Document 1xk4B29dyVnr3BRQXQGvY2oX
AR 226 _ 329]
473
_-- ~
TRADE SECRET
AR226- 329] wDupoonwt 13s347
Study Tie H-25914: Static, Acute, 96-Hour Toxicity Screening Test with Oncorhynchus mykiss
AUTHORS: Laurie D. Bouchelle Robert A. Hoke, Ph.D.
STUDY COMPLETED ON: August 1, 2003
PERFORMING LABORATORY: HEaLskdelulPLoanbtodreatNoermyofuorrsHeaanldthCaonmdpEannvyironmental Sciences
--_
NEelkwtaornk,RoDaedl,awP.a0r.e B1o9x71540-0050
LABORATORY PROJECT ID: DuPont-13347
Company Sentzed. Boss not comin 15 cy
Patol
~~
SSTcHor2e5m9ci1en4ig: TSesugc,wiAw hcOie,ni6nHoh urTpoOtcs eomprchrpkpDwui peonwti1s3s34e7 ~
CERTIFICATION Wfreo,mtthheisunsdteurdys.igned, declare that this report provides an accurate evaluation ofdata obtained
workcompitsavy: _{ApuuiteDBTAoncucoeuhBSaoolinelise
Ldeggata2o000:3
et Suiy ir BSrckResRoAoberBtoAfo.nocskbieoFgiit ad Mier Ge=stes
_
Company Santzed. Doo not contain 75 Ga.
EE
------
~
STUDY INFORMATION
subse To [RIN C( T -- Synonyms/Codes: + H-25914 1
Submitter's Notebook Number(s NNN.)
Haskell Number: 25914
Keown puis
} BN Physical Characteristics: Amber liquid
Stability: The test substance appeared to be stable under the
conditions of the study; no evidence of instability was observed.
hE - Sponsor: E.L du Pont de Nemours and Company Wilmington, Delaware 19898
Study Initiated/Completed: July 21,2003 / (sce report cover page)
Company Saniized. Boss not contain Ts cay
ScHSr-ce2re5e9en1ni4in:nggTTeSetsasttticww,iitAthchuOOtnnecc,oo9r6rh-byHnnoocuhhruusTsommxyipckkiitssyss ___ --
DDuubpomnten13s34s7r
SUMMARY
The acute unaerated,
toxicity ofH-25914 96-hour, static test.
to
the
rainbow
trout,
Oncorhynchus
mykiss
was
determined
in
an
Tmheeansttuedmypewraastucroendoufc1t1e.d5wCit(hra4ncgoencoefntr11a.t3i-o1n1s.o9fC).H-2O5n9e14tesatncdhaamdibleurtiwoanswuasteerdpceonrtrtoelstat a
cwoantceernctornattrioolnawnidthth5et0e.s1t,or1.g0a,n1i0smasndin1e0a0chmgc/haLmtbeesrt.conBcaesnetdraotniovnissuwaelroebcsleeravratainodnsc,oltohreldeislsuwtiiotnh
0 precipitate exposure.
at
test
start.
All
water quality parameters
were
within
acceptable
limits
during
the
E0.x1p0o,s1u.r0e,of10r,aainndb1o0w0tmrogu/tLtoretshueltdeidluitnio0n, w0a,t0e,r0,coanntdrol10a0nd% nmoormtianlailtyH,-r2e5sp9e1c4ticvoelnyc,enattrtahteioennsdooff o9r6ghaonuirssm.s.NoThmeorhtiaglhietsytonromsuibnlaelthcaolnecfefnetcrtastiwoenrceasueseinnginnothemodritlaultiitoynawtatteesrt ceonndtrwoalste1s0t mg/L. `HT-h2e5l9o1w4esctonncoemnitnraalticoonnscewnetrreatuisoendcfaourscianlgcu1l0at0i%onmoorftLalCistyvaatluteesst.end was 100 mg/L. Nominal The results are summarized as follows:
~ N96o-mhionuarlLcConsce(n9t3r.at7i%oncsoonffidHe-n2c5e91i4n,termvgal/)L|| d3i2lu(t1i0ontowa1t0e0r)control, 0.10, 1.0, 10, and 100 for H-25914, based on nominal concentrations, mg/L
tHe-st25u9si1n4getxhheibriatiendbmowedtriouutm, cOonnccoerhnyfnocrhauqsuamtyikcishsa.zard in an unaerated, 96-hour, static, acute
Company Sanitized. Does not contain TSCA CBI i
rS2c5t9eee1n4i:mng TSoetsattivcw,iitAhbcuOOtnwec,oo9rr6hp-ynHnoccuhhruasTommxbiicssistsy. --
MATERIALS AND METHODS
DDwuPoownta13s3a47r
