Document 2JrV9X8GG6wp6QDvexx71z5MN

ASSESSMENT OF THE PERSISTENCE OF POLYCHLORINATED BIPHENYLS IN BIOLOGICAL SYSTEMS ID L I11 PLAINTIFF'S EXHIBIT . NEV 0Z6032 ,TffL'J A W . J A * .V ..III. ILM ! ^ " H 739287 r 739288 % COMPOSITION OF POLYCHLORINATED BIPHENYL PRODUCTS* HOMOLOG # Cl/BIPHENYL 0 1 z 3 4 5+ 4 AROCLOR 1221 10.8 51.0 32.0 4.2 1.6 0.3 AROCLOR 1016 1.0 19.4 64.5 15.0 0.2 *PER CENT (tf/W) BT GC/MASS AROCLOR 1242 0.7 IS.6 54.5 22.5 6.7 AROCLOR 1254 0.2 0.6 23.0 77.2 *i otOoUUv'JJ n V ' JfL, *& Electron Capture Gas Chromatograms Aroclor 1221 JL jWM*_ Aroclor 1242 739289 739290 jfC Electron Capture 60s Chromofogram Low Resolution - 61 Packed Column J__________ I___________I________:__ I__________ I 2 4 6 8 10 Flame Ionization 60s Chromatogram I High Resolution - I O O ' Support Conted Open Tubular Column J 1 _L_ 20 25 30 / PRIMARY BACTERIAL DEGRADATION STUDIES * SEMI-CONTINUOUS ACTIVATED SLUDGE DEGRADATION RESIDUE ACCUMULATION STUDIES . FISH - CATFISH AND BLUEGILLS . BIRDS - WHITE LEGHORN CHICKENS MAMMALS - ALBINO RATS AND BEAGLE DOGS mmmm NEV 026036 m 739291 AROCLOR SEMI-CONTINUOUS ACTIVATED SLUDGE PRIMARY DEGRADATION STUDIES NEV 026037 739292 i*. * . Semi-Continuous Activited Sludge Test Unit NV 026038 739293 MECHANICAL CYCLE SYNTHETIC'SEWAGE AND AROCLOR (Img) ADDED * NEV 026039 4 739294 Semi-Continuous Activated Sludge Degradation of Polychlorinated Biphenyls Mean % Degradation Rate (48hr Cycle) `Degree of Chlorination /Q NE V 26iH 739295 Semi-Continuous Activitated Sludge Degradation of Aroclor 1242 Elution Time (minutes) NEV 0260^1 739296 . BIPHENYL, MONO-, DI-, TRI-, AND TETRACHLORO BIPHENYL HOMOLOGS CAN UNDERGO PRIMARY BACTERIAL DEGRADATION . RATE OF PRIMARY DEGRADATION DECREASES AS THE DEGREE OF CHLORINATION OF THE AROCLOR PRODUCT IS INCREASED BIPHENYL>AROCLOR 1221>>AROCLOR 1016>AR0CL0R 1242>AR0CL0R 1254 NEV 026042 739297 .AROCLOR RESIDUE STUDIES NEV 026043 739298 RESIDUE STUDIES* - CATFISH AND BLUEGILL FINGERLINGS .21 DAY DYNAMIC PRODUCTS STUDIED NUMBER OF FISH NUMBER OF SAMPLES WHOLE FISH NUMBER OF SAMPLES ANALYZED WHOLE FISH AROCLOR 1016 AROCLOR 1242 270 " 24 BIONOMICS, INC. WAREHAM, MASSACHUSETTS NEY 02604% 739299 RESIDUE STUDIES*- BEAGLE DOGS I/'li S , PRODUCTS STUDIED 2 YEAR * CHRONIC ORAL AROCLOR 1242 AROCLOR 1254 .AROCLOR .1260 90 DAY SUBACUTE ORAL . AROCLOR NUMBER ) FEMALE OF DOGS) MALE NUMBER } MUSCLE OF ) LIVER SAMPLES) FAT 40 40 54 54 : 54 14 14 7 7 :7 TOTAL SAMPLES COLLECTED 242 21 NUMBER ) HUSCLe SAMPLES) LIVER ANALYZED) ''M, 42 40 43 7 7 7 TOTAL ANALYZED 125 21 INDUSTRIAL BIO-TEST LABORATORIES, INC. NORTHBROOK, ILLINOIS F G c-i> / JOj too . NV 026045 739300 RESIDUE STUDIES* - ALBINO RATS PRODUCTS STUDIED NUMBER] FEMALE OF RATS] MALE NUMBERj 'OF SAMPLES] MUSCLE KIDNEY LIVER FAT PUPS NUMBER] OF samples; ANALYZED] MUSCLE KIDNEY LIVER FAT PUPS 30 DAY ORAL AROCLOR 1242 AROCLOR 1254 AROCLOR 1260 115 115 18 18 18 18 - 18 18 18 18 2 YEAR CHRONIC ORAL AROclOR 1242 AROCLOR 1254 AROCLOR 1260 500 500 33 - 33 33 - 33 33 33 3 GENERATION RAT REPRODUCTION AROCLOR 1242 AROCLOR 1254 AROCLOR 1260 ^20 ~20 - 10 - 10 KM 90 DAY SUBACUTE ORAL AROCLOR 1221 60 60 7 - 7 7 - 7 7 7 - TOTAL SAMPLES COLLECTED 144 198 TOTAL ANALYZED 72 99 pE2> L e v t L i : \f 10-0 pp ' INDUSTRIAL BIO-TEST LABORATORIES, INC. NORTHBROOK, ILLINOIS 20 10 42 21 NEV 0^6046 4 739301 RESIDUE STUDIES* - WHITE LEGHORN CHICKENS 9 PRODUCTS STUDIED . .. NUMBER ) FEMALE CHIC0KFENS) MALE MUSCLE LIVER FAT EGGS CHICKS CHICKENS REPRODUCTION RESIDUE AROCLOR 1242 AROCLOR 1254 AROCLOR 1260 . AROCLOR 1242 200 80 40 16 40 40 . 40 ^250 30 8 8 8 M>0 10 NUMBER) OF ) SAMPLES) ANALYZED) MUSCLE LIVER FAT EGGS CHICKS 14 5 12 4 18 5 25 8 11 : 10 ,ES COLLECTED 'ZED 400 80 121 32 INDUSTRIAL BIO-TEST LABORATORIES, INC. NORTHBROOK, ILLINOIS NEV 020 O'*7 739302 TWO YEAR CHRONIC ORAL EXPOSURE RESIDUE. STUDY OF AROCLOR 1242, AROCLOR 1254, AND AROCLOR 1260 IN BEADLE DOGS NEV 0260^8 739303 739304 RESIDUE STUDY OF AROCLOR 1242 IN BEAGLE DOGS TWO YEAR CHRONIC ORAL EXPOSURE ORAL EXPOSURE LEVEL PPM PCBs 1 10 100 RESIDUE, IF TOTAL 519 5186 51865 FED RETAINED RESIDUE, 2 YEAR EXPOSURE 2 6 12 RESIDUE, 30 DAY RECOVERY 1 4 10 RESIDUE, 60 DAY 0.8 3 6 RECOVERY , HOMOLOG DISTRIBUTION 1 2 3 4 5 6 7 8 9 10 - (2) (3) (4) (5) 6 - - (3) (4) 5 6 (7) (8) . - - - 5 (6) (7) (8) - - - 5 6 (7) (8) - - \ .v - AVERAGE PPM IN LIPID - ALL TISSUES ( )ELECTRON CAPTURE PEAKS GREATER THAN 5Z OF TOTAL 6*70970 A3N P y NEV 02oU50 RESIDUE STUDY OF AROCLOR 1254 IN BEAGLE DOGS THO YEAR CHRONIC ORAL EXPOSURE ORAL EXPOSURE LEVEL PPM PCBs 1 10 100 HOMOLOG DISTRIBUTION 1 2 3 4 5 . 