Document 2R5Eorxp8N5y9y769yO6Qzmr6

^ 216-0 5 /^ ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid; may also be referred to as PFOA, FC26, or FX-1001. (Octanoic add, pentadecafluoro-, CAS # 33567-1) Remarks: The 3M production lot number was 269. The test substance is a white powder. The test sample is FC-26 referred to by the test laboratory as N2803-3. The purity of the sample was not sufficiently characterized, although current information indicates it is a mixture of 96.5 -100% test substance and 0 - 3.5% C6, C7, and Cg perfluoro homologue compounds. - Remarks field: The sample preparation directions given to the laboratory were to dissolve the test material in a 50:50 waterisopropanol solution. In the protocol amendment, it was stated that the sample was combined with isopropanol in a 50:50 ratio prior to use. METHOD Method: U.S. EPA-TSCA Guideline 797.1400 Type: Static acute GLP: Yes Year study conducted: 1995 Year study completed: 1996. Species: Pimephales promelas Supplier: Aquatic Biosystems, Fort Collins, CO Analytical monitoring: DO, conductivity, pH and temperature were monitored daily. Exposure period: 96-hours Statistical methods: LC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non-linear interpolation using the computer software of C.E. Stephan. Test fish age: Not given. Length and weight offish in control: Average total length = 33.4 mm, average wet weight = 0.35 g. Loading: 0.23 g fish/L Pretreatment: None Test conditions: Dilution Water: Deionized water adjusted to a hardness of 40-48 mg/L as CaCOs/L C03964 Dilution water chemistry: Representative sample given in Table 1 of the final report. Temperature: 22 + 2C Lighting: Cool-white fluorescent bulbs with an intensity of 31 ft-c. Photoperiod of 16-hours light, 8-hours dark with a 15 minute transition period. Stock and test solutions preparation: Appropriate amounts of test substance were added directly to the dilution water in the test vessels. No insoluble material was noted during the test. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted. Exposure vessels: 20 L glass aquaria containing approximately 15 L of test solution, water depth approximately 18 cm. Number of replicates: two Number of fish-per replicate: ten Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 6.4 - 8.7 mg/L (control exposure) 5.2 - 8.8 mg/L (220 mg/L exposure) Conductivity range (0 - 48 hours) 120 pmhos/cm (control exposure) 110 pmhos/cm (220 mg/L exposure) pH range (0 - 96 hours) 7.1 - 7 .6 (control exposure) 6.2 - 7.2 (220 mg/L exposure) Test temperature range (0 - 96 hours) 21.9 - 22.6C (control exposure) 21.9 - 22.6C (220 mg/L exposure) Remarks: The pH of the test solutions for the 360, 600 and 1,000 mg/L exposure concentrations were in the range of 3.0-4.3 at test initiation. This low pH would have adversely affected the survival of the fathead minnow. RESULTS Nominal concentrations: Blank control, 130, 220, 360, 600,1,000 mg/L (Tested as 50:50 mixture of test substance and isopropanol). The concentration of test substance in solution was 65, 110, 180, 300 and 500 mg/L. Element value (as-tested): 24-hour LCso = 280 (220 - 360) mg/L 48-hour LCso = 280 (220 - 360) mg/L 72-hour LCso = 280 (220 - 360) mg/L 003965 96-hour LCso = 280 (220 - 360) mg/L 96-hour NOEC = 220 mg/L Element value (based on concentration of test substance in solution): 24-hour LC50 = 140 (110 -180) mg/L 48-hour LC50 = 140 (110 -180) mg/L 72-hour LCso = 140 (110 -180) mg/L 96-hour LCso = 140 (110 -180) mg/L 