Document 2JwkQ92db3xeaQr9716xkXVRN

AR R26 - 0757 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE identity: A mixture containing perfiuorooctanesulfonate, which may also be referred to as PFOS, Octanesulfonic acid) FC-95, (CAS # or as a component 2795-39-3). of FC-203CE. (1- Remarks: The 3M production lot was not noted. The test sample is FC- 203CE. Current information indicates itis a mixture of 1.84% PFOS, 30% dsioedtihuymleoncetygllyscuollfabtuet,yl2.e1th%erp,o6ly0o.x2y3e%thwyalteenre, m2o.n5o6o%ctSyullpthoenneylfoeatmheerr,, 3.1% 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. Tchhaerafcotlelrowiiznegd scounmcmeantrryataipopnlsieosftiompaumriitxiteusr.eDwaittah imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical component of the test sample. METHOD: Method: OECD 201 and EPA-600/9-78-018 Type: Acute Static GLP: Year No study performed: 1991 Species: Source: Selenastrum capricomutum In-house culture. Originally from the Culture Collection of Algae at tEhleemUneinvterbsaistiyso:f TAelgxaalscaetllAcusotuinnt.s (cells/ml) Exposure period: 96 hours Statistical Methods: Performed using standard statistical techniques. ASntaelpyhtainc,al19m8o3n.itNorOiEngC:wapsH caanldcutleamtpeedrautsuirneg:Dunnett's test. Test Conditions: AdeligoanliNzuetdrwiaetnetr.MeTdhiiusmn:utrNiuetnrtimenetdmieudmipurmovpiedredU.aSl.l mEiPnAe,ral19n7u8trpiernetpsaersesdenitnial for algal growth and also served as the diluent for all algal operations. The pStHoocfktahinsdsytnetshtetsiocluatlgiaoln medium was ~8.0. preparation: A 1000 mg/L primary stock solution was prepared in algal medium. Aliquots were added directly to algal medium in test vessels to create test solutions. Esoxlputoisounraenvdecsaseplpse:d 250 with mL Erlenmeyer flasks containing inverted glass beakers. 100 mL test Agitation: Continuous rotary shaker, rate of rotation not given. Number of replicates: 3 Initial cell loading: 1.0 x 10 cells/mL. 001160 Numberof concentrations: five plus a blank control Lliamgphst.ing: Continuous lighting at 50 uEin/sec/m? using cool-white fluorescent Water pChHemriasntgrey(:0 - 96 hours): 8.29.9 (control exposure) Test te8m.p3er8a.t6ur(e1,r0a00ngmeg(/0L--ex9p6oshuoruer)s): 23.0 - 23.4C (incubator) RESULTS Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L Algal Growth Response at96 Hours Nominal Exposure | Avg.Cell-Count|Avg.Specific (cells/mL) Growth rate Conc. (mg 479000 | 0040 | -- [aso igsoo0 [250| 58000 | | 003 [22 oo0t8 | es | | 20,000 [0| 00 8 7| [eo| 3000 | 002 | Hoo | 100[ 0 o | 0.000 (7) Average growth rate in control compared to test concentration growth rate. Element values: 96-hour ECso = 230 (212 -- 248) mg/L 96-hour NOEC = <150 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not determined. Remarks: `Substance Testing was conducted on Remarks field. The values a mixture reported as described in the Test apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS Tcohnefitdesetncseubisnttearnvcaleoefxh2i1b2ittsoa29468-hmog/uLr.ECTshoeof962-3h0oumrg/NLo wOibtsh earv9e5d%Effect Concentration (NOEC) was <150 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 001161 patAQuALTY Reliability: Kiimisch ranking = 2. Testing meets the criteria for quality tJeasctkisnga.naHlyotwiecavlerc,onsfairmmpalteiopnuorfitythweaasmnooutnptroofpefruloyrcohcahreamcitcearlizperdopaonrdtitohneisnttuhdey solution. REFERENCES "This test was conducted by EnviroSystems Division of Resource Analysts, Incorporated, of Hampton, NH at the requestofthe 3M `Company, Lab Request number J1884-1, 1991. 