A. Test Solution Preparation nTeosmtinsaulbsctoanncceentsroaltuitioononfstweesrtespurbesptaarnecde bsyolduitrieocntswadedriteionnotofadHj-us2t5e9d1f4ortopudriiltuyt.ioTnewsattesro.lutTihoens cwoenrteaipnreedpa1r5edLobyfaHdadsiknegllthLeabaoprpartooprriyawteelalmwoautnetroafntdessttisruribnsgtafonrceaptporeoaxcihmattesetlych3a5mmbienrutwehsi,chTest solutions were clear and colorless with no precipitate at test start
B. Dilution Water
aDnildutseiaolnewdaitnetroobreidgrioncakt.edTfhreomhatrhdenHeassskoefltlhLeabwoerlaltwoartyewreilslawdhjiuscthedist3o7a8p-pfreoetxidmeactpelaynd is cased
a1e0r0a-te1d4,0mpags/sLedatshCroauCgOhsagbryeethnesaflnodw-fpirltoeprotrotiroenmeodvaeddiirotni,onanodf CfialCtelr.ed Tthhreouweglhl5w0-a,te1r0-i,s atnhden
d0i.s4tr5ipbumtefdiltehrrsoutoghreamgeodvepoplayrvtiicnuyllatcehsl.orTihdeepwiaptinegr.isThheeatdeidluotricohnilwlaetdearsisaapnparolpyrzieadttewaincde yearly
--~
for major anions and cations, metals, total organochlorine and organophosphate pesticides, and
polychlorinated biphenyls. The dilution water meets OECD" and ASTM criteria and
specifications.
C. Test Organism Culture
-
CRaaliifnobroniwa.troTuht,eOfnicshorwheyrnechheulsd miynkais2s7,2-wLe,reciprucurlcahra,sfeidbefrrgolamssThhoolmdaisngFitsahnkC(o8m4p-acnmy,diAanmdeteerrs,on,
74-9d-acympdeerpitohd)pfroerce3d2idnagytsheintecsot,ntwiantueorustleym-pfelroawtiunrgeswerlalngweadtefrroam
Haskell 11.0 to
Laboratory. During the 11.8C (mean 11.3C).
`The fish were fed freshly-hatched, live brine shrimp (San Francisco Bay Brand, Inc.) and
wwAeeqreuekadnMaoyatsxpraenSsdteanortnteciren Ffdiiansihgleyirnloitnnhgeweh3eo0kl0ediSnndDgs0t3aann(dkPhnMooIlridiNanuyttshr.eitSfiiioscnhknIunestseesdr,nfaiotnrijoutnreaysl,t,ianLng.LdCvL)iessitsbwlitecheaabndna0oir.lmy5a%olnities
tmhoerthaollidtiynwgatsanskesenanddurtienstgcthhaem4b8erhso.urs prior to use for testing. Fish were identified by labels on
D. Test Methods
nFoomuirnnaolmitensatlcotnesctenctornacteinotnrsatwieornse a0.n1d0,ad1i.l0u,t1i0o,nawnadte1r00comntgr/oLl
were used H-25914
in
this
study.
The
~
Test chambers approximately
w15erLeosfttaeisnltessoslsutteieolna(q2u6a-riLam[a3x0i(mleunmgtvh)olxu3m0e;(w1i7d.t5h-)cmxn
30 (height) cm] which held test solution depth). One
-
------
`Company Sanitized. Does not contain Tara cn
1Sc2r5e9e1n4i:ng TSetastticw,iAtchuOtnec,o5r6h-yHnocuhrusTomxyikciistys
Dupont 13347
~~ Eteascthchcahmabmebrerwawsasusceodvepreerdtewsit tsohalugtliaosns(ptloattaelofto prteevsetncthtahmebefrissh;fSrofimshesicnapciancgh. test chamber).
Acodmdpilteitoendo.ffMiosrhtatloitthyeatnedstbseohlauvtiioornaslwoabsseirnvitaitaitoendsawfteerremimxaidnegoefvterhye2t4eshtosuorlsutainodnsatwtaesst end. Dweeraedtfhieshabwseernecereomfoovpeedrcfurloarm mthoevteemstencthaamnbderlsacdkuroifnrgeadcatiiloynotbosegrevnattlieonpsr.oddCirnigte.riAatfotresdteath conclusion, all fish were humanely sacrificed using MS222 (3-aminobenzoic acid ethyl ester). Athere9c6i-rhcouluartitnesgtwaattaeprpbraotxhimwaatseluyse1d1.t5omCaiwnittahian rmaenagne otfem1p1e.r3attour1e1i9ntCh.e tAestphcohtaompbeerrisodduorfing i1n6chlouuderds 3li0ghmtin(uatpepsrooxfimtartanesliyti2o6na7ltloi4gh9t7(7L2uxt)o a1n5d9 8Luhxo)urpsredcaerdkinnegssanwdasfoelmlpolwoinygedthweh1i6c-hhour light interval.