96-hour NOEC= 110 mg/L All element values based on nominal concentrations. A toxicity test conducted previously with isopropanol at 500 mg/L showed no mortality or sublethal effects. Statistical Evaluation of Mortality: All of the LCso values were determined by binomial interpolation. Biological observations after 96-hours: Fish in the control and the 130, and 220 mg/L exposure concentrations appeared normal. Total mortality was observed by 24 hours in the 360, 600 and 1,000 mg/L exposure concentrations. Cumulative percent mortality: Nominal AsTested Cone., mg/L 24-hours 48-hours 72-hours 96-hours Control 0 0 0 0 130 0 0 0 0 220 0 0 0 0 360 100 100 100 100 600 100 100 100 100 1,000 100 100 100 100 Lowest concentration causing 100% mortality: 360 mg/L Mortality of controls: None CONCLUSIONS The as-tested test substance 96-hour LCso for fathead minnow was determined to be 280 mg/L with a 95% confidence interval of 220 - 360 mg/L. The as-tested test substance 96-hour no observed effect concentration (NOEC) was 220 mg/L. The 96-hour LC50 for fathead minnow based on the concentration of test substance in solution was determined to be 140 mg/L with a 95% confidence interval of 110 - 180 mg/L. The 96-hour no observed effect concentration (NOEC) for the test substance in solution was 110 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Additionally, there appears to be a discrepancy between the sample preparation directions given to the laboratory and the procedure conducted by the laboratory to prepare the test solutions. The absence of partial mortality at intermediate doses resulted in the sharp dose-response curve seen in this study. As a result, the LC5o values determined in this study may not accurately reflect the true toxicity of the solution. The low pH values in the high concentrations tested may have had an adverse affect on survival. REFERENCES This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company. OTHER Last changed: 5/25/00 C-03967 BEST COPY AVAii ABLE i BEST COPY AVAUABLE I. GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT This study was conducted according to e p a Good Laboratory Practice Regulation (40 CPR 792), with the following exception. The atability o f ' the teat substance under test conditions was assumed but not verified at T.R. wilbury Laboratories. Deviations from the study protocol and protocol amendments are presented in Section XII of this report. KST COPYm u m II. QUJLLXTX JUSURAXCS STATtXSMT i* 'p-jH /ff*. Submitted by i T.R. Hilbury Laboratori, Inc 40 Doaks Lan Harblehaad, Kaaaachuaatta TS certification! ifcpe. T.R. Hilbury Laboratoriaa, Inc. atudy number 891-t h , 1 p,V Acuta Toxicity of N2803-3 to the Fathead Minnow, Plmaphalaa pronai, waa audited by theQuality '<$>'&> Aaaurance Unit for compliance with the protocol, standard operating procedures, and applicable Good !JI. Laboratory practices (U.S. EPA, 1993). Quality assurance audita vara performed and the findings "i reported to T.R. Hilbury Laboratories management and the "'.it study director on the following dates: Aspect Audited Protocol: In-life i Draft Report: Pinal Report: Audit Date 8/ 4/95 9/ 5/95 9/13/95 1/24/96 Reported to Study Director 8/ 4/95 / 5/95 9/13/95 1/24/96 Reported to Manageaient 8/ 4/95 1/24/96 1/24/96 1/24/96 _ '' 'TvjSff BEST COPY AYAi! ABLE III. 7ABLB or COKmiTi . . . T^-v}.. <', * st. ; / /,% . \ ** ,3>Vit?; ?, .,1 /'f: I. Good Laboratory Practice Compliance Statement 2 ZI. Quality Aaaurance statement 3 ZIZ. Table of Contenta .'zv. ` Index pf Tables and Figures . .4 ''.y; ..... '5 * V. Summary 6 VI. General Information 7 VII. Introduction ..'