Document 2JDz8NYBOB2BZDdJYnGVOjkD5

FOR DU PONT USE ONLY AR226-2938 Du Pont HLR 55-89 Study Title StatljcAcute96^Hou^C50*of to Fathead! Mi nnowsTnrnephales promelas) Author. David G. Hutton Study Completed On February 20, 1989 Performing Laboratory E. I. du Pont de Nemours and Company, Inc. Haskell Laboratory for Toxicology and Industrial Medicine Elktjon Road, P. 0. Box 50 Newark, Delaware 19714 Laboratory Project ID Haskell Laboratory Report No. 55-89 Page 1 of 9 ''ompany Sanitized. Does noi contain T SC A C M I ; i qOOD LAfeORATORY PRACTICE STATEMENT Du Pont HLR 55-89 This study was conducted!according to EPA Good Laboratory Practice Regulations (40 CFR 792). Anjr areas of noncompliance are documented In the study records No deviations!existed that significantly affected the validity of the study. 1 Submlttei*: E. I. du Pont de Nemours and Company, Inc. Sponsor!: Chemicals and Pigments Department : E. I. du Pont de Nemours and Company, Inc. Study Director!: f( i v y David G. Hutton Consultant Environmental Sciences / f 7*9 - 2Coparj Sanuized. Does not contain TSCA CBI GENERAL INFORMATION Du Pont HLR 55-89 Material Tested; Medical Research No.: Haskell No.: Purity: Synonyms: 17,503 Submitter's Notebook Number: Sponsor: Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Wilmington, Delaware Material Submitted B y : Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Jackson Laboratory Study Initiated - Completed: Test initiated: 1/10/89 Test completed: 1/14/89 Study completed: 1/26/89 Notebook s : There are 9 pages in this report. Distribution: - 3ompany Sanitized. Does not contain TSCA CB! T Du Pont HLR 55-89 Title of Report Stit1^ut^6-HourLC50*of t ^ a H T a ^ f f m o w ^ M S e p n n e s promelas) Summary (H-17,503) exhibited no acute toxicity to fathead minnows, a warm freshwater fish, under ^ a t l c unaerated test ,, conditions during a 96-hour exposure period. The 96-hour L C 5 0 w a s >1000 mg/i.g bMiK"TfiF"wiiT'no'l i ^ T I ^ at n cm ln ^ T te s^ o n ce n tra ^ n ^ ^ ^ n d iSiES/ S& io" The solubility of u " l . as there was und...ed material in all test concentrations. Reported by: James ;W. Wetzel Technicien ./ . . Mo/Day/Yr Study Director: David 'G. Hutton Consultant Envir0nmental Sciences Mo/Day/Yr Approved by: -?A Lx\:, Frank X. Phillips,jPh.DT _ //,? " Mo/Day/Yr Section Supervisor ._ . Environmental Toxicology and Information Services Reviewed and Approved for Issue: Jj. David^G. Hutton Study Di rector (J sL*il?. Mo/Day/Yr JWW/DGH/FXP/smk/AQRP23.4 -4Company Sanitized. Does not contain TSCA CBI I Du Pont HLR 55-89 QUALITY ASSURANCE DOCUMENTATION STUDY: fl# 17,503 Static Acute 96-Hour LC50 of to Fathead Minnows (Pimephales promelas) AUDITS: Items Audited Protocol Evaluation Audit Dates 1/6/89 SHORT-TERM AUDIT REPORT NUMBER,: DATE FINDINGS REPORTED TO MANAGEMENT AND STUDY DIRECTOR: 1/6/89 Reported by: William J. Lynam Quality Assurance Auditor Date ,........... . t - n<?any Sanitized. Does not contain TSCA CBt BSS99 DU Pont HLR 55-89 Introductlon/Purpose To determine the static acute;toxicity o f L -- --- (H-17,503) to fathead m1jino*s, a warm freshwater fish. Materials/Methnds (Aquatic SQ^-TOZ) prepared as a 1.0 wa^ e r 'iolH Sf!ced In" 5-liter aquaria. And diluted with laboratory we 1 water to yield the desired nominal exposuri concentrations In 4 liter final volumes (12-cm liquid depth). Two Identical; vessels containing only laboratory well water were designated as the H^O controls. . ......... Chemical stability of the test material in well water was not determined. Ten juvenile, unsexed fathead; minnows (Plmephales promelaj) ^ a!k?JJ"Jred stock with a 1.6 cm mean standard length {range 1.2 to 1.8 cm) a n d ?'a 9 mean wet weight (range 0.015 to 0.078 g) were randomly placed ,n the test vessels two test vessels peri concentration. Loading was 0.12 g