mDeiasssoulrveemdenotxsygweenrecotnackeentnrbaetfioorne, pfiHs,h awnedreteamdpdeerdataturteeswtesrtaertm,eeavseurrye2d4ihnoaullrstetshtecrehaafmtbere,rsa.ndTahtese
test end. Test `manner at test
senodl.utions
were
not
aerated
during
the
test
and
were
disposed
ofin
an
appropriate
E. Results and Conclusion
~ 0E.x1p0o,s1u.r0e,of10r,aainndb1o0w0tmrogu/tLtoretshueltdielduitnio0n, w0a,t0e,r0,coanntdrol10a0nd% nmoormtianlailtyH,-r2e5sp9e1c4ticvoelnyc,enattrtahteioennsdooff
o9r6ghaonuirssm.s.NoThmeorhtiaglhietsytonromsuibnlaelthcaolnecfefnetcrtastiwoenrecasueseinnginntohemodritlaultiitoynawtatteesrt ceonndtrwoalsfe1s0t mg/L.
Tlohaedilnogwewsetignhotmifnoralthceonfcisehntursaetdioinn cthaiussitensgt
100% mortality at was 0.2805 g/L.
test
end
was
100
mg/L.
The pretest
`The 96-hour LCs for H-25914, based on nominal concentrations, was 32 mg/L. H-25914 ethxehirbaiitnebdomwetdriouutm, Ocnocnocrerhnynfcorhuasqumaytkiicshs.azard in an unaerated, 96-hour, static, acute test using
-- Company Sanltized. Doesnotcontain TSCA Cat memeber
SHcer2e5e9mn1i4n:geTSetsitchw,itAhcOuOtnenc,oc9r6h-oyHnocruhrusTomxyibkciilstsys --
DDuuoPomnits13n34r7
REFERENCES 1. TOersgtainnigsoatfiConhefmoircEalcso:no2m0i3c,C1o7-OJpuleyra1t9i9o2.n and Development (OECD). Guideline for the
2. A`CmoenrdiuccatinnSgoAcciuettyefToorxTiecsittiynTgeasntsdwMiattherFiiaslhses(,AMSaTcrMo)i.nve(r1t9e88b)r.ateSst,anadnadrdAmGpuhiidbeiafnosr. E 729-88a. Annual Book ofASTM Standards, Vol. 11.04,
3. ESvtaelpuhaatni,onC,.EP.ro(1c9e9e7d).ingMseotfhothdesFfiorrstcAanlncuuallatSiyanmngpoLsCissu.m oAqnuAaqtuiactiTcoxTiocxoilcooglyogayn,dAHSaTzaMrd Special Publication 634 (F.L. Mayer and J.L. Hamelink, Eds.) pp 65-84. Philadelphia.
4. uSnmdrecrheTkS,C1A,:Clmeemtehnotds,s aR,n,dMeovraclouactki,onRo.,fdaantda.RaEbnevri,roWn.m(e1n9t9a3l).ToAxsisceoslsoignygaencdolRoigsikcal hazard AAsmseersiscmaenntS,ocAiSetTyMfSoTrPTes1t1i7n9g.an(dW.MGa.teLrainadlsi,s,Ph1.i5l.adHeulgphhieas., and M.A. Lewis, Eds.), pp 22-39.
--~
~~ _--
`Company Sanitized. Does not conntaineTSCeA Ca
SHc-r2e5e9n1i4n:g TSetastticw,itAhcuOtnec,o9r6l-yHnocuhrusTmoyikciitsys --~
DuPont 13347
TABLE |
DISSOLVED OXYGEN ANDSpOHLUOTFITOENSSTAAMNPDLEDSILUTION WATER CONTROL
Nominal H25914 Concentration mg Dilution Water Control| 0.10
10 10 100
Test Initiation(0 hours)
Dissolved
Oxygen
(ng
p
10.8 107
73 73
105
73
106 106
73 73
Test Completion Dissolved Oxygen
g/L) 86 85 89 8.4 9.4
(96 hours
p 73 73 73 73 74
a Measurement performed at 24 hours due to oil mortality.
~
TABLE2
MORTALITY OF Oncorh9y6n-cHhuOsURmyTkiEsSsTINWIATNHUHN-A25E9R1A4TED, STATIC, ACUTE,
Nominal H-25914 Concentration (mg
|24Hous
Dilution Water Control os
010 10
0s os
10
os
100
sis
NumNbuemrbaetrStDuedyadS/tart a8Hows | 7Hous | 96 ows]
05 os
os os
[ os
os 0s
0s os
0s os
sis
E24
sis
--~
-
----
ee
`Company Sanitized. Doesnotcontain TGA Ci: