 mg/kg of body weight. This substance is considered to be very low in toxicity (ALD greater than 5000 mg/kg) when administered as a single oral dose to male rats. - 4 - Company Sanitized. Does no! contain TSCA CB! Approximate Lethal Dose (ALD) ol H-21953 in Ruts Sir.NATURE PAGE DuPont HLR 795-96 Report by: ^ uJ j J (j Tracy A. Filliben Toxicology Associate Work by: ( ~ (j Tracy A. Filliben Toxicology Associate Reviewed and Approved for Issue: Tracy A. Filliben Study Director C i,3 A L TF/alw -5- Company Sanitized. Does not contain TSCA CBi INTRODUCTION DuPoni HLR 795-96 The purpose of this res, administered w h t c S s r f d e a l h either on the day of dosing or within 14 days post exposure. M a t f p i a i S AND METHODS A. Animal Husbandry _- - /-ti r rt0,cniB R rats aDDroximately 7 weeks old, were received from Charles fS w iC S Rats were housed singly in suspended lve. -1 wire mesh cages Each rat was assigned a unique identification number J E S r i 2 R i S d to the cage. The rats w em tail-m artced ^ n g a :L e r Z l u b l e marker, with Ute 1 3 digits of the animal number. Forma. Cemfied Rodent Chow #5002 and water were available ad libitum. 5SgS5gfHaskell Laboratory has an animal health monitoring program This program is Data have interfered with the results of this study. Ra,s were quamn,ined: ^ rhour^l.g h t2m -t ha ^r 'rrdracryk cc yt e. cE^n L n m en tal conditioonfs5o0f%thero, ooma s wEsecreurtsa.rognested iuuside'ihie ranges were of small magnitude and/or brief duration and did not adverselv aftect the validity of the study. Protocol The test substance was administered neat to I rat pertlose rate f I,aba,ion. The density - The D o* administered ranged from 2300 to , 1.000 -h - Company Sanitized. Does not contain TSCA CBf Approximate Lethal Dose tAl.Dtol H-21^53 in Rats DuPont H1.R 7*)5-% mg/kg of body weigh in increments of approximately 50%. Additionally. I .at was dcised at 670 mg/kg. The dosing day was test day I; postexposure day 14 was test day IS Following administration of the test substance, rats were observed for clinical Observations for mortality and signs of illness, injury,o r Z* daily throughout the study. Pathological exam.nat.ons of test animals were not performed. RESULTS A. Hnsape and Mortality Data The dosage regimen and the mortality resulting over the 15-day test period are detailed below. No deaths occurred during this study. Dosage (mg/kg) 670 2300 3400 5000 7500 11.000 Dose Volume (mL) 0.18 0.61 0.83 1.1 1.7 2.5 Density fmg/mL) 1030 1030 1030 1030 1030 1030 Initial Body Weieht (e) 280 275 250 236 234 238 Mortalil No No No No No No Company Sanitized. Does not contain TSCA CBI Approximate l.ulhal Dost-* (Al.i)) I H-21953 in Rais DuPont HLR 795-96 B. Clinical Signs Nonlethal Dosgs No clinical signs of toxicity or weight losses were observed in the rats treated at 670 or 2100 mg/kg. The rats treated at 3400 and 5000 mg/kg exhibited lung no.se I day after dosing: the 3400 mg/kg rat also exhibited lung noise - days after dosing. i Weight losses of approximately 2 - 1 2 % of initial body weight were observed in rats treated from 3400 - 11.000 mg/kg I day after dosing. CONCLUSION Under .he condUions of .his shrdy. .he ALD for H-21953 was grearerUranj LOOP mgfkg of body weigh.. This substance is considered lo be very low in oxrcrty <ALD greater than 5000 mg/kg) when administered as a single oral dose to male rats. Company Sanitized. Does not contain TSCA CBl