Document 2N2GrymV8ooBnLE2dpB8xvn7p

f' r-o.ce IT r. Lot;); >. o 1 m 1972 jj| General aiccuaoion i, ii r .nm; inn ni? pnq TP HUTAN BLOO'j) AND ADT>,r,SJ3 T'TSSn-; T` fii'rOM .III>PJS:T ^T* Rr&BAWP OROVPS BY J3J3VA KA*mNJ2N . M.Sc. . Jitate Veterinary Medical Tnatirnte Helsinki, Finland LA1JRI KOLHO M.D. Oy Nokia Ah Nokia, Finland r.4. **?*,*1S . 0511*96 j i . *. TOWOLDMON0059017 r ;if t r [f nij U ;r i'.jL l! 1 2 PEC1972 | mho orrf'ntT,tion of PCS in human blood and ndimee tlorue ir three ni-n'eront research groups ), Karpp^n^n, L. *<olho Tnri^v it has boon definitely proved that FCB can he found evor*y''hr? in our surroundi and in living orpanip'-n, wo fifin are learning constantly about its biological effects on different species. Knowledge about the harmful effects on human beings oaused by PCB has been obtained chiefly from old souroen or from ecoidents and we have very vague Ir'eno nbont the doses or tissue concentrations that hove any noticeable effect. Much confusion has also arisen because the crtr'ii'O^cial PCri-nroductR have differences that concern i'OB itsoi.f and especially impurities in then. In induatrinl toxicolo--r ft is often useful to know the tissue concentration of ci'r'!,il?t.Jve compounds and use it as an indox of eyo^ure which ot'ipr'viae may be very difficult to estimate. Therefore vro hove determined the concentration of FOB in blood and J" no^c cases also in subcutaneous adipos* tissue of 29 persons. JMne pennons so far as we know have never been professional!y exposed to VOB ( group I, persons 1 9 ). Bix other nor^ono handle ?0d sa*rr>!.ee in an analytical laboratory ( group II, persons 10 - 15 ) and eleven persons have been working since 15;6d at a capacitor factory where Arochlor 1242 in used for impregnation of capacitors ( group III, persons 16-27 ). The PCB concentration in the air of this factory has been proved to fulfil internationally accepted limits. Special attention has been given to the protection of the shin. The PCB concentration in blood and adipose tissue nas been determined by gr.a chromatographic method which in presented in dtnil in the appendix of Matti tfelminer'e lecture. In **1 urea 1 and 2 there are the results of the gas chromato grams of the blood and subcutaneous adipose tissue of some Ubll<t*7 TOWOLDMONOQ59018 *!'hp obsarvad TO'' core^i''\rat.i om h"vr been i r ('>: h-'i i >i tn,hi1 -- p and `he concentrations am > In f-'/lr'- ( nii'i ), On'TKm^n v/c am a.v/nre of the possible error involmd in lining onlv one method, Uni.r.'': only an electron capture det^ctm* thn identification and oiiantitative determination ef th different POM compounds is diffucult, The problem is even mom ooreilooted since the relative heights of POM peak? oh.; i'-''* in hi of n-rj'nl tissues ( Pig. 1 and ? ). 'fb* o'lVf vofit.o^rn'in fron some human blood and adimee evh-.-r.otn dr not reeemhle those of tne technical product?, li'.i'-f-'nrinrn i.t f obvious that several recks contain dU'h'rppt compounds dm to overiPr'ning peeks, Uolnr >xj d^i.i-ntcr, onnoentrntjone can only be estimated by ooMr.-riann to the rprnnnqn of s known amount exactly sere the cor'oHn'1. -io-rev^r, the relative differences in the concentrations of PCBs of most samples is very hi~b and the reliability of the moults is acceptable. All thp emr'lred y^emons are in p;ood health. However, only + i,r. j r> yro"p TIT have boon under special med.icrl ob<'.'Tvatior b"t in ap.ite of approximately 5 tims larger *'(,*c concentration in their blood compared with control 1yronn T we have been nnphl to detent any biological effect by POM in them. 0 51i<r9ti 1 1 TOWOLDMONOQ59019 h TOWOLDMON0059020 i fr 7~. --I i 1I Eza I[ E3 C3 CZ3 C3 r-! E3 d 1 CT3 C3 II!' :iiI i ESS 7 : r--, I *I ;. i TOWOLDMONOQ59021 era on Ore i. Persons with out any special exposure to PC J. No | Vr'et weig ot basis -5/ BBS PCB Fat ^ Fat basis ng/kg DDE i-CB 1 . <s Tampere 2. 0 n 3. Helsinki 4. <r " 5. 9 Tampere 6. 9 " 7. c/ " 8. 9 ' 9. 0 " " o oo 0,0046 0,0014 0,0016 0,0007 0,0008 0,0018 0,0005 0,0041 0,012 0,0095 0,0073 0,0061 0,0041 0,0049 0,011 0,0031 0,0056 0,13 0,14 0,14 0,16 0,07 0,13 0,15 0,08 0,15 3,6 9,9 0,77I . 6,6 1,1 5,4 i,i 3,9 0,91 0,60 5,5 3,7 1,2 7,0 0,55 2,7 3,8 3,6 1 1 I : Group 2. Persons handling PCB in an analytical laboratory.; 10. 9 Helsinki <0,005 11. 9 n <0,005 12. 9 n <0,005 13. 9 H. 9 " w <0,005 <0,005 15. 9 " <0,005 0,036 0,040 0,046 0,050 0,063 0,062 0,10 0,12 0,10 0,08 0,09 0,09 ^n.c. n.c. n.c. n.c. n.c. n.c. 35 33 46 61 71 70 2)5,5 5,5 2,5 1,0 <1 <1 1) _n.c. = not calculated 2) Years of reasearch work with PCB. \2 U5 1 1301 TOWOLDMONOQ59022 J2 0CC):'? Table 2. TVDB and FOB in human "blood. Group 3. Workers of a capacitor factory. n.c. = not calculated ubiisui TOWOLDMONOQ59023 U llL C I','/: % V i\ i i! I .I f Table 3. DDE, DDT and PC3 in human blood and subcutaneous adipose tissue. Wet weight Adipose tissue Fat basis No DDE PCS Pat 1-. DDE PC3 DDE DDT PCB 1. 0,005 0,012 0,13 2. cO, 005 0,010 0,14 3,6 0,77 9,9 2,7 1.5 6,6 0,81 0,35 2.3 1.5 28. <0,005 0,11 0,15 2.3 76 1,2 }n.d. 11 29. ^n.a. n.a. n.a - - 3,8 n.d. 200 16. 0,005 0,78 0,26 1 ,3 305 ^n.c. n.d. 285 (atheroma) o Co) U1 21. 0,34 0,09 4,2 400 3,4 n.d. 160 17. 0,008 1,9 0,27 3,2 700 2,9 n.d. 635 1) n.d. 2) n.a. 3) n.c. = not detected = not analysed = not calculated cj ca t--i sa 0511303 TOWOLDMONOQ59024