Document 1gkEgmOREYoJbrnKeQw6OVz2j
Wildlife International, Ltd. a
Project Number 454-101
PFOS:
WIATH96T-HHEOSURALSTTAWTAITCEARCMUYTSEITDO(XyIsCiIcToYpeTsEaStTic)
ADDENDUM
`WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A-101 3M LAB REQUEST NO. U2723
`OPTS Namber 850.1035 U.S. Environmental Protection Agency
Series 850 - Ecological Effects Test Guidelines
"This studywasreportedusinga ts substancepurity f 0.49%.The estsubstance `characterization was revised on 9/7/00andthepuriwtays determined to be 86.9%. The Sponsor
requested that the results be revised based on the most recent purity analyses. The results previously presented were simply corrected by the ratioofthe two purities or a factorof0.9603.
No atemp was made to eclculste anlyical standard concentrationso measured ences refvriosmeodrisguinmamlarraywpadagteaf.rAosmtshuechf,inmailnroerpoerrtraornsddtuheetmoorsoturnedcienngtmcaerytibfiecaptreeosefnta.nalAytsitsa.ched is the
= 38
w 77
Wildlife International, Ltd. an
Project Number 454-101
REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9%
SPONSOR:
'SPONSOR'S REPRESENTATIVE:
DALoTcAATAINODN AOFCSOTPUYDOYF, RHaEW
FINAL REPORT:
LITDB. LRFOTCRNAMTZEIRO:N
3M Corporation Ms. Susan A. Beach
Wife Intemational Lid.
Easton, Maryland 21601
ssaaton
TEST SUBSTANCE:
stu.
PROS: A.9Sst6icAHceTooxicuity Trest with the PFOS (Perfluorooctane Sulfonic Acid PotassiumSalt)
SaltwaterMysid (Mysidopsis bahia)
MCEOAnNBMTERAASTUIROENDSTEST
TEST DATES:
Leo oFTEST:
NegativeControl;0.55, 11,18,29and 5.2 mgai/L
Experimental Start - March 8, 1999
BEixopleorgiimceanltaTlerTmeimnaitniaotno-nMaMracrhc1h2, 112,9919999
f
96 Hous
`TEST ORGANISM:
`Saltwater Mysid (Mysidopsis bahia)
I
SOURCE OFTESTORGANISMS:
`Wildlife International Ltd. cultures
|
Easton, Maryland 21601
AGE OFTESTORGANS:
Suvaies
STOR LE
55%conFNCE ars: NomorTALTY
(CONCENTRATION:
Tsai 29 44madin
Llmgaill
'NCOo-NOCBRSTERRAVTEID-OENF:FECT-
ingais%
Wildlife International, Ltd. 3.
CERTIFICATE OF ANALYSIS
Project Number 4544-101
INTERIM CERTIFICATE OF ANALYSIS
Revision 1(9/7/00)
Centre AnalyticalLaboratorCiOeAs Referen#ce: 023-0184
i
3M Product: PFOS, Lot 217
Referenc#e: SD-018
i
urity: 86.9%
.
P oo eon
[ IdentA ificap tion p7 e] aEr"Ea ~E~n Wmheic teCoe ystallimnePaowder:| |ir1 Conforms |
Metal1.s (CIaClPcMiuSm)
2.
3.
SMaogdnuemsium
4. Potassium'
56.. Nliocnkel
7._Manganes
"Total % Impurity
F Berere LEee 1 osm
[ beiang E
pbeol f lle 23. 0L00a1 wwe/nwts%
a |: a
oo | 4 689 wAn% | s oolwmw
[eaimmint: | ooswnn
Ernie
0] 7 000i win
Ere
TT Salman
("GToOtaMlS%)Impurity
[Er
Related Compounds -- ||
POAA
[Residual Solvents (TGA)|
ae
=
aE LE
neon oe
RE Ral
=""ie= 0077
NoneDetected
0.33wt/wt.%. NoneDetected |
Tnorg1.aniCchAlnoiroidnes 00)
2. Fluoride 3 Bromide
45.. NNiittrriattee 6. Phosphate Ck7._Sudlefaste' 00 2 PFTPFAA
3. HFBA
`Elem4.entaNlFAPnAaly:
[Dea e em e sel L
S La ailnma
a] a
2 5
Si i Sa da E Sn S4
Lalas C6
Dae
ba
7.
