Document 2JOozELz3BGydxnkNQR432v4g
ARRAG- 1262
Executive Summary - Biodegradation Screening Studies of 8-2 Telomer B Alcohol -
"ETMheStEwRo s1t5u-d0i3e/s4d8e4s2c)rirbeepdreisnentthethaettsaccrheeednirenpgoprhtsas(eEfMorStEhRe b1i3o-d0e3g/r4ad8a4b2ilaintdy assessment of 8-2 TelomeBr Alcohol (CAS# 678-39-7). The unique analytical challenges presented by
tthhiosrcohuegmhiustnrgye(alnodw iwnatgerofsohleubimleitry,s<voalattiolnithy,iapnodfsPtrhoynsg la-dscohrputmimoneatorsuarfpacee)sresqaulirseeat
`pqauraanmteittaetrisvetlhyatidmeantyifayfpfoetctentthiealabtirlaintsyftoormaacthiioenveprmoadsusctbsaliannccoemapnldextombaotrtihceqsu.aliTthateivperliymaanrdy tohbejelcotnigveeor-fttehrem bsicordeeegnriandgabsitluiditeysswtuadsietso, iwdheinctihfywitlhlesheavceritaincaolbpjaerctaimveetoefrdsepfriinoirtitvoellayunching identifying the ultimate transformation products formed by biotransformation of8-2 Telomer
B Alcohol.
dFeosritghneesdcarseefnoilnlgowpsh:ase, two cold (non-radiolabeled) studies were conducted in sequence and
= Achicwe the desed est condions 1. Ready Biodegradation (Modified OECD 301D Closed Bottle Test) to:
= With unacclimated inoculum from a municipal POTW
* With test chemicalasthe only intentional carbon source Test the hypothesis that ready biodegradation, ultimate biodegradation, and
Gdicsdut 1opental abiote removal mechani (5. vlality 42d primary biodegradation would be unlikely under these test conditions.
Confirm the expectation that mass balance will be difficult to achieve in cold
adsorption).
screning phase 2. AccelerAactheideBvieotdheegtreasdtactoinodintTioensst wtio:th the objectiveofoptimizing conditions for
`pote=ntiaWlibtihotprraen-safcocrlmiamtaitoensd.inoculum from an industrial wastewater treatment plant.
= With yeast extract added as additional carbon source to enable potential for co-metabolism.
Determine if any potential transformation products are formed in the
* iTdoenatlilfiocwatdieonvoeflpooptmeennttioalfstaramnpslfionrgmaatniodnapnarloydtuicctasl. mTeothpordespafroer
authentic standards based on the structural informationofthe
aidnedntqiufainetdiftircaantsifoonr.maTthieosnepraoreduncetcsestsoafraycisltietaptsepdreifoirnittoicveonidduecnttiifnigcation longer-term, more definitive biodegradability studies.
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CONTAIN NO CBU 0
cRoel-dcosntfudiiremstdhueeetxopepcottaetntiioanltahbaitomtiacsrsebmaolvaanlcemewiclhlabneidsimffsic(cu.lgt.,tovoalcathiileivtey ainnd adsorption).
ReadyBiodegradation (ModifiedOECD301DClosedBottleTest)Results: 1. cPoanrceennttCraotmiponosuonfd8-M2onTiteolrionmge:BrInAlbcoothhotlhestteeadtilsyysdteecmreaansdeda.biAotti2c8codnatyrso,ltvheessels, the acboinocteinctrcaotnitornoslovfess8e-l2s.TeTlhoimserinBdicAaltceoshtohlatwaebrieotnioctrdeimsotvinagluimsehcahblaenibsemtswe-en tests and avoclhaiteivleizmataisosn abanlda/nocrea.dsorption are likely to be active and must be accounted for to 2. F(lu1o7ri9d+e2A.n6aplysgias:t/dTahyLe0r;e w1e7.r1e+ no sipg0 gni/fLiaca8 tntdacyha7n;ge1s7i.n4fl1u.o4rpidegcoa/ntcdeLantyra1t4i:onand b1a7s.i5s,:o1n.e0muogl/eLoaft d8a-2y T2e8)loomveerr BtheAl2c8ohdoaly cteosntvpeerrtieodd.toCoonnesimdoelreinogftPhaFtOoAn waomuolldar tyiheeltdesttwiondmioclaetseodfthfaltuoPrFidOeA, tihsenaobtsleinkceeloy fa maanjionrcbrieoatsredanfslfuoorrmiadteicoonncpernotdruacttioofn8d-u2ring cToenlfiormmaetBrioAnlicsonheoelduenddearndthqeuaRnetaifdiycaBtiioodneogfraPdFabOiAlitpyretsesetnccoenodritaiobnsse.nceHowwilelveber, accomplished with a definitive study.