6 7 8 9 10 RESIDUE, IF TOTAL 519 5186 51865 FED RETAINED - 3 (4) (5) (6) 7 - - * RESIDUE, 2 YEAR EXPOSURE RESIDUE, 30 DAY RECOVERY RESIDUE, 60 DAY RECOVERY 7 30 132 5 24 109 4 20 88 - - - (5) (6) (7) 8 - - - - 5 (6) (7) 8 - j - - 5 (6) (7) (8) m - *AVERAGE PPM IN LIPID - ALL TISSUES -Nl CO CD ( )ELECTRON CAPTURE FEARS GREATER THAN 51 OP TOTAL 9 RESIDUE STUDY OF AROCLOR 1260 IN BEAGLE DOGS TMO YEAR CHRONIC ORAL EXPOSURE HOMOLOG DISTRIBUTION PPM PCBs . 1 2 3 4 5 6 7 8 9 10 ORAL EXPOSURE LEVEL 1 10 100 ` RESIDUE, IF TOTAL 519 5186 51865 FED RETAINED - - - (5) (6) (7) 8 _ RESIDUE, 2 YEAR EXPOSURE 8 63 388 - (6) 7 (8) - - RESIDUE, 30 DAY RECOVERY 7 60 363 - - " - (6) (7) (8) - - RESIDUE, 60 DAY RECOVERY 7 55 337 - 6 (7) (8) - - AVERAGE PPM IN LIPID - ALL TISSUES ( )ELECTRON CAPTURE PEARS GREATER THAN 51 OP TOTAL NEV 02b051 GENERAL CONCLUSIONS RESIDUE BUILDUP RATE . INCREASED. AS THE EXPOSURE LEVEL INCREASED . INCREASED AS THE EXPOSURE PERIOD INCREASED . DECREASED EXPONENTIALLY AS THE DEGREE OF CHLORINATION OF THE PRODUCT FED DECREASED . AROCLOR 1260>AR0CL0R 1254AR0CL0R 1242 >AROCLOR 1221 . FISHCHICKENS>RATS>DOGS . CHICKENS (LIPID): FAT = MUSCLE>LIVER RATS (LIPID): LIVER>FAT = MUSCLE>KIDNEY DOGS (LIPID): LIVER>FAT * MUSCLE NV 026052 739307 Average ppm PCB in Lipid (Muscle, Fat,Liver) GENERAL CONCLUSIONS RESIDUE BUILOUP RATE . INCREASED. AS THE EXPOSURE LEVEL INCREASED . INCREASED AS THE EXPOSURE PERIOD INCREASED . DECREASED EXPONENTIALLY AS THE DEGREE OF CHLORINATION OF THE PRODUCT FED DECREASED . AROCLOR 1260>AROCLOR 1254AR0CL0R 1242 >AROCLOR 1221 . FISH>>>>CHICKENS>RATS>DOGS . CHICKENS (LIPID): FAT = MUSCLE>LIVER RATS (LIPID): LIVER>FAT = HUSCLE>KIDNEY DOGS (LIPID): LIVER>FAT = MUSCLE NV 026Qb*t 739309 BLUEGILLS CATFISH WHITE LEGHORN CHICKENS ALBINO RATS BEAGLE DOGS EXPOSURE EXPOSURE LEVEL (PPM) PERIOD CONCENTRATION . . - FACTOR* 0.1 (MATER) 0.1 (WATER) 21 DAYS 21 DAYS 400 v000 320,000 1 .0 (FEED) 12 MEEKS 20 1.0 (FEED) 1.0 (FEED) 2 YEARS 2 YEARS 9 2 CONCENTRATION FACTORS DO NOT TAKE INTO ACCOUNT EXPOSURE PERIODS NEV 026U5 739310 .GENERAL CONCLUSIONS . RESIDUE BUILDUP RATE . INCREASED,AS THE EXPOSURE LEVEL INCREASED . INCREASED AS THE EXPOSURE PERIOD INCREASED . DECREASED EXPONENTIALLY AS THE DEGREE OF CHLORINATION OF THE