Document 1yd6vGp687XKgjDQ7dDYrxz65

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-203C and L-5440. (1-Octanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafploutoarsos-i,um salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was not noted. The test sample isFC-203C and isreferredto by the testinglaboratoryas "81-1/8". CurrentinformationindicatesthatFC-203C isa mixtureof 1.47% PFOS, 60% water,30% diethyleneglycolbutylether,3% sodium octylsulfate,3% hydroxy foamer, 10% polyoxyethylenenionooctylphenylether,0.12% sodium laurylsulfatea,nd 0.05% benzotriazol. The followingsummary applies to a with incompletely charecterfzecf concentration of impurities.Data may not accurately reflecttoxicity of the ffuorochomical component of the testsample.. METHOD: Method: EG&G, Bionomics protocolentitledumethods forconducting flow-throughtoxicittyestswithfreshwaterfish. Test type:Flow-throughacute GLP: No Year Completed: 1981 Species: Pimephalespromelas Supplier:Commercial supplieirnNebraska Analyticalmonitoring:Temperature,pH, DO, and conductivity Exposure period:96-hours Statisticamlethods: LC5ovaluescalculateudsingthecomputer program (createdby Stephan, 1978) based on the moving average angle analysis, probitanalysis,or binomialprobabilitays appropriate. Test fishage: Not noted. Length and weight: Length = 33 - 54 mm witha mean of41 mm Weight = 0.25 - 1.53g witha mean of0.57 g Loading: 0.38g/L Pretreatment:Not noted. Test conditions: Ollubon water: Well water supplemented withTown ofWareham untreatedand unchlorinatedwellwater. Dilutionwater chemistry: Hardness: 22 - 24 nig/L(as CaC03) Alkalinity:18- 24 Mg/L(asCaCO3) Conducgvity: 90 pmhos/cm pH: 6.7-6.8 LighfingC:oolWhite@)fluorescelnitghtpsrovideda dailyphotopedodof 16 hours lightand 8 hours dark withan intensitoyf30 - 70 footcandles. Stock and testsolutionpreparation: A primarystocksolutionof780 rng/mL was prepared by adding 3120 granisof testsample to distilled waterand dilutintgo4000 mL. Alltestsolutionswere prepared by a dilutedreliverinfgivenominal concentrationsofthe testsample and controlwater. Flow-through rate: Approximately 6.3 volume replacements oftestwater every 24 hours. Stabilityof the testchemical solution:Not noted. Exposure vessels: 39 x 20 x 25 cm glasstanks which maintainedtest solutionvolume of 15 liters. Number of replicates:two Number of fishper replicate:10 Number of concentrations: 5 plus a blank control Water chemistry during the study: pH range (0-96 hours): 6.9- 7.1 (control) 6.8- 7.2(2000 mg/L) Temperature range (0-96hours): 21 - 220C (forallaquaria) Dissolved oxygen range (0-96hours): 8.2- 9.1mg/L (control) 6.4- 8.4mg/L (2000 mg/L) RESULTS Nominal concentrations: Bk control,125, 250, 500, 1000, and 2000 mg/L Element value: 96-hour LC5o = >2,000 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe test substance remarks field.The valuereportedappliesto thatmixtureand not the fluorochemicalportionalone. CONCLUSIONS The FC-203C 96-hour LC5o forPimephales promelas was determined to be >2,000 mg/L. The highestconcentratiotnested,2000 mg/L, induced only5% mortality. Submitter3:M Company,EnvironmentLaalboratorPy.,O.Box33331,St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:Jimischranking2. This studymeets allthecriterifabrquality testing.However, thereisno analytica(lorother)informatiotnoconfirm the dilutewras workingpropedy. The studylacksinformatioonn the purity ofthetestsubstanceand lacksactualmeasurements of theconcentration offluorochemicailnthe solution. REFERENCES Thisstudywas conducted by EG&G, ofWareham, MA atthe requestof the 3M Company, 3M Lab Request number 7038,1981. OTHER Last changed: 8/26/00 E,4r L R PC.70-y3 ACUTE TOXICITY OF 81-1/8 TO FATHEAD MINNOW (Pimephales promelas). TOXICITY TEST REPORT SUBMITTED TO 3M ST. PAUL, MINNESOTA. REPORT@@#BW-81-10-1030 EG&G, Bionamics Aquatic Toxicology Laboratory 790 Main Street Wareham, Massachusetts October, 1981 INTRODUCTION The purpose of this study was to estimate the acute toxicity of 81-1/8 to fathead minnow' (Pimephales promelas) under flow-through conditions. Preliminary range-finding static tests and a subsequent 96-hour flow-through test were conducted at the Aquatic Toxicology Laboratory of EGSG,,.Bionomics, Warehamj, Massachusetts. The acute toxicity of 81-1/8 was measured by estimating the LCSO value for the compound at each 24 hour interval of the test.