- ' 8 VIII. Methods and Materials 8 IX. Results 13 X. References XI. Signature Page .* . ' . 18 '/\^ 19 XII. Protocol Amendments and Deviations . . v< ' .4\' ^ "V . ' 20 -i .V. i,- Appendix A. Water Quality Data from Toxicity Test 'T:(- * BEST COPY AVAU snow n V. UMHJUtI The acuta toxicity oi N2803-3 to tha fathead minnow, Pimmpbmlaa proawlas, ia daacribad in this final raport. Tha taat waa conductad for 3M Company for 36 houra from Saptambar 1 to S, 1995, at T.R. Hilbury Laboratoriaa, Inc. in Marblahaad, Kaaaachuaatta. It waa conductad by Jaanna Magazu, Jacqueline Maviua, Tatar Kowalski, Xeven Stavana, Tatar Naviua, Robert Boari, and Timothy Hard according to tha protocol developed for T.R. Hilbury Study Number S91-TB. N2803-3 was supplied by tha sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature Of 21.8 to 22.6*C. Tha dilution water was filtered deionized water collected a t Marblehead, Massachusetts and adjusted to a hardness of 40-48 mg/L as CaCO}. Aeration waa initiated at 72 hours to maintain dissolved oxygen concentrations above an acceptable level. The sample of N2803-3 supplied by tha sponsor was combined with isopropanol in a 50:50 ratio prior to use, and this mixture is defined as the test substance as-tested. A toxicity test was also conducted with 500 mg/L isopropanol and no mortality or sublethal effects were observed. Results of the toxicity test are reported twice, onca based on nominal concentrations of test substance as-tested and a second time based on concentrations of test substance as-received (N2803-3 without isopropanol). Nominal concentrations of N2803-3 as-tested wera 0 (control), 130, 220, 360, 600, and 1,000 mg/L and nominal concentrations of N2803-3 as-received were 0 (control), 65, 110, 180, 300, and 500 mg/L' and nominal. Fathead minnows used in the test were procured from a commercial supplier and acclimated to test conditions at T.R. Hilbury Laboratories. After 96 hours of exposure the control fish had an average wet weight (blotted dry) of 0.35 g and an average total length of 33.4 mo. All fish were in good condition at the beginning of the study. V Exposure of fathead minnows to the test substance resulted in a 96 hour median lethal concentration (LC50) of 280 mg/L N280J3-3, with a 95% confidence interval of 220 to 360 mg/L and a 96 hour no observed effect concentration (NOEC) of 220 mg/L, based on test substance as-tested (50t N2803-3 and 50% isopropanol) .' The 96.hour LC50 is 140 mg/L N2803-3, with a 95% confidence interval of 110 to^lSO mg/L and the 96 hour NOEC is 110 mg/L, based on -testsubstance.^ as-received (N2803-3 without isopropanol). ^ ' '' '-ft 'S' j BESTCOPY AVAHABLE BEST COPY AVAII ABLE f . rtci- v u INTRODUCTION This study was sponsorsd by 3M company, St Paul, Minnasota. Tba objactiva of tha study was to determine tha 24, 48, 72, and 96 hour median latbal concantrations (LCSOs) of tha tast substanca to tha fathaad minnow, Pimph*l*a promala, a frashwatar fish, undar static conditions. Tha tast protocol was basad on U.s. e p a mathods (U.s. e p a , 1993). Tha raport contains sections that describa tha mathods and materials employed in tha study, and results of the investigation. Tha raport also contains an appendix that presents water quality data collected during tha teat. VIII. KBT80DS AND MATERIALS TEST SUBSTANCE I Tha sample of N2803-3 (T.R. Hi1bury Laboratories Sample Number 530) was delivered to T.R. Wilbury Laboratories on August 29, 1995 in a 5 gallon metal bucket within a cardboard box at ambient temperatura. It was contained in an approximately 200 ml glass jar. The label attached to the bottle included tha following information! "N2803-3, s. Beach, 2-3E09, (612) 778-7452, 8/22/95." N2803-3 (a white solid) was supplied by 3M Company, St Paul, Minnesota. Prior to use tha sample of N2803-3 was stored in the dark at room temperature. Tha sample of N2803-3 supplied by the sponsor was combined with isopropanol in a 50:50 ratio prior to usa. This 50:50 mixture was then considered to be 100% test substance during tha performance of the toxicity test. All results axe reported both on tha basis of test substanca as-testad (50% N2803-3 and 50% isopropanol) and test substance as-recaivad (N2803-3 without isopropanol). The N2803-3 was assumed to have a purity of 100% active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned to the sponsor. DILUTION WATER: water used for acclimation of test organisms and for all toxicity testing was 'deionized water collected at T.R. Wilbury Laboratories.in Marblehead, Massachusetts . Water was adjusted to a hardness of 40-48 mg/L cacOj and stored in 500-gallon polyethylene tanks where it'was aerated ! continuously passed through a particle filter, ultraviolet sterilizer, BEST COPY AVARABLE . '.' Tabi* 1. chamical charactariiation oi a rapraaantatlva aampla of dilution watar uaad for tha toxicity taat with fathaad minnowa, PiaaphMlta proaalaa, and N2803-3. Paramater1 Unit of ' Maaauramant Dataction : Limit 'Maaaurad 'valua ; ?' ; ' Matala Aluminum Arsanic Boron Cadmium chromium cobalt coppar Iron Load Marcury Nickal Silvar Zinc r .A* ^ \;* . ; Hitrata Chlorida -Pluorida Total Organic .carbon ' Total Phoaphorus Organochlorino Paaticidaa Toxaphana mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L aa N mg/L mg/L mg/L mg/L pg/L pg/L 0.1 0.01 o.s , 0.0002 0.01 0.03 0.005 0.03 0.005 0.0003 0.03 0.02 0.02 0 HD* HD HD no : ND HD HD 0.03 HD m> HD HD ..-HD 0.05 1 0.1 HD ; HD 1 0.03 t HO *... 1 0.5 2 "" HD HD- * 1'V BEST COPY AVAIl ABLE '.--SR prior to tasting tha fish wars maintained under flow through conditions in a 270 liter fiberglass tank. During acclimation fish were not treated for disease, they were free of apparent sickness, injuries, and abnormalities at the beginning of the test, and mortality during tha final 49 hours of acclimation was less than 3t. During the 14 day period before tha start of the test the acclimation temperature range was 21.0 to 22.3*C and the dissolved oxygen concentration was always at least 9.0 mg/L. During acclimation fish were fed a dry commercial food (Tetra Min* staple Food, Lot # TM05) daily except during the 49 hours immediately preceding the test. Fish were not fed during the test. At the conclusion of tha test the control fish had an average total length of 33.4 mm and an average wet weight of 0.35 g (loading during the definitive toxicity test was 0.23 g/L). TOXICIT* TESTING: A static screening test was conducted from August 19 t o '22,' 1995 with five concentrations of test substance as-received and a dilution water control. Nominal concentrations of N2903-3 were 0.05, 0.50, 5.0, 50, and 500 mg/L. After 96 hours of exposure there was 100* survival .n' the control, 90 survival at 0.05 mg/L, 100 survival at 0.50, 5.0, and 50 mg/L, and 0 survival at 500 mg/L. % >, * The definitive toxicity test was performed from September 1 to 5, 1995, according to T.R. Wilbury Protocol 991-TH (Acute Toxicity of N2903-3 To the Fathead Minnow, Plmephalaa promelas). ' The protocol, which was signed by the study director on August 16, 1995, is based on procedures of the u.s. Environmental Protection Agency (1993). A summary of the test system is presented in Table 2. ;V . The test was conducted at a target temperature of 22 2*C with five 5 concentrations of test substance and a dilution water, control.'