Document 2NbmJoM36NbbzEXdMYXrRE695

M 0802s ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206C and L-5439. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 501. The test sample is L-5439. Current information indicates it is a mixture of 0.73% PFOS, 76.09% water, 15% diethylene glycol butyl ether, 4% urea, 1.55% sodium octyl sulfate, 1.47% hydroxy foamer, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.. The following summary applies to a mixture with incompletely characterized concentration o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical compnent o f tee test sample.. METHOD:______________________________________________ . Method: 3M Protocol, Reference EAR 11/8/79 Test type: Static acute GLP: No Year Completed: 1981 Analytical monitoring: Dissolved oxygen concentrations Statistical methods: Graphed Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution water: Deionized water. Synthetic sewage: Not used Reference and test solution preparation: A stock solution of the test substance was created by diluting 0.60 grams of test sample to 100 mL with dilution water. It is not noted exactly how the 1,000 mg/L test solution is prepared from this stock solution. Test vessels: Not noted Number of concentrations: 1 plus 1 blank control Temperature: Not noted Total suspended solids and pH on day of testing: Not noted Element Basis: Respiration inhibition as determined by oxygen consumption. 001652 RESULTS Nominal concentrations: Blank control and 1,000 mg/L Element values: No acute inhibitory effect on activated sludge respiration rate at 1000 mg/L with a contact time of 6 minutes. Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the test substance remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS ____________________________________________ The test substance exhibited no inhibitory effects at 1000 mg/L over a contact time of 6 minutes. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ R eliability: Klimisch ranking 3. Testing lacks record of methodology used. The method was not an agency-approved method. Test duration was not long enough to definitively determine if there might be other adverse effects that might occur during typical wastewater treatment retention times. Sample purity was not characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_______________________________________________ This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request number 6812S, 1981. OTHER_____________________________________________________ Last changed: 6/26/00 I 001653 L-rttV: SLUDGE TOX. RESULTS 001654 9 8 7 6 5 k 3 2 1 O 3M Environm ental Laboratory Ac u t e T o x i c i t y of a Compound (Substance) tO i-A ctivated Sludge Mixed L i q u o r Protocol R eference: EAR 11/ 8/79 Lab request n o .: !X S D ate; '7 -0/-S/ A n a ly s t: J. Q. The a c t iv a t e d sludge mixed liq u o r was c o lle c t e d from the S t . P a u l/M in n e a p o lis Metro Wastewater P la n t on 7 - 0 /- C / Sample d e s c r ip t io n :. L. - ^_$ jj -. Z X - y J E & f c if L q X.~-<L(____ P re p a ra tio n of sto ck s o lu t io n : O>(o0 grains of sample were d ilu t e d to /QO w ith d e io n ize d reagent w ate r. Date "of1p re p a ra tio n :, ~ 7 _____ ml TIME min. sec. /r dhHr A$r:NTS ^ 'r < Wm g/l T MATER 1Al | 'B /l 1 K " GE y I S O 1 .9 3 7 -1 * 7 .to 6>.9 0 . v r X -- .X 3 - -- f .o r Y-- r-- . . y r -- /.US' 6 .5 ~ 7-- -- 2 *** 2 . / S' Y /.s' JO -- A X O // -- COMMENTS: BEST copy AVAILABLE 001656 TM USS` L^ ` M t fe tes ,n e u e e -n> -Htoouce K tu itiA * TM x l " tr4 BEST COPY AVAILABLE r'm m ****** ^ IM UIjH ! 001657