PRODUCT FED DECREASED . AROCLOR 1260>AROCLOR 1254AROCLOR 1242 >AROCLOR 1221 . FI S H CHICKENS RATS>DOGS . CHICKENS (LIPID): FAT = MUSCLE>LIVER RATS (LIPID): LIVER>FAT = MUSCLE>KIDNEY DOGS (LIPID): LIVER>FAT *= MUSCLE NE y QbQ5b 739311 GENERAL CONCLUSIONS RESIDUE FALL OFF RATE . * INCREASED AS THE EXPOSURE LEVEL DECREASED . INCREASED AS THE DEGREE OF CHLORINATION OF THE PRODUCT FED DECREASED . AROCLOR 1242>AR0CL0R 1254>AR0CL0R 1260 . INCREASED AS RECOVERY PERIOD INCREASED . DOGS>CHICKENS NEV 026057 739312 y/Wict s* yjrtoto / Si/j/wt# -?3/T9-p " ' e y / 3 { J.t* J'SOp' Z 7 ? t / & & /*/ V 3 t S -O e t> ~>lr& Q IfM Q t Q & Y & /K , Q^> / GENERAL CONCLUSIONS RESIDUE FALL OFF RATE . * . INCREASED AS THE EXPOSURE LEVEL DECREASED . INCREASED AS THE DEGREE OF CHLORINATION OF THE PRODUCT FED DECREASED . AROCLOR 1242>AR0CL0R 1254>AROCLOR 1260 . INCREASED AS RECOVERY PERIOD INCREASED . DOGS>CHICKENS NEV Q2b059 739314 GENERAL CONCLUSIONS III. ALTERATION OF THE HOMOLOG DISTRIBUTION OF PRODUCTS FED' . INDEPENDENT OF EXPOSURE LEVEL . INDEPENDENT OF EXPOSURE PERIOD . HIGHER CHLORINATED HOMOLOGS DOMINANTLY RETAINED (PENTA-, HEXA-, HEPTA-, OCTACHLORO BIPHENYLS) . DOGS>RATS>CHICKENS>FISH* . INCREASED AS DEGREE OF CHLORINATION OF PRODUCT FED DECREASED AROCLOR 1 221>AR0CL0R 1242>AR0CL0R 1254 >AROCLOR 1260 DISTRIBUTION UNALTERED BY FISH NEV 0 2 6 0 6 0 739315 T90970 A3N RESIDUE STUDY OF AROCLOR 1242 IN UHITE LEGHORN CHICKENS ORAL EXPOSURE LEVEL PPM PCBs* 1 10 -100 1.2 HOMOLOG DISTRIBUTION . 3 . 4 5 6 7 8 .9 10 RESIDUE, IF TOTAL 126 1260 1 2 6 0 2 ( - (2) (3) (4) (5) 6 - - FED RETAINED - RESIDUE, 12 WEEK EXPOSURE .- RESIDUE, 30 DAY RECOVERY 14 136 1312 - (3) (4) (5) 6 - - 9 77 749 . - - (3) (4) (5) (6) - - AVERAGE PPM IN LIPID - ALL TISSUES ( )ELECTRON CAPTURE PEAKS GREATER THAN 5Z OF TiOTAL *. I/r - t ; 739316 RESIDUE STUDY OF AROCLOR 1Z54 WHITE LEGHORN .CHICKENS 2*50920 f ORAL EXPOSURE LEVEL PPM PCBs* 1 10 tOO ......... HOMOLOG DISTRIBUTION -- ..1 ...2 . . 3 .A ..5 .. 6 . 7 8 . 9 - 10 RESIDUE, IF TOTAL . 126 1260 12602 FED RETAINED 3 (4) (5) (6) 7 - RESIDUE, 12 WEEK '38 362 3506 EXPOSURE - 4 (5) (6) 7 ` - RESIDUE, 30 DAY 17 164 1580 RECOVERY ` 5 (6) 7 -, - AVERAGE.PPM IN LIPID - ALL TISSUES .( )ELECTRON CAPTURE PEAKS GREATER THAN 5X OF TOTAL .. /I I/F CO CO CO