-. Nominal ` .^concentrations of the test substance as-tested were:; 0. mg/L (control) , 130, 220, 360, 600, and 1,000 mg/L. Appropriate amounts of test substance. </; wsre added directly to dilution water in test vessels to formulate'test ./"' media. 'S.U. v ....... . > > " -..-?*<.:}'-3&. V among two replicates of each treatment. The itest-'Wasi'perfbrmed'^in^ i20 liter glass aguaria that contained 15 litersof test';BolutionJ;(wateri rdepth was.approximately 19 cm) . Test vessels were randomlyarranged'&nva^ water bath .during the 96 hour test.(a 'random ;numbers'|;tabl<^wa's|used_|io^ iselect the,location of-each vessel)and., theywere |:lbbsslytcweri ^>>w;Xlght^ii^;^i^'gjdagk''phctopegi^,w:lth'..,a ^ ^ i & u i ^ ^ ^ S S 8 i s s^automatlcaliySmainta'inedl-'withi^ct^l-^hitis^luore^ent ovidedialillghtvintensity,of i;3l|footcahdlea.!^?Aeration'S ^ourlaltsolvd^oxy9xi^concentration^aasurttic gen"concentrations vabove^acceptabli?ilevi|l 3977 BESTCOPY AViUi ABLE 1 ' K"'*:S'-i * - ,v i ' V 11. :A > - ---; v : f i . is v k : * i f J h f c y c , ' i-. + y k ' r ' V i " ' ^ '.<! . , : 4 * & f c " ?' S 'V T ^ iiim 'i'V5! , ,'-V1's * t. v < '3 BEST COPfAVAll ABLE The number of surviving organisms and tha occurrence of sublethal affacta (immobilization, losa of equilibrium, losa of .reflex, 'k'. axeitability, discoloration, or changa in bahavior) vara dataminad ! visually and racordad initially and aftar 24, 48, 72, and 9166 hours X -; , (mortality and sublathal affacts vara monitored tvica aach day)l-r tast organisms vara removed when first observed. Dissolved oxygen (YSI V- Modal 57 mater; instrusmnt number 1), pH (Beckman modal pHl 129 matar; instrument number 124), conductivity (cole Parmer mater, instrument number 94), and temperature (Beckman modal pHI 12 meter; instrument number 124,' ,v*.^ proba pBT-4) vara measured and recorded daily in each test chamber that contained liva animals. The temperature in one test vessel vaa recorded at least hourly during tha tast. A static test vaa conducted from September 29 to October 3, vith 500 mg/L Isopropanol (7.5 g isopropanol added to 15 liters of dilution vater) and a dilution water control. The test vas established vith 2 replicates of aach concentration and 10 fish per replicate, and it vas conducted in a manner similar to the test with M2803-3, Test vessels vara randomly arranged in a vater bath and the light intensity .was 28 footcandleB. I. STATISTICAL METHODS I Results of the toxicity test were interpreted by statistical techniques. Computer methods (Stephan, 1983) were! used to'' calculate the median lethal concentrations (LC50s). All calculatior.sverej performed using nominal concentrations of test substance as tested and'^the'j number of live fathead minnows. The no observed effect concentration is: ;the highest concentration of test substance that did not causa ,: toxicant-related mortality or sublethal effects. ' r,r'is4i; 'v '***^ ^ *\ 41 , 1",1 0.3979 EStOffUV&t ix. results Insoluble material was not noted