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Growthdata from toxicity test i 9 - SRT T SsAeTE:ae TI [ER Mo-- y -- % Tudle A.1. Tasperature nessured during toxicity test 1 38 eble 2.2. FVaaigus : 38measured deci bid toxicityotest geie" LE Pata 5ds AE S Cdh nlo El eBl L HleGs E SR Eset oe By onli ooCaE nU aRl. B Fe REe CEDESEL -a bcetie RE RERE ESHA 5 EUR a ie ERNE Ll EE RT bi [ Le Ee E a: eR ee 4 bs ' PE 001166 . EZ ) wv. omarion evenitao staBtEyT Ie HuernhR E R postoreo L sCbay 0 Coven, Asar 5r 8lhue, TeHoaiy teetrsno,f T0hhae ! 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B SCOEREE e Sa Tw Rey Sn e o agSea al CooSn asa | E aaaa Baalis nl PGT icRR GHD le?i)5YL RCEENSR DAEL OMTB PIR ruaR DR IR SR Bn EL LEE RR | ; CIN TT Fl Fl ) } 3 001170 N nr 8 yr Rp ES BEST copPY AVAILABLE | 2 radia 2. + Growth datafrom toxicitetsty monSdeoeaeln | reae ber ofwe Cellsrpc aer_Mie li7tmxa1r0 (host) n ee 1B OB Room 8 E iE sooE s EB 8 8 B mouw B : E.: L2ioosr10 Low 1oo0x 8& oma B Boxe10 8% {aE me S AR CaEriE na wI e RRN min cS e a3P % ohneTish aa iHib sea ua ahEo E iGn n n EA E T make gein Tanne LxR B0lS %aSnanek 3a hea SEhAea n GS B io npE Ebaea E RA ae L C en a ok PL A ay dks eae a d Ia a Bcen aad AE Eeoe Sac he | ia SEis Be IN ] The 3. Dporcras cahangeytron control aad average spcitic gronth rate --_co--n_ot--mtimstion of fort mhatines CTerrio oertarceittm is fFoercocsecntcocanhetel B -- aria TT or eneT me oerr Meeeraaem aTrEsavrn 00 cote) 0.0 0.052 0.0 000 oo 150 com ow ow oom 6 Bm mm 50 ou 00% oom oui sw wu ss wo Ps oon oon ous oor 6 wm 6 ow om oo someon is 3 6 0 _ Lovo oon 0s oom 000 as so 6 10 _-- BEST COPY AVAILABLE EE ES M La pTh ia gs aCavg I. B oLBm o lro Eoa sibat eteeoiaene3n D i ca uee le ee RSE(rEgaEeeRgTSnaIdeAyd L PeE a a s e Jade O aR e SEER e Lo e Tmae a s]3 FERE S Ee sO a MTE ae Ee e e Cr hEsSEea BE SOa E Lo iasnDSENT aRee arie a " Ps ) " ` 001172 I" i Table 4. Mattes attoctive concentrations (8CS0n) fro toxicity tant Tm i Eperiod e e BwsR0 coe e ntd Ttts e e waeiidd ners 1,000 si - - Gren casomn - - Tews Msn 20- Gta meriss erase shes Bosna wn rat BEST Copy ffar pi LEE aE SlEgR es ' SE gShal LSlHEE GEhEeR e 3 Soka MfEe i+ SRERC Lo aan pn B a EaST E dil BghooPa nR R l R S ok Bai Rein> ana Br m SERGy E Ce 0 oe aE RO REE ; j rE R LaighS ins Es a Scoa (= 3 oI bi 22 " -) . i) 001173 FRLETTEAEN EL Henant crane EA 1. arn GSiEoaDhn.ic r1a3v0en.s.GultTaaltioaaed f20o1r,TFaisctei,ng.coofovCihhemTieciablisi.tonfaTcetriro,n 2:`dRoepteedctsSunoen SPtearpmheannl,l cCiEi.att1i9t83i.onCoster progr for caleslation of 1450 values. 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