Document 2RJ30vVygxL6y31daEoyo1NNg
AR 226 _ 0850
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
IESTSUBST ~~AN0C 000E 0
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m7a8y7aolrsoFCb-e 787X. (1-Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 101. The test sample is FCd7i8e7t.hyCluernreegnltycionlfomromnaotibountyilndeitchaetre,s 7it9i.s7a4m%iwxattuerre,of5%0.e7t3h%ylPeFneOSgl,yc1ol0,%1.47% spurlofpataen,e0S.u7l5t%onxeafnotahmaenrg,u0m.,703.%7s5o%dimuamgnocetsyil usmulfsautlef,at0e,.703.%10s%odNi-u(m3-decyl chioroallyllhexaminium chloride, and 0.05% benzotriazole.
cThhaerafcotlleorwiiznegd scounmcmenatrryataipopnlsioesf tiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD:
0000000
Method: OECD Guideline 202.
Type: Acute static
GLP: No
Year completed: 1990
Species: Supplier:
Daphnia magna In-house cultures
maintained
at
EnviroSystems
Division,
Resource
Analysts, Inc., Hampton, NH.
Analytical monitoring: pH, temperature, conductivity, and dissolved
oxygen.
Exposure period: 48-hours
Test organism age: <24-hours old
Statistical method: The EC values were calculated using a computer
program by Stephan, 1983.
-
Test conditions:
Dilution water: Filtered natural well water
Dilution water chemistry:
Hardness: 180 mg/L as CaCOs
Conductivity: 1300 pmhos/om
Lighting: CooplH-:white fluoresce8n.t1 lights at 18 E/seci?. A daily photoperiod.
opfer1i6od.hours light and 8 hours dark was maintained throughout the testing
Stock and test solution preparation: A 1000 mg/L stack solution was
prepared Exposure
bcyondciesnstorlavitnigon1s
g of test substance in 1000 mL dilution water. were prepared by additionofstock solution to
the
test vessels.
002624
Exposure vessels: and awaterdepth of
2a5pp0romxLimgaltaeslsyb6eackme.rs
containing
200
mL
of
test
solution
Number of replicates: 2
Number of daphnids per replicate: 10.
Number of concentrations: five plus a blank control
Water chemistry during the study: PH ran8g.e0(-08-.418 h(coounrtsr)ol: exposure) 8.0-8.3 (1000 mg/L exposure) Temper1a9t.u8r~e20r.a9ngeC((0c-o4n8trhooluerxsp)o:sure) Conduc2t0i.v0it2y0r.a9nge:C ((01-04080hmogu/rLs)exposure) 1300 - 1300 pmhos/cm (control exposure) 1200 - 1300 pmhos/cm (1000 mg/L exposure) Dissol8v.e5d-o9x.y0gmegnlLra(ncgonetr(o0l-4e8xphoosuurrse)): 8.0-9.0 mg/L (1000 mg/L exposure)
RESOTS
0000
Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mglL.
Element values: 48-hour ECso = >1000 mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on the mixture as described in the Test tShuebsftluaonrcoechReemmicaarlkpsrfoipeoldr.tiTonhealvoanleu.es reported apply to that mixture and not
co0 nclusio0 ns 0000
`The test mortality
substance 48-hour or sublethal effects
ECso was determined were observed.
to
be
>1000
mg/L.
No
-
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
p0 ata0 Quar0 y 000000000
tReesltiianbgi.litHyo:weKvieirm,istchhersanakmipnlge=pu2r.iTtyhweasstundoyt mperoeptesrltyhechcarritaecrtiearfiozrqeudaalnitdythe -- study lacks analytical confirmationofthe amountoffluorochemical proportion
in the solution.
002625
RE0 FERENCES0000
This Inc.,
study was Hampton,
NcoHndatucttheedrebqyuEesntviorfotShyes3teMmsCoDmivpiasinoyn,,
Resource Analysts, St. Paul, MN, Lab.