LTEELa L s iC21
ee
3
Sie
Ea
4.
ooswumn
0svwm% Gam
<o0.w00e9wwihmn% <orwaen t876ewhnwt% oQllwwmmn
oon
028 wt/wt%
1. Carbon 2. Hydrogen
34. NSuiltfruorgen 5. Fluorine
1. `TheoreticalValue = 17.8%
2. `TheoreticalValue =0%
34.. 5.
"TTThhehoreeetoiocrarlVeVVeaaaltlltuuueieei==c=c056a.0%a9%l5l%
11248 wt/w%t 2. 0.244 WW%
435.5sL471a4wwwteinat%
coans.018a
Page 10f3
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-0184 DateofLast Analysis: 08/31/00 Expiration Date: 08/31/01 Stofage Conditions: Frozen 0-10C Re-assessment Date: 08/31/01
"Purity = 100% - (sumofmetal impurities, 1.45% +LC/MS impurities, 8.41%+Inorganic
Fluoride, 0.59%+NMR impurities, 1.93%+organic acid impurities, 0.38%+POAA,
033%)
Total impurity from all tests = 13.09%
Purity = 100% - 13.09% = 86.9%
Potassium is expected in this salt form and is therefore not considered an impurity.
PobusreirtvyebdyfDorStChisissgaemnpelrea.lly not applicable to materials of low purity. No endotherm was
"iSnuolrfguarniinc tahneiosnammpeltehoadppceoanrdisttioonbs.e c`oTnhveeratneidontoreSsuOl,taagnrdeehsewnecleldweitetchttehdeussuilnfgurthe determination in the elemental analysis, ending confidencetothis interpretation. Based on the results, the SO4 is not considered an impurity.
TFA
HFBA NFPA PFPA
`Trifluoroacetic acid
Heptafluorobutyric acid Nonofluoropentanoic acid Pentafluoropropancic acid
"Theoretical value calculations based on the empirical formula, CsF17S0yK" (MW=538)
"This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160).
COA3-018A
Page20f3
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-018A.
LC/MS Purity Profle:
CC
mun.[wwe|
I 07350 Ol L : A
r oe e am
en Tea CTT lmTT IER
Ncoutrev:esT, hreespeCc4tiavneldyC.6ThvaeluCeSswvearlueecwaalcsuclataedulsicnguutsihnelg0ta4heatanvdeeCra6dgsetarnedsaprodncsaelifbacrtaotrison afvreormatgheerCe4spaonndseCf6acsttorasndfarrodmcutrhveesC.6 aLinkdeCwi8sset,atnhdearCd7cvuarlvueesw.as calculated using the
Prepared By:
Reviewed By:
David S. Bell
Date
Scientist, Centre Analytical Laboratories
John Flaherty
Date
Laboratory Manager, Centre Analytical Laboratories
conns01sA
Page3ar
ilCdOTlOiKfIeCOILnOGtYeArNAnLaYTtICiAoLTnESaTlIN,G SLERtVIdCE.S
September 27, 2000
3MsM. SCuosrapnorAa.tioBneach 9E3nv5irBounsmheAntvaleLraboratory P3OM. BBuiolxdi3n3g323-138-09 St Paul, Minnesota 55133 DearSusan
4547-10E2n,cl4o5s4eAd-p1l0e3aAs,e Sfi4nAd-A1d0d4,en4d5u4m4s-1f0o5r,W4il5d4l4i1fe06I,nte4mSa4tAio-n1a0l7,,Lt4d5.4PAr-o1j0ec8tnNdum4bSe4rAs-415049A-101, you have any questions, please letmeknow.
Sincerely,
\
KutR Drotuar
`Senior Biologist
Rogan
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