Document 2OpVqEYqgqXK5oBoqbYd8Nr

z-s nev 13 7* p E5T**USOi3Z E . 1. d u P o n t d e N e m o u r s & C o m p a n y IN C O H ^O R A fC D W i l m i n g t o n , D e l a w a r e 19898 POLYMER PRODUCTS DEPARTMENT experimental station ANALYTICAL REPORT PERSONAL AND CONFIDENTIAL cc: A. J. Dahl - 353 O'- -P. ThistLeton - Wash. Wks. PRAL File I.C. / V -J \ 3 5- / (> / January 23, 1981 TO: DR. Y. L. POWER - PPD, Washington Works FROM: S. S. STAFFORD >. ANALYSIS OF BLOOD SAMPLES FOR ANALYTICAL METHOD COMPARISON (JOB No. 800-598, 802-5500, 800-5518; PRAL Nos. 80-62910-922, 80-63834-847, ____________________ 80-64329-330, 80-66546)__________________________ Notebook Number: E22514 As requested in your letter of 10/2/80 to L. J. Papa, the 27 blood samples listed in the attached table have been analyzed for perfluorooctanoate (Cg). The results given there are for analysis by the Cg/GC method ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 1/2/81). The samples were also analyzed for organic fluoride by the Wickbold Torch procedure, and a comparison of the results and analytical methods is discussed in a separate letter being sent from L. J. Papa to Paul Thistieton (1/23/81). As noted in the table, repeat samples were requested in two cases where difficulty was encountered in the analysis. For PRAL No. 80-63847, results of the re-samples are reported here. For PRAL No. 80-63840, the analysis will be repeated when a second sample is received. Attachment fmt Key Words: Perfluorooctanoic acid Ammonium perfluorooctanoate Blood analysis GC There's a world of things weYe doing something about 000043 0 >-V V;? EXPOOOOOl EID 713815 V Sample PRAL No. 80-62910 80-62911 80-62912 80-62913 30-62914 80-62915 80-62916 80-62917 80-62918 60-62919 80-62920 80-62921 80-62922 80-63834 80-63835 80-63836 30-63837 80-63838 80-63839 80-63840 80-63841 80-63842 80-63843 80-63844 80-63845 80-63846 80-63847 80-64329 80-64330 REDACTED P. R. No. Date Sampled 8/20/80 8/20/80 8/20/80 8/20/80 8/19/80 3/19/80 8/13/80 8/19/80 8/18/80 8/19/80 8/18/80 8/20/80 8/19/80 8/29/80 9/3/80 9/3/80 8/29/00 8/20/80 8/21/80 8/25/80 8/29/80 8/29/80 9/2/80 8/26/80 8/29/80 8/26/80 8/21/80 9/J7/80 9/17/80 Name q ^ & GC Analysis Date Analyzed fCsl, pq F/q blood(lj) 9/2/80-9/5/80 aa aa aa mm Nm 13. 4.9 0.78 21. 6.4 0.015 aa n ma ma ma an ma 2.9 5.6 29. 7.8 1.2 0.26 4.6 9/9/80- 9/12/80 a 0.22 0.52 aa 0.40 9/15/80 -9/18/80 1 aa aa a aa aa am 1.9 24. 8.2 (c) 1.5 4.0 3.7 6.6 aa 0. 72 aa 5.5 ma (''-.02) 9/24/80 -9/26/80 a 0.022 (a) Analysis as described in the Cg/CC method, using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. Estimated uncertainity +10t relative standard deviation. Lcwer limit for quantitation 0.007 ug P/g. -- (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total fluorine analyses. (c) Unusually large variation was found in both GC and WicJdbold Torch results for repeat analyses of this sample (80-63840 as received, 80-66546 after lyophilizatlon of a portion), with values ranging from '>-26-41 ppm P. Sample inhoraogeneity may have contributed to this, but the reason for the problem is not really known. Analysis will be repeated on a second sample, as requested 11/25/80 by P. Thistleton. (d) In GC analysis of the initial sample (80-63847), three unusual peaks were observed in the region of interest, one of which interfered with quantitation of Cs at this low level. In the repeat samples (80-64329 and 30) these peaks were greatly reduced in size, and no interference with the Cg was apparent. The value given is the average for the two repeat samples. 000044 EXP000002 EID 713816