during the teat. Biological and water quality data generated by the acute toxicity test are presented in Table 3 and 4 and Appendix A, respectively. The measured concentrations of dissolved oxygen ranged from 5.2 to 9.0 mg/L, the temperature ranged from 21.8 to 22.S*C, conductivity ranged from 110 to 370 pmhos/cm, and pH ranged from 3.0 to 7.6 (conductivity and pH rare affected by the test substance).' One hundred percent survival occurred in the control exposure and these fathead minnows did not exhibit any sublethal effects. During the toxicity test with 500 mg/L isopropanol no mortality or sublethal effects were observed and the 96 hour I.C50 value is greater than 500 mg/L. The 24,"48, 72, and 96 hour LC50s for fathead minnows exposed to the test substance are presented in Table 5. The 96 hour LC50 for fathead minnows exposed to the test substance is 280 mg/L, with a 95% confidence interval of 220 to 360 mg/L and the 96 hour NOEC is 220 mg/L, based on test substance as-tested (50% N2803-3 and 50% isopropanol). The 96 hour LC50 is 140 mg/L, with a 95% confidence interval of 110 to 180 mg/L and the 96 hour NOEC is 110 mg/L, based on test substance as-received (N2B03-3 without isopropanol). The slope of the 96 hour dose response curve could not be determined with these data. BESTCOPYAVA ABLE Tabla 3, survival and aublathal affact data fro tha toxleity taat with fathaad ninnova, Piasphalas proaalaa, and N2B03-3. Nominal Concentration of N-2S03-3 (Bg/L) aa-Teeted as-Received Rep. Number Alive 0 24 48 72 96 hr hr hr hr hr Number Affected 0 24 48 72 96 hr hr hr hr hr n" 0 (control) l 10 10 10 10 10 2 10 10 10 10 10 00000 0 0 0 0 0 :; 130 65 220 110 i 10 10 10 10 10 2 10 10 10 10 10 1 10 10 10 10 10 2 10 10 10 10 10 00 00 0 00000 00000 00000 V'5* Mi 360 180 '.'i'' ' 10 0 0 0 0 0 2 10 0 0 0 0 0 600 300 1 10 0 0 0 0 2 10 0 0 0 0 6 7 f vr^: : ti.Vi. - 1,000 500 r"-:'JTrb-'J-t-r*->a<'-*>a,,??< t, 1 2 \ 10 0 0 0 0 10 0 0 0 0 0 c-- --- -V ' t'i { Notes*j l.'v;All of the fish exposed to 1,000 mg/L test substance as-tested ~<'i* ..died immediately and 7 of the 20 fish exposed to 600 mg/X. teBt "'i'l ; substance as-tested died immediately. ' - 2. Affected fiah were swimming in short, irregular burats and J`v, ,r'"S ' '** than floated motionless to the bottom of the teat vessels, i';'1?*' "4 i*' *,st > .v- *7 .. V - * V '' 1 v .*r 'v . /- e c ,.'.nh'*'- 1 V 1 . ft' *' 5\-. :v'<ifjf*1 039S1 B tS T W ffV W IM U B L t Tabl* 4, Survival and aublathal aifact data iron tha toxicity taat with fathaad minnows, Plmsphales promalas, and isopropanol. ; Nominal Concantration of Xsopropanol (g/L) Numbar Alive 0 24 48 72 96 Rap. hr hr hr hr hr Numbar Affactad 0 24 48 72 96 hr hr hr hr hr r > %. 0 (control) 500 . . 1 . 10 10 10 10 10 2 10 10 10 10 10 . 1 10 10 10 10 10 2 10 10 10 10 10 00000 00000 00000 00000 `i . 'fi* T'lP'" *i KSTCOPHM KST COPYAVM ABLE Tabi 5. Madian lathal concentration (LCSOs) fron th toxicity teat with fathead minnow, Pimephales prom!, and H2803-3. Exposure period 1X50 95 percent confidence limit Calculation siethod Calculated based on Test Substance as-Zested (50% M2B03-3 and SOI Isopropanol) 24 hours 280 mg/L 220 to 360 mg/L Binomial 48 hours 280 mg/L 220 to 360 mg/L Binomial 72 hours 280 mg/L 220 to 360 mg/L Binomial 96 hours 280 mg/L 220 to 360 mg/L - Binomial Calculated baaed on Test Substance as-Received (H2803-3 Without Isopropanol) 24 hours 48 hours 140 mg/L 140 mg/L 110 to 180 mg/L 110 to 180 mg/L ' ' t s' Jt , Binomial t'. i i' > " ' '^ ^ * t_ "* , Binomial . 