- The'LC*50 is. defined as the concentration.of the test compound in dilution water which caused mortality of 50% of the testr-animal population at*-the stated exposure interval. The flow-through test was conducted from 24-28 September 1981 with a two day pre-exposure period. All raw data generated are stored at the above location. MATERIALS'AND METHODS Procedures used in this acute toxicity test closely followed those described in-the EG&G, Bionomics protocol entitled "Methods. for conducting flow-through toxicity tests with freshwater fish." Values are reported to different levels of significance depending on the,accuracy of the measuring devices involved in any one process.. The 81-1/8, a brown colored liquid, was received from 3M, St. Paul, Minnesota on 12 August 1981. The test material was stored in the dark at ambient temperature (210 C). The material was tested as 10.0% 2 active ingredient. Nominal test concentrations are reported as milligrams of 81-1/8 per liter of test solution (mg/t). The-fathead minnow (Bionomics lot #8lAl7) were obtained from a commercial fish supplier in Nebraska and held in a 500-1 fiberglass tank.-under a photol5eriod of 16 hours light and 8 hours darkness. All ish were fed a dry,,pelleted food,,ad libitum, dai ly except during the 48 hours prior to testing.. There was i.0% mortality in the test fish population during this 2 day period (Daily Record of Fish Holding Conditions). The well water which flowed into this tank was characterized as having total hardness and alkalinity ranges as calcium carbonate (CaCOs) of 22-24 mg/L and 18-21 mg/j, respectively and a specific conductance range of 80-90 micromhos per centimeter (Umhos/cm) (Weekly Gravity Feed Tank Water Quality Analysis L'Ogbook). Other parameters monitored in the holding tank were a pH range of 6.5-6.7, a dissolved oxygen (DO) range of 94-99%--of saturation and a flow rate of 18 tank volume replacements/day (Weekly Record of Fish Holding Water Characteristics).- Test fish were,mainta.ined under these conditions for a minimum-of 14 days. The'-temperature in the holding tank was 210C during this 14 day peiriod,(Daily Record of Fish Holding Conditions). The specific conductance was measured with a YSI Model #33 salinity-conductivitytemperature meter and probe, the pH was measured with an Instrumentation Laboratory Model #175 pH meter and combination electrode, the-DO was measured with a YSI Model #57 dissolved 3 oxygen meter and probe and the temperature was measured with a Brooklyn alcohol thermometer. Total hardness and alkalinity were measured according to APHA et al. (1975). The exposure system used in this study was a modified, proportional diluter similat to that described by Mount and Brungs (1967) with a 0.50 dilution factor.. The dilution water was well water which was pumped to an epoxy coated concrete-reservoit where it was supplemented with Town of Wareham untreated and unchlorinated well water and aerated. This water was characterized as having total hardness and alkalinity ranges as CaC03 of 22-24 mg/L and 18-24 mg/t, respectively, a pH range of 6.7-6.8 and a specific conductance of 90 umhos/cm (Weekly Gravity Feed Tank Water Quality Analysis Logbook). The diluter delivered five nominal concentrations of 81-1/8, 2000, 1000, 500, 250, 125 mg/i and control water containing no . 81-1/8 to duplicate test aquaria. Each.glas-stest'a-quraium measured 39 x 20 x 25 centimeters (cm) with 19.5 cm high drains which .maintained water volumes of 15 1. The diluter delivered 0.5 1 of test water to each aquarium at an average rate of 188 cycles per day. This is equivalent to 6.3 aquarium volume replacements per 24 hour period. Illumination was provided by Cool WhiteR fluorescent lights centrally located above the test aquaria. Sixteen hours of light'at 30-70 footeandles at the water surface were 4 provided each day. Test temperatures were controlled by immerSion coil heaters in a water bath where the test aquaria were located. A 4-1 glass Mariotte bottle.in conjunction with a "dipping bird" delivery syste@d (Lemke,,et al. 1978) was utilized to deliver a stock solution to the mixing chamber of the diluter with every cycle. The stock solution of 780 mg/mt was prepared by adding 3120 grams,of 81-1/8 to