Document 2RG99EmXXrpgbyB3o6GOM84Lr

AR226-2839 | {-- o HLO 334) | BIOASSAY REPORT vg | SUBMITTED TO 2 : E:T. DUPONT DE. NEMOURS & COMPANY 5 ee q ; -_ k : ils =__ ' AHH i by &. ie Ll . : Tr| #2 ii 2 | g z it i Acie' toxicity of 8231 to rainbow 1 tr|o(aulnte' gairdneri). i i 7s5i0orifoaniincs,StrIenee.t } | JWuanree,ham1,973`Wassachusette . i < i a | ge 4 e METHODS AND WATERTALS | 3 "3 i This investigation as performed at ne duatic toxicology Fi Breton) fonSd te weve s snaitnd 58 E2 B &i laboratory of susceptibility Boifon`ormaiitibso,E y (tIrnoc.uA tWa(rSehhinansmiea:i)rdnMaesrssa)chusetts. ~The. vHoeis PHBL ofvalmen tron fea J. 50 Sap Ai )J-FoEa] (a in light terns yellow of the mlzieqduiiiadent,es8 tteelderlaneceiTinit PY) vas measured (1m), the nomipal HiTae Ce ; Ygwii= concentration. of tN heLchRenRDR S2A2R{E5 3A 4% wien SOS He aes a causes 50 percaepanpt 5 ry tad A 5i time interval, S The pred gine oe eg aluaee,d and itySTE9.5% nhap Ee a| confidence Interval. ess sedan soifersion of the concentrations'| `tested and. -the.cortS espaR ndinE t obseErvRed pecs ot portal iti:es, tail THCu eo34 fo 2 logs and probits eareulation ats Fespecltivaely. sit the nu equent Lincar Festi en prith. `we moamtlheeimyaytoincal od i of thechemical'tested waslevalustedunder: static bioassay: Sa fBz e conditions for a 96-ChouRrpePriod. rie0n Ar FE a] :|= he tes.tprodsaieSgeS afnml eeHSR he eS ls ars in' coimpleAte' oSaSTSEd E 0 acsondancs wish the Hessen neocon oe 1970 EB edition of Standard Nethads (APA) Lz those conditions | 58 described below. . diay CE El FE | Ee ; Se Ta5 Jd i | L-3 i|ii Com2panySmite. Gas -temntn TSCACE] i. : | a ? ':Fa^':'i^:S:^;ijij| The rainbow trout:..used: ^.R. t^tes:e..^&tsyi^'e obtained from a commercial fish ha'tpl'i^^'iTij'.lVia'ss^^'^e't^ and had a mean weight of 1.3 g ^nA-a^s.aTi..leng;th[^)^^@^im, The test population waB.he^-^^i^e^a^c^^b^^tia^chery:'& ' . ..;-. .^X^a^^^lS^'-'^M^^::,^ . .'.' ..^ ; .': for at .least 30i;dp^|^i^||^;||^g^y,.^ period .raortaliAy^.w^sQli^I^'i^it?s^iS^ffccff^i^^l^^aHss^ wer.&^e^nduiyted i.n 5, -gatloB g^ai^^s^i!^;N^^:^i3ffl^aE^?j3i|@^t ^. ; '''iK;w^:l.ff^l%|^^i&^^ .water ba.ths'atfI^,ta^^^^^|^l^|^ ,.^^::^^. temperature. ^ ': :.,"':'i' ';' 'i: n^ gerarte.d., , ,na /into -the, A;e;st::.weililI^il|^|e8|3'^i^^.c^^ ' grea'-tes-t^ampfln'l;:!!!?!^^! ^S^SB^^^IIftSI?^1"^^^^^'^ ..