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AR 286 _ 021 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: MreifxetrurreedctoonatasinPiFnOgSp,erFfCi-u9o5r,ooocrtaansesauclfoomnpaotneewnhticohf mFCa-y30a0ls3o. b(e1Octanesuifonic acid) (CAS # 2795-39-3). Remarks: is FC-3003. CTuhrere3ntMipnrfoodrumcattiioonn ilontdincuamtebseritiwsaasmnioxttugrieveonf. 1T%hePFteOsSt,sa1m5p%le doicteytlhyslulefnaeteg,ly1co%l sbuotdyiluemthdeerc,yl79s.ul3f9at%e,wa0t.e0r,6%2%soSduilutmonlaeufryolamseurlf,at1e.,5a%ndsodium 0.05% benzotriazole. Tchhaerafcotlelrowiiznegdscounmcmeantrryataipopnlsieosftiompaumriitxiteusr.e Dwaittah imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical componentof the test sample. METHOD: Method: OECD Guideline. Type: Acute static range-finding study GYeLaPr:coNmopleted: 1997 Species: Pimephales promelas SAnuaplpyltiiecra:l AmqounaittiocrBiinogs:ysTteemmpse,raIntcu.r,eF,orptH,Colalnidns,DOC.O Exposure period: 96-hours Statistical methods: Not given. Test fish age: Juveniles Length and weight: (128-132 days) Average length = 23 mm Loading: 0.2936 g/L Average weight = 0.09786 g (wet) TPeresttrceoantdmietnito:nsN:one DDiilluuttiioonn wwaatteerr:chCeamrbiosnt-rfyi:lteNroetd gwievlelnw.ater (3M Well #2, St. Paul, MN). Lighting: Stock and Not given. test solution preparation: Thetest material was added directly tCoontcheendtilruattioinownastdeorsoinnga weight/volume rate: Once. basis. SEtxapboilsiutryoefvtehsseetless:t c1h.2emLivceaslseslolsuctoinotnasi:ninNgot1.n0otLetde.st solution. -- Number of replicates: One, Bk control duplicated Number Number of of fciosnhcepnetrrraetpiloincsa:te:th3r.ee plus 2 blank controls Water chemistry during the study: 002732 pH range (0-96 hours): 7.98.2 (control exposure) Temper7a.t7ur7e.9ra(n1g0e00(0m-g8/6Lhoeuxrpso)s:ure1)8.5 C Dissolved oxygen range (0-96 hours): 7.88.5 mg/L (control exposure) 4.58.6 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control, 10, 100, and 1000 mg/L Element values: 96-hour LCso = <1000 mg/L but >100 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test tShuebsftluaonrcoechReemmicaarlkpsrfoipeoldr.tiTonhealvoanleu.es reported apply to that mixture and not CONCLUSIONS T<h1e00t0esmtgs/aLm.plTehe961-0h0o0urmLgC/sLofeoxrpfoastuhreeacdmauisnendo6w6w%asmordteatleirtymiwnheidletothbee100 mglL exposure had no mortality. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY. eRexlpioasbiulrietyc:onKcieinmtirsacthiornasnkainndg t=es3t. oTrghaenisstmusd.y iSs aamrpalngeep-ufriintdyinwga,swintohtlpirmoipteedrly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES TSth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMR1C3o3m1p,an1y99,7.Environmental Laboratory, Oe THE-- R. -- ee ------------------------ Last changed: 6/27/00 002733 ENVIROMENTALLABORATORY " R1331 96-HR ACUTETOXITC OFI ATT HEAYD MINNOW, PIMEPHALESPROMELAS TYPETEST: STATIC x _STATICRENEWAL restasstace: FC-3003 AFFE (Austradia) OLUTONWATER: CARBO FILTEREDWELL WATERGMWELL 07,07 paul,ty Temp.____"CpH __, mg/L HLofg Raves FINDER TESTRSHSOURCE: Ag afic RSHAGE: F-13232 NO.OF FISWTEST CHAMBER: Gio Systems oatenecveo: 2-18-97 AVGWT0009O4T R9 AVG.SZE: 23 103 em 3 LOADINGRATE: 0.2936 TEST SOLUTIONVOLUME: O06 DATEINTATED:_4{ - 14 r-o]eard TOT r SIZE OFTESTVESSELS: DATETERMINATED: L1 9 woman 124 er 1130 Tes [2a] [of8ofte[o[50 7910Q [S145]1 [2[%.1] 2A[10[lo [oftefeefefreligiO (Ba1E2 |Lealty (00 1 |__| J ooo too ---- [|[ _ | |_| _|__|_ | -- 1 EE 1 1 rrr TTTTe) [BAY Cr 2 AE [DATE /ANALYST 4-15-21 log Tks 4-16-97 Ts LC50 (66% C1) "HaQ <locomglL la3y anBy | ean H-18-a7 o1% e] COMMENTS Th Topol08ASof nPuRgYmisihoestccoonnge dlee; aked 17 -Sampample somerseaSt e De ANALYST: Tks BEST COPY AVAILABLE + 002734 Environmental Laboratory Datwe:: _+R-171-39a71( Initials: PSaegse of 2 96-Hour Acute Toxicity To Fathead Minnow, Pimephaies Promelss Loading Rate Data Sheet Y Test substance: FC-- 3003 AFFE_( AUSTRALIA) [ [Fi1 sh T|oWeioghta,(g])] Laengyth,(em)| 1 : [2 Too%az| 1& | v [Bs TomaszI as 1 x [4 TounadTas | 4 [[5 Ce TTToooosoixzI] a2o s || 3% [7 Too|e2e 2 s| [s [ooe3s| as | : [ [39 0 _[T oao oso a|ld a 22 e ed|| ` Leading RateCalculation ; Avg. wt. (9): 0.097% Std. Dev: 0046S No. fishivessa,__3 Vol.vessel: _l1& 3 0.293589. fish_L__L Loading rate: 0.2936 002735