f1sh/L. Fathead'minnows are Identified by labeling the c u l t u r e r f t h ^ fish were maintained at Haskell 1 Laboratory for approximately 163 days, with the last 110 days in a 272-liter Ffberglas tank (84-cm in diameter by about 49-aii liquid depth) before being used for thi* fce*t ` .H0ldi"?o5anPThetUr6 within 3 "C of testing temperature throughout the holding period. The dissolved oxygen concentratidn during acclimatization ^ assumed to be at or cat iirat ion because air was continuously bubbled through tne water. no f r w t m f n r f f the H s S ?or diseases was required during the ho ding period, . F i s h ^ e r e Sot fed for approximately 72 hours prior to nor during the exposure. The test solution? were not aerated, and J** 16 maintained between 21.7C and 22.6C. Photoperiod was maintained at 16 hours 11 gh t :8 hours dark. Mortality counts and observations were mad approximately every 24 hours during the 96-hour exposure period. Dissolved oxygen and pH were measured in the H20 control and TM the low, medium and high test concentrations at the beginning of the >.est a,.d at aDoroximatelv 24-hour intervals during the 96-hour exposure period. Total alkalinity, hardness (EDTA), and conductivity were measured at the beginning of the test 1n the H-0 control. . Records All records from this test and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine Delaware) or archived at the Du Pont Records Management Center imiming ..on, Deiawa. e,. -6 Company Sanitized. Does no contain TSC Ct?r Du Pont HLR 55-89 Results J a ^ r e s u l t s for fathead mlhnows exposed to| __ _ | P ( H - 1 7 , 5 0 3 ) are presented 1n Table 1. Yne so-nour LC50 was >1000 mg/ liecause there were no mortalities at nominal test concentrations up to and Including 1000 mg/L. All test solutions (from 360 to 1000 mg/L) were milky with undissolved test material floating on the Surface and settling to the bottom. The chemical and physical parameters measured during the test of ^re reported In Table 2. All were within acceptable ranges for this tudy. Dlscussion/Conclusion ______ has been tested for acute toxicity with fathead minnows, a warm freshwater fish. Under the conditions of this test, was found to exhibit no acute toxicity at test concentrations abov^ Its solubility limit in water. A preliminary rangeffnding test on this compound was run but data are not reported here. In this test all the fish survived at dose levels of 1000, 100, 10, 1, and 0.1 mg/L, plus a HgO control. Footnote aLC50 = That concentration which 1s lethal to 50< of a test population during the specified time period. 7 Company Sanitized. Does not contain TSC A CBi RESULTS OF A 96 FATHEAD MINNOWS EXPOSED TO Ou Pont HLft 5S-89 TOXICITY TEST WITH fM-- 17.503) Nominal Test Concentrations . imq/L)___ H2O Control 360 600 1000 Cumulative Observed Mortality (%) .24 -Hr.- 48 Hr. 72 Hrr 96 Hr. A* A* ~ W ~ B* 00 00 00 00 00 00 00 00 d0 00 00 00 00 00 00 00 `Replicate exposure chambers containing ten fish in each. - 3Company Sanitized. Does not contain T SC A Cfll I Du Pont HLR 55-89 TA8LE 2 RESULTS OF PHYSICAL AND CHEMICAL PARAMETERS cATutrtn MEA5URED W R I N G A 196-HOUR STATIC ACUTE TOXICITY FATHEAD MIIIIIIIII |l||||||| |i|^-- -- -- -- TH JH- P W I Physical/Chemical Parameters Dissolved Oxygen (mg/L} Norolfoal Test Concentrations fmq/L) ~po ' W ko~ -- (Hjfgh) (Medium) (Low) 0 Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. 8>6 8.7 8.7 7>6 7.7 7.7 6.8 7.1 7.3 6^ 5 6.9 7.1 6*9 7.1 7.5 h9o Control 8.5 8.0 7.7 7.5 7.8 M O Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. 7*6 7.6 7.6 7.6 7*1 7.1 7.1 7.5 7*4 7.4 7.4 7.7 7*3 7.3 7.3 7.6 7*4 7.4 7.5 7.6 Water Chemistry on the H0 Control at 0 Hours Total Alkalinity (mg/L as CaCQj) 90 EDTA Hardness (mg/L as CaCO-j) 70 Conductivity Qiinhos/cm) 150 .. . ' "mpany Sanitized. Does not contain T SC A CBS