AcceleratedBiodegradationTestResults:
1. Parea.ntCInomthpeoaubniodtiMconcionttorroilsn,g:the concentrationsof8-2 Telomer B Alcohol steadily. (deMcordeiafsieedd aOtEraCteDs 3si0m1iDlarCtloostheodseBootbtsee)rvTeesdt.inTthhiesRienaddiycatBeisodtehgartaadbaiboitliicty troemaocvhaielvmeemcahsasnibsalmasn-cev.olatilization andlor adsorption must be accounted for
b.. By comparison to the abiotic controls,thetest system showed rapid primary b7io0t%raantsfDoarmyat1i4onanwditnhealrolsyo1f0p0ar%eantt dcahyem2i8c.alT-he3-l2osTsewlaosmcerleBaArlalcosyhoalreastult ofbiotransformation and not attributable from the abiotic loss alone.
2. FbliuootrriadnesfAonramleydsitso:flRueosruilntasteidndaicciadtseatnhadto8t-h2erTuenliodmeentrifBieAdlctorhaonslfworamsatriaopnidplryoducts as indicated by: a. Significant changes in fluoride concentration (10 g/L at day 0; 64 pg/L at day 14: and 70 pg/L at day 28) over the 28-day test period, a net increase of 60 pg/L due to 8-2 Telomer B alcohol defluorination.
3. Identificationof Transformation Products: Four fluorinated acid transformation products were monitored for and were identified in the test system by LC/MS/MS analysis and comparison with known authentic standards:
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i. F(CF2)/CF=CHCOOH (CAS# 161094764) - most abundant - 210 of
i.
total mass present F(CF2)sCH:COOH
(CASH
27854-31-5)
-
approx.
2%oftotal
mass
iii. pFr(eCseFn2t)yCOOH (PFOA, CAS# 335-67-1) - <2%oftotal mass present
iv. F(CF:);COOH (PHA, CAS# 307-24-4) - approx. 0.4%oftotal mass
pres>entItis hypothesized that PFOA may not bea stable
thraavnesfloeramdattoiofnorpmraotdiuocntoafnPdFthHaAt ddeufrliunorgitnhaetitoesnt.ofPFOA may.
v.. Owtehreernpoottednettiearlmtirnaends.formation products that formed during the test
`StudyConclusionsSummary
1. TtohePsFeOsAtuadnydr/eosrulottshesrugtgreasntsftohramtastuibosntapnrtoiadlucttrsanaspfpoeramrasttioonboefle8s-s2lTiekelloymuenrdBerA"lRceoahdoylBclieoadrelgyraidndaibcialtietdy"thcaotnaditlioenast. prHiomwaervyebri,odtehgerAacdcaetlieornaotfed8-B2ioTdeelgormaedartBioAnlsctouhdoilesis highly probable under favorable test conditions.
2. Tasheanpoitdeennttiiafliatbrlaentsrfaonrsmfaotrimoantpiroondpurcotdsucotf.8-H2oTweelvoerm,erthBisAsltcuodhyoilnmdiacyatiendctlhuadtePPFFOOAA mmaayy npoottebnteiaalmlyajuonrdebrigotorfaunrstfhoerrmadtefilounoprrinoadtuicotnatnodPtFhHerAe.arTehiinsdhicyaptoitohnessithsawtiPllFbOe:A explored in the forthcoming long-term biodegradability studies.
3.
Mass balance was allofthe potential
not a primary objective relevant transformation
ipnrotdheuscetssthuadvieesbaenedn
widaesntnioftieadchinietvheeds.e
Not
sCtAudSie#s.16O1n0l9y4-f7ou6r-4s;peFc(ifCiFc2p)oyteCnHti,aClOtOrHa,nsCfoArSma#ti2o7n85p4r-o3d1uc-t5s; [FF((CCFF22,)C,OCOFH=,CHCCAOSOHH,
335.67-1; identified
ianntdheF(aCccFe2l)e;raCtOeOdHs,tuCdyA.S#
307-24-4]
were
monitored
for
and
were
4. Abisodaengtriacidpaabtieldi,tytostaucdhiieesvweimllasnseebdaltaonacec,cotuhnettefsotrdaebisoitgincfroermloovnagl-tmeercmhanisms -- Volatility and adsorption. Therefore,theuse ofa '*C labeled study material is necessary to achieve mass balance.
5. TIhtes breesluiletvsoedftthhaetsleonsgc-reteenrimnbgisotduedgireasdasbhioluiltdy bsetuvdiieewse(daaersobiincdiacnadtiavnea,enrootbicco)nculsuisnivgea.
C labeled identify the
ssttuabdlye,mualtteirmiaatleatnrdanwsiftohrmaatdiuornatipornodoufct1s2.-18
months
will
beneededto
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