--^^^^ ^Mll^ ^^ fl^;a|^|I|| "^iw each .a?e!st^el^SIi lifelilta^iplie^^er^sei^w^ ^%iSIS|fl -'^'-' "r"" ^^^^S^-^-"--^ .....t:e s t; '.sys:t effl-wa.^lp^epN'i aiflga^aMg?^eAll:dm;^^ -; .;^ ^ .-^ --^i^^^'-^s^^^gg^gijgis^ji^:.^'^ ^fill:- ^to d^ip'p^z^d^y^ti^ilNlSIISI^^ .Hardness :^^^^|Tig^c>MysaS^^ ?jg|g|^|^^-, 30 The pH of'the-s||n|aK|s^tiina^^ orange alkalinity:iWa^;;^|5^;ppnT;;(a&^ oxygen values for the va^ipus^es^vesseai^^^^ from 8.6 initially to 5. a mg/1 ,a t t^erid-^^ith^fi-tests. y|,anySanni^.^-c^^CAC^: 1 = gs CR !; : RESULTS [| os wirbeEn il nie =3 3 ---- E 3 rad RA bt mets e+e | E The preateted mig) tie isith torrets SA presented in ee summary of observed | ii i ks= HmoorritbaulnidtyffioshrS gtehnea'hralilE yNoeeasafmteedGrE a2rsbasnnday96lethhoaurrgsic,oflorpsotnneseoe Ro E 3 cuitiveson, srdidopid Ti : Sagan 0sa +E Fe : E e oq = %, ChaThRiEnNe BSueRnRa ooRE SrSIC: o i SEFnS EciahE yS eeE lE s vo Uw a a ia e r 2& E iae e . 15 aseR PTI RR ne, i ie SA B5 = FSe oha eh te CA[ atT= a| 2 pa Conia @ hoy denon of Se i Sebi to I 5 CompanySanitze.Doss notcons TSCACBI | bea Sead y SUBMITTED BY; Bionomics, Inc. 79P Main Street Wareham, Massachusetts June, 1973 i-- PREPARED BY APPROVED BY: Company Sanitized. Does 1106 contain TSCA CBi Company Sanitized. Does not contain TSCA CBI Table 2 -- Concentrations of 8231 tested and corresponding obs3erv<ed | percent mortality for rainbow trout (salrno gairdner^i) at 2^ and 96 houirs during a static M oassay. Y^ Compound 8231 ^^^^tgQ Conce tiltration.^.' (m ;.,'.''^,. ':: ^750;-: ;560;; ' ^ ---"'- '--'. "',-"\",",'' ;. ^oC ^',:;^-':'.:^^^.^|;^ S-S^^^^iS^f'iil ^fsapj '-''"' -;""'' '.:':"'"'" :'.'i'':y'^":' ^' , ' \ ^$46^:^^:,^;^-:;^m '^^^oo^ :'^::;;;i::^'':^^c;sie^ /'.^^^a]!^ ' -' i'--''' '. ''.^ ^t^"'-:^1^';^ ^:::'^'?7'} '^^;8^'::: y:/, ;;/ . ;7^;^1 ^:^^ .:;.-^ .^^ .:':" ;':';';i.^. ^^ ^^'^"''S'i?^ W^.':^, ^ ^^^i^y^?^':^ ^^CflS^O^:^^^^^!^ - -.^^corirt|- o^^ajsoprlag^^ileo 1) \? .. '."':. /^.j: ..- ,;.^^^;:,^..-;-; ^C^^'L.'^W?-^''; a^F.ish exhibited .loss ^of^eqai^iibra.um^^^.^ % mortality 24 hour 100 observed'. ^ 96 "iioia^ ' " -\ .'"-'-< ..: .^,.'A*j ' :' .' " '"":". l.1-;' ^ :. ' ^'^3 100 '1 :;| ..: -:-a 90 100 . 60 100 M '-f" .-> . ' :' '-,";! 0 . . 0 lOO^.ati . -""."/ ^, -:-".'.- y-~^ loQ^yH .. v '-": .'^---^.sC'fej 0 /,a?.'^s|a 0 0 0 ; .0 ::-;-- - "\-; ^'i ,;;^^"si 0" ^^;^'^S|g i n .."^ - ^'^B ".^.J^.'"'':^^ ' '.^ -m Company Sanltizsd. Doss not contain TSCA CB(