distilled water and'diluting to 4000 mi. The 81-1/8 stock solution was introduced into the diluter and .Cycling began on 22 September 1981. The test was initiated two .days later when ten fathead minnow with a mean (range, N=30) wet weight and total length of 0.57(0.25-1.53) grams and 41(33-54) millimeters (Fish Weights and Lengths Log) were randomly distri: buted to each aquarium. Biological observations of the'fish and observationq of"the physical characteristics of the test solutions were made and .recorded at 0, 24, 48, 72 and 96 hours. The pH, temperature and dissolved oxygen (DO) concentrations were measured in both replicates of the control, high, middle and low concentrations at 24, 48, 72 and 96 hours and in both replicates of all concentrations at 0 hour of exposure. 5 The concentrations tested and the corresponding mortality data derived from the toxicity test were used to estimate 24-, 48-, 72- and 96-hour median lethal concentrations (LC50) and 95% confidence intervals. The computer program utilized (Stephan, 1978, personal communication) estimated LC50 values using one of three statisti6al methods in the'f6ll.owingorder of preference: moving average angle analysis, probit analysis, binomial probability. The method selected was determined by'the characteristics of the data base (i.e. presence or absence of test concentrations causing mortality of 100% of the test animal population, test concen-' trations causing mortality of a partial number of animals in the population, etc.). The computer program scanned the data base, idetntifiedthe most preferred statistical method and performed the analysis. RESULTS The test material 81-1/8, was not acutely t-oxic to@fathead minnows at nominal.concentrations as high as 2000 mg/L (Table 1). Al.though there appeared to be no.81-1/8 related mortality, fish exposed to 2000 mg/.t 81-1/8 were lethargic after 48 hours exposure. Fish exposed to 1000. 500 and 250 mg/i 81-1/8 also appeared lethargic at 72 hours exposure. The estimated LC50 values after 24, 48, 72 and 96 hours of exposure was >2000 mg/l, the highest concentration tested. 6 The temperature in various*aquaria ranged from 21-220C during exposure. The pH and dissolved oxygen concentrations measured during the toxicity test are presented in Table 2. LITERATURE CITED APRA, AWWA, WPCF. 1975. Standard methods for the examination of water and wastewater. 14th Edition, Washington,, D.C. 1193 pp. Lemke, A.E., W.A. Brungs and B.J. Halligan. 1978.. Manual for construction and operation of toxicity-testing proportional diluters. EPA-60D/3-78-072.. Mount, D.I. and W.A. B rungs. 1967. A simplified dosing apparatus for fish toxicology studies. Water Res. 1: 20-29. Stephan, Charles. 1978. U.S. EPA, Envirormental Research Laboratory, Duluth, Minnesota. Personal communication. Table:l. Concentrations tested, corresponding percentage mortalities and behavioral observations of fathead minnow (Pimephales promelas) exposed to 81-1/8 under flow-through conditions.. Twenty fish were exposed to each concentration. Nominal. concentration (mg/z) 2000 1000 Soo 250 125 control 24 hour 0a 0a a 0 0 0 0 Percentage mortality 48 hour 72 our 0 a,b 0a 0a 5a a 0 0 0 a,b,c 0 a,b 0 a,b 10 aob a 5 0 a Suds formed on.the surface of the test,--soluti6,n. b Some fish were lethargic. c One fish was at the surface of the test solution. 9 C-Eo-u-r 5 ab 0a 0a 10 5 5 Table 2. The pH and DO concentrations measured during the 96-hour flow-throu test with fathead minnow (Pimephales promelas) and 81-1/8. The ran ments made from replicate aquaria are reported. Nominal concentration (mg/.t) 0 hour 24 hour 48 hour 72 hour .pH 2000 1000 500 250 125 control 7.2 7.2 6.8 6.8 7.1 -a -a -a 7.1 7.0 6.6-6.8 6.4-6.9 7.0 -a -a 7.1 6.9 6.7-6.9 6.9 7.1 7.0 6.9-7.0 7.0 DO (mg/k) 2000 1000 8.3-8.4 7.6-7.9 7.9 8.2-8.3 a a 500 8.2-8.4 7.4-7.6 7.1-8..l a a 250 8.7 125 8.7-8.8 7.6-7.7 8.1.-8.5 cont.r.ol. .........9...0.-.9...1.......8..2.-.8...4......8...3.-8..5.. .. 7.0-7.4 a 5.1-7.8 a 7.7-7.8 8.2-8.4 a No measurement made. SUBMITTED BY: EG&G, Bionomics Aquatic Toxicology Laboratory 790 Main Street Wareham,.Massachusetts October, 1981 PRINCIPAL INVESTIGAT OR: Brenda P. Wilson Aquatic Biologist STUDY DIRECTOR: Gerald-.A. LeBlanc #Catic Le Toxicologist DATA AUDITED BY: Robert E. Bentley Director, Quality ssurance Unit