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ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acemtaaytea;lso be referredtoas PFOSAA, FC-128 oras themajor component of FC-1 27, FC-129, FC-1 09,or FC-109-X. (Glycine,N-ethyl-N[(heptadecafluorooctyl)sulfonpyolt]a-s,siumsalt,CAS # 2991 51-7) Remarks: The testsample isFC-127. Currentinformatioinndicateist isa mixtureof40-44% testsubstance,32% water,14% isopropanol4,% ethanol,and 6-10% C4-C7 fluoroalkyclarboxylatecompounds. The followingsummary isabbreviated because the testsample isa mixture of the testsubstance in a waterlorgank solventsolution. Data may not accuratelyrelatetoxicityof the testsample with thatof the testsubstance. METHOD: Methods: ProtocolReference EAR, 11/8/79. Test type:Not noted. GLP: No Year Completed: 1981 Test organism source:ActivatedSludge mixed liquofrrom St.Paul, 'MinnesotaMetro Wastewater Treatment Plant RESULTS Nominal concentrations: Bk control,1000 mg/L Element values: No acute inhibitoreyffecton activatedsludge respiratiornateat 1000 mg/L witha contacttime of 3.25 minutes. Remarks: Testingwas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarks field.The valuereportedapplies tothatmixtureand not thetestsubstance. No attemptwas made to determinethe impact ofthepresence oftheorganicsolventsorwhat portionofthe toxicitcyan be contributedtothe Perfluorooctanesuffonylamido(ethyl)acetate. CONCLUSIONS Limitedcontacttime estimatesthatFC-127 isnotexpected to inhibitthe .activiotfyactivatedsludge. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:Jimischranking3. Testinglacksrecordofmethodology used. The method was notan agency approved method. There isa lack ofcharacterizationfthetestsample. Test solutionsnotanalyzed fortest substance concentrations.Nominal valuesused. REFERENCES This studywas conducted by the3M Company, Environmental Laboratory,St.Paul,MN, Lab Request number 7009. OTHER Last changed: 5/18/00 3h Env Ironmenta I Labor4itory. -Ar'Ote.Tax Ic Ity.of A CWVO Syb t Niotoo S104 m 1 L I UeS Lab:-r" t no s - 1kc A iiIYs m e actIva ted s Itidgemi xed lqijoe* !6@.ir.*F p Metro Wastewater.P!Ant-ov Sample descrlptio'n' im: Preparation of stock sol4itt with deionized*rftaentWater.".Dat@ g.r.a= .@of saimo1e vier diluted to 14049 etp*@*t-!-6h: 93 :0000 'ml -TI ME min. sec. C NTH ION O@O;EST MIJERIA .@DI N ASTIV ;E@ SLUDGE g! RE mg@i mg/.l .@mg4 -1 1-con#t"61 mg /.Y- '7.kr 7-50--3o 7. !@r 10 3.60 .3 2 COMMENTS: 7 00 lop '301410 ENVIRONMENTAL LABORATORY SUMMARY SHEET. TEST: Acute Toxicity of a C4*opoundto Activated Sludge t4lxedLiquor. 9 71 SAMPLE DESCRIPT. F-6390 ANALYST: IJADF- A. !S@C4f-L) .7 T. --4 DATE ANALYZED: LAB REQ. NO.: '7009 filIltittlitliililiitIlilI l 7 Irk-1-.-1Ii 6 m E <5 LAJ C) C3 2 -4-- 0 4-- -4 7r I tr TI ME - minutes .5 b 7