Request number H1609, 1990.
omWER
0000
Last changed: 6/28/00.
002626
Study Title
Static Acute Toxicity of to the Daphnid, Daphnia
FC-787 magna
Authors Timothy J. Ward Robert L. Boeri
Study Completed September 4, 1990
Performing Laboratory
ResoEuarvcieroASnyalsytsetnss, DiIvnicsoiropnorated
?.0. Box 778
-
HamptoOnn,e
Lafayette Road New Hampshire
03842
EnviroSysteas Study Number 9070-3 Page 1 of 16
RescurcsAnclyt inc. SubsdiayofMILPORE 002627
on
I. ooh LABORATORY PRACTICE sATONERT
Bi
|
PTrraiccteiTchei.osf Reagnsuytluadtyciiornwcsau.ssstacNneocinetdhsuecrtetdatthescvoooSutrludddiyngDaifrfteeocctto0rtDheooriGnottohedegristpLyoanbsooforrattohariryse
,
VSIetpruedecyi.aernasndTohnweat'caon.dt,esatdadneddsubtso`Tthatenecsettesvtweasssevlassstoircneoddnidsuc`crstiteadtnreotowmeiltyhtea3nadpreatrteashtterrev,etshsteaelsn4st
A
TTeaplliiaciattyeso,f theWostudfyv.istions existed hat :significantly atfected the
|
TCoo0nvnatLrhor1fnSda7rSltudGyorector94-90
Taiaotfhyorof ha
#/slo
Sefiae 7. Fugen
5.96
Aabatic Toxicordatst
RatilToexienstotptset 5 neSon
BPaavgter'ossyoafte1n6s study Nusber 070-3
[OR -- 002628
i
3 '
E g
"
+
oo
IL. QUALITY ASSURANCE STATENENT
Suaitted by: ERPeaosvroiurrohcseeyxstATeahnsslyaDtisv,isiIonncorporated OBnaeapLtoanf,ayeNtexteRRaosapdshire 03042 -
Certitications
FDineaapt'osrtvpirtheesnetnmitattelederdiailn"setthh`oiadsesnrteaipfnoidretdNatvee`irrneisdlteehr.ei"vseedTchteibyontmeseotfthovtdahsse
TTe0b7t0o3r,asdwitinh Regtstiation.
atAchgceuoartdinacontceed.ToxvsixicctoehtpotniEroanvsiicaobsaoinrsdatteosrtsyhe PrSPtoeatonofdcueoccltd
OpBpoeeerrrtaiot,risnegd.undPbyroTcieJodetuahrnyees VMaKarandg.uaazlo., ThPeeTtheoerr.igiotnousrt]iieliftasyv,deastRtaobevarantsd
FTaien:aforre4p%ortdesWtAL.10 byeearsa.rchired st Resource Anaiyats,
VBMye1roqudaa"GlthieetcykterdAasnssufcrorarnicbeeadccauwrafairctoyon,rs.atnhdeQualrlalyitddyaattssassvvteroraantcheeverraieufpdioiertdts
vere pertoreed on
rDaote. stRuepdoyrtDeidrecttoor MRaenpaogretmeedntto
s7ar7r8si/a%s0 s83a/71247s7230
180/255//3900
10/25/90 10/25/30
10/35/30
)
QBuoirteintyNeAvsbs2ouri5an..neceRoettailceer ag L0R5-50
BTaagveiro3Syaste3n6s Study Nusber 9070-3
Co
Aussies Araiph Sods SALT
I
002629
IIL. TABLE OF CONTENTS
section:
Pace
5
I. Good Laboratory Practice Statenent _
2
I. Quality Assurance Statement
3
HI Table of Contents
`
-
Iv. Index of Tables and Figures
v. summary
5
.
6
VI. Introduction
'
VII. Methods and Haterisls
7
IIL Results
10
m. Reterances
1
Appendix A. Vater Quality Data from Toxicity Tost
1
EPangveico4syofste1n6s study Nusber 9070-3
oo
Co
Breaks SAARI
| 002630
IV. INDEX OF TABLES AND FIGURES
PAGE
Table'l. Cshaemmpilcealofchnaartaucrtaelriwezlaltiownateofr @usredepraessednitlauttiivoen
s
#
water for toxicity test
Table 2. Survival data from toxdeity test
n
.
Table 3. Mterdoinantoexfifceicttyivteestconcentrations (ECS0s)
1
Table A.1. Cotoxenystdguecnticvointcye,ntrpaA,tiotnempmeeraastuurreed,duarnidngdistsooxlivceidty 1
EPaagveiro5syosfte1n6s Study Nusber 9070-3
Srossseorove. SstAysueiron
002631
v. sumary
describeTdhe 48 hours
inacuthties during
tfionxailcirteyporotf. July 23 to
FTCh-e787testtowasthceonddapuhcntiedd, foDrap3hniCaommpaagnnay, foirs 25, 1990, at the EnviroSystems Division of
RJtheeeasnonuperrcoetoMcaogAlanzauld,yesvtePsle,otpeerdInKcof.voarlsEiknniv,irHoaRSmoypbtseotrnet,msBNoeeSrwtiu,RdaymapNnsudhsibTreierm.ot$h0yI7t03aw.arsd caocncdourcdtiendg btyo
concentrTahteionstestof temperature of 20
wtaesst spuebrsftoarnnecde anudndera + 1C. The dilution
dsitlauttiiconcownadtietrionscontwriotlh water vas filtersd matural
aftivea well
wleeamvtpeellro.yedcoNloltmeoicntmaeldainctofanriconenntrwdaeitlsilsosonlsveatdofoHaxFmyCp-gt7eo8nn7,cvoNenreceew:ntHra0amtpnisgoh/inLrse(.acboonAvteerorlaa)tn,ioan1c5c0ewapsmtga/bnLlo,et
u2s5e0d f59o/rb,all40c0anlgc/uLl,ati6o0n0s.ng/L, and 1,000 ng/L. Nominal concentrations were
oafnd exveproDesiuhrneliedsssthethuasnecdon2trihonolurdtsahepohlnidtdessatthatwdheereanstpaarrvotedruacogefdethfweertomtewseatin.ghitnA-fh(tobeulrsoet4t8ecduhlodtruuyrr)se
oifn the
gt0oe.so0td002scuobgns,dtiatnricoeensurlaettsiunltgtheedinbeinagianlnoia48ndgihnogoufrratEthCeeS0softuodf0y..0> 1 1,Eg0/xL0p.0osnugrA/elLlofdFCa-pd7ba8mp7ih.dnsidswerteo
EPaavgiero6syosfte1s6s Study Nusber 9070-3
oe
a i ve
.
002632
VI. Demovuctron otbhjeectdaipvThehniisdo,f sta utdfheyreshsvwetasutdeyrspovinanssvoerrtetoedbdrebatytserI.aMinCeTohmeptahnerye,pAocruSttt.ecoBrnaotawalii,cnist'MyisnenofecstoiFtoEan1.s87tThehbtee dotfehescar"tithdeeer{qmtuhveaelstiitmgyeatthdioaodtnsa.caonldTlheemcattreeedrpioardtluscianlsgsaopltcohyoeendttaeisitnn.stahne satpupdeyn,dixandthattheprreessuelnttss
VIL METHODS AND MATERIALS
fl
TEST suBsTancE:
EaviroSyCs1te8a)s bottle that
on(avMiaryoSy1s5t,ens1990.SamplIte was labelled with the
vaNsusbceorntai2n0e6d95)in v4as1 gadlelliovnerpeldastitco following information: ~Let 101
R
BN
dsStua.rkfacPtauaatln',ta/rbWouiotmmynlestoetmcspa.errblaatlu,rPer.io5r-10-At9o0"ru.seesertvhFeeC-1t8se7asmtpvlamesatesruiiplapllliewdabsebystaor3rcheidCvoesidpnantyah.re
S
Eavirosystens for 4 ainiaus of 10 years.
DILUTION WATER:
|
tBousmtpisohgirWea.tvearsWautcseoerdllveacfstoerdstoarfcercdolinmiantwie5ol0nl0s-gofalalttoenstEnpvooilrfygoeastnyhisystlmeesnnseaadtafnnfkosrRawamlhpletroetno,ixticvHiateryw
:
aareerapterde.sent"eRedsuilntsTabolfech1.eaical analysis of a representative sasple of water
TEST oRGNISH:
be
E.
ussoeudrceinJu"avtnehdneilwteaersetdavpeihrdneeindtpsirfoideuedcmepudlsoifenrdognanaanstpapt-rehsootpursisaotregcaunltitasuxmrosenownaiencrdevkeefyrr.somleaDsaspshinntighdlasen
Bg
n2t4uhientheociuornnsecdluosliidonn10ao0tf8tdhietlhuesttiaortntoxiwofactiettrhyeunttdeesesttr..staPCtorinictorroclotnoddiatptiheosantiisdnsgi,nve4dreap1hiwateieirdgshegdlvaesraset
Jebareregs'i.nnifnregs"Duorofifngthaeapcpcalrtieemnstat.tiosnicDkudnraeipsnhsga,idtsheivnejarucrecileinsno,tatitoarnnedstpeedrsibofndoorr3ad4liishteoiauesrses
aantd thtehye prior to
x
tthreouttescthow,ini`tainadtfioorn or twice daily before
tthhee the
tefmrpeesrhawtautreer test.
waaslga20.S0eCl.amsDsatprhunsidcsapwreirceorfneudtuyseaosntc,e
EPangveico7syofste1r6s Study Nusber 5070-3
hl ia
002633
:
Tae 1: Cnhaetmuircaallwelclharaatcetreriuzsaedtioans dioflutiaonrveaptreersenfotratitvoexicistaymplteestof
P--a_r--ameter _--
"
eUansiutzeonfent 5 units
ReLpoirntting -
WVeaxlsuuered 51
:
Conductivity
ushos/ca
-
1300
Hardness
nolL 1s Caco -
10
i
opregsatnioccihdleosrine
wr
05
oe
:
Opregsatniocpihdoessphorus uel
5.0
oe
k
Pbioplhyecahyllosrinated wrt
0.5
woe
"--Not_det--ect--ed sbove the reporting Tinit.
0
EPangvei'rsosyosfte1a6s, Study Nusber 5070-3
td n
002634
|I
1I EB k 3 FB f 4] h :
_
frp--
u836lyaeair2Ab0u.rtseec3r0es3tnh"het3rns4g./119wt0%ae;0sstwSavNwloientsihun3rav0lti0hvecaslog/nttcea,settnta1rns,adu0t0bi0s1ot,na0nsn0g0c/Leo,fnvga/t3sLe0.s%tcosnsMudurtubvecsirtvteaadln4c5deauhtroviue5nrr0gse0
20/0, 0nd 1008 survival at a1) other tested concentrations.
T1o9n%t,eirTsheeeToernddteifeinWiitttoih.ve orl hme by the
FEtaEovTxi8ir)coistyyantdetsesstThvTeaesstDpaePprhrafoiotdro,nceodl study Director on July 30,
Dd9au0pr7hi0nn-ig3a 1350.
d(umslatygantai2)c)., toAwchui35tc,eh It is based on
Drocedutas of the OBCD (1980).