72 hours : 140 mg/L 96 hoar v ^ . 140 mg/L 110 to 180 mg/L 110 to 180 mg/L f* Binomial ' ~ *? -- 1 vBinwnial^^*' it >. 1A** > ;>4 .> ' ' ^ ; v ,rs.i(', * h ? k :: - n m r i 00393 -V , ' -' >' y V >'< '**/ BESTeopy AVAIIABLE XX. BXOKATUK* PAOI \ t 4 .^^ !U m L. Kovalaki . ic Toxicologiat , 's BESTCOPY AV ABLE XII. PROTOCOL M U M D I T I IUR> DEVIATIONS '- PROTOCOL AMENDMENTS I 3T .T Th protocol was amended to specify that th test subatanca would ba comprised of 50% N2803-3 and 50% isopropanol, to note tha nominal concentrations for tha definitive test, and to note that an additional toxicity test would ba conducted with isopropanol. % PROTOCOL DEVIATIONS! Dissolved oxygen concentrations were <60% saturation at 72 hr prior to aeration. Teat vessels were aerated prior to sponsor approval. : The slope of the dose response curve was not provided. In the isopropanol test,;not all fish were between 0.1 and 1.0 g. These' deviations did not affect the outcome of the study and no o t h e r deviations were made from the protocol. >-w ?;" \ *.? ' - ' - ' , ; \ - * . , s' i* *i **: "1 V\, ` Vf,.~-ia'i,y * lit **V> f* - r 4 ,,- *' >if ' ' ' rj>K'- . Vw^ , ^ >hi * -C *-/ M "*r '" j . ;,. v . . - , ' - V ' . C039S7 BESTCOPYAVAIisBlL BESTCOPYAYAHABLE Table A.l. Diaaolvad oxygan concantration, conductivity, pH, and tanparatura aeaaured during the toxicity taat with fathead ainnowa, Riaephalea prooaiaa, and N2B03-3.- Nominal concantration Oi N2803-3 aa-Teated (mg/L) Rep. Diaaolvad oxygan (mg/L) ------------------------ 0 24 48 72 96 hr hr hr hr hr . conductivity (mhoa/cm) ---------------------- 0 24 48 72 96 hr hr hr hr hr 0 (control) 1 2 130 -- 1 2 220 1 2 360 1 2 600 1 2 1,000 1 2 8.6 7.5 6.5 6.5 7.4 8.7 7.4 6.5 6.4 7.5 8.7 7.5 6.5 5.8 7.5 8.9 7.5 6.5 5.5 7.5 8.7 7.4 6.5 5.2 7.2 8.8 7.5 6.5 5.3 7.2 8.7 8.0 8.7 8.0 8.9 8.0 8.9 8.1 9.0 9.0 * 120 120 110 110 120 120 120 110 120 120 110 110 110 120 120 110 110 110 120 120 110 110 110 120 110 110 110 110 120 110 120 120 120 120 180 200 200 200 350 370 BESTCOPY1AVAHAME Tabla A.l. continuad. Moninal Concantration aa-Taatad (m g / z.) Rap. 0 24 hr hr pH 48 72 96 hr hr hr Taoparatura (c> 0 24 48 72 96 hr hr hr hr hr 0 (control) 1 2 130 1 --2 220 .... 1 2 360 1 2 600 1 2 1,000 1 2 7 . 6 7 . 3 7.2 7 . 1 7 . 4 7 . 6 7 . 3 7.2 7 . 1 7 . 4 6.8 7 . 2 7.2 7 . 1 7 . 4 6.7 7 . 2 7.2 7 . 1 7 . 4 6.3 6 . 9 6.9 6 . 9 7 . 2 6.2 6.8 6.9 6 . 9 7 . 2 4.2 4. S 4.3 4.6 -- -- 3.4 3.5 3.4 3.4 -- -- -- 3.0 3.0 2 2 . 1 22.6 2 1 . 9 2 2 . 0 2 1 . 9 2 2 . 1 22.6 2 1 . 9 2 2 . 0 2 1 . 9 2 1 . 9 22.6 2 1 . 8 2 2 . 0 2 1 . 9 2 1 . 9 22.6 2 1 . 8 2 2 . 0 2 1 . 9 2 2 . 4 22.5 2 1 . 9 2 2 . 0 2 1 . 9 2 2 . 0 22.6 2 1 . 9 2 2 . 0 2 1 . 9 2 2 . 1 22.5 2 2 . 1 22.5 -- __ __ ---- 2 2 . 1 22.6 22.1 22.5 -- -- 22.5 22.5 - i "* , .''v . 'fl. * * %> - * 'V * . vi ^ .IfiS' ' >' s ' i 1 >'.V;t.'V .vSj<-.*4 'fltudy Munbar 891-TH ft?;' ." H 'V f / /; '. ` - -*, .rJ M M M I ? f;,: , rag. 23 * |p fi$Tcopy M il ABLE ^ si Table A.2. Diaaolved oxygen concentration, conductivity, pH, - and temperature meaaured during the toxicity teat with fathead ' minnova, Pimtphtltt proaelaa, and iaopropanol. W.v'v: nominal Diaaolved Oxygen (mg/L) of Iaopropanol 0 24 48 72 96 (mg/L) Rep. hr hr hr hr hr lconductivity (paihoa/cm) 0 24 40 72 hr hr hr hr hr -><`v. 0 (control) 500 1 2 1 2 8.6 7.8 7.6 7.1 6.2 8.6 8.0 7.6 7.4 6.8 8.6 8.1 7.8 > 6.2 5.5 8.6 8.0 7.6 6.3 5.6 140 160 160 160 140 160 160 160 160 140' 160 160 160 140 160 160 160 KST COPYAVANABLE