fTivSe cocoTohnceeanltttr.easttiosntsvo.acsk cofo"snodluLucettsietodn Substance "With "aiietion ater
satu`vbasastaptnarcreegpeatreadntdebayp4adrciaotlauubrtieinoionnfgva210t.e0cg1cooCnftrvtoielts,ht asd adjusting the final concentration to
T10e10n0"t56namraa,teraiJnaidlth1o,uv0te0r0e:ntgh/eL0.useBaolf a(scoolnvternotl.l, N1o5m0innagl/L,con3c5e0ntagr/akteion4s00ofultlh.e
cTeemptleircsa.rtevretnhta5yt rptentastely
dc6a`oepnihccthaaa.ii)dnseTdresvteT20rae0ess.attc:a31nvdeToshssoelfeylste`atsaetdrsetveraeqsusaolprlleauyrtnfiddoooirnasnlteyrd(ivbiaauncttreera3d5n0gedadmaalopneghlientvswansso
oTPDhniEoemitceootstpstroiftrhhofeadutrvTioe"orpsgkrtoivsoiu`ndtt.ehodeaosf4ta8iechaaMoclahulhrytveeassssiteinltt)e(a:3nisnrieaAtdnyd1o6noifhtomhuura1b1ceiorogslvh-htvtsba-aibinltwdest.vfalhsuooAuureresrseacddteainrottkno
Eeteptrablefetqouvierlesd:. Dtiophmaaiidnstaveirne dniostsofledveddurionrgygetnhecotnecste.ntrations shove
SSiaHomEaoIhtiSiTMtho)enentiJo`oomnnrsabo(t"ieohpfaebririoeiffstluyeb'rsl,uerttvohiaevlxicsoivgtaiafbfieolcrwitgitsatynh,iisn(mlsdoi1ss5scwoodlfsoercsaoqttnuhideioslni,borc`aicofurutare,rrcehnaecgnreegrneattloiifecn
SSiibaasvoiTliovete}dhouovrexsry:egendet`(ba1ar8a1aditnMeesodtdveilosrugra5ln1liysnmseatnedvre;rrsecironersmdtorevudemedinntvihteimnaulslbfyeirrastnFdRLo-ba1ec8et)re,vredp2.6i
rPoheeeictim?oemnp. TeCorind
mSFoeeduterl,eomontpsetHre,r: 13instathsreturesmreo;smteteirnnusmtbroeuersa.ebnetrPAaLu-23a2)b1.ie)r dully in each test chasber that contained
aPnRdvLe-rf4ee)s,pmaecrwoasntuduurrceetdi(vAiaStnTyd 1ive animals.
smatzstiea weTaoDs: standartJesusittastistoficalthe tetcohxniicqiuteys
t(eSsttephicno,uld198n3o)t bebecausfeategrreparteetredthebny -
SE Iurvivel sceurred at all tested concentrations.
EPnavgie'csosyofste1n6s Study Nasber 3070-3
come AAG
|
002635
VIII. RESULTS
remainedAllfreteestof veissnesloslubcloentamiantienrgiaFlC-7t8h7rouagphpoeuatredthecleteasrt.iniBtiioallolgyicaanld
and water presented
quality in Table
data 2 and
generated Appendix A,
by the acute respectively.
toxicity test are One hundred percent
survival average
occurred in the control wet weight (blotted dry)
exposure. of 0.0002 g
Control daphnids had an at the end of the test.
Loading Exposure
rate of
during daphnids
the to
thteoxriecfietryencteesttoxiwcaasnt apsopdriouxmimdaotdeelcyyl0.0s1ulfga/Lt.e
resulted in a 48 hour LC50 and EC50 of 24.8 mg/L.
The presented
24 in
and Table
48 3.
hour The
tEeCs5t0ssubfsotrandcaephwnaisdsnonetxopxoiscedtotodapCh-n7i8d7saraet
tested concentrations, FC-781.
resulting
in
a
48
hour EC50 of > 1,000 ng/L
EnviroSystems Page 10 of 16
Study
Number
9070-3
ResouceAnais inc.Subsidiary ofMULPORE 002636
Table 2. Survival data from toxicity test
EEE Nominal Concentration E(EngE/L) rep.
_-- Number Alive Ohr 24br 48 hr
-- Number Affected Ohr. 24h 4shr
0 (control) 1 2
10 10 10 10 10 10
0
0
0
0
0
100
1
10 10 10
2 10 10
9
0
0
0
0
0
0
150
1 2
10 10 10 10 10 10
0 0
0 0
250
1 2
10 10 10 10 10 10
0
0
0
0
0
400
1 2
10 10 10
10 10
9
0
0
0
0
0
0
600
1 2
10 10 10 10 10 10
0
0
0
0
0
0
W-- oteP: EEN-- o draphr ni-- ds were -- immobilizT edE(TunablT e to swimI within 1T 5 seconds after gentle agitation)
PEanvgiero1S1ysotfea1s6 Study Number 9070-3
ResourceAnche Inc.Subskiaryof MLLPORE 00263"
Table 3. Median effective concentrations (EC50s) from toxicity test
Exposure period
EC50
95 percent confidence limits
Calculation method
------------------
24 hours > 1,000 mg/L
-
-
48 hours > 1,000 mg/L
-
--
EEE
PEnavgiero1S2ysotfem1s6 Study Number 9070-3
Resource Ancis nc, Subsiaryof MUPORE 002638
1
:
x. nermmces
oDDiuAhdRe.ratinery19h8Fe1r".actsiDeoensc.issaioe5nn0o1f)ofth3echCeHnolimemuet)ts:.CoRncuarmnei3zr1g t`heORNDatpursilaciAsciceesptaonfceSooodf oRSrheeorott.iocincts1lp9oa8nt4,eTonistG..d"SdadltonhpeoesesdfJo`3ro00T,cesDt6einpgTaIof.sCpheesiAecrultse. TeSseocbtii1oinsa2t:ionStfTeecstts1onnd FStoerpthoamns,] coCm.mF.intb1a9t8i3o.n.Computer progres for calculation of 160 values.
SRurizeosyaetons Study esses 3070-3
avonsbe SonTE
|
o
BN
002639
Aependix A. ATERQUALITYDATAPRONTOXICITYTEST
EPnavgiero1s0ysotfen1s6 Study Nusber 070-3
Buscorioe,Ss VALROSE
:
002641
BN
Table A.1. CCoonndcuecnttirvaittiyo,n pRHe,ssutreemdperdautruirneg, taonxdicidtiyssotlevsetd oxygen
--_ conNcoeanttaraaltion
Cooonndducetivituy (e uahoe /ca)
TM uw
be br Be
Tan) Rep. de be br
k
feontrol) 12 1n3o0 110000 1130000 C811 8s0o 8sdi
130
13 am0o 11000 1122000 sLoo o#w1 8833
Bi
0
21 0Do0o 11000 11220000 ssoo sm1i 8833
i
w
13 m1o0o0 11000 11220000 snoo sm1i 883)
00
13 wnoeo 11000 112000 sLoo m811 8803
1,000
13 pmeeo 110000 11220000 ssoo 8822 83
'
--
EPangveiro1s8ysotfen1s6 Study Nusber 9070-3
ceArie L4G ANKLES 002641
Table AL. Continued.
.
_-- Xontnal
Tesperature (0r) ss Do issolvr ed Oxye gene(m tnolL)
concweantlr)ation rep. br bue 3uw b o b2 a
Er
0 (controll 12 2200.09 2w0o.0 1w9i.s8 C8e5s s9.o0 9as
1350
210a95 2w0o0 210a a8ss ssoo eaa7
0
21s 0s 220000 1 193 aass s5o0 aead
L
"00
212039 22000 w1i.s9 s8s5 ssloo 8e3a
500
219 0 22000 1189s sess ssoo saa3
1,000
2L920520 2 2a000 8833 9s0o ssoo
5
_--
Ek
EPangveiro1s6ysotfen1s6 Study Nusber 9070-3
Fusco